BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	5,952	C→T	9.2%	R298C (CGT→TGT)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	5,952	0	C	T	9.2%	522.0 / 28.7	173	R298C (CGT→TGT)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base C (76/81); new base T (9/7); total (85/88)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 174 total aligned reads displayed.

CAATTTATATTCATCAATCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGATCCTCAATTCGA  >  USA300TCH1516_ALE/5812‑6091

     tataTTCATCAATCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCa                                                                                                                                        <  2:1002938/141‑1 (MQ=255)

     tataTTCATCAATCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCa                                                                                                                                        <  2:978252/141‑1 (MQ=255)

              caATCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCgt                                                                                                                               <  2:1109856/141‑1 (MQ=255)

               aaTCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCgtg                                                                                                                              >  2:255386/1‑141 (MQ=255)

               aaTCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCgtg                                                                                                                              >  4:257013/1‑141 (MQ=255)

                 tCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATCTAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTc                                                                                                                            >  1:644854/1‑141 (MQ=255)

                 tCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTc                                                                                                                            >  4:31473/1‑141 (MQ=255)

                  cTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATCCGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCt                                                                                                                           <  4:185829/141‑1 (MQ=255)

                  cTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCt                                                                                                                           <  1:6472/141‑1 (MQ=255)

                    aaaGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGGGTGTCTTa                                                                                                                         <  1:6112/141‑1 (MQ=255)

                     aaGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACACATCTTTTAACTTACGCAAATAACATTCATACGCATGAAGGTGGTACGCATGAAGACGGCTTCCAACGTGGATGAACGCGTGTCTTaa                                                                                                                        >  1:42054/1‑141 (MQ=255)

                     aaGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTaa                                                                                                                        >  3:178102/1‑141 (MQ=255)

                     aaGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTaa                                                                                                                        >  2:1174293/1‑141 (MQ=255)

                     aaGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTaa                                                                                                                        >  2:876387/1‑141 (MQ=255)

                     aaGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTaa                                                                                                                        >  1:898951/1‑141 (MQ=255)

                      aGATGATATTGAAGTATAAATTTCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTaaa                                                                                                                       <  1:665050/141‑1 (MQ=255)

                      aGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTaaa                                                                                                                       >  4:236590/1‑141 (MQ=255)

                       gatgatATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAAt                                                                                                                      <  1:578725/141‑1 (MQ=255)

                         tgatATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAg                                                                                                                    <  2:1063815/141‑1 (MQ=255)

                         tgatATTGAAGTAGAAAGTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAg                                                                                                                    <  2:493785/141‑1 (MQ=255)

                          gatATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGGGTGTCTTAAATAGt                                                                                                                   >  1:770588/1‑141 (MQ=255)

                            tatTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTa                                                                                                                 <  2:691621/141‑1 (MQ=255)

                            tatTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTa                                                                                                                 >  1:1193903/1‑141 (MQ=255)

                                 aaGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGtt                                                                                                            <  3:377304/141‑1 (MQ=255)

                                   gTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTa                                                                                                          >  3:107312/1‑141 (MQ=255)

                                    tAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTaa                                                                                                         <  1:1066675/141‑1 (MQ=255)

                                     aGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAg                                                                                                        <  2:772592/141‑1 (MQ=255)

                                      gAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGt                                                                                                       >  1:86830/1‑141 (MQ=255)

                                        aaTTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAg                                                                                                     <  1:67753/141‑1 (MQ=255)

                                         aTTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGc                                                                                                    <  2:36590/141‑1 (MQ=255)

                                          ttGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCa                                                                                                   >  2:1039669/1‑141 (MQ=255)

                                            gCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAg                                                                                                 >  1:747568/1‑141 (MQ=255)

                                                ttCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTa                                                                                             >  2:222153/1‑141 (MQ=255)

                                                  cAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATg                                                                                           >  4:12626/1‑141 (MQ=255)

                                                     tataACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGaaa                                                                                        <  2:814996/141‑1 (MQ=255)

                                                       taACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAaaga                                                                                      >  1:457502/1‑141 (MQ=255)

                                                       taACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAaaga                                                                                      >  2:145141/1‑141 (MQ=255)

                                                        aaCTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTAAGaaaaaa                                                                                     <  2:360930/141‑1 (MQ=255)

                                                         aCTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAAGATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAaagaag                                                                                    >  2:1027445/1‑141 (MQ=255)

                                                         aCTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAaagaag                                                                                    >  1:297191/1‑141 (MQ=255)

                                                         aCTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAaagaag                                                                                    <  1:1096729/141‑1 (MQ=255)

                                                         aCTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAaagaag                                                                                    <  1:530584/141‑1 (MQ=255)

                                                          cccAGGATATGCCACAAATATTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGAATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAagaaga                                                                                   <  4:35325/139‑1 (MQ=255)

                                                           tCAGGATCTGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAgaagaa                                                                                  <  1:554227/141‑1 (MQ=255)

                                                            cAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGaaa                                                                                 <  2:37117/141‑1 (MQ=255)

                                                            cAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGaaa                                                                                 >  1:405980/1‑141 (MQ=255)

                                                              ggATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGaaaaa                                                                               >  2:903819/1‑141 (MQ=255)

                                                                   tGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAg                                                                          >  2:24708/1‑141 (MQ=255)

                                                                    gCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGa                                                                         >  2:53096/1‑141 (MQ=255)

                                                                       acaAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTAAAAAAAAAAAAAAAGATAGACtt                                                                      <  2:786979/141‑1 (MQ=255)

                                                                         aaaTCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTc                                                                    <  2:329096/141‑1 (MQ=255)

                                                                            tCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTgg                                                                 <  2:747568/141‑1 (MQ=255)

                                                                             cTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGt                                                                >  2:1092263/1‑141 (MQ=255)

                                                                             cTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGt                                                                >  4:361313/1‑141 (MQ=255)

                                                                             cTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGt                                                                >  3:425088/1‑141 (MQ=255)

                                                                             cTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGt                                                                >  2:329379/1‑141 (MQ=255)

                                                                             cTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGt                                                                >  3:233796/1‑141 (MQ=255)

                                                                             cTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGt                                                                <  1:13584/141‑1 (MQ=255)

                                                                              ttttAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTg                                                               >  4:141385/1‑141 (MQ=255)

                                                                               tttAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGAc                                                                        <  2:125780/131‑1 (MQ=255)

                                                                                 tAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAg                                                            >  1:1126297/1‑141 (MQ=255)

                                                                                  aaCTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGTCGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGGTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAATATAGACTATCTGGTGAAGa                                                           >  2:65726/1‑141 (MQ=255)

                                                                                  aaCTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGa                                                           >  1:826619/1‑141 (MQ=255)

                                                                                  aaCTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGa                                                           <  2:457502/141‑1 (MQ=255)

                                                                                    cTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATa                                                         <  4:497393/141‑1 (MQ=255)

                                                                                       aCGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATacac                                                      <  1:242001/141‑1 (MQ=255)

                                                                                       aCGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATacac                                                      <  2:1021595/141‑1 (MQ=255)

                                                                                        cGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCCAACGTGCATTAACGCGTGTCTTAAATAGTTATGGCTTAAGTAGCAAGATTATGAAAGAAGAAGAAGATAGACTATCTGGTGAAGATACACg                                                     >  1:143164/1‑141 (MQ=255)

                                                                                        cGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACg                                                     <  1:503933/141‑1 (MQ=255)

                                                                                        cGCAAATAACAGTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACg                                                     <  1:263630/141‑1 (MQ=255)

                                                                                          cAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGCTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACCCGTg                                                   <  2:365496/141‑1 (MQ=255)

                                                                                            aaTAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGaa                                                 <  3:66132/141‑1 (MQ=255)

                                                                                              tAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAgg                                               >  2:742626/1‑141 (MQ=255)

                                                                                                    tCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGAc                                         <  1:1002270/141‑1 (MQ=255)

                                                                                                       tACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGc                                      <  3:242029/141‑1 (MQ=255)

                                                                                                        aCGTATGAAGGTGGTACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCa                                     >  2:641384/1‑141 (MQ=255)

                                                                                                        aCGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAATGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCa                                     <  3:151746/141‑1 (MQ=255)

                                                                                                        aCGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCa                                     <  3:257013/141‑1 (MQ=255)

                                                                                                        aCGTATGAAGGTGGTACGCATGAAGACCGATTCAAACGTGCATTAACGCGTGTCTTAGATAGTTATGGTTTAACTAGCAAGATTATGAAAGAAGAAAAGGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCa                                     <  1:1025118/141‑1 (MQ=255)

                                                                                                              gAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGGGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATtata                               <  2:804343/141‑1 (MQ=255)

                                                                                                               aaGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTatat                              >  2:1083833/1‑141 (MQ=255)

                                                                                                               aaGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTatat                              <  3:237876/141‑1 (MQ=255)

                                                                                                               aaGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTatat                              >  3:6201/1‑141 (MQ=255)

                                                                                                                aGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTAtatc                             >  3:199552/1‑141 (MQ=255)

                                                                                                                aGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTAtatc                             >  2:173880/1‑141 (MQ=255)

                                                                                                                   tggtACGCATGAAGACGGATTCAAATGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTAtatctat                          <  3:481043/141‑1 (MQ=255)

                                                                                                                   tggtACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAGATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTAtatctat                          <  2:1134741/141‑1 (MQ=255)

                                                                                                                     gtACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATAGCTATCa                        >  2:487852/1‑141 (MQ=255)

                                                                                                                        cGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAAc                     <  2:1156256/141‑1 (MQ=255)

                                                                                                                        cGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAAc                     >  1:1011903/1‑141 (MQ=255)

                                                                                                                        cGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAAc                     >  4:441201/1‑141 (MQ=255)

                                                                                                                        cGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAAc                     >  3:134977/1‑141 (MQ=255)

                                                                                                                         gCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACa                    >  1:309031/1‑141 (MQ=255)

                                                                                                                             gAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGt                <  1:682267/141‑1 (MQ=255)

                                                                                                                                gACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGAt             >  2:558912/1‑141 (MQ=255)

                                                                                                                                 aCGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGATc            <  3:365623/141‑1 (MQ=255)

                                                                                                                                  cGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGATcc           >  1:340817/1‑141 (MQ=255)

                                                                                                                                      ttCAGACGTGCATTAACGCGTGTCTTAAATAGGTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAAGTATATCTATCAAACATGGTGATGCTCaa       >  2:596576/1‑141 (MQ=255)

                                                                                                                                      ttCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGATCCTCaa       >  1:316260/1‑141 (MQ=255)

                                                                                                                                      ttCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGATCCTCaa       <  2:991984/141‑1 (MQ=255)

CAATTTATATTCATCAATCTAAAGATGATATTGAAGTAGAAATTGCGATTCAATATAACTCAGGATATGCCACAAATCTTTTAACTTACGCAAATAACATTCATACGTATGAAGGTGGTACGCATGAAGACGGATTCAAACGTGCATTAACGCGTGTCTTAAATAGTTATGGTTTAAGTAGCAAGATTATGAAAGAAGAAAAAGATAGACTTTCTGGTGAAGATACACGTGAAGGTATGACAGCAATTATATCTATCAAACATGGTGATCCTCAATTCGA  >  USA300TCH1516_ALE/5812‑6091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`