Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	478,502	C→A	D342E (GAC→GAA)	nuoL →	NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	478,502	0	C	A	100.0%	327.8 / NA	99	D342E (GAC→GAA)	nuoL	NADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/50);  total (49/50)

TTTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTAG  >  USA300TCH1516_ALE/478356‑478647                                                                                                                                                   |                                                                                                                                                  tttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGc                                                                                                                                                 >  2:504557/1‑149 (MQ=255) tttATGTTAGTTCAATGTGCATTGGGTGTATATTAAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGc                                                                                                                                                 <  2:321167/149‑1 (MQ=255)  ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                                >  2:862011/1‑149 (MQ=255)  ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                                >  1:274019/1‑149 (MQ=255)    aTGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTa                                                                                                                                              >  1:766487/1‑149 (MQ=255)    aTGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTa                                                                                                                                              >  1:37935/1‑149 (MQ=255)     tGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                             <  1:614115/149‑1 (MQ=255)     tGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                             >  1:919789/1‑149 (MQ=255)      gTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATg                                                                                                                                            <  1:353413/149‑1 (MQ=255)      gTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCACTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATg                                                                                                                                            <  1:834580/149‑1 (MQ=255)       ttAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                           >  2:689551/1‑149 (MQ=255)        tAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGc                                                                                                                                          >  2:141109/1‑149 (MQ=255)                tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGt                                                                                                                                  <  1:335377/149‑1 (MQ=255)                tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGt                                                                                                                                  <  1:829291/149‑1 (MQ=255)                   gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCAt                                                                                                                               >  2:773782/1‑149 (MQ=255)                   gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAGTGTCAt                                                                                                                               >  2:795277/1‑149 (MQ=255)                        gggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAACCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGAc                                                                                                                          <  1:52940/149‑1 (MQ=255)                         ggTGTATATTCCGCAGCGATTATTCATTTAATATTGCACGGTATTTGTAATGCAACATTATTCTTACTATACTTTTCTATCGTGTCGTTTTTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                         <  1:445250/149‑1 (MQ=255)                         ggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                         <  1:361746/149‑1 (MQ=255)                          gtgtATATTCAGCAGCCATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                        <  2:821028/149‑1 (MQ=255)                           tgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                                       <  1:458628/149‑1 (MQ=255)                           cgtATCTCCAGCAGCGATTATTCATTCCCCATTGCCCGGTATTTTTAAAGCAACATTATTTTTCAAATCAGGTTCTATCGTGAAGCAATTCAACATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                                       <  1:654102/148‑1 (MQ=255)                            gtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtgt                                                                                                                      <  1:745493/149‑1 (MQ=255)                              atatTCAGCAGCGATTATTCATTTAATATTGCACGGTACTTTTAAAGCGACATTATCTTTACAATCAGGTTCTAGCGTGAGGCGATGCAATATTCCAAAACAAGTATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAt                                                                                                                    <  2:237083/149‑1 (MQ=255)                                 ttCCGCAGCTATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAg                                                                                                                 <  1:321837/149‑1 (MQ=255)                                 ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAg                                                                                                                 >  2:190471/1‑149 (MQ=255)                                  tctgcagcGATTATTTATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                <  2:526729/146‑1 (MQ=255)                                  tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTTCTTTCTTTTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCGTATGGCTGGCTTGTCATGGGACGTGAATTAGc                                                                                                                >  2:741973/1‑149 (MQ=255)                                  tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGGGTATTAGc                                                                                                                >  2:555187/1‑149 (MQ=255)                                  tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                >  1:923703/1‑149 (MQ=255)                                  tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                >  2:185855/1‑149 (MQ=255)                                  tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                <  2:307818/149‑1 (MQ=255)                                     gcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGTATTAGCtat                                                                                                             >  2:852156/1‑149 (MQ=255)                                      tagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGTACGTGTATTAGCtatt                                                                                                            <  2:517801/148‑1 (MQ=255)                                        gcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGAATTGTCATGGGACGTGTATTAGCtattat                                                                                                          >  2:771226/1‑149 (MQ=255)                                         ggATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTGAAGATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATCAGTTATTATc                                                                                                         <  2:110614/148‑1 (MQ=255)                                         cGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATc                                                                                                         <  1:724194/149‑1 (MQ=255)                                           attattCATTTAATATTGCACCGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGt                                                                                                       <  2:322200/149‑1 (MQ=255)                                                tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAt                                                                                                  >  2:810827/1‑149 (MQ=255)                                                 cATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTGTTATCGTGGCAtt                                                                                                 >  1:34018/1‑149 (MQ=255)                                                 cATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                                 >  1:901133/1‑149 (MQ=255)                                                   tttAATATTGCATGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                               <  1:179192/149‑1 (MQ=255)                                                   tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                               <  1:42232/149‑1 (MQ=255)                                                   tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGAAGTGTATTAGCTATTATCGTGGCATTTc                                                                                               <  2:8954/149‑1 (MQ=255)                                                    ttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                              >  1:323604/1‑149 (MQ=255)                                                      aaTATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAt                                                                                            <  2:40272/149‑1 (MQ=255)                                                      aaTATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAt                                                                                            <  2:40393/149‑1 (MQ=255)                                                       atatTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGTTTCTATCGTGAAGCGAGTCCATATTCCAAAACAAGCATCTGCTACAGAAGCTTATGGCTGAATTGTCATGGGACGTGTATTAGCTATTATCGTGGCCTTTCTAtt                                                                                           >  2:435192/1‑149 (MQ=255)                                                        tatTGCACGGTATCTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                          >  2:784024/1‑149 (MQ=255)                                                           tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTTCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTATATTAGCTATTATCGTGGCATTTTTATTTTgg                                                                                       >  2:27048/1‑149 (MQ=255)                                                           tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       >  2:25697/1‑149 (MQ=255)                                                           tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       <  1:130169/149‑1 (MQ=255)                                                           tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       <  1:392258/149‑1 (MQ=255)                                                            gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGa                                                                                      >  2:15398/1‑149 (MQ=255)                                                             cACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGGTATTATCGTGGCATTTCTATTTTGGAt                                                                                     >  1:2342/1‑149 (MQ=255)                                                                   aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagtag                                                                               <  1:810827/149‑1 (MQ=255)                                                                    tttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagtagt                                                                              >  1:872328/1‑149 (MQ=255)                                                                         aaaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAg                                                                         <  1:25697/149‑1 (MQ=255)                                                                          aaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGa                                                                        >  1:862699/1‑149 (MQ=255)                                                                          aaGCAACATTATTTTTACAATCAGGTTCGATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGCACGTGTATTAGCTATTATCGTGGCGTTTCTATTTTGGATGAGTAGTGACAGa                                                                        <  1:527706/149‑1 (MQ=255)                                                                             cAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGt                                                                     <  1:319243/149‑1 (MQ=255)                                                                              aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                                    <  1:798316/149‑1 (MQ=255)                                                                               aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                   >  1:670204/1‑149 (MQ=255)                                                                                     tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGGGTAGTGGCAGAAGTGCATATGa                                                             >  1:594853/1‑149 (MQ=255)                                                                                     tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGCCAGAAGTGCATATGa                                                             >  1:206098/1‑149 (MQ=255)                                                                                     tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCCTATGa                                                             >  1:92773/1‑149 (MQ=255)                                                                                     tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGa                                                             >  1:314066/1‑149 (MQ=255)                                                                                     tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGa                                                             >  2:549917/1‑149 (MQ=255)                                                                                        ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgt                                                          <  1:327550/149‑1 (MQ=255)                                                                                        ttACAATCAGGTTCTAGCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgt                                                          <  1:84580/149‑1 (MQ=255)                                                                                         tACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGAATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg                                                         <  1:771226/149‑1 (MQ=255)                                                                                              tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCATTTCTATTTGGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAg                                                    >  2:41166/1‑149 (MQ=255)                                                                                               cAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                                   >  1:763830/1‑149 (MQ=255)                                                                                                 ggTTCTATCGTGAAGCGATTCAATTTTCCCATACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGc                                                 <  2:652879/149‑1 (MQ=255)                                                                                                 ggTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTGTCGGGGCTTTTATCTTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGc                                                 <  1:446252/149‑1 (MQ=255)                                                                                                  gTTCTATCGTGAAGCGTTTCAATATCCCAAAACAAGGATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTAGCGTGGAATTTCTCTTTGGGATGAGAAGTGACAGAAGTGCAAATGAAGTGTTAATTGCa                                                >  2:709057/1‑149 (MQ=255)                                                                                                   ttCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAc                                               <  1:733758/149‑1 (MQ=255)                                                                                                   ttCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAc                                               <  2:801857/149‑1 (MQ=255)                                                                                                       aTCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                           >  1:295119/1‑149 (MQ=255)                                                                                                        tCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAtt                                          <  1:711884/149‑1 (MQ=255)                                                                                                        tCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGCCATTTCTATTTTGGATGAGTAGTGCTAAATGTGCATATGAAGTGTTAAGTGCACTCAtt                                          <  2:661484/149‑1 (MQ=255)                                                                                                        tCGTGAAGCGATTAAATATCCCAAAACAAGCATCTGCTGAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCTTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGCGCATATGAAGTGTTAAGTGCACTCAtt                                          <  1:605287/149‑1 (MQ=255)                                                                                                          gTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTCTTCTGGATGAGGAGTGACAGAAAGGCAGATGAAGTGTTAAGTGCACTCATTca                                        >  1:353012/1‑148 (MQ=255)                                                                                                            gAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGTTGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTTTAg                                      >  1:331997/1‑149 (MQ=255)                                                                                                               gCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGAt                                   <  1:285237/149‑1 (MQ=255)                                                                                                                 gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                                 >  1:237052/1‑149 (MQ=255)                                                                                                                 gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                                 >  1:933843/1‑149 (MQ=255)                                                                                                                 gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                                 >  1:819699/1‑149 (MQ=255)                                                                                                                   ttCAATATTTCAAAACAAGCATCTTCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTc                               <  2:683535/149‑1 (MQ=255)                                                                                                                   ttCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTc                               <  1:505473/149‑1 (MQ=255)                                                                                                                         atTCCCAACAAAGCATCTGCTTAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTTGTGTCAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACt                         <  2:899132/149‑1 (MQ=255)                                                                                                                              aaaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTACGTGCACTCACTCTAGGATGGTCATTACTTGTAt                    >  2:782253/1‑149 (MQ=255)                                                                                                                                 aCAAGCATCTGGTAAAGCAGCTTATGGCTGGATTGTCATGGGACTTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCtt                 <  2:184822/149‑1 (MQ=255)                                                                                                                                 aCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCtt                 <  1:44083/149‑1 (MQ=255)                                                                                                                                    aGCATCTGCTAAAGAAGCGTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTCTTTTGGATGAGTAGTGACAGAAGAGAATCCCATTTGCGAAGTGCACTCGTTCTAGGATGGTCATTACTTGTATCTTGGa              >  1:674006/1‑149 (MQ=255)                                                                                                                                      cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt            <  2:872328/149‑1 (MQ=255)                                                                                                                                            cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTCAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATg      >  2:253581/1‑149 (MQ=255)                                                                                                                                             tAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAACTgg     >  2:15971/1‑149 (MQ=255)                                                                                                                                                aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAGTCAGATGGTAg  >  2:503726/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TTTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTAG  >  USA300TCH1516_ALE/478356‑478647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