BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	293,940	C→T	intergenic (+57/‑471)	gatD → / → tarI'	Galactitol‑1‑phosphate 5‑dehydrogenase/Ribitol‑5‑phosphate cytidylyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	293,940	0	C	T	100.0%	353.1 / NA	113	intergenic (+57/‑471)	gatD/tarI'	Galactitol‑1‑phosphate 5‑dehydrogenase/Ribitol‑5‑phosphate cytidylyltransferase 2
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (48/65); total (48/65)

Only 100 of 115 total aligned reads displayed.

TTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTT  >  USA300TCH1516_ALE/293803‑294075

ttAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGt                                                                                                                                      <  1:78642/141‑1 (MQ=255)

 tAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATgtcgtc                                                                                                                                     <  1:1275199/141‑1 (MQ=255)

 gaGAATACGGCCCGGAGACTTTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATgtcgtc                                                                                                                                     <  1:71982/140‑1 (MQ=255)

         ggCCCGGAGACATTTGATAAATTGGTTCACAAGAAAGAACGATTTGATAAAGTCATGTTTACGGTTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCtat                                                                                                                             >  2:624437/1‑141 (MQ=255)

         ggCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGGCTGGTCtat                                                                                                                             >  1:1214837/1‑141 (MQ=255)

         ggCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGGTTCAGATTGATTAGTATGCACCTTTGAAGACGAAAACGCTGGTATAGATATAGCTATGTAAGTGCGAATATCGTCTGGTTtat                                                                                                                             >  2:1055001/1‑141 (MQ=255)

          gCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCtata                                                                                                                            <  2:806654/141‑1 (MQ=255)

          gCCCGGAGACATTCGATAAATTAGTTAACAAGAAAGAACGATTTCATAAAGTCATGTTTACGAATTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGCATGTCGCCGGGTCtata                                                                                                                            >  2:639228/1‑141 (MQ=255)

             cGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGAt                                                                                                                         >  2:1072063/1‑141 (MQ=255)

             cGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGAt                                                                                                                         >  2:1341534/1‑141 (MQ=255)

               gagaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATAc                                                                                                                       >  2:91930/1‑141 (MQ=255)

                 aaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTa                                                                                                                     <  2:1250625/140‑1 (MQ=255)

                  gcATGTGATCAAGTAGTTGACGAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTAt                                                                                                                    >  2:658377/2‑141 (MQ=255)

                   cATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATc                                                                                                                   >  1:91514/1‑141 (MQ=255)

                    ttttGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCg                                                                                                                  <  1:360336/140‑1 (MQ=255)

                    aTTTGATAAATTATTTAACAAGAAAGTACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCg                                                                                                                  <  1:360390/141‑1 (MQ=255)

                       tGATAAATGTGTTAACAAGAAAGAATGATTTGATAAAGTCATGTTTACGATTTATTAGTCTGCACCTTTGAGGCCGAAAGCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGaaa                                                                                                               <  2:674470/141‑1 (MQ=255)

                         gtAAATTAGTTAACACGAAAGAACGATTTGATAAAGTCATGTTGACGATTTATTCGTATGCATCTGTGAGGACGAAAACGCTTGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAaata                                                                                                             <  2:1134462/140‑1 (MQ=255)

                            aaTTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAAtt                                                                                                          <  1:815751/141‑1 (MQ=255)

                            aaTTAGTTAACAAGAAAGAACGATTGGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGTTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAAtt                                                                                                          <  2:720915/141‑1 (MQ=255)

                            aaTTAGGGAACAAAAAAGTACCATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAAtt                                                                                                          <  2:346803/141‑1 (MQ=255)

                             aTTAGTTAACAAGACGGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTc                                                                                                         <  2:196659/141‑1 (MQ=255)

                              ttAGTTAACAAGAAAGAACCATATGAAAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCa                                                                                                        <  1:1127418/141‑1 (MQ=255)

                                 gTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCTTCTGGTCTATAGATACTATCGAAGTACTTCATCt                                                                                                     >  1:1041371/1‑141 (MQ=255)

                                    aaCAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCg                                                                                                  <  2:534782/141‑1 (MQ=255)

                                    aaCAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCg                                                                                                  <  1:71286/141‑1 (MQ=255)

                                     aCAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGa                                                                                                 <  1:123284/141‑1 (MQ=255)

                                           aagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATAc                                                                                           >  1:584146/1‑141 (MQ=255)

                                             gagcGATTTGATAAAGGCATGTTTCCGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAGTAATTCATCTTCGAATATAcct                                                                                         <  2:14618/138‑3 (MQ=255)

