BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,455,698	2 bp→AT	coding (1216‑1217/1260 nt)	lyrA →	Lysostaphin resistance protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,455,698	0	C	A	93.7%	355.6 / 15.3	116	coding (1216/1260 nt)	lyrA	Lysostaphin resistance protein A
Reads supporting (aligned to +/- strand): ref base C (1/2); major base A (43/61); minor base . (3/4); total (49/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	USA300TCH1516_ALE	2,455,699	0	A	T	94.8%	346.9 / ‑4.8	118	H406L (CAT→CTT)	lyrA	Lysostaphin resistance protein A
Reads supporting (aligned to +/- strand): ref base A (2/4); new base T (46/64); total (49/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACA‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTTGA  >  USA300TCH1516_ALE/2455566‑2455838

||

tCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGaaa                                                                                                                                     <  2:651004/141‑1 (MQ=255)

 cAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAATACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGaaaa                                                                                                                                    <  1:1356534/141‑1 (MQ=255)

 cAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGaaaa                                                                                                                                    <  2:315742/141‑1 (MQ=255)

   attAAAAAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGaaaaaa                                                                                                                                  <  2:234397/140‑1 (MQ=255)

        ataaGCACGACAAATTGACGATTTAACAAACGATACAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTgaa                                                                                                                             <  2:1211616/139‑1 (MQ=255)

        aCAAGCACCACAAAGTGACGATAAAACAAAAGATACAAATGAAGAAGAAATAGAGCAATCATAAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAA‑AAAAAAAAAAACTgaa                                                                                                                             <  2:1211224/141‑1 (MQ=255)

            gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATGGAAAAAACTGAAGAAc                                                                                                                         <  1:812954/141‑1 (MQ=255)

            gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAAc                                                                                                                         <  1:1090393/141‑1 (MQ=255)

            gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAAc                                                                                                                         <  1:599506/141‑1 (MQ=255)

            gCACCACAAAGTGACGATTGAACAAACGATTCAAATGAAGATGAAAGAGAGCAATCATTAAAAGAACCTGCCACTTATAAAGAAGACAGACGTTTATCAGTTGAAAAAAAAAAA‑AAAAAAT‑TATCGAAAAAACTGAAGAAc                                                                                                                         <  2:344450/141‑1 (MQ=255)

            gCACCACAAAGTCACGATTTAACAAACGATTCAAATAAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAAc                                                                                                                         <  1:1222960/141‑1 (MQ=255)

            gCACCAAAAAGTGACGATTTAAACAACGATTCAAAGGAAGATGAAATAGAGCAATTATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGCTCAACAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAAc                                                                                                                         <  2:557322/141‑1 (MQ=255)

            acaccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGACCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAAc                                                                                                                         <  1:788518/140‑1 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAGGAACCTGCGACTTATAAAGAAGACTGACGTTCATCAGTTGTAATTGATGC‑TAAAAAAT‑GATCGAAAAAACTGAAGAACa                                                                                                                        <  1:885218/141‑1 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑T‑TATCGAAAAAACTGAAGAACa                                                                                                                        >  2:52309/1‑141 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAGT‑TATCGAAGAAACTGAAGAACa                                                                                                                        >  2:366941/1‑141 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACa                                                                                                                        >  2:458535/1‑141 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACa                                                                                                                        >  2:1073865/1‑141 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACa                                                                                                                        <  1:441452/141‑1 (MQ=255)

             caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCAGTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACa                                                                                                                        <  1:857266/141‑1 (MQ=255)

               ccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAAt                                                                                                                      <  2:134686/141‑1 (MQ=255)

                 acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGTA‑ACACACG‑TATCGAACAACCTCACTAACAATCt                                                                                                                    >  2:672992/1‑141 (MQ=255)

                  caAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCCTCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAAATGAAGAACAATCtt                                                                                                                   >  2:778992/1‑141 (MQ=255)

                  caAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCtt                                                                                                                   >  2:1133563/1‑141 (MQ=255)

                         aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAACAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAGCTGAAGAACAATCTTCAGATaa                                                                                                            <  1:1286260/141‑1 (MQ=255)

                         aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATaa                                                                                                            >  2:145250/1‑141 (MQ=255)

                         aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATaa                                                                                                            <  1:679047/141‑1 (MQ=255)

                         aCGATTTAACAAACGATTCAAATGAAGAGGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATaa                                                                                                            <  1:179706/141‑1 (MQ=255)

