BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	119,853	C→A	51.8%	G254W (GGG→TGG)	USA300HOU_RS00550 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	119,853	0	C	A	51.8%	30.9 / inf	184	G254W (GGG→TGG)	USA300HOU_RS00550	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (40/48); new base A (49/46); total (89/95)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 186 total aligned reads displayed.

TTGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAATTAAA  >  USA300TCH1516_ALE/119716‑119992

ttGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGAt                                                                                                                                          <  2:1065346/141‑1 (MQ=255)

ttGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGAt                                                                                                                                          <  1:371803/141‑1 (MQ=255)

ttGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACGAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGac                                                                                                                                          <  3:385415/141‑2 (MQ=255)

 tGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGAtt                                                                                                                                         >  3:10340/1‑141 (MQ=255)

      cAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAAACAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGtt                                                                                                                                    <  1:1013582/141‑1 (MQ=255)

        aaGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTaa                                                                                                                                  <  2:592691/141‑1 (MQ=255)

         aGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaaa                                                                                                                                 <  1:627206/141‑1 (MQ=255)

         aGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaaa                                                                                                                                 >  1:507376/1‑141 (MQ=255)

         aGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaaa                                                                                                                                 <  1:405877/141‑1 (MQ=255)

            aTTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGAt                                                                                                                              <  2:458371/141‑1 (MQ=255)

              ttGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCCAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGa                                                                                                                            >  3:490225/1‑141 (MQ=255)

              ttGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGa                                                                                                                            >  2:83613/1‑141 (MQ=255)

                 ttATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACgt                                                                                                                         >  4:324873/1‑141 (MQ=255)

                     gTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGtt                                                                                                                                    <  1:384887/126‑1 (MQ=255)

                       tGAAGATACCATGTGATCCTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAACCCCAACTGGCAATAAAATTGTGAAGATAATTACTCCGATTAAGTTAAAGATGACGTGTACaa                                                                                                                   <  4:364758/141‑1 (MQ=255)

                        gAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAg                                                                                                                  <  2:339817/141‑1 (MQ=255)

                          aGATAACATGTGATACTGCAATAGTCATCGATGGTAGTAAGTGACATAAATCTTGTAAGAAAATAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACACAGATTAAGTTAAAGATGACGTGTACAAgcg                                                                                                                >  4:269318/1‑141 (MQ=255)

                           caTACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAgcgc                                                                                                               <  2:687465/140‑1 (MQ=255)

                             tACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGcgccg                                                                                                             >  1:1109125/1‑141 (MQ=255)

                               ccATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCa                                                                                                           <  1:1151889/141‑1 (MQ=255)

                                 aTGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACg                                                                                                         >  2:768117/1‑141 (MQ=255)

                                    tgATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGttt                                                                                                      <  2:187969/141‑1 (MQ=255)

                                    tgATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGttt                                                                                                      <  4:489428/141‑1 (MQ=255)

                                         cTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAg                                                                                                 >  1:47998/1‑141 (MQ=255)

                                          tGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGc                                                                                                <  1:680804/141‑1 (MQ=255)

                                              aTCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGAtt                                                                                            >  4:190894/1‑141 (MQ=255)

                                                 gTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAg                                                                                         >  2:697569/1‑141 (MQ=255)

                                                 gTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAg                                                                                         <  4:406297/141‑1 (MQ=255)

                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                                       >  1:329215/1‑141 (MQ=255)

                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                                       <  1:244287/141‑1 (MQ=255)

                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                                       >  3:103509/1‑141 (MQ=255)

                                                     tCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCgg                                                                                     >  4:67102/1‑141 (MQ=255)

                                                           gTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAAc                                                                               <  3:339427/141‑1 (MQ=255)

                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                                            <  3:67102/141‑1 (MQ=255)

                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                                            >  3:176467/1‑141 (MQ=255)

                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                                            >  1:602043/1‑141 (MQ=255)

                                                                aaGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACTAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCt                                                                          >  2:411818/1‑141 (MQ=255)

                                                                aaGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCt                                                                          <  2:1109125/141‑1 (MQ=255)

                                                                 aGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTa                                                                         >  3:99241/1‑141 (MQ=255)

                                                                 aGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTa                                                                         >  1:933263/1‑141 (MQ=255)

                                                                  gTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTaa                                                                        >  1:925498/1‑141 (MQ=255)

                                                                    gCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGa                                                                      >  2:558059/1‑141 (MQ=255)

                                                                    gCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTCAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAgg                                                                      >  2:557892/1‑140 (MQ=255)

                                                                    gCCATAAATCTTATAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGa                                                                      <  2:829764/141‑1 (MQ=255)

                                                                      cATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATa                                                                    >  4:46871/1‑141 (MQ=255)

                                                                         aaaTCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCt                                                                 <  2:1149561/141‑1 (MQ=255)

                                                                          aaTCTTGTAACAGACTAATCAAATGACTCACAACGGGCAAGAAAATTGTGACGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTg                                                                >  4:8466/1‑141 (MQ=255)

                                                                          aaTCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGACAATTACCCCGATTAAGTTAAAGATGACTTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTg                                                                <  2:949897/141‑1 (MQ=255)

                                                                            tCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTa                                                              >  3:377839/1‑141 (MQ=255)

                                                                             cTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTaa                                                             <  1:1103402/141‑1 (MQ=255)

                                                                               tGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGGTAAAGAGGACATGTATAATCGCCGCACGTTTTGCAGCGAGTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATc                                                           >  1:596684/1‑141 (MQ=255)

                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCg                                                          >  3:462740/1‑141 (MQ=255)

                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGCTGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCg                                                          >  3:253100/1‑141 (MQ=255)

                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAg                                                                                                 >  2:451453/1‑102 (MQ=255)

                                                                                 tAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGt                                                         <  2:628562/141‑1 (MQ=255)

                                                                                  aaCAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTg                                                        <  1:253486/141‑1 (MQ=255)

                                                                                         tAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCaa                                                 >  1:166408/1‑141 (MQ=255)

                                                                                         tAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCaa                                                 <  3:203222/141‑1 (MQ=255)

                                                                                            tCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGt                                              <  1:749988/141‑1 (MQ=255)

                                                                                            tCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGt                                              <  4:315786/141‑1 (MQ=255)

                                                                                            tCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGt                                              <  2:1153375/141‑1 (MQ=255)

                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                                            <  3:392571/141‑1 (MQ=255)

                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                                            <  1:1186339/141‑1 (MQ=255)

                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                                            >  4:389311/1‑141 (MQ=255)

                                                                                                aTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATc                                          >  2:37454/1‑141 (MQ=255)

                                                                                                    aTCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCt                                      >  2:92182/1‑141 (MQ=255)

                                                                                                     tCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTa                                     >  2:16643/1‑141 (MQ=255)

                                                                                                        caACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTa                                  >  2:325579/1‑141 (MQ=255)

                                                                                                          aCTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAac                                <  1:1001328/141‑1 (MQ=255)

                                                                                                             ggCAAGAAAATTGTGAAGATAATTACCCCAATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAAcaca                             <  1:666346/141‑1 (MQ=255)

                                                                                                             ggCAAGAAAATCGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGAGTACAAACGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAACTGTGGTACCAATGTTATCGCCTAGTAAcaca                             >  2:1171140/1‑141 (MQ=255)

                                                                                                              gCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAg                            >  4:13999/1‑141 (MQ=255)

                                                                                                              gCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAg                            >  4:460810/1‑141 (MQ=255)

                                                                                                               cAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAgg                           <  1:1186340/141‑1 (MQ=255)

                                                                                                               cAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAgg                           >  2:1033196/1‑141 (MQ=255)

                                                                                                                  gAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGAt                        <  1:703730/141‑1 (MQ=255)

                                                                                                                    aaaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGTCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtg                      >  1:537731/1‑141 (MQ=255)

                                                                                                                     aaTTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  1:974402/141‑1 (MQ=255)

                                                                                                                     aaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  1:457284/141‑1 (MQ=255)

                                                                                                                     aaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     >  1:856255/1‑141 (MQ=255)

                                                                                                                     aaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGAATGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  2:301348/141‑1 (MQ=255)

                                                                                                                      aTTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct                    <  2:273738/141‑1 (MQ=255)

                                                                                                                      aTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct                    <  1:445070/141‑1 (MQ=255)

                                                                                                                      aTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct                    <  4:437801/141‑1 (MQ=255)

                                                                                                                       ttGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgctg                   >  4:442406/1‑141 (MQ=255)

                                                                                                                       ttGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgctg                   >  1:997414/1‑141 (MQ=255)

                                                                                                                       ttGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgctg                   >  2:880071/1‑141 (MQ=255)

                                                                                                                         gtgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCg                 <  1:295380/141‑1 (MQ=255)

                                                                                                                         gtgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGACGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCg                 >  3:416685/1‑141 (MQ=255)

                                                                                                                          tgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGt                >  2:971321/1‑141 (MQ=255)

                                                                                                                          tgAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGt                <  2:422547/141‑1 (MQ=255)

                                                                                                                            aaGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGttt              <  1:511961/141‑1 (MQ=255)

                                                                                                                             aGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTa             >  1:190692/1‑141 (MQ=255)

                                                                                                                                tAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGc          <  1:1044017/141‑1 (MQ=255)

                                                                                                                                tAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGc          <  2:809637/141‑1 (MQ=255)

                                                                                                                                 aaTTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCt         <  1:879333/141‑1 (MQ=255)

                                                                                                                                   ttACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTaa       <  2:318935/141‑1 (MQ=255)

                                                                                                                                     aCCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAAtt     >  1:791941/1‑141 (MQ=255)

                                                                                                                                     aCCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATAGAGCCGGCTAAACTAGCTAAGAAAGATGTAATCGTGGCACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAAtt     <  1:1010436/141‑1 (MQ=255)

                                                                                                                                        ccGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGCTTAAGCTAATTaaa  >  1:592712/1‑141 (MQ=255)

TTGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAATTAAA  >  USA300TCH1516_ALE/119716‑119992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`