                                             gaaCGATTTTAGAAAGTAATGTTTACGATTTATTAGGATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGt                                                                                         <  2:1190395/141‑1 (MQ=255)

                                              aaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGtt                                                                                        >  1:1110120/1‑141 (MQ=255)

                                                     tGATAAAGTCATGTTTACAATTTAGTAGTATGCACAGTTGATGACGAATACGCTGTTATATTTATAGAGCTTAAAGTGCGTTTGTCGGCTGGTTTTTAGATACTATCGAAATAATTTATCTTCGAATATACGTTGATAAAt                                                                                 >  2:607942/1‑141 (MQ=255)

                                                       ttAAAGTTATGTTTACGATTAATAAGTCAGCTCATCTGAGCACGAAAGCGCTAGTATATTTAAAGCTACGAAATGGGGAATCTGGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAg                                                                               <  2:792353/140‑1 (MQ=255)

                                                       aTAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAg                                                                               <  2:1042584/141‑1 (MQ=255)

                                                       aTAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAg                                                                               <  2:912749/141‑1 (MQ=255)

                                                          aaGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAGTTCATCTTCGAATATACGTTGATCAATAGCCg                                                                            >  2:959234/1‑141 (MQ=255)

                                                                 ttttaataTTTATTAGTACGCACCTTTTAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACt                                                                     <  2:266965/134‑1 (MQ=255)

                                                                 ttttACGATATATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACt                                                                     <  1:810273/140‑1 (MQ=255)

                                                                 gTTTATGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACt                                                                     <  1:142128/141‑1 (MQ=255)

                                                                 gTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACt                                                                     <  2:145466/141‑1 (MQ=255)

                                                                 gTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACt                                                                     <  1:540502/141‑1 (MQ=255)

                                                                   ttACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtg                                                                   >  1:397226/1‑141 (MQ=255)

                                                                   ctACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATACACGTTGATAAATAGCCGGTTTACTtg                                                                   >  2:1020533/2‑141 (MQ=255)

                                                                          ttattaGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGaaa                                                            <  1:899732/141‑1 (MQ=255)

                                                                          ttattaGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGaaa                                                            <  1:143183/141‑1 (MQ=255)

                                                                                   tgccctcttGAGGACGAACACGATAGTATATTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTtgt                                                   <  1:1273892/134‑1 (MQ=255)

                                                                                   tGCACCTGGGAAGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTtgt                                                   <  1:1049736/141‑1 (MQ=255)

                                                                                         tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATAGTATCGACATAATTAGTCTGTGTATATGCGTTGATAGATAGCCGGTTTACTTGTGTGCAATATGCTTGTGAAtag                                             >  2:673968/1‑139 (MQ=255)

                                                                                         tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAAGATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCg                                             >  2:932162/1‑141 (MQ=255)

                                                                                         tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTAGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGGTTACTTGTGTGACATATGCTTGTGAAtgg                                             >  2:1321956/1‑139 (MQ=255)

                                                                                           tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGCAATATGCTTGTGAATCGgt                                           >  1:450485/1‑141 (MQ=255)

                                                                                           tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                           >  1:942987/1‑141 (MQ=255)

                                                                                           tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                           >  1:1077866/1‑141 (MQ=255)

                                                                                             aGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttg                                         >  2:754939/1‑141 (MQ=255)

                                                                                                aCGAAAACGGTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGTCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGttt                                      <  2:601875/141‑1 (MQ=255)

                                                                                                  gAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                    <  2:1249251/141‑1 (MQ=255)

                                                                                                  gAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                    <  2:391604/141‑1 (MQ=255)

                                                                                                  gAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                    <  2:487622/141‑1 (MQ=255)

                                                                                                  gAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCCTGGGCAACTGGTGTTTTg                                    <  2:631644/141‑1 (MQ=255)

                                                                                                  gAAAACGCTGGTATAGTTATAGCTATGAAAGGGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                    <  2:740306/141‑1 (MQ=255)

                                                                                                     aaCGCTGGTATATTTATAGCTATGAAAGTGCGAATGTCGTCGGGTCTATAGATACTATCGAAATAATTCATCTTCTAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAt                                 <  2:1056887/141‑1 (MQ=255)

                                                                                                     aaCGCTGGTATAGTTATAGCTATGAAAGTGCGACTGTCGTCTGGTCGATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAt                                 <  1:34273/141‑1 (MQ=255)

                                                                                                     aaCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATGGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAt                                 <  2:1100420/141‑1 (MQ=255)

                                                                                                     aaCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAt                                 <  1:1030772/141‑1 (MQ=255)

                                                                                                     aaCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAt                                 <  1:1145935/141‑1 (MQ=255)

                                                                                                      aCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAtt                                >  1:571788/1‑141 (MQ=255)

