| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,455,692 | Δ1 bp | 100% | coding (1210/1260 nt) | lyrA → | Lysostaphin resistance protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,455,692 | 0 | G | . | 93.5% | 586.8 / 23.5 | 138 | coding (1210/1260 nt) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base G (1/1); major base . (78/51); minor base A (5/2); total (84/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
Only 100 of 145 total aligned reads displayed.
AGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACAT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT > USA300TCH1516_ALE/2455559‑2455825 | agcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatt > 3:300412/1‑139 (MQ=255)agcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatt > 4:16317/1‑139 (MQ=255) gcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta > 1:187128/1‑138 (MQ=255) agAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatc < 4:491047/141‑6 (MQ=255) aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGaaaaa > 2:236046/1‑141 (MQ=255) aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGaaaaa > 4:302098/1‑141 (MQ=255) gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGaaaaaa > 2:1123513/1‑141 (MQ=255) gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGaaaaaa > 4:292180/1‑141 (MQ=255) gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGaaaaaa > 2:808881/1‑141 (MQ=255) ttcaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAAc < 4:433680/138‑1 (MQ=255) ttAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TT‑ATCGAAAAAAc > 1:49784/1‑141 (MQ=255) cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTCAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTgaag < 2:628473/141‑1 (MQ=255) cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCGTCAGTTGTGGTTGCTGC‑‑AACAACATAATCGAAAAAACTgaag > 3:18171/1‑141 (MQ=255) cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTgaag > 1:588477/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAAc > 1:35485/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAAc > 2:341813/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAAc > 1:720232/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAAc > 3:335463/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAAAAAAAAA‑AAAAAATT‑ATCGAAAAAACTGAAGAAc < 2:1088280/141‑1 (MQ=255) cacaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATc > 1:637707/1‑141 (MQ=255) acaAAGTGACGATTTAACAAACGATTAAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGAAAAAAAAAAA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCt < 2:119089/141‑1 (MQ=255) aaaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTc > 1:408928/1‑141 (MQ=255) aaatGACGATTAAACAAACGATTCAAATGAAGAAGAAAAAAAGGAATAAATAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAAA‑AAAAAAAAAACTGAAGAACAATCTTCa < 2:488294/138‑1 (MQ=255) aaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCa > 2:156324/1‑141 (MQ=255) aaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCa < 1:737421/141‑1 (MQ=255) tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGAt < 2:793185/141‑1 (MQ=255) tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGAt < 2:48620/141‑1 (MQ=255) gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATa > 1:1174237/1‑141 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATaaaa < 2:704794/141‑1 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATaaaa < 3:14579/141‑1 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATaaaa > 2:706197/1‑141 (MQ=255) aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat < 3:293859/141‑1 (MQ=255) cGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAtaataa < 1:1164968/141‑1 (MQ=255) cGATTCAAATGAAGATGAAAGATAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAtaataa < 3:128921/141‑1 (MQ=255) gtgtaaaTGAAGATGAAATAGAGCAATCAATAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa > 2:70727/5‑141 (MQ=255) aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa < 1:671919/141‑1 (MQ=255) ttCAAATGAAGATGAAATAGAGCAATCATTACAAGATCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa < 1:231677/141‑1 (MQ=255) aacAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCAACTTAAAAAAAAAACAGACGTAAAAAAGAAGAAAAAAAAAAA‑AAAAAAAA‑ATCAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa < 2:1055614/139‑1 (MQ=255) aaaaTGAAGAGGAAAAAGAGAAATCATTAAAAGAGCCTGCGACTAATAAAGAAGACAGACGTTCATCAGTTGCAATTGATACA‑AAAAAAAT‑ATCGAAAAAACTGAAGAACAAAAAAAAGATAAAAACAAATAATAACAAAg < 2:19585/140‑1 (MQ=255) aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc < 1:342976/141‑1 (MQ=255) aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAga > 1:296013/1‑140 (MQ=255) aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg > 2:819624/1‑141 (MQ=255) aTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAA‑TT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCt < 2:819402/141‑1 (MQ=255) tGAAGATGAAATAGAGCAGTCGTTAAAAGAACATGAGACTTATAAAGAAGAAAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCt < 2:514606/141‑1 (MQ=255) gAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCtt < 4:473650/141‑1 (MQ=255) aGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGc > 2:362381/1‑141 (MQ=255) tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg > 2:221209/1‑141 (MQ=255) gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt > 2:954765/1‑141 (MQ=255) aTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc > 2:719799/1‑141 (MQ=255) aTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc > 3:217095/1‑141 (MQ=255) tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct > 1:1015434/1‑141 (MQ=255) agagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctc < 3:322172/141‑1 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct < 1:341813/141‑1 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct > 3:462732/1‑141 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct < 3:16317/141‑1 (MQ=255) gCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACAT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt < 1:310965/141‑1 (MQ=255) cAATCATTAAAAGAACCTGCGACTTATAAAGCAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTaa > 1:820445/1‑141 (MQ=255) aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTaaa > 1:14602/1‑141 (MQ=255) aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa > 2:447700/1‑141 (MQ=255) aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga > 2:429351/1‑141 (MQ=255) aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatgat > 1:1112093/1‑141 (MQ=255) aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAgatgat > 4:112457/1‑141 (MQ=255) aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAgatgat > 1:169560/1‑141 (MQ=255) aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCACTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatgat < 1:881786/141‑1 (MQ=255) aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg > 1:851288/1‑141 (MQ=255) aGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAATGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGc < 4:52016/141‑1 (MQ=255) gAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt > 1:530122/1‑141 (MQ=255) aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCCTGCTAGTACCTCTTAAAAAGATGATGCTAg > 1:881732/1‑141 (MQ=255) aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg > 2:717987/1‑141 (MQ=255) aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg > 2:563020/1‑141 (MQ=255) aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg > 2:100957/1‑141 (MQ=255) cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 1:164585/141‑1 (MQ=255) gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg > 4:291687/1‑141 (MQ=255) gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg > 4:26370/1‑141 (MQ=255) cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTgaaga < 3:81727/69‑1 (MQ=255) cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTgaaga > 4:81727/1‑69 (MQ=255) gACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg > 1:851229/1‑141 (MQ=255) acatataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc < 4:328811/138‑1 (MQ=255) cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt > 2:825966/1‑141 (MQ=255) ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACaa > 1:727388/1‑84 (MQ=255) ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACaa < 2:727388/84‑1 (MQ=255) taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttta < 1:1133175/141‑2 (MQ=255) taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc > 1:74850/1‑141 (MQ=255) aaaGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAGAAATC‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTct > 1:1166883/1‑141 (MQ=255) aagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac < 2:1035951/141‑1 (MQ=255) acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat > 3:493820/1‑141 (MQ=255) acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat > 1:553979/1‑141 (MQ=255) agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat < 1:194185/141‑1 (MQ=255) cGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt > 2:951202/1‑141 (MQ=255) cGTTCATCAGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt < 2:735936/141‑1 (MQ=255) catcaGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttt < 1:1071061/141‑1 (MQ=255) tcaGTTGTAATTGATGCAAAAAAA‑TT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt < 2:290903/141‑1 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt > 1:60700/1‑141 (MQ=255) caGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAGACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc < 1:445328/141‑1 (MQ=255) caGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc < 1:445723/141‑1 (MQ=255) caGTTGTAATTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc < 4:439987/141‑1 (MQ=255) aTTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt > 4:156624/1‑141 (MQ=255) aTTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt > 2:1173377/1‑141 (MQ=255) aTTGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt > 2:6421/1‑141 (MQ=255) ttGATGCA‑AAAAAATT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt < 1:1123513/141‑1 (MQ=255) | AGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACAT‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT > USA300TCH1516_ALE/2455559‑2455825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |