Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 707,909 A→T M1M (TTG→ATG) † znuC_1 ← High‑affinity zinc uptake system ATP‑binding protein ZnuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE707,9090AT96.8% 187.7 / NA 62M1M (TTG→ATG) †znuC_1High‑affinity zinc uptake system ATP‑binding protein ZnuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (34/26);  minor base G (1/1);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGG  >  USA300TCH1516_ALE/707768‑708052
                                                                                                                                             |                                                                                                                                               
aaGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTCTTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACGTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTta                                                                                                                                          >  1:776508/1‑149 (MQ=255)
aaGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTta                                                                                                                                          >  1:29836/1‑149 (MQ=255)
aaGACTTGATAAGGGAAGATTTACCAGCACCATTAGGGCCGATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTta                                                                                                                                          >  1:925135/1‑149 (MQ=255)
  gatttGATAAGGGAAAGTTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTtata                                                                                                                                        <  1:763800/146‑1 (MQ=255)
     ttGTTAAGGGAAGATTGACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACAGGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcct                                                                                                                                     <  1:243996/149‑1 (MQ=255)
        aTAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctcct                                                                                                                                  >  1:96202/1‑149 (MQ=255)
        aTAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctcct                                                                                                                                  >  1:666842/1‑149 (MQ=255)
         tAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAACTATTTCGCCGCGTCCTGGTATCGCTCAGGAAATGTTTTTAAGTACATGCTTACTACATATAAACAGAGTTAAATCTTTTGTTTCTAACATACGTTTATCCCCCCTa                                                                                                                                 >  2:529228/1‑149 (MQ=255)
          aaGGGAAGATTTCCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGGACTGGTATCGATAAGGAATTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATCTAAAGCTTTTGTTTCTAGCATACGTTTATACCTCCTat                                                                                                                                <  2:91751/149‑2 (MQ=255)
            gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAAtt                                                                                                                              >  2:747639/1‑149 (MQ=255)
               aaGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAGCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTaaa                                                                                                                           >  1:820894/1‑149 (MQ=255)
                    ttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGttt                                                                                                                      <  1:106999/149‑1 (MQ=255)
                                        cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                                  >  2:840160/1‑149 (MQ=255)
                                           gATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAGACGTTTATACCTCCTAATTAGAAGTTTAGGCTAACCTAATTAATTGtata                                                                                               >  2:697657/1‑149 (MQ=255)
                                               ccAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTAtaataa                                                                                           <  1:332077/149‑1 (MQ=255)
                                                cAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                                          >  1:717435/1‑149 (MQ=255)
                                                cAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGAATATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                                          >  1:44613/1‑149 (MQ=255)
                                                     attTCGCGTCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgag                                                                                     <  2:81628/149‑1 (MQ=255)
                                                      tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                                                                    >  2:621298/1‑149 (MQ=255)
                                                       ttCGCCGAGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGaa                                                                                   <  1:299226/149‑1 (MQ=255)
                                                       tgctccgaGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGaa                                                                                   <  1:299115/145‑1 (MQ=255)
                                                           ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                                                               >  1:90340/1‑149 (MQ=255)
                                                           ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                                                               >  1:792040/1‑149 (MQ=255)
                                                           ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGATTCGGCTAACGTAATTAATTGTATAATAAACTGAGAATAtt                                                                               >  1:22287/1‑149 (MQ=255)
                                                              cgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                                                            >  2:275954/1‑149 (MQ=255)
                                                                   tGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAACAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGt                                                                       <  1:768072/149‑1 (MQ=255)
                                                                     gTATCGATAAGGAAATGTTTTTACGTACATGCTTATTTCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                                                                     <  1:766970/149‑1 (MQ=255)
                                                                         tgttAAGGTATTGTTTTGAACTACATGCTTATTACCTAAAAGCAGATTTAAATCTTTTTTTTCTAACATGCGTTTATACCTCCTAATGAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                                 <  2:874223/146‑1 (MQ=255)
                                                                         cGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                                 >  2:114724/1‑149 (MQ=255)