                                                                                                       cGCTGGTATAGTTATAGCTATGAAAGGGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAttt                               <  2:912347/141‑1 (MQ=255)

                                                                                                        gCTGGTATAGTTATAGCTATGAAAGTGCGAATGTGGTCTGGTCTATAGATACTATCGAAATAATTCATATTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATAGGTTGTTTTGCAtttt                              >  2:1254652/1‑141 (MQ=255)

                                                                                                        gCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTGTCGAAATAATTCATCTTCGAGTATACGTTGATAAATAGCCGGTTTATTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAtttt                              >  1:582277/1‑141 (MQ=255)

                                                                                                        gCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGGCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTAATTGTGTGAAATATGCTTGTGAATCGGTGGTTTTGCAtttt                              >  2:1254358/1‑141 (MQ=255)

                                                                                                         cTGGTCTAGTTAGAGCTATGAAAGGGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTg                             <  2:193513/141‑1 (MQ=255)

                                                                                                          tGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGt                            <  1:1261793/141‑1 (MQ=255)

                                                                                                           ggTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGta                           <  1:1072063/141‑1 (MQ=255)

                                                                                                            gTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGtat                          <  2:1110120/141‑1 (MQ=255)

                                                                                                              ataGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATAc                        >  1:353981/1‑141 (MQ=255)

                                                                                                              ataGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATAc                        >  1:561926/1‑141 (MQ=255)

                                                                                                              ataGTTATACCTATGAAAGTGCGAATGTCGTCCGGTCTATTGCTACTATCGAAATAATTCATCTAAACAGATACTTTGATAAATATTCTGTTTTCTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATAc                        <  1:1000705/141‑1 (MQ=255)

                                                                                                                aGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATCCTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGGTTACTTGTTTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACtt                      <  2:443152/141‑1 (MQ=255)

                                                                                                                aGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACtt                      <  1:1251613/141‑1 (MQ=255)

                                                                                                                     taGATAGGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAAt                 <  2:975706/141‑1 (MQ=255)

                                                                                                                     aatcTATGAATGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGGGAATCGGTTGTTTTGCATTTTGTATACTTAAAAt                 <  2:251404/138‑1 (MQ=255)

                                                                                                                       gCTCTGAAAGTGCGCATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATga               <  1:114778/141‑1 (MQ=255)

                                                                                                                       gCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATga               <  1:688671/141‑1 (MQ=255)

                                                                                                                       gCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATga               <  1:721523/141‑1 (MQ=255)

                                                                                                                       gCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATga               <  1:113009/141‑1 (MQ=255)

                                                                                                                        cTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATgag              >  1:1146505/1‑141 (MQ=255)

                                                                                                                        cTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATgag              >  2:389633/1‑141 (MQ=255)

                                                                                                                         tataAAAGTGCGTAGGTCGGCGGGTCTAGAGATACTCTCGAAATAATTCATCTTCGAGTATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCACGTGGATGGGAGGTTTTGCATTTTGTATACTTAAAATgaga             >  2:282389/1‑141 (MQ=255)

                                                                                                                          aTGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGAt            <  1:476946/141‑1 (MQ=255)

                                                                                                                           tGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATg           >  1:962132/1‑141 (MQ=255)

                                                                                                                           tGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATg           >  2:431104/1‑141 (MQ=255)

                                                                                                                           tGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATg           >  2:1105746/1‑141 (MQ=255)

                                                                                                                            ggaaGTGCGAATGTCGTCTGGTTTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATgg          <  2:327341/139‑1 (MQ=255)

                                                                                                                            gAAAGTGCGGTTGTCGTCTGGTCTATAGGTACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAAGGAGATgg          <  2:1266967/141‑1 (MQ=255)

                                                                                                                            aaaaGTGCTAATGTCGTCTGGTCTATAGATACTAGTGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATgg          <  2:661048/140‑1 (MQ=255)

                                                                                                                               aGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATGTACGTTGCTAAATGGCCGGTTTAATTGTGTGAAATATGCATGTGAATCAGATCGTTTACATGTTGTATACTAAAAATGAGAAGGaaa       >  1:1067919/1‑141 (MQ=255)

                                                                                                                                  gCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCTatgt    >  2:248873/1‑139 (MQ=255)

                                                                                                                                  gCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTGTGTATACTTGAAATGAGATGGCAatat    >  1:1126885/1‑141 (MQ=255)

                                                                                                                                   cGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAGTAtt   >  2:34311/1‑141 (MQ=255)

                                                                                                                                    gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATAttt  <  1:665245/141‑1 (MQ=255)

TTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTT  >  USA300TCH1516_ALE/293803‑294075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`