Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,314,2150CT69.9% 137.1 / 88.4 116S352N (AGT→AAT) ndhENAD(P)H‑quinone oxidoreductase subunit 4L, chloroplastic
Reads supporting (aligned to +/- strand):  ref base C (21/13);  new base T (40/39);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 117 total aligned reads displayed.

GATATACCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAACCC  >  USA300TCH1516_ALE/2314080‑2314351
                                                                                                                                       |                                                                                                                                        
gATATACCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTTTAACAATGCCAAACACATCTGCACATATACTCAtt                                                                                                                                     >  2:1347114/1‑141 (MQ=255)
gATATACCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTGTAACAATGCCAAACACATCTGCACATATACTCGtt                                                                                                                                     >  1:1036203/1‑141 (MQ=255)
gATATACCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCAtt                                                                                                                                     >  2:1251903/1‑141 (MQ=255)
gATATACCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCAtt                                                                                                                                     >  2:1310590/1‑141 (MQ=255)
    taCCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATACCAAACACATCTGCACATATATTCATTAATa                                                                                                                                 >  2:343834/1‑141 (MQ=255)
     aCCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAg                                                                                                                                <  1:205313/141‑1 (MQ=255)
      ccATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCAAATATAGTCATTAATAGa                                                                                                                               >  1:99029/1‑141 (MQ=255)
      ccATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAACAAAATTGTCCGTTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGa                                                                                                                               >  1:996283/1‑141 (MQ=255)
       cATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGAc                                                                                                                              <  1:696494/141‑1 (MQ=255)
       cATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGAc                                                                                                                              <  2:863704/141‑1 (MQ=255)
          gATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTc                                                                                                                           <  1:563249/141‑1 (MQ=255)
               cTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAAt                                                                                                                      >  1:685252/1‑141 (MQ=255)
                tttCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATg                                                                                                                     <  2:190194/141‑1 (MQ=255)
                             tAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACctct                                                                                                        <  2:1199757/141‑1 (MQ=255)
                              aaCTTCAACTTTCTATTTATAAAATAGAGTACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTa                                                                                                       >  1:865941/1‑141 (MQ=255)
                              aaCTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCCCATATATTCATTAATAGACTTCCCAATGGAATCATACatctt                                                                                                       >  1:43264/1‑140 (MQ=255)
                              aaCTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTa                                                                                                       >  1:834393/1‑141 (MQ=255)
                              aaCTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTa                                                                                                       >  2:852226/1‑141 (MQ=255)
                               aCTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTAt                                                                                                      <  1:222012/141‑1 (MQ=255)
                                     aCTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTAAAAAAGTTCTAACAATACCAAACACACCTGCACATATATTCATTAAAAGACTTCCCAATGGAGTCATACCTCTATCcatca                                                                                                >  2:256297/1‑141 (MQ=255)
                                      cTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCcatcat                                                                                               >  1:952086/1‑141 (MQ=255)
                                        ttCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCataa                                                                                             <  1:32325/141‑1 (MQ=255)
                                        ttCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCataa                                                                                             <  2:1059918/141‑1 (MQ=255)
                                        ttCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCataa                                                                                             <  2:1305563/141‑1 (MQ=255)
                                        ttCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCataa                                                                                             <  2:826267/141‑1 (MQ=255)
                                          cTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCgtaata                                                                                           >  2:392722/1‑141 (MQ=255)
                                          cTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCataata                                                                                           >  1:972773/1‑141 (MQ=255)
                                           tATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCataataa                                                                                          >  1:694473/1‑141 (MQ=255)
                                                     aTAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCACTATAATACTCAGTAc                                                                                <  2:685252/141‑1 (MQ=255)
                                                     aTAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTAc                                                                                <  1:227789/141‑1 (MQ=255)
                                                      tAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAGAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATATTAACACTCAGTACt                                                                               >  2:280813/1‑141 (MQ=255)
                                                      tAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATACTACTCAGTACt                                                                               >  2:88396/1‑141 (MQ=255)
                                                        gAATACCATTGTAATGCATATAGTACTTATTCCCATTATTTAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACttt                                                                             <  2:250282/141‑1 (MQ=255)
