Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	293,940	C→T	intergenic (+57/‑471)	gatD → / → tarI'	Galactitol‑1‑phosphate 5‑dehydrogenase/Ribitol‑5‑phosphate cytidylyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	293,940	0	C	T	100.0%	240.3 / NA	77	intergenic (+57/‑471)	gatD/tarI'	Galactitol‑1‑phosphate 5‑dehydrogenase/Ribitol‑5‑phosphate cytidylyltransferase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/42);  total (35/42)

TACCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAATTT  >  USA300TCH1516_ALE/293798‑294083                                                                                                                                               |                                                                                                                                                tACCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTg                                                                                                                                           >  2:477037/1‑149 (MQ=255)  aCCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTgg                                                                                                                                          >  2:844101/1‑149 (MQ=255)  aCCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTgg                                                                                                                                          <  1:365127/149‑1 (MQ=255)   ccGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGt                                                                                                                                         <  2:705286/149‑1 (MQ=255)    cGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTc                                                                                                                                        <  1:152427/149‑1 (MQ=255)     gTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCt                                                                                                                                       >  1:24275/1‑149 (MQ=255)       tAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCtat                                                                                                                                     >  1:380894/1‑149 (MQ=255)               ggCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTa                                                                                                                             <  1:446753/149‑1 (MQ=255)                   cGGAGGCATTTGTGTGATTTGTTATCATGAAAGTGTGATTTGATAAAGTCATGTTTACGCTTTATTAGTATGCGCCTTTTAGTATGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAACGTCGTCTGGTCTATAGATACTATCGa                                                                                                                         <  2:799111/149‑1 (MQ=255)                   cGGAGACATTTGATAAATTAGTTAACAAGAAAGAGCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGa                                                                                                                         <  1:531835/149‑1 (MQ=255)                   cGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGa                                                                                                                         <  1:240804/149‑1 (MQ=255)                    ggAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGaa                                                                                                                        <  1:868130/149‑1 (MQ=255)                     gagaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTGGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGaaa                                                                                                                       <  2:34814/149‑1 (MQ=255)                     aacaCATTTGATAAATAATTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGaaa                                                                                                                       <  2:490517/146‑1 (MQ=255)                         cATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAaataat                                                                                                                   <  1:580802/149‑1 (MQ=255)                          aTTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAAtt                                                                                                                  <  2:651875/149‑1 (MQ=255)                            ttGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCa                                                                                                                >  1:803727/1‑149 (MQ=255)                            ttGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCa                                                                                                                >  1:47768/1‑149 (MQ=255)                            ttGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCa                                                                                                                >  1:602135/1‑149 (MQ=255)                               aTAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCt                                                                                                             <  1:410994/149‑1 (MQ=255)                                tAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGTGTGTGCGCGGGTCTATAGATCCTCTCGAAATAATTCATCtt                                                                                                            <  2:386208/149‑1 (MQ=255)                                   aTTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGa                                                                                                         <  1:638214/149‑1 (MQ=255)                                        ttAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGCTTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCCAAATAATTCATCTTCGAatatt                                                                                                    >  2:493526/1‑148 (MQ=255)                                        ttAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAAtata                                                                                                    >  2:935104/1‑149 (MQ=255)                                         tAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGATTATAc                                                                                                   >  2:677144/1‑149 (MQ=255)                                          aaCAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACg                                                                                                  <  1:581349/149‑1 (MQ=255)                                             aagaagaaACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTg                                                                                               <  2:108120/149‑1 (MQ=255)                                             aagaaagaACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTg                                                                                               <  2:433361/149‑1 (MQ=255)                                             aagaaagaACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTg                                                                                               <  1:35413/149‑1 (MQ=255)                                                    aaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAATGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGCATTTACTTTGATCAATa                                                                                        >  2:47892/1‑149 (MQ=255)                                                        aTTTGATAAAGTCATGTTTACGATTTATTAGTATGCACTTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAAGAATTCATCTTCGAATATACGTTGATAAATAGCCg                                                                                    <  2:458140/149‑1 (MQ=255)                                                          ttGTTAAGGTCATGTTTACGTTTTATTAGTATGCCCCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGt                                                                                  <  2:306717/149‑1 (MQ=255)                                                             aTAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTa                                                                               >  1:70257/1‑149 (MQ=255)                                                                 aGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtg                                                                           <  1:382375/149‑1 (MQ=255)                                                                   tCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtg                                                                         <  1:216317/149‑1 (MQ=255)                                                                   tCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtg                                                                         <  1:512824/149‑1 (MQ=255)                                                                   tCATGTTTACGATTTATTAGTATGCACCTTGGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtg                                                                         <  1:259741/149‑1 (MQ=255)                                                                    cATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtgt                                                                        >  1:558911/1‑149 (MQ=255)                                                                    cATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtgt                                                                        >  1:158647/1‑149 (MQ=255)                                                                    cATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtgt                                                                        >  2:213510/1‑149 (MQ=255)                                                                        tttACGATTTATTAGTATGCACCTTTGACGACGAAAACGCTGGTAAAGTTATAGCTATGAAAGCGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGaaa                                                                    <  1:402987/149‑1 (MQ=255)                                                                        tttACGATTTATTAGGATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGaaa                                                                    <  1:806509/149‑1 (MQ=255)                                                                                    taGTATGCACCTTTGAGGACGAAACCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGaa                                                        >  1:646766/1‑149 (MQ=255)                                                                                    taGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGaa                                                        >  1:882321/1‑149 (MQ=255)                                                                                    taGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGaa                                                        >  1:537732/1‑149 (MQ=255)                                                                                         tGCACCTTTGAGGACGAAAACGCTGGTGTAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                                   >  1:558275/1‑149 (MQ=255)                                                                                         tGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                                   >  1:29770/1‑149 (MQ=255)                                                                                          gCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgtt                                                  >  2:509142/1‑149 (MQ=255)                                                                                           cACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGCATCGgttg                                                 >  1:867478/1‑149 (MQ=255)                                                                                           cACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttg                                                 >  2:324308/1‑149 (MQ=255)                                                                                           cACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttg                                                 >  2:442842/1‑149 (MQ=255)                                                                                           cACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGggtg                                                 >  1:667792/1‑149 (MQ=255)                                                                                               tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGtttt                                             <  1:350682/149‑1 (MQ=255)                                                                                               gttGAGCACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATCCTATCGAAATAATTCATCTTCGAATATCCGTTGGTGAATAGCCGGGTTCCTTGTGTGAAATATGCTTGTGAGTCGGTGGtttt                                             >  2:296127/2‑149 (MQ=255)                                                                                                ttGAGGACGAAAACGGTGGTATAGTTATAGCTATGAAAGTGCAAATGTCGTCGGGTCTATAGATACTATCGAAATATTTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                            <  2:418650/149‑1 (MQ=255)                                                                                                ttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                            <  2:689879/149‑1 (MQ=255)                                                                                                     gacgacAACGCTGGTATAGCTCTAGCTATGAAAGTGCGTATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAttt                                       <  1:593934/149‑1 (MQ=255)                                                                                                     gACGCAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGAAACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAttt                                       <  2:112568/149‑1 (MQ=255)                                                                                                      aCGAAACCGCTGGTATAGGTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAtttt                                      <  2:732207/149‑1 (MQ=255)                                                                                                       cGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTg                                     <  1:140945/149‑1 (MQ=255)                                                                                                        gAAAACGCTGTTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTTGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATTGGTTGTTTTGCATTTTGt                                    <  2:570056/149‑1 (MQ=255)                                                                                                           aaCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGtata                                 <  2:824653/149‑1 (MQ=255)                                                                                                             cGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACt                               <  1:697513/149‑1 (MQ=255)                                                                                                              gCTGGTATAGTAATGGCTATGAAAGGGCGAATGTCGTCTGTTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACtt                              <  1:470027/149‑1 (MQ=255)                                                                                                               cTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTa                             <  1:599455/149‑1 (MQ=255)                                                                                                                           taGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGc                 >  2:903697/1‑149 (MQ=255)                                                                                                                            aGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGGCGATCCAGTGATAAATATCCGGTTTGCTGGTGGGTTAGACTCGGAGGAGGCGGGTGGTGTGCAGTTTGTTTAATTAAAATGAGATGGCa                >  2:422820/1‑149 (MQ=255)                                                                                                                               tATGAAAGTGCGAATGTCGTCTGGTCTATAGATCATATAGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGCTGTTTTGCGTTTTGTATCCTTAAAATGAGATGGCAata             >  2:157173/1‑149 (MQ=255)                                                                                                                                 tGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATAtt           >  2:109636/1‑149 (MQ=255)                                                                                                                                  gAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATAttt          >  1:871715/1‑149 (MQ=255)                                                                                                                                  gAAAGTGCGAATGTCGTCTGGTCTATAGATAATATACAAATTATGCATCTTCGAATCTACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTCGTGCATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAatatgt          >  2:615863/1‑147 (MQ=255)                                                                                                                                   aaaGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTg         >  2:708193/1‑149 (MQ=255)                                                                                                                                        gCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAAt    >  1:90810/1‑149 (MQ=255)                                                                                                                                        gCGAATGTCGTCTGGTCAATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAAt    >  1:683705/1‑149 (MQ=255)                                                                                                                                          gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAAttt  <  2:170738/149‑1 (MQ=255)                                                                                                                                          gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAAttt  <  2:314336/149‑1 (MQ=255)                                                                                                                                          gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAAttt  <  1:447860/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                TACCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAATTT  >  USA300TCH1516_ALE/293798‑294083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