BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	328,487	C→A	11.3%	I210I (ATC→ATA)	USA300HOU_RS01470 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	328,487	0	C	A	11.3%	410.1 / 32.1	130	I210I (ATC→ATA)	USA300HOU_RS01470	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (58/57); new base A (8/7); total (66/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01

Only 100 of 135 total aligned reads displayed.

TATATAAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGTTTTTC  >  USA300TCH1516_ALE/328347‑328627

tatataAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATc                                                                                                                                              <  1:430625/141‑1 (MQ=255)

 atataAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCg                                                                                                                                             <  2:260529/141‑1 (MQ=255)

 atataAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCg                                                                                                                                             <  4:161130/141‑1 (MQ=255)

    taAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGaaa                                                                                                                                          <  2:1090563/141‑1 (MQ=255)

    taAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGaaa                                                                                                                                          >  3:472625/1‑141 (MQ=255)

     aaGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGaaaa                                                                                                                                         <  2:830170/141‑1 (MQ=255)

     aaGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGaaaa                                                                                                                                         <  2:103649/141‑1 (MQ=255)

      aGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAAt                                                                                                                                        >  2:1024946/1‑141 (MQ=255)

       gTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATg                                                                                                                                       <  1:18541/141‑1 (MQ=255)

           aaTAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAgg                                                                                                                                   >  2:667180/1‑141 (MQ=255)

                ttCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGa                                                                                                                              <  1:855120/141‑1 (MQ=255)

                 tCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGAc                                                                                                                             >  1:306247/1‑141 (MQ=255)

                 tCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGAc                                                                                                                             >  4:331775/1‑141 (MQ=255)

                  ccTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACa                                                                                                                            <  2:474924/141‑1 (MQ=255)

                   cTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACaa                                                                                                                           >  2:184510/1‑141 (MQ=255)

                   cTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACaa                                                                                                                           >  1:192991/1‑141 (MQ=255)

                    tGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAAt                                                                                                                          <  1:1159933/141‑1 (MQ=255)

                     gTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATg                                                                                                                         <  3:201587/141‑1 (MQ=255)

                        acacAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTaa                                                                                                                      >  3:363864/1‑141 (MQ=255)

                           caGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAgg                                                                                                                   <  2:925224/141‑1 (MQ=255)

                              cAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGaaa                                                                                                                >  2:37309/1‑141 (MQ=255)

                                  aagaGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACa                                                                                                            <  2:1184409/141‑1 (MQ=255)

                                     tgaTTTTTATAGGGTTAAGTTTATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATaa                                                                                                         <  1:10015/140‑1 (MQ=255)

                                     agaTTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATaa                                                                                                         >  2:2667/1‑141 (MQ=255)

                                      gaTTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATaaa                                                                                                        >  2:923758/1‑141 (MQ=255)

                                          tttATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAaat                                                                                                    <  2:1162181/141‑1 (MQ=255)

                                           ttATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAaata                                                                                                   >  4:178856/1‑141 (MQ=255)

                                            tataGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAaataa                                                                                                  >  1:1100008/1‑141 (MQ=255)

                                            tataGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAaataa                                                                                                  >  1:1100074/1‑141 (MQ=255)

                                              taGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAataata                                                                                                <  3:227907/141‑1 (MQ=255)

                                                gggTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAgg                                                                                              >  3:505405/1‑141 (MQ=255)

                                                  gTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAg                                                                                            >  2:70533/1‑141 (MQ=255)

                                                  gTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAg                                                                                            >  2:803225/1‑141 (MQ=255)

                                                   ttAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGt                                                                                           >  2:88918/1‑141 (MQ=255)

                                                      aGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTa                                                                                        <  3:164021/141‑1 (MQ=255)

                                                         atatTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAAcc                                                                                     <  1:735383/141‑1 (MQ=255)

                                                          tatTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCa                                                                                    >  2:211280/1‑141 (MQ=255)

                                                          tatTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCa                                                                                    >  2:806271/1‑141 (MQ=255)

                                                           atTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTGTAACCAt                                                                                   <  1:946267/141‑1 (MQ=255)

                                                            ttgatgaaTATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTGACCATg                                                                                  >  2:104288/7‑141 (MQ=255)

                                                            ttCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATg                                                                                  >  1:25416/1‑141 (MQ=255)

                                                             tcatcaATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATACGAGTATTAAGAAAGATATCGAAAATGAAGGTAACACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGa                                                                                 <  1:254920/141‑1 (MQ=255)

                                                             tcatcaATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGa                                                                                 <  4:470842/141‑1 (MQ=255)

                                                              catcaATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGGAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGAt                                                                                >  4:432936/1‑141 (MQ=255)

                                                                tcaATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATaa                                                                              >  3:75441/1‑141 (MQ=255)

                                                                tcaATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTATAACCATGATaa                                                                              >  4:375499/1‑141 (MQ=255)

                                                                  aaTATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGGTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAAtt                                                                            <  1:134004/141‑1 (MQ=255)

                                                                   atatAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAAttt                                                                           <  1:2463/141‑1 (MQ=255)

                                                                    tataATTGTTTATGATCTTTTTGTTAGGTCATATTTTAAATGCGCGTTATATAAGAGTATTAAGAAAGATAACGAAAAGGAAGGTAAGCCAATGTAAAAAAAAAAAAAAAAAAATAATAGGAGTCTAACCATGATAAtttt                                                                          <  2:94205/141‑1 (MQ=255)

                                                                    tataATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAAtttt                                                                          >  1:691277/1‑141 (MQ=255)

                                                                    tataATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAACGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAAtttt                                                                          >  2:928025/1‑141 (MQ=255)

                                                                    tataATGGTTGATGCGCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAAtttt                                                                          <  1:441569/141‑1 (MQ=255)

                                                                      taATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATtttta                                                                        >  2:1118810/1‑141 (MQ=255)

                                                                      taATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATtttta                                                                        <  3:55076/141‑1 (MQ=255)

                                                                          tgttgATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATttttatttt                                                                    <  1:400377/141‑1 (MQ=255)

                                                                          tgttgATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGaa                                                                                                                 <  3:250283/96‑1 (MQ=255)

                                                                             tgATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAAAGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTtaaa                                                                 >  2:575788/1‑140 (MQ=255)

                                                                              gATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTtaata                                                                <  1:477519/141‑1 (MQ=255)

                                                                                tGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAAc                                                              >  2:201017/1‑141 (MQ=255)

                                                                                tGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAAc                                                              <  1:1158341/141‑1 (MQ=255)

                                                                                 gCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACa                                                             >  1:177469/1‑141 (MQ=255)

                                                                                 gCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACa                                                             <  2:58398/141‑1 (MQ=255)

                                                                                       cttGTTAGGTCATATTTTAATTACGTGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATAttt                                                       <  1:667969/141‑1 (MQ=255)

                                                                                       cttGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATAttt                                                       >  1:537669/1‑141 (MQ=255)

                                                                                        ttGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTg                                                      <  1:302053/141‑1 (MQ=255)

                                                                                         tGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGc                                                     <  2:1110160/141‑1 (MQ=255)

                                                                                          gTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCt                                                    >  2:957527/1‑141 (MQ=255)

                                                                                           ttAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCta                                                   >  1:98885/1‑141 (MQ=255)

                                                                                              ggTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCtattt                                                >  1:413900/1‑141 (MQ=255)

                                                                                              ggTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCtattt                                                <  1:752990/141‑1 (MQ=255)

                                                                                                 cATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAACTGACGATAAGGCTTTATTTATTAAAGAAAATAAAATGAATATGATGCTAACCATGATAATTTTTATTTTAATAACAATATTTGCtatttatt                                             >  1:236624/1‑141 (MQ=255)

                                                                                                  acatgttAATTACGCGTGATATAAGAGTATTAAGACAGATATCGAATATGATGGCAAGTTGATGTAAAGGAAAAACATAACCAATAATAGATGGTTTACCATGATAATTTTTATTTTAATATCAACGTTTGCTATttatta                                            <  4:317917/136‑1 (MQ=255)

                                                                                                     ttttAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATtatat                                         <  2:243586/141‑1 (MQ=255)

                                                                                                     ttttAATTACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATtatat                                         <  3:442203/141‑1 (MQ=255)

                                                                                                      tttAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATtatata                                        >  2:252027/1‑141 (MQ=255)

                                                                                                        tAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGc                                      <  1:883735/141‑1 (MQ=255)

                                                                                                         aaTTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCt                                     >  1:33720/1‑141 (MQ=255)

                                                                                                           ttACGCGTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTAt                                   >  4:423262/1‑141 (MQ=255)

                                                                                                              cgcgTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGAt                                >  3:495752/1‑141 (MQ=255)

                                                                                                              cgcgTTATATAAGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGAt                                <  1:60611/141‑1 (MQ=255)

                                                                                                                cgTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTg                              <  4:356359/141‑1 (MQ=255)

                                                                                                                 gTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGc                             <  1:644847/141‑1 (MQ=255)

                                                                                                                        aaGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAgtttg                      >  1:518288/1‑141 (MQ=255)

                                                                                                                        aaGAGTATTAAGAAAGATATAGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAgtttg                      >  1:1056450/1‑141 (MQ=255)

                                                                                                                          gagTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATGAAAAATAATTGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAgtttgtt                    >  4:379873/1‑141 (MQ=255)

                                                                                                                            gTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTa                  <  1:299261/141‑1 (MQ=255)

                                                                                                                               ttAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTTTTTTTTTTTCAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTaaaa               >  2:577337/1‑141 (MQ=255)

                                                                                                                                  agaaagaTATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAATACATAATAGCCGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTg            <  1:112848/141‑1 (MQ=255)

                                                                                                                                  agaaagaTATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTg            >  2:218602/1‑141 (MQ=255)

                                                                                                                                   gaaagaTATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGa           <  2:30931/141‑1 (MQ=255)

                                                                                                                                      agaTATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTGACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAgg        <  1:104288/141‑1 (MQ=255)

                                                                                                                                        atatCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGtt      >  1:269605/1‑141 (MQ=255)

                                                                                                                                         tatCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGttt     <  1:1178650/141‑1 (MQ=255)

                                                                                                                                          atCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGTGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGtttt    <  2:1110092/141‑1 (MQ=255)

                                                                                                                                          atCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGtttt    <  2:115559/141‑1 (MQ=255)

                                                                                                                                           tCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGttttt   >  3:231234/1‑141 (MQ=255)

                                                                                                                                           tCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGttttt   >  2:595976/1‑141 (MQ=255)

                                                                                                                                           tCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGttttt   <  2:1064371/141‑1 (MQ=255)

                                                                                                                                            cGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGTTTTTc  <  1:13883/141‑1 (MQ=255)

                                                                                                                                            cGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGTTTTTc  >  1:275006/1‑141 (MQ=255)

TATATAAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGGTTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTACGCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAAAGGAAAGACATAAAAAATAATAGGAGTCTAACCATGATAATTTTTATTTTAATAACAATATTTGCTATTTATTATATAGCTATGATTGCTAGTTTGTTTAAAAGTGAAGGTTTTTC  >  USA300TCH1516_ALE/328347‑328627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`