| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 253,984 | G→T | 7.1% | intergenic (+197/+165) | asbF → / ← USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 253,984 | 0 | G | T | 7.1% | 455.0 / 14.8 | 134 | intergenic (+197/+165) | asbF/USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (65/59); new base T (2/8); total (67/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
ATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA > USA300TCH1516_ALE/253844‑254121 | tAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGt > 3:466488/1‑141 (MQ=255) tAAATGCATAAGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATAAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTCTATAGCGATGGTCATACCCATTCACAGTAACAATACTCACCATTGACAAGAGt > 2:59247/1‑141 (MQ=255) aaaTGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGta < 4:194537/141‑1 (MQ=255) cATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATaa > 3:65614/1‑141 (MQ=255) aatatGTCGCCAAGGCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCCATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATaa < 2:1120569/140‑1 (MQ=255) atatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAc > 3:412605/1‑141 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc < 1:354770/141‑4 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc < 1:774899/141‑4 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc < 2:840474/141‑4 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc < 1:897295/141‑1 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc < 1:216703/141‑1 (MQ=255) tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc < 1:624675/141‑1 (MQ=255) tatGTCGCCAAGCCATGGCCCAAAATTTCACACCTTAAATCCAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc < 4:501881/141‑4 (MQ=255) tCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt < 1:585871/141‑1 (MQ=255) tCGCCAAGACATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCGCACCATTGAAAAGAGTATATAACCtttt < 4:36941/141‑1 (MQ=255) cGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc < 1:583259/141‑1 (MQ=255) cGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc < 1:399652/141‑1 (MQ=255) gCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCa < 4:339309/141‑1 (MQ=255) gCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCa > 3:482455/1‑141 (MQ=255) ccAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCaa < 2:1015257/141‑1 (MQ=39) aGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCAAtgg < 4:319542/141‑3 (MQ=38) ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg > 2:329166/1‑141 (MQ=255) ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCAATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg > 2:913871/1‑141 (MQ=255) cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa < 2:863017/141‑1 (MQ=255) ttGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg < 2:924286/141‑1 (MQ=255) tGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGt > 1:498613/1‑141 (MQ=255) ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat < 1:12874/141‑1 (MQ=255) aaTTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGat < 3:116295/141‑1 (MQ=255) tttCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa < 1:114902/141‑1 (MQ=255) cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACTCATTCACAGGAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaataa > 3:400312/1‑141 (MQ=255) cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaataa > 2:418813/1‑141 (MQ=255) cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTGTCAATAGTGAGGTATATGAtaataa < 1:930164/141‑1 (MQ=255) gcacCTTAAATTAAGTCATTGGTTGTAAATAATGTTTAATTTATAAAATTATATAGCGATTTTCATACCCATGCACAGTAACAATCCTCACCATTGAAAAGAGTATATAATCTTTTTTATTTTTAGTTATATATTAATaaa > 4:427243/2‑141 (MQ=255) acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATATTaaa > 1:1028267/1‑141 (MQ=255) acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaa > 1:1023478/1‑141 (MQ=255) ttAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaaaaaaa < 3:288333/141‑1 (MQ=255) tAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAg < 1:968865/141‑1 (MQ=255) aaTCAAGACATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGcc < 2:180618/141‑1 (MQ=255) tCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtg < 3:29418/141‑1 (MQ=255) cAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgt > 2:1003247/1‑141 (MQ=255) ttgtttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTcaca < 3:502538/141‑1 (MQ=255) tttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATg > 1:454771/1‑141 (MQ=255) gagaaaaGAAGGTGTACTTTCTATAAGTATCTAGAGATCGTCATACCCATTCAGAGAAACAAACCTCACCAATGAAAAGAGTATATAACCTTTTCGATAGGAGGGTATATGAAAAAAAAAAAAAGCCTGTTGTCACAATgg < 2:1037057/137‑1 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCGTTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTTTCACAATGGTc > 4:162900/1‑141 (MQ=255) tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCa < 2:425216/141‑1 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAACGAGTCAATCACATTATCAATAGTAAAGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt > 1:174634/1‑141 (MQ=255) gaagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGa > 1:210804/1‑141 (MQ=255) aagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGac > 4:418401/1‑141 (MQ=255) ggTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGacac > 3:394504/1‑141 (MQ=255) tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa < 1:51713/141‑1 (MQ=255) tatataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACtt > 3:132100/1‑141 (MQ=255) tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa > 1:435974/1‑141 (MQ=255) tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa < 1:191843/141‑1 (MQ=255) ataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaa > 2:86469/1‑141 (MQ=255) tcaatatataGCGTTGGTAAAACCCATTTACCGTCACAATCCTCACCATTGAAAGGAGCATATAATCTTTTCAATATTGCGGTATATGATAATAAAAAATAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaaa < 4:365515/137‑1 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa > 2:534849/1‑72 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa < 1:534849/72‑1 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg < 1:320364/141‑1 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg > 1:303321/1‑141 (MQ=255) tataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAATAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTc < 4:394563/141‑1 (MQ=255) tataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTc < 4:394485/141‑1 (MQ=255) taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTCTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTg > 1:936703/1‑141 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata > 2:860093/1‑141 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGAATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCAGTTGTCACAATGGTCATAGACACTACATACTTTAAAGTTTTCTGatta > 3:417970/1‑141 (MQ=255) ggTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataa < 3:213851/141‑1 (MQ=255) ggTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataa < 4:235771/141‑1 (MQ=255) gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataat > 1:213399/1‑141 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTTTAaatatacta > 1:1004918/1‑141 (MQ=255) aTACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATAtt > 2:686513/1‑141 (MQ=255) tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg < 1:454318/141‑1 (MQ=255) tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg < 1:857028/141‑1 (MQ=255) acacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGGATATAATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg < 2:1037141/140‑1 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGc > 4:598/1‑141 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATATCAGAATGc > 2:298228/1‑141 (MQ=255) aGTAACAATCCTCACTATTGAAAAGAGTATATAACCTTTTCAATAGTGACGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACAAGACATACTTTCAAGATTTCTGAATATAATATTTCAGAATGCACt > 4:242045/1‑141 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATCGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt > 2:451730/1‑141 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt > 4:370000/1‑141 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt > 3:273854/1‑141 (MQ=255) tAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTGGTGTCAATGGTCATGGACACGACATGCTTTAATGGTTTCTGGATATAATATTTCAGAATTTACttt > 3:299874/1‑141 (MQ=255) aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATgg < 2:263325/83‑1 (MQ=255) aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATCGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGACTGCACTTTaa > 2:1113044/1‑141 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa > 1:397964/1‑141 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa > 1:874740/1‑141 (MQ=255) ccTCACCATTGAAAAGATTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATg < 2:1180690/141‑1 (MQ=255) cACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAGAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGAc < 1:860093/141‑1 (MQ=255) aCCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACg > 3:117596/1‑141 (MQ=255) cATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTc < 1:1153432/141‑1 (MQ=255) aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg > 1:14435/1‑141 (MQ=255) aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg > 1:89742/1‑141 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa < 2:803724/141‑1 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa < 2:803779/141‑1 (MQ=255) tGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGAt > 2:1033709/1‑141 (MQ=255) gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa < 2:733486/129‑1 (MQ=39) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt > 2:1109983/1‑141 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt > 4:56990/1‑141 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt < 3:267377/141‑1 (MQ=255) aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATACTAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAg > 1:632102/1‑141 (MQ=255) aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATgcag > 1:1156103/1‑138 (MQ=255) agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa < 1:785186/141‑1 (MQ=255) agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa < 1:660829/83‑1 (MQ=255) | ATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA > USA300TCH1516_ALE/253844‑254121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |