BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	224,684	C→T	H276Y (CAT→TAT)	USA300HOU_RS01005 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	224,684	0	C	T	97.3%	595.0 / ‑2.6	187	H276Y (CAT→TAT)	USA300HOU_RS01005	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (86/93); total (91/96)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

AAGCATAGGCATTTCACGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCACATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAATGT  >  USA300TCH1516_ALE/224546‑224817

 aGCATAGGCATTTCACGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGACCAAAtt                                                                                                                                    >  2:360719/1‑141 (MQ=255)

           tttCACGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAAcc                                                                                                                          <  1:1289646/141‑1 (MQ=255)

               aCGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACcacgg                                                                                                                      >  2:604936/1‑139 (MQ=255)

               aCGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAg                                                                                                                      >  1:937454/1‑141 (MQ=255)

               aCGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAg                                                                                                                      >  2:1356910/1‑141 (MQ=255)

                cGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACGGt                                                                                                                     >  2:746042/1‑141 (MQ=255)

                cGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGt                                                                                                                     >  2:1001812/1‑141 (MQ=255)

                  ttATGTAGGGAAGAAGTGTTTGTTACGACAGTGACTTACCGTTTCGATATGTGTCATAAAGTACGCACGGAGAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGttt                                                                                                                   <  2:1090929/141‑1 (MQ=255)

                  ttATGGATTAAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCACAAAGTACGCCCGGACAATTCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGttt                                                                                                                   <  1:69008/141‑1 (MQ=255)

                        aTTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTacgacg                                                                                                             >  1:274374/1‑141 (MQ=255)

                        aTTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTacgacg                                                                                                             <  1:1338085/141‑1 (MQ=255)

                         ttGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTacgacgc                                                                                                            <  2:109754/141‑1 (MQ=255)

                            aagaagTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTacgacgcaac                                                                                                         >  1:407284/1‑141 (MQ=255)

                               aagTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTacgacgccacgac                                                                                                      <  2:530230/141‑1 (MQ=255)

                                agggtTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCATAGTTTacgacgcaacgacg                                                                                                     <  1:724438/138‑1 (MQ=255)

                                  tgtTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTacgacgcaacgacgca                                                                                                   >  1:531887/1‑141 (MQ=255)

                                  tgtTGGATACGACAGTGACTTACCTTTTCGATCTGTGTCAGAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTcagacgcaacgccgca                                                                                                   >  1:979255/1‑141 (MQ=255)

                                   ttttGATACCACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCAt                                                                                                  <  1:919417/140‑1 (MQ=255)

                                   gtTTGATACTACAGTGACTTACCTTTCCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCAt                                                                                                  <  2:938355/141‑1 (MQ=255)

                                      tGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTgg                                                                                               <  2:1176474/141‑1 (MQ=255)

                                       gATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGt                                                                                              >  2:1196342/1‑141 (MQ=255)

                                       gATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGt                                                                                              >  1:151834/1‑141 (MQ=255)

                                         tACGACAGTGAATTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAACCGTAAAGCCCA‑ATCATTCTATATTCGATTCAACGACAGTTTACGACGCAACGACGCATTGGTTc                                                                                            <  2:966182/141‑1 (MQ=255)

                                          tccacggtgtCTTACTTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCa                                                                                           <  2:259002/135‑1 (MQ=255)

                                          aCGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCa                                                                                           <  1:795873/141‑1 (MQ=255)

                                           cGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAg                                                                                          <  2:175545/141‑1 (MQ=255)

                                             aCAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCACGACAA‑ATCTGTACATATTCGATTCAACCCAAGTTTACGCCGCCAGGCCGCATTGGTTCAGTa                                                                                        >  1:278486/1‑141 (MQ=255)

                                              cAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTaa                                                                                       >  1:360550/1‑141 (MQ=255)

                                              cAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTaa                                                                                       >  1:1357980/1‑141 (MQ=255)

                                                gTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAAcc                                                                                     <  1:229480/141‑1 (MQ=255)

                                                  gACTTGCCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGt                                                                                   <  2:80724/141‑1 (MQ=255)

                                                  gACCTACCTTTTCGATAAGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGt                                                                                   <  1:424407/141‑1 (MQ=255)

                                                   aCTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGtt                                                                                  >  1:963780/1‑141 (MQ=255)

                                                   aCTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGtt                                                                                  >  1:223906/1‑141 (MQ=255)

                                                     ttACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGa                                                                                >  2:843056/1‑141 (MQ=255)

                                                       aCCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATa                                                                              <  2:1340915/141‑1 (MQ=255)

                                                         cTTTTCGATATGTGTCATAAAGTCCGCCCGGGTAATCCGGAGGATGTAATTTGTTCGGAAACGGCGATACAA‑AGTGTTCCGTATGTTGTTCAACCACTGGTGACGACGCAGCGACGCATTGGTTCAGTAACCGTTGATAAt                                                                            <  2:1355430/141‑1 (MQ=255)

                                                         cTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACGGTTTACGCCGCAACGAAGCGTTGGTTCAGTACCCGTTGATAAt                                                                            >  2:611631/1‑141 (MQ=255)

                                                         cTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAAt                                                                            <  1:1189081/141‑1 (MQ=255)

                                                         cTTTTCGATATGTGTCATAAAGGACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGAGAA‑ATATGTCCATATTAGATTCACCCACAGTTTAAGACGCAACGACGCATTGATTCAGTAACCGTTaataat                                                                            >  2:691026/1‑141 (MQ=255)

                                                                 tatGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATag                                                                    <  2:780289/141‑2 (MQ=255)

                                                                 tatGTGTCATAAAGTACGCCCGGACAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATaa                                                                    <  2:1312243/141‑1 (MQ=255)

                                                                 aatGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATaa                                                                    <  2:239492/140‑1 (MQ=255)

                                                                    gtgtCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACgg                                                                 <  2:656204/141‑1 (MQ=255)

                                                                    gcgtCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACgg                                                                 <  1:1045213/139‑1 (MQ=255)

                                                                     tgtCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCATTAACCGTTGATAATTTGAATAACGGa                                                                >  2:387441/1‑141 (MQ=255)

                                                                     tgtCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGa                                                                >  2:443782/1‑141 (MQ=255)

                                                                      gtCATAAAGTCATCCCTGATAATCCTGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGAc                                                               <  1:637377/141‑1 (MQ=255)

                                                                      gtCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATACCGGAc                                                               <  2:14256/141‑1 (MQ=255)

                                                                      gtCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGAc                                                               <  1:1088873/141‑1 (MQ=255)

                                                                          tAAAGTACGCCCTGATAATCCTGAAAATGTAATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTa                                                           <  1:78662/141‑1 (MQ=255)

                                                                          tAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTa                                                           <  1:551346/141‑1 (MQ=255)

                                                                             aGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGACACGTCACGAGAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCAGTGGTTCAGTAACCGCTGATAATTCGAATAACGGACGTTAGCa                                                        >  2:652911/1‑141 (MQ=255)

                                                                              gTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCaa                                                       >  1:699122/1‑141 (MQ=255)

                                                                              gTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCaa                                                       >  1:388628/1‑141 (MQ=255)

                                                                               tccGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAg                                                      <  1:406419/139‑1 (MQ=255)

                                                                                aCGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAgg                                                     >  1:183373/1‑141 (MQ=255)

                                                                                 cGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGc                                                    >  1:108779/1‑141 (MQ=255)

                                                                                     cGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGgaa                                                >  2:809701/1‑141 (MQ=255)

                                                                                     cGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGaaa                                                >  1:888560/1‑141 (MQ=255)

                                                                                       gATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATg                                              <  1:351756/141‑1 (MQ=255)

                                                                                       gATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATg                                              <  1:87163/141‑1 (MQ=255)

                                                                                       gATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATg                                              <  1:1255023/141‑1 (MQ=255)

                                                                                           aTCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCaaa                                          >  1:710203/1‑141 (MQ=255)

                                                                                           aTCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTGATCAAGGCGAAATGCaaa                                          >  2:700317/1‑141 (MQ=255)

                                                                                             ccGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGGTATCAAGGCGAAATGCAAAtt                                        >  1:482193/1‑141 (MQ=255)

                                                                                                 aaaaTGTCATTCGGTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGCCGCCATCTAGGCGAAATGCAAATTGTga                                    <  1:1008647/141‑1 (MQ=255)

                                                                                                   aaTGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTgaga                                  <  1:843056/141‑1 (MQ=255)

                                                                                                    aTGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTgagaa                                 >  2:281694/1‑140 (MQ=255)

                                                                                                     tGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACa                                >  1:749894/1‑141 (MQ=255)

                                                                                                      gTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCAGATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACaa                               <  1:660977/141‑1 (MQ=255)

