| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 253,983 | A→G | 6.7% | intergenic (+196/+166) | asbF → / ← USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 253,983 | 0 | A | G | 6.7% | 399.6 / 13.7 | 134 | intergenic (+196/+166) | asbF/USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (64/60); new base G (2/7); total (67/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
GAATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA > USA300TCH1516_ALE/253842‑254121 | gAATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTAC‑TTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAaga < 2:881639/141‑1 (MQ=255) aaTAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAaga < 1:374534/141‑1 (MQ=255) tAAATGCATAAGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATAAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTCTATAGCGATGGTCATACCCATTCACAGTAACAATACTCACCATTGACAAGAGt > 2:59247/1‑141 (MQ=255) aTGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGtata < 3:268108/141‑1 (MQ=255) cATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATaa > 3:65614/1‑141 (MQ=255) aatatGTCGCCAAGGCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCCATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATaa < 2:1120569/140‑1 (MQ=255) atatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAc > 3:134157/1‑141 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc < 1:216703/141‑1 (MQ=255) tatGTCGCCAAGCCATGGCCCAAAATTTCACACCTTAAATCCAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc < 4:501881/141‑4 (MQ=255) tCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt < 1:585871/141‑1 (MQ=255) tCGCCAAGACATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCGCACCATTGAAAAGAGTATATAACCtttt < 4:36941/141‑1 (MQ=255) gCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCa < 4:339309/141‑1 (MQ=255) gCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCa > 3:482455/1‑141 (MQ=255) ccAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCaa < 2:1015257/141‑1 (MQ=39) aGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCAAtgg < 4:319542/141‑3 (MQ=38) ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg > 2:329166/1‑141 (MQ=255) cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa < 2:863017/141‑1 (MQ=255) cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa < 2:995981/141‑1 (MQ=255) aTTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAg > 2:88209/1‑141 (MQ=255) ttGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg < 2:924286/141‑1 (MQ=255) ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat < 1:12874/141‑1 (MQ=255) ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat < 1:881785/141‑1 (MQ=255) aaaaaTTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTatat > 2:1002931/1‑141 (MQ=255) aaTTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGat < 3:116295/141‑1 (MQ=255) tttCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa < 1:114902/141‑1 (MQ=255) cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACTCATTCACAGGAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaat‑aa > 3:400312/1‑141 (MQ=255) cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaat‑aa > 2:418813/1‑141 (MQ=255) gcacCTTAAATTAAGTCATTGGTTGTAAATAATGTTTAATTTATAAAATTATATAGCGATTTTCATACCCATGCACAGTAACAATCCTCACCATTGAAAAGAGTATATAATCTTTTTTATTTTTAGTTATATATTAAT‑aaa > 4:427243/2‑141 (MQ=255) acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATATT‑aaa > 1:1028267/1‑141 (MQ=255) acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaa > 1:1023478/1‑141 (MQ=255) tAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAg < 1:968865/141‑1 (MQ=255) aaTCAAGACATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGcc < 2:180618/141‑1 (MQ=255) tCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCtg < 3:29418/141‑1 (MQ=255) gTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCtgttgt < 1:622918/141‑1 (MQ=255) gTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCtgttgt > 2:859191/1‑141 (MQ=255) ttgtttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTcaca < 3:502538/141‑1 (MQ=255) tttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATg > 1:454771/1‑141 (MQ=255) gagaaaaGAAGGTGTACTTTCTATAAGTATCTAGAGATCGTCATACCCATTCAGAGAAACAAACCTCACCAATGAAAAGAGTATATAACCTTTTCGATAGGAGGGTATATGAAAAA‑AAAAAAAAGCCTGTTGTCACAATgg < 2:1037057/137‑1 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCGTTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTTTCACAATGGTc > 4:162900/1‑141 (MQ=255) tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCa < 2:425216/141‑1 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAACGAGTCAATCACATTATCAATAGTAAAGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCAt > 1:174634/1‑141 (MQ=255) ggTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGacac > 3:394504/1‑141 (MQ=255) gtgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACg < 1:212201/141‑1 (MQ=255) tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa < 1:476149/141‑1 (MQ=255) tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa < 1:51713/141‑1 (MQ=255) tatataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACtt > 3:132100/1‑141 (MQ=255) tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa < 1:191843/141‑1 (MQ=255) tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa > 1:435974/1‑141 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa > 2:534849/1‑72 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa < 1:534849/72‑1 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg > 1:303321/1‑141 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg < 1:320364/141‑1 (MQ=255) tataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAATAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTc < 4:394563/141‑1 (MQ=255) tataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTc < 4:394485/141‑1 (MQ=255) ataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCt > 1:91273/1‑141 (MQ=255) taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTCTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTg > 1:936703/1‑141 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata > 4:34958/1‑141 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata > 2:860093/1‑141 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGAATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata > 3:417978/1‑141 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGAATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCAGTTGTCACAATGGTCATAGACACTACATACTTTAAAGTTTTCTGatta > 3:417970/1‑141 (MQ=255) aTGGTCATACCCATTAACACAAAAAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAAAAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata < 4:176160/141‑1 (MQ=255) ggTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataa < 4:235771/141‑1 (MQ=255) gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataat > 1:213399/1‑141 (MQ=255) gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaagataat > 1:842695/1‑141 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTTTAaatatacta > 1:1004918/1‑141 (MQ=255) cATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataatat < 3:395107/141‑1 (MQ=255) aTACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATAtt > 2:686513/1‑141 (MQ=255) ttCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAAt < 3:298558/141‑1 (MQ=255) tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg < 1:568373/141‑1 (MQ=255) acacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGGATATAATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg < 2:1037141/140‑1 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGc > 4:598/1‑141 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATATCAGAATGc > 2:298228/1‑141 (MQ=255) aGTAACAATCCTCACTATTGAAAAGAGTATATAACCTTTTCAATAGTGACGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACAAGACATACTTTCAAGATTTCTGAATATAATATTTCAGAATGCACt > 4:242045/1‑141 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATCGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt > 2:451730/1‑141 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt > 3:273854/1‑141 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt > 4:370000/1‑141 (MQ=255) aaCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCCGTAGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTAAATATAATATTTCAGAATGCACTTTa > 1:416059/1‑141 (MQ=255) aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATgg < 2:263325/83‑1 (MQ=255) aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATCGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGACTGCACTTTaa > 2:1113044/1‑141 (MQ=255) aactgcTCGCCATTGAAAAGAGTATATAACCTTTTAAATAGTGAAGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTaaa < 4:400312/136‑1 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa > 1:397964/1‑141 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa > 1:874740/1‑141 (MQ=255) ccTCACCATTGAAAAGATTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATg < 2:1180690/141‑1 (MQ=255) cACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAGAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGAc < 1:860093/141‑1 (MQ=255) aCCATTGAAAAGAGTATATAACCTTATCAATAGTGAGGCATATGATAAT‑AGAAAAAAGCCCGTTGCCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATACTTCAGAATGCACTTTAAAGATGGACg < 1:391562/141‑1 (MQ=255) cATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTc < 1:1153432/141‑1 (MQ=255) aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg > 1:89742/1‑141 (MQ=255) aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg > 1:14435/1‑141 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa < 2:803724/141‑1 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa < 2:803779/141‑1 (MQ=255) tGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGAt > 2:1033709/1‑141 (MQ=255) gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa > 1:733486/1‑129 (MQ=39) gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa < 2:733486/129‑1 (MQ=39) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt > 2:1109983/1‑141 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt > 4:56990/1‑141 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt < 3:267377/141‑1 (MQ=255) aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATACT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAg > 1:632102/1‑141 (MQ=255) agagTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa < 1:785186/141‑1 (MQ=255) agagTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa > 1:1188280/1‑141 (MQ=255) agTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa < 1:660829/83‑1 (MQ=255) | GAATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA > USA300TCH1516_ALE/253842‑254121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |