BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,532,298	A→T	T15T (ACA→ACT)	femA_3 →	Aminoacyltransferase FemA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,532,298	0	A	T	100.0%	421.8 / NA	131	T15T (ACA→ACT)	femA_3	Aminoacyltransferase FemA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (20/111); total (20/111)

Only 100 of 131 total aligned reads displayed.

TTTCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACT  >  USA300TCH1516_ALE/2532155‑2532441

tttCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAAAGTTGACTGCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                            <  2:427232/149‑1 (MQ=255)

 ttCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                           <  1:900600/149‑1 (MQ=255)

 ttCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTCCCGTAAAAGGCGGAATGCGATAAAATGTCCTGCTGAGCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                           <  2:657205/149‑1 (MQ=255)

       gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                                     >  1:859327/1‑149 (MQ=255)

        tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTTAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGAATGAGTTCAATGCGTTTACTACAAATCAttttt                                                                                                                                    >  2:518026/1‑149 (MQ=255)

         gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                   <  1:275938/149‑1 (MQ=255)

         gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                   <  1:240704/149‑1 (MQ=255)

         gtATTTGTAAACAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGACCTTTGCAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                   <  1:312620/149‑1 (MQ=255)

                tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGTATAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                            <  2:538209/149‑1 (MQ=255)

                   tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                         <  1:364395/149‑1 (MQ=255)

                   tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGATTACTACACAGCATTTTTCACATTacaca                                                                                                                         <  1:481086/149‑1 (MQ=255)

                          ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                  <  2:430162/149‑1 (MQ=255)

                          ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                  <  1:156115/149‑1 (MQ=255)

                          ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTACAATTCAGAGAGGGATGGGATAAAATGAACCTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                  <  1:79093/149‑1 (MQ=255)

                            gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                                <  1:380168/149‑1 (MQ=255)

                                  ataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAt                                                                                                          <  2:316356/149‑1 (MQ=255)

                                   aaaaaataTAGCATTATTAATAAATTCAAATATTATAGTTCATTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTGCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCCtt                                                                                                         <  2:709664/148‑1 (MQ=255)

                                   aaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGTGATAAAATGAACTTTGTAACGTTGACTCCGGATGCGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAtt                                                                                                         <  2:701355/148‑1 (MQ=255)

                                   aaaaaaaaGAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAtt                                                                                                         <  1:431048/148‑1 (MQ=255)

                                      aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGCGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGAATGAGTTCAATGCGTTTACTACCAAGCATTTTTCACATTACACACAATCAGCTATTCATTACa                                                                                                      >  1:628266/1‑149 (MQ=255)

                                      aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTCCTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACa                                                                                                      <  1:411569/149‑1 (MQ=255)

                                      aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACa                                                                                                      <  2:312771/149‑1 (MQ=255)

                                        aaaTAGCATTATTAATAAATTCAAACATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGATTTCAAGGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  1:539227/149‑1 (MQ=255)

                                        aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTGTTCATTACAAt                                                                                                    >  1:907647/1‑149 (MQ=255)

                                        aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  1:427267/149‑1 (MQ=255)

                                        aaaTAGCATTATTAATAAATTCAAAAATTATAGTTAAATTCAGGGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  1:229766/149‑1 (MQ=255)

                                        aaaTAGCATTATTAACAACTTCAACAATTAGACTTCAATTCCGAGAGCGATGTGATAAAATGAACTTTGTAACGTTGACCTCGGATCCGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  1:539269/149‑1 (MQ=255)

                                           aaGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCAt                                                                                                 <  1:524325/148‑1 (MQ=255)

                                             gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                               <  1:770441/149‑1 (MQ=255)

                                             ccATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                               <  2:816777/148‑1 (MQ=255)

                                              cATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga                                                                                              <  2:88060/149‑1 (MQ=255)

                                                ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAACATAGAGt                                                                                            >  1:345860/1‑149 (MQ=255)