                                                        gAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATAGTCAGTACttt                                                                             <  2:925200/141‑1 (MQ=255)
                                                               aTTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGt                                                                      >  1:30911/1‑141 (MQ=255)
                                                               aTTGTAATGCATATAGTACTTATTCCCAATATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAGTAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGt                                                                      >  1:881956/1‑141 (MQ=255)
                                                                ttGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACTTATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGtt                                                                     >  1:1269910/1‑141 (MQ=255)
                                                                ttGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACTTCTATCCTTCATAATAATACTCAGTACTTTACCACGtt                                                                     >  1:371340/1‑141 (MQ=255)
                                                                 tGTAATGAAGATAGTAATAATTCCCATTATTAAAAAAGGTCTAACAATGCCAAACACCTCTGCCACTATTCTAATAACTAGTCTTCACAATGGAATCATCCATCTATCCATCATAATAATACTCAGTACTATAGCACGCTc                                                                    >  2:4748/1‑141 (MQ=255)
                                                                  gTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGCCTTCCCAAAGGAACCATACCTCTATCCATCATAATAATACTCACTCCTTTACCCCCTTcc                                                                   >  1:285556/1‑140 (MQ=255)
                                                                  gTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTct                                                                   >  1:428403/1‑140 (MQ=255)
                                                                        cATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCt                                                             >  1:1190526/1‑141 (MQ=255)
                                                                        cATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTAATAATCt                                                             >  2:62145/1‑141 (MQ=255)
                                                                         atatACTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCtt                                                            <  1:346623/141‑1 (MQ=255)
                                                                           ataGTACTTATTCCCAATATTGCAAAAGATCTAACAATGCCAAACACATCTGCACCAAAACCCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCCGTACTTTACCACGTTCCTAATCTTTa                                                          >  2:323403/1‑141 (MQ=255)
                                                                            taGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTaa                                                         >  1:1293206/1‑141 (MQ=255)
                                                                            taGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTaa                                                         >  1:210978/1‑141 (MQ=255)
                                                                            taGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTaa                                                         >  1:1148164/1‑141 (MQ=255)
                                                                            taGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTaa                                                         >  1:1373187/1‑141 (MQ=255)
                                                                            taGTACTTATTCCCATTATTGAAAAAGTTCTAAAAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTaa                                                         >  1:606786/1‑141 (MQ=255)
                                                                               tACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACat                                                      <  2:865941/141‑1 (MQ=255)
                                                                                 cTTATTCCCCTTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACatta                                                    <  2:1153636/141‑1 (MQ=255)
                                                                                 cTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATGTATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTGAGTACTTTACCACGTTCATAATCTTTAACatta                                                    <  2:902514/141‑1 (MQ=255)
                                                                                 cTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACatta                                                    <  2:577869/141‑1 (MQ=255)
                                                                                 cTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACatta                                                    <  2:699391/141‑1 (MQ=255)
                                                                                 cTTATTCCCAATATTGAAAAAGTTCTAAAAAGGCCAAACACCCCTGCACACAGATTCATTAATCGACTCCCCAAGGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACatta                                                    <  2:75718/141‑1 (MQ=255)
                                                                                   tATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCAGTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTGTACCACGTTCATAATCTTTAACattatt                                                  <  1:325728/141‑1 (MQ=255)
                                                                                    aTTCCCATTATTGAAAAAGTTCTAACACTGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCAATCATAATAATACTCAGTACTTTACACCGTTCATAATCTTTAACATTAttt                                                 >  2:623331/1‑141 (MQ=255)
                                                                                    aTTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTAttt                                                 >  2:521302/1‑141 (MQ=255)
                                                                                    aTTCACATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGATCATAATCTTTAACATTAttt                                                 >  2:83117/1‑141 (MQ=255)
                                                                                      tCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTg                                               <  1:81339/141‑1 (MQ=255)
                                                                                        ccATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTGTCGCTTTCTTAATCTTTAACATTATTTTGaa                                             <  1:1006268/141‑1 (MQ=255)
                                                                                        ccATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGaa                                             <  1:462632/141‑1 (MQ=255)
                                                                                        ccATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGaa                                             <  1:1063070/141‑1 (MQ=255)
                                                                                          attattGAAAAAGTTCTAACAATGCCAAACATATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGaaaa                                           >  2:440130/1‑141 (MQ=255)
                                                                                           ttattGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAAt                                          >  1:1104921/1‑141 (MQ=255)
                                                                                           ttattGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAAAATTATTTTGaaaaa                                          >  1:176453/1‑140 (MQ=255)
                                                                                                    aaGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGACTCAAGCCGCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGAt                                 <  1:1350159/141‑1 (MQ=255)
                                                                                                    aaGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGAt                                 <  1:1012976/141‑1 (MQ=255)
                                                                                                    aaGTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGAt                                 <  1:1023007/141‑1 (MQ=255)
                                                                                                      gTTCTAACAATGCCAAACCACTCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACCTTAGTTTTAAAATAAACGCGAttt                               <  1:328215/141‑1 (MQ=255)
                                                                                                      gTTCTAACAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGAttt                               <  1:1049295/141‑1 (MQ=255)
                                                                                                           aaCAATGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGt                          >  1:549703/1‑141 (MQ=255)
                                                                                                             cAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTAc                        <  2:755329/141‑1 (MQ=255)
                                                                                                             cAATGCCAAACACATCTCTATCTAGTCGCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTAc                        <  2:1377062/141‑1 (MQ=255)
                                                                                                               aTGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGt                      >  1:1078884/1‑141 (MQ=255)
                                                                                                               aTGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGt                      >  2:747976/1‑141 (MQ=255)
                                                                                                                tGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTg                     <  1:1268212/141‑1 (MQ=255)
                                                                                                                tGCCAAACACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTg                     <  1:194347/141‑1 (MQ=255)
                                                                                                                    aaaCACATCTGCACATATAAGCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACGTTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCa                 >  1:888855/1‑141 (MQ=255)
                                                                                                                     aaCACATCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTATATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTCCGTGCCCAt                >  1:116760/1‑141 (MQ=255)
                                                                                                                     aaCACATCTGCACACATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCAt                >  2:930100/1‑141 (MQ=255)
                                                                                                                       cacaTCTGCACATATATTCATTAATAGACTTCCCGATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTg              <  1:561565/141‑1 (MQ=255)
                                                                                                                       cacaTCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTg              >  2:516690/1‑141 (MQ=255)
                                                                                                                        acaTCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTTCCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGa             >  1:1350474/1‑141 (MQ=255)
                                                                                                                        acaTCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGa             >  1:643106/1‑141 (MQ=255)
                                                                                                                          aTCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATTACCGCGATTTGTCGTACGTGCCCATTGGCt           >  1:1193216/1‑141 (MQ=255)
                                                                                                                          aTCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACt           >  1:981821/1‑141 (MQ=255)
                                                                                                                          aTCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATTAACGCGATTTGTCGTATGTGCCCATTGACt           >  1:401401/1‑141 (MQ=255)
                                                                                                                          aTCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACt           >  1:546022/1‑141 (MQ=255)
                                                                                                                          aTCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACt           >  1:485447/1‑141 (MQ=255)
                                                                                                                           tCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTa          >  2:92423/1‑141 (MQ=255)
                                                                                                                           tCTGCACATATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTa          >  2:63009/1‑141 (MQ=255)
                                                                                                                               cacaTATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATa      <  2:694473/141‑1 (MQ=255)
                                                                                                                                 caTATATTCATTAATAGACTTCCCCATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAAc    <  1:280311/141‑1 (MQ=255)
                                                                                                                                 caTATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTACCAGTATTTTGAAAAGAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAAc    <  2:153745/141‑1 (MQ=255)
                                                                                                                                 caTATATTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAAc    <  1:688836/141‑1 (MQ=255)
                                                                                                                                 aatatatTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAAc    <  2:337482/140‑1 (MQ=255)
                                                                                                                                   tataGTCATTAATAGACTTCCCAATTGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAAccc  <  2:854766/141‑1 (MQ=255)
                                                                                                                                   tataCTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAAccc  <  1:440130/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GATATACCATGATTACTTTCCTCCAACTTTAACTTCAACTTTCTATTTATAAAATAGAATACCATTGTAATGCATATAGTACTTATTCCCATTATTGAAAAAGTTCTAACAATGCCAAACACATCTGCACATATACTCATTAATAGACTTCCCAATGGAATCATACCTCTATCCATCATAATAATACTCAGTACTTTACCACGTTCATAATCTTTAACATTATTTTGAAAATAAACGCGATTTGTCGTACGTGCCCATTGACTAAATAACCC  >  USA300TCH1516_ALE/2314080‑2314351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