                           gATTTAACAAACGATTCAAATGAAGATGAACTAGAGCAATCATTCAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCCGATaaaa                                                                                                          >  1:809292/1‑141 (MQ=255)

                           gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                                          <  1:200312/141‑1 (MQ=255)

                             tttAACAAAGGATTCAAAGGAAGATGAAACAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc                                                                                                        <  2:1366861/141‑1 (MQ=255)

                             tttAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc                                                                                                        <  1:998307/141‑1 (MQ=255)

                             tttAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATAAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc                                                                                                        <  2:617044/141‑1 (MQ=255)

                                 aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat                                                                                                    <  1:496370/141‑1 (MQ=255)

                                 aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat                                                                                                    <  1:410919/141‑1 (MQ=255)

                                  cAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGTA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaata                                                                                                   >  2:72514/1‑141 (MQ=255)

                                  cAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATA‑‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAACCAaata                                                                                                   >  2:1118359/1‑141 (MQ=255)

                                  cAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaata                                                                                                   >  2:693544/1‑141 (MQ=255)

                                  cAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaata                                                                                                   >  2:1199752/1‑141 (MQ=255)

                                  cAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaata                                                                                                   >  1:1170761/1‑141 (MQ=255)

                                    aaCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaataaa                                                                                                 >  2:1195531/1‑140 (MQ=255)

                                         ttCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                            >  2:953081/1‑141 (MQ=255)

                                         atCAAAAGAAGATAAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAATGATGCA‑AAAAAAA‑TATCGAAAAAACTGAAGAACAATCTTCAAAAAAACACAAATAATAACaa                                                                                            <  2:905410/140‑1 (MQ=255)

                                           cAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAGAATGAA‑TA‑CGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaaa                                                                                          >  1:1062995/1‑140 (MQ=255)

                                           cAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                                          >  1:1255052/1‑141 (MQ=255)

                                                  aGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGGTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGc                                                                                   <  1:857109/141‑1 (MQ=255)

                                                    aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAA‑T‑TATCGAAAAAACTGAAGAATAATCTTCACATAAAAACATATAATAACAAAGCGCCTGCTa                                                                                 <  1:1008611/141‑1 (MQ=255)

                                                    aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTAAAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTa                                                                                 <  2:135478/141‑1 (MQ=255)

                                                      gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                               >  1:151979/1‑141 (MQ=255)

                                                       aaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGAGCTTACTAGtc                                                                              >  2:28696/1‑140 (MQ=255)

                                                       aaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAAAAAAAATAACAAAGCGATTGctacta                                                                              >  2:814756/1‑141 (MQ=255)

                                                            gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCCGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                                         <  1:256835/141‑1 (MQ=255)

                                                                    aTTAAAGGAAAATGCGACTTATAAAGAAGACAGACGTTCACCAGTAGTAATTGACGC‑CAAAAAAT‑TATCGAAAAAACTGAAGAACAACCTTCAGATAAAAACAAATATAAACCAAGAGCTAACTAGTACCCCTCAACAAga                                                                 >  2:868857/1‑141 (MQ=255)

                                                                    aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAAAAATAATAACAAAGCGCTAGCTAGTCCCTCTTaaaaaaa                                                                 >  2:4121/1‑139 (MQ=255)

                                                                     ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTACATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTAAAAAgat                                                                >  1:948306/1‑141 (MQ=255)

                                                                       aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAcatga                                                              >  1:1300925/1‑141 (MQ=255)

                                                                       aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTAAAAAgatga                                                              >  1:1277464/1‑141 (MQ=255)

                                                                         aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgctg                                                            >  2:503136/1‑141 (MQ=255)

                                                                          aGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGc                                                           <  1:806964/141‑1 (MQ=255)

                                                                             aCCTGCGTCTTATAAAGAAGACAGATGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                        <  2:430335/141‑1 (MQ=255)

                                                                             aCCGGCGACTTATAAAGAAGACAGACGTTCATAAGTTGTAATTTATGCAAAAAAAC‑‑TATCGAAAAATCTGAAGAAAAAAAAAAAAAAAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                        <  2:1283360/141‑1 (MQ=255)

                                                                              ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑T‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc                                                       >  1:776914/1‑141 (MQ=255)

                                                                               cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                                      <  1:610406/141‑1 (MQ=255)