                                                                          gATAAGGAAATGTTTTTAAGTACATGCTTATTAACTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaa                                                                <  2:112406/149‑1 (MQ=255)
                                                                              aGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATTAACTGAGAATATTTATCATGTCAAGTAAATTc                                                            >  1:133769/1‑149 (MQ=255)
                                                                              aGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTc                                                            >  1:334216/1‑149 (MQ=255)
                                                                               ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                           <  2:843386/149‑1 (MQ=255)
                                                                               ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                           <  1:577173/149‑1 (MQ=255)
                                                                               ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                           <  1:534773/149‑1 (MQ=255)
                                                                                       ttttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAGATTCGTGatataa                                                   >  2:671998/1‑149 (MQ=255)
                                                                                       ttttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataa                                                   >  2:931453/1‑149 (MQ=255)
                                                                                         ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATCTTTATCATGTCAAGTCACTTCGTGttataata                                                 >  2:558556/1‑149 (MQ=255)
                                                                                                cATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGCTATAATATAGACat                                          >  2:483591/1‑148 (MQ=255)
                                                                                                cATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                          >  1:491736/1‑149 (MQ=255)
                                                                                                    cTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTATATTCGTGATATAATATAGACTatgtt                                      >  2:351829/1‑148 (MQ=255)
                                                                                                    cTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                      >  2:609240/1‑149 (MQ=255)
                                                                                                      tattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATCATAAACTGAGAATATTTATCATGTAAAGTAAATTACAGCTTCAATATAGACAttgtatg                                    >  2:890163/1‑149 (MQ=255)
                                                                                                      tattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCCACTTGATCTGTCAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatg                                    <  1:215000/149‑1 (MQ=255)
                                                                                                        ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTAtgtg                                  >  1:585030/1‑149 (MQ=255)
                                                                                                         taCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTAtgtgg                                 >  2:701632/1‑148 (MQ=255)
                                                                                                                   cAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTa                       <  2:716005/149‑1 (MQ=255)
                                                                                                                     gATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCTAGAAAATTCGTGATATAATATAGACAAAGTATGTGAGGTGAAAGTATg                     >  2:308500/1‑149 (MQ=255)
                                                                                                                      aTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGt                    <  2:29836/149‑1 (MQ=255)
                                                                                                                      aTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGt                    <  2:925135/149‑1 (MQ=255)
                                                                                                                         tAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa                 <  1:98636/149‑1 (MQ=255)
                                                                                                                             tCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga             <  1:609240/149‑1 (MQ=255)
                                                                                                                               ttttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaag           <  1:109368/149‑1 (MQ=255)
                                                                                                                                tttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaaga          >  2:528383/1‑149 (MQ=255)
                                                                                                                                    tttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaaaa      >  1:356698/1‑149 (MQ=255)
                                                                                                                                     ttCTAACATATGTTTTGACCTCCTAATCACAATGTTATGCTAACCTAACTAATGGGATAATAAACTGAGAATATTTATCATGTCAAGTAATTTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag     <  1:822460/149‑1 (MQ=255)
                                                                                                                                     ttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag     <  2:392443/149‑1 (MQ=255)
                                                                                                                                      tCTAACATCCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAaga    <  1:137995/149‑1 (MQ=255)
                                                                                                                                       cTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAagag   >  1:146546/1‑149 (MQ=255)
                                                                                                                                        tAACATACGTTTATACCTCCTAATTCAATGGCGAGGCGAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAgg  >  2:901653/1‑149 (MQ=255)
                                                                                                                                        tAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAgg  <  2:724036/149‑1 (MQ=255)
                                                                                                                                        tAACAGACGTTGATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAgg  <  2:176013/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
AAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGG  >  USA300TCH1516_ALE/707768‑708052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