                                                                                                      gTCATTCGTTCGGAAACGTCAAGACAA‑ATACGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACaa                               <  2:310443/141‑1 (MQ=255)

                                                                                                       tCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACaaa                              >  1:966845/1‑141 (MQ=255)

                                                                                                       tCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACaaa                              <  2:238184/141‑1 (MQ=255)

                                                                                                       tCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACaaa                              <  1:1039253/141‑1 (MQ=255)

                                                                                                         aTTCGTTCGGAAACGTCAAGACAA‑GTATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACg                            <  2:165449/141‑1 (MQ=255)

                                                                                                         aTTCGTTCGGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACg                            <  1:1324513/141‑1 (MQ=255)

                                                                                                              ttcgGAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAg                       >  1:396207/1‑141 (MQ=255)

                                                                                                               tcgGAAACGTCAAGACAACACCTG‑CCGCATGCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGt                      <  2:278486/141‑1 (MQ=255)

                                                                                                                  gAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAGCCGTTTATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGca                   <  2:345947/141‑1 (MQ=255)

                                                                                                                  gAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGca                   <  2:85930/141‑1 (MQ=255)

                                                                                                                  gAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGca                   <  1:741430/141‑1 (MQ=255)

                                                                                                                  gAAACGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGca                   <  2:1076509/141‑1 (MQ=255)

                                                                                                                    aaCGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCCACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGcaca                 <  1:1305633/141‑1 (MQ=255)

                                                                                                                     aCGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGATGAAATGTAGCATTGGTTCAGTTCCCGTGGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGAAAAACGCTGAGTGCACAt                >  1:651891/1‑141 (MQ=255)

                                                                                                                     aCGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACACACGCTTGATGCACAt                >  1:517487/1‑141 (MQ=255)

                                                                                                                     aCGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAAGGCAAATTGTGAGACAAACGCTTAGTGCCCAt                >  1:594687/1‑141 (MQ=255)

                                                                                                                      cGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATg               >  2:415134/1‑141 (MQ=255)

                                                                                                                      cGTCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTAACAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGcatgta               >  2:337472/1‑137 (MQ=255)

                                                                                                                      cGTCAAGACAA‑ATATGTACATATTCTATTCAACAAGAGTTTTCCAAGTGACGCCGCTTTGCTTCAGTAACCGTTGATAATTTGAGTAACGGCCGAGATCAAGGTGAAAAAAAAATTGTGAGACAAACGCTTAGTGCACATg               >  2:1179494/1‑141 (MQ=255)

                                                                                                                        tCAAGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGAc             <  2:407284/141‑1 (MQ=255)

                                                                                                                           aGACAA‑ATATGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAAt          >  1:75419/1‑141 (MQ=255)

                                                                                                                                  atatGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAAt    >  2:580422/1‑141 (MQ=255)

                                                                                                                                  atatGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAAt    <  2:223906/141‑1 (MQ=255)

                                                                                                                                  atatGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGATTAGTGCAAAGGACAATGTGAAt    >  2:897692/1‑141 (MQ=255)

                                                                                                                                   aatGCCCATATTCGACTCAACCACAGTTTAAGACGCAACGACGCATTGGTTCAGTTACCGTTGCTACTGCCTGTTACTGAGGTTATAATTTGGAAGTGCAAAGTGTGAGACAAACGTTTAGTGCACATGAGGATGTGAAtg   <  1:1001812/140‑1 (MQ=255)

                                                                                                                                    atGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCCCTCTTATTAAAGGATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAAtgt  <  1:1348378/141‑1 (MQ=255)

                                                                                                                                    atGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAAtgt  <  1:305229/141‑1 (MQ=255)

                                                                                                                                    atGTCCATATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAAtgt  <  2:1048962/141‑1 (MQ=255)

                                                                                                                                    atGTCCACTTTCGATGCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAAtgt  <  2:7253/141‑1 (MQ=255)

AAGCATAGGCATTTCACGTTATGTATTGAAGAAGTGTTTGATACGACAGTGACTTACCTTTTCGATATGTGTCATAAAGTACGCCCGGATAATCCGGAAAATGTCATTCGTTCGGAAACGTCAAGACAA‑ATATGTCCACATTCGATTCAACCACAGTTTACGACGCAACGACGCATTGGTTCAGTAACCGTTGATAATTTGAATAACGGACGTTATCAAGGCGAAATGCAAATTGTGAGACAAACGCTTAGTGCACATGACAATGTGAATGT  >  USA300TCH1516_ALE/224546‑224817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`