                                                                                tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                                     >  1:1210487/1‑141 (MQ=255)

                                                                                tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                                     >  1:444465/1‑141 (MQ=255)

                                                                                tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACCGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTACCaa                                                     >  1:668536/1‑141 (MQ=255)

                                                                                tGCGACTTATAAAGAAGACAGACGTTCATCAGTAGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                                     <  2:1050882/141‑1 (MQ=255)

                                                                                  cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                                   >  1:743731/1‑141 (MQ=255)

                                                                                  cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                                   <  2:1262709/141‑1 (MQ=255)

                                                                                  cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTAAAAAAGATGATGCTAGCAAgc                                                   <  2:1180562/141‑1 (MQ=255)

                                                                                   gACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAACAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg                                                  <  1:120967/141‑1 (MQ=255)

                                                                                    aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTCCCACTTAAAACGATGATGCTAGCAAgcgc                                                 >  1:1190371/1‑141 (MQ=255)

                                                                                    aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgccc                                                 >  2:1298692/1‑139 (MQ=255)

                                                                                     cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑T‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                                >  1:842519/1‑141 (MQ=255)

                                                                                     cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                                <  2:1290815/141‑1 (MQ=255)

                                                                                     cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAGATGCTGCTAGCAAgcggt                                                >  2:330207/1‑139 (MQ=255)

                                                                                      ttATAAACAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑T‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtt                                               <  1:23827/141‑1 (MQ=255)

                                                                                        ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCACGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                             <  1:662284/141‑1 (MQ=255)

                                                                                         taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                                            <  1:1168335/141‑1 (MQ=255)

                                                                                               agacagacGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatact                                      >  1:1110375/1‑141 (MQ=255)

                                                                                                 acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAACCTGAAGAACAATCTTCAGATAAAAACAAATAATAACCATGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                                    <  2:1112098/141‑1 (MQ=255)

                                                                                                 acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                                    >  2:887631/1‑141 (MQ=255)

                                                                                                  cagacGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                                   >  1:937424/1‑141 (MQ=255)

                                                                                                  cagacGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                                   >  1:1219547/1‑141 (MQ=255)

                                                                                                     acGTTCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTatatat                                <  1:715973/141‑1 (MQ=255)

                                                                                                       gTTCATCAGTTGTAATTGATGCAAAAAAA‑T‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatta                              <  1:999983/141‑1 (MQ=255)

                                                                                                        ttCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                             <  2:769257/141‑1 (MQ=255)

                                                                                                        ttCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                             <  2:788391/141‑1 (MQ=255)

                                                                                                        ttCATCAGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                             <  2:1366341/141‑1 (MQ=255)

                                                                                                        ttCATCAGTTGTAATTGAATCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTATTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                             <  2:655438/141‑1 (MQ=255)

                                                                                                        ttCAGCAGGTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                             <  2:534774/141‑1 (MQ=255)

                                                                                                            tcaGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt                         <  1:19161/141‑1 (MQ=255)

                                                                                                             caGTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTGCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTACACTATATATTATTTTTc                        <  2:567269/141‑1 (MQ=255)

                                                                                                               gTTGTAATTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTa                      <  1:193825/141‑1 (MQ=255)

                                                                                                               gTTGTAAATGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTa                      <  2:1249657/141‑1 (MQ=255)

                                                                                                                     aTTGATGCA‑AAAAAAT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTATTCTATACTATATATTATTTTTCTAAACttt                >  1:1259267/1‑141 (MQ=255)

                                                                                                                         tatgcaaaaaaaT‑TATCGAAAAAACTGAAGAACAATCTGCTCTTAATAACAACTCGTCGCTAGGCCGTTGCTCTTACCTCGTAATTGGATAAGGCTATCAAACGCTATTCTATACTATATATTATTTTTCTAAAATTTTaa             <  2:653929/135‑1 (MQ=255)

                                                                                                                          atgcaaaaaaaT‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAac            <  2:195201/136‑1 (MQ=255)

                                                                                                                            ggcaaaaaCATTATCGACACAACTGAAGAACAATCTTCAGATAAAAGCAAATAATAACACAGCGCTTGCTAGTACCTCCTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca           <  2:739410/138‑1 (MQ=255)

                                                                                                                                    aa‑taaCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTTGa  <  2:133602/140‑1 (MQ=255)

||

TCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACA‑TATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTTGA  >  USA300TCH1516_ALE/2455566‑2455838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`