                                                   ttaATAAATTCAAAAATTATAGATCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAATACACACAATCAGCTATTCATTACAATCATAGAGTTGa                                                                                         <  1:173232/149‑1 (MQ=255)

                                                    taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAt                                                                                        <  1:237461/149‑1 (MQ=255)

                                                     aataaATTCAAAAATTATAGTTCCATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAtt                                                                                       <  1:456004/149‑1 (MQ=255)

                                                      ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGCAAAGGTAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTATTTACAAACATAGAGTTGAttt                                                                                      >  1:449238/1‑149 (MQ=255)

                                                       taaATTCAAAAATTATAGTTCAATTCAGGGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCCTAGAGTGGATTTa                                                                                     >  2:819360/1‑149 (MQ=255)

                                                       aaaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa                                                                                     <  1:712459/148‑1 (MQ=255)

                                                       aaaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa                                                                                     <  1:774255/148‑1 (MQ=255)

                                                        aaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAGTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                                    <  1:164329/149‑1 (MQ=255)

                                                          aTTCAAAAAGTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCGATGTATTACAATCATAGAGTTGATTTaaaa                                                                                  <  1:223688/149‑1 (MQ=255)

                                                             cAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                               <  2:652932/149‑1 (MQ=255)

                                                              aaaaaTTATAGTTCAATTCAGAGAGTGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                              <  2:149698/149‑1 (MQ=255)

                                                                 aaTTATAGGTCAATGCAGATAGGGATGGGATAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAACCCTTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                           <  2:64221/149‑1 (MQ=255)

                                                                   ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgtg                                                                         <  2:350963/149‑1 (MQ=255)

                                                                    tataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc                                                                        <  2:397363/149‑1 (MQ=255)

                                                                      taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                      <  1:46783/149‑1 (MQ=255)

                                                                          tCAATTCCGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  <  2:785143/149‑1 (MQ=255)

                                                                          tCAATTCAGAGAGGGATGGGATAAAATGAGCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  >  2:496455/1‑149 (MQ=255)

                                                                          tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  >  1:849302/1‑149 (MQ=255)

                                                                          tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  >  1:125218/1‑149 (MQ=255)

                                                                          tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCAATGTGCATGTTg                                                                  >  2:759801/1‑149 (MQ=255)

                                                                          tCAAGTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACCAGCAGAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  <  2:426222/149‑1 (MQ=255)

                                                                           cAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGt                                                                 <  2:116606/149‑1 (MQ=255)

                                                                             aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCCTTTTTCACATTACACACAATCAGCTATTCATTACAATCAGAGAGCTGATTTAAAACGCGATGTGCATCTTGtcg                                                               >  2:206670/1‑147 (MQ=255)

                                                                              ttCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                              <  2:251108/149‑1 (MQ=255)

                                                                              ttCAGAGAGGGATGGGATAAAATGAACTATGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGACTTAAAAGGCGATGTGCATCTTGTAgg                                                              >  1:563261/1‑149 (MQ=255)

                                                                                ccggtagGGATGGGATAAAAAGACCTTTGTAACGTTGTCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                            <  1:575305/144‑1 (MQ=255)

                                                                                cAGAGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                            <  1:315237/149‑1 (MQ=255)

                                                                                cAGAGAGGGATGGGATAAACTGAACTTTGTAACGTTGCCTTCGGATGTGTTCAATGTGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAGTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                            <  2:540096/149‑1 (MQ=255)

                                                                                cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                            <  2:358778/149‑1 (MQ=255)

                                                                                 agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTTCAAGCATAGAGTTGATTTGAAAGGCGATGTGCATGTTGTAGGGGt                                                           >  1:372628/1‑149 (MQ=255)

                                                                                 agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTCGGGGt                                                           >  1:487548/1‑149 (MQ=255)

                                                                                  gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                          <  2:439995/149‑1 (MQ=255)

                                                                                   agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGATa                                                         >  1:297357/1‑149 (MQ=255)

                                                                                    gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                        <  1:19950/149‑1 (MQ=255)

                                                                                    gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATCAGTTCAATGCGTTTACTACAAAGCATTGTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                        <  1:267435/149‑1 (MQ=255)

                                                                                     ggggatgggatAAAATGAACGTTGTAACGTTGACTTCGGATGAGTGCAATGTGTTGACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGCAGGGGTTaaa                                                       <  2:214445/148‑1 (MQ=255)

                                                                                         attggatAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAGCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  2:354004/146‑1 (MQ=255)

                                                                                         atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  1:708460/149‑1 (MQ=255)

                                                                                         atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  1:105908/149‑1 (MQ=255)

                                                                                         atgggagAAAATTAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  2:664384/149‑1 (MQ=255)

                                                                                          tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  1:519898/149‑1 (MQ=255)

                                                                                          tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  2:616026/149‑1 (MQ=255)

                                                                                          tgggatAAAATGAACCTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  1:216537/149‑1 (MQ=255)

                                                                                          gggggtAAAATTAACGTTGTAACGTTGGCTGCGGATGAGTGCAATGCGTGTACTACAAAGCATTTTTCACAGTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  2:819132/148‑1 (MQ=255)

                                                                                          ggggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  2:746873/148‑1 (MQ=255)

                                                                                              gtAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt                                              <  2:310019/148‑1 (MQ=255)

                                                                                              atAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt                                              <  1:376176/149‑1 (MQ=255)

                                                                                              atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt                                              <  2:418400/149‑1 (MQ=255)

                                                                                                   aTGGACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                         <  1:330038/149‑1 (MQ=255)

                                                                                                     gggcggtGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTCCACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                                       <  2:551724/143‑1 (MQ=255)

                                                                                                     gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                                       <  2:884003/149‑1 (MQ=255)

                                                                                                              aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTTTTTTTTTTTCAATCAGAAATCCGTTACAATCATAAAGTTGGTTTAAAAGGCGATGTGCATCTTGTATGGGTTAAAGATAACAATGGTCAAGTGACTGCAg                              >  2:678376/1‑149 (MQ=255)

                                                                                                                 gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGGAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAggct                           >  2:239111/1‑147 (MQ=255)

                                                                                                                    gaattgggATGAGTTCAATGCGTTTACTACAATGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCt                        <  2:790545/143‑1 (MQ=255)

                                                                                                                    gACTTCGGATGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCt                        <  2:660240/149‑1 (MQ=255)

                                                                                                                     aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt                       <  2:68594/149‑1 (MQ=255)

                                                                                                                          ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa                  <  1:85551/149‑1 (MQ=255)

                                                                                                                          ggATGAGTTCAATGCGTTTACTACAAAGCATGTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa                  <  1:350511/149‑1 (MQ=255)

                                                                                                                                     aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgc       <  1:796160/149‑1 (MQ=255)

                                                                                                                                         ggttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGCTTGTAGGATGCTTATTGACAGAAGCACGCacac   <  2:516877/147‑1 (MQ=255)

                                                                                                                                         gTTTACTACAAAGCATTTTTCACATTCACCACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  2:892716/149‑1 (MQ=255)

                                                                                                                                         gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  2:801298/149‑1 (MQ=255)

                                                                                                                                         gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  1:902801/149‑1 (MQ=255)

                                                                                                                                         gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  1:457735/149‑1 (MQ=255)

                                                                                                                                         ctttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  2:802169/148‑1 (MQ=255)

                                                                                                                                          tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgtactcactgt  <  2:468965/149‑4 (MQ=255)

                                                                                                                                          tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACt  <  1:711088/149‑1 (MQ=255)

                                                                                                                                          gttACTACAAAGCATTTTTCACATTACGCACAATCAGCTATTCATTTCCATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACt  <  2:190616/148‑1 (MQ=255)

TTTCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACT  >  USA300TCH1516_ALE/2532155‑2532441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`