| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 19,009 | C→T | 100% | S222F (TCC→TTC) | gdpP → | Cyclic‑di‑AMP phosphodiesterase GdpP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 19,009 | 0 | C | T | 100.0% | 26.8 / NA | 9 | S222F (TCC→TTC) | gdpP | Cyclic‑di‑AMP phosphodiesterase GdpP |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (4/5); total (4/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTCCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGGTGTTGG > USA300TCH1516_ALE/18906‑19141 | cAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTa > 2:23014/1‑141 (MQ=255) aatgaTACACAGCATTAGGAAATCAACTCAATGGTAACGCGTGTCATTAGTGGATGGGCAACTGAGTATAATATATTATTCAAAAGATACAGTTTCGATCAATTCGTAGACAAAAAAAAAAAAAAAATATTAGCTGACTTa < 2:7421/141‑1 (MQ=255) tCGGAAATCAACTAAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTATCAAAAGATACAGTTTCGATCAATTCGTAGCCAAAAAAAAAAAAAAAATATTAGCTGACTTAGAAGAATCTAAAttt < 2:14527/141‑1 (MQ=255) tCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTgg > 2:52175/1‑141 (MQ=255) gATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGtt < 1:19834/141‑1 (MQ=255) gCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGt > 1:53721/1‑141 (MQ=255) tGAGAATAATATATTTTTCAAAAGATACGGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGCGTCAATTAAGAGAAAAAAGTGTGTGTTATCGTGCCCa > 2:6101/1‑141 (MQ=255) aaaGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGgtg < 1:62287/141‑1 (MQ=255) aTACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGGTGTTgg < 1:17706/141‑1 (MQ=255) | CAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTCCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGGTGTTGG > USA300TCH1516_ALE/18906‑19141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |