BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	503,565	G→A	7.5%	noncoding (20/93 nt)	USA300TCH1516_00452 →	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	503,565	0	G	A	7.5%	290.7 / 9.8	95	noncoding (20/93 nt)	USA300TCH1516_00452	tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (48/39); new base A (2/5); total (51/44)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 102 total aligned reads displayed.

TTTTTTACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCTTTTT  >  USA300TCH1516_ALE/503426‑503705

ttttttACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGCATA‑CCCAAGTCCGGc                                                                                                                                             >  1:1025530/1‑141 (MQ=255)

ttttttACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGc                                                                                                                                             <  2:216257/141‑1 (MQ=255)

ttttttACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGc                                                                                                                                             >  1:840515/1‑141 (MQ=255)

ttttttACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGc                                                                                                                                             >  1:840614/1‑141 (MQ=255)

   tttACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGa                                                                                                                                          <  1:457139/141‑1 (MQ=255)

      aCATTCGTTTATAGGTCAACTGTAGTGGAAGACCATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAgg                                                                                                                                       >  1:618130/1‑141 (MQ=255)

      aCATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAgg                                                                                                                                       <  2:243758/141‑1 (MQ=255)

      aCATTCGTTGATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAgg                                                                                                                                       >  1:1049470/1‑141 (MQ=255)

       cATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAggg                                                                                                                                      >  1:19620/1‑141 (MQ=255)

        aTTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGa                                                                                                                                     <  2:321937/141‑1 (MQ=255)

        aTTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGa                                                                                                                                     <  2:623042/141‑1 (MQ=255)

            gTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCgg                                                                                                                                 <  1:904225/141‑1 (MQ=255)

            gTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAGGGATCgg                                                                                                                                 <  1:221337/141‑1 (MQ=255)

            gTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAGGGATCgg                                                                                                                                 <  1:599472/141‑1 (MQ=255)

            gTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAGGGATCgg                                                                                                                                 <  1:599427/141‑1 (MQ=255)

                ataGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCtt                                                                                                                             <  2:387811/141‑1 (MQ=255)

                 taGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTg                                                                                                                            >  1:370848/1‑141 (MQ=255)

                  aGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGa                                                                                                                           <  1:1111056/141‑1 (MQ=255)

                   ggTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGaa                                                                                                                          <  2:500368/141‑1 (MQ=255)

                   ggTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGaa                                                                                                                          <  2:500503/141‑1 (MQ=255)

                      cAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAAc                                                                                                                       >  1:951800/1‑141 (MQ=255)

                           gTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACa                                                                                                                  <  2:197784/141‑1 (MQ=255)

                             aGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATACCCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAg                                                                                                                 >  1:25107/1‑141 (MQ=255)

                             aGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAgg                                                                                                                >  3:438792/1‑141 (MQ=255)

                             aGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAgg                                                                                                                >  2:451790/1‑141 (MQ=255)

                              gTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAGATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAggg                                                                                                               >  2:257134/1‑141 (MQ=255)

                                  aaGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCtt                                                                                                           <  2:797793/141‑1 (MQ=255)

                                   aGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTa                                                                                                          >  1:99558/1‑141 (MQ=255)

                                   aGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTACGGCTGAAGGGATCGGGCTTGAAAACCGACAGGGGCTTa                                                                                                          >  1:554240/1‑141 (MQ=255)

                                    atcAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTaa                                                                                                         <  2:1030354/139‑1 (MQ=255)

                                     aCAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATACCCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTaa                                                                                                         <  2:25107/141‑1 (MQ=255)

                                     aCAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAAc                                                                                                        <  1:1089072/141‑1 (MQ=255)

                                     aCAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCGTGAAAACCGACAGGGGCTTAAc                                                                                                        <  2:1168901/141‑1 (MQ=255)

                                         tGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCt                                                                                                    >  2:590138/1‑141 (MQ=255)

                                          gATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTc                                                                                                   >  2:862701/1‑141 (MQ=255)

                                          gATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTc                                                                                                   <  2:628032/141‑1 (MQ=255)

                                           aTTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTcg                                                                                                  <  1:927043/141‑1 (MQ=255)

                                            tttGTGGTAATCATGTAATGCTTAAAAACAATATTGCCCTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTcgc                                                                                                 >  2:271756/1‑141 (MQ=255)

                                             ttGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTagcg                                                                                                >  3:482890/1‑141 (MQ=255)

                                                tgGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATCAATATTGTGTTTAGGAGGATGA‑CCCAAGTCCGGCTGGAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCgggg                                                                                             >  3:399456/1‑141 (MQ=255)

                                                tgGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCgggg                                                                                             >  1:450709/1‑141 (MQ=255)

                                                tgGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCgggg                                                                                             >  3:221188/1‑141 (MQ=255)

                                                tgGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCgggg                                                                                             >  2:85364/1‑141 (MQ=255)

                                                tgGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCgggg                                                                                             >  2:867807/1‑141 (MQ=255)

                                                tgGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGGTGATATACGATTACGTTGATGTATAGTAATTTGT‑CTCAAGTCCTCCTGAATGGATCTGTCTTGATATCCGACTGGTGCTTAACGGCTCGCgggg                                                                                             >  1:1009387/1‑141 (MQ=255)

                                                 ggTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCggggg                                                                                            <  2:230316/141‑1 (MQ=255)

                                                 ggTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCggggg                                                                                            <  1:57377/141‑1 (MQ=255)

                                                               gCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCtt                                                                              >  2:81923/1‑141 (MQ=255)

                                                                cTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTtc                                                                             <  2:607727/141‑1 (MQ=255)

                                                                  tAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTtccc                                                                           >  2:641772/1‑140 (MQ=255)

                                                                   aaaaaCAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCTCGGGGGTTCGAAttcctcttcctc                                                                          >  1:1142894/1‑141 (MQ=255)

                                                                   aaaaaCAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTtcctc                                                                          >  2:1062094/1‑141 (MQ=255)

                                                                    aaaaCAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTtcctcc                                                                         >  2:529496/1‑141 (MQ=255)

                                                                       aCAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGcc                                                                      <  1:1094456/141‑1 (MQ=255)

                                                                        cAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCa                                                                     >  1:270426/1‑141 (MQ=255)

                                                                          atatTGACTTTTACAGAACGCTCATATATGATAAATATTGTTTTTAGGAGGAATA‑CCAAAGTCCGGCTGAAGGGGTCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATc                                                                   >  2:1183846/1‑141 (MQ=255)

                                                                           tatTGACTTTTACAGAAAGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCa                                                                  >  2:297028/1‑141 (MQ=255)

                                                                              tGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAata                                                               >  1:1056461/1‑141 (MQ=255)

                                                                               gACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAatat                                                              >  2:351112/1‑141 (MQ=255)

                                                                                  ttttACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATAtttc                                                           <  1:1132308/141‑2 (MQ=255)

                                                                                         gAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTaaa                                                    <  2:670695/141‑1 (MQ=255)

                                                                                         gAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTaaa                                                    >  3:359020/1‑141 (MQ=255)

                                                                                           aCGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAAtt                                                  >  1:632509/1‑141 (MQ=255)

                                                                                            cGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTc                                                 <  1:677092/141‑1 (MQ=255)

                                                                                               tCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtat                                              >  2:333894/1‑141 (MQ=255)

                                                                                                cATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtata                                             <  1:1104337/141‑1 (MQ=255)

                                                                                                cATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtata                                             >  2:859349/1‑141 (MQ=255)

                                                                                                  tatatGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtatata                                           <  3:374040/141‑1 (MQ=255)

                                                                                                   atatGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAATCGACAGGGGCTTAACGTCTTTCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATAGTAAATACtagataa                                          >  2:171336/1‑140 (MQ=18)

                                                                                                     atGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATaa                                        <  2:560213/141‑1 (MQ=255)

                                                                                                     atGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGAGCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATaa                                        <  1:582760/141‑1 (MQ=255)

                                                                                                      tGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAAt                                       >  1:59624/1‑141 (MQ=255)

                                                                                                         tAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGaa                                    >  2:55847/1‑141 (MQ=255)

                                                                                                         tAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGaa                                    <  2:618130/141‑1 (MQ=255)

                                                                                                         tAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGACTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGaa                                    <  2:1049470/141‑1 (MQ=255)

                                                                                                           aaTATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGTGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAgg                                  <  4:27302/141‑1 (MQ=255)

                                                                                                            atatTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGt                                 >  2:347212/1‑141 (MQ=255)

                                                                                                            atatTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGt                                 >  2:1039144/1‑141 (MQ=255)

                                                                                                               ttGTGTTTAGGGGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAg                              >  2:1064656/1‑141 (MQ=255)

                                                                                                                 gtgtTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACTGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTACTCCGCCATCCATATTTAAATTAAATACTATATATAATGAAGGGAAggg                            >  1:275198/1‑139 (MQ=18)

                                                                                                                 gtgtTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTg                            >  2:493932/1‑141 (MQ=255)

                                                                                                                  tgtTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGAGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGc                           <  4:221749/141‑1 (MQ=255)

                                                                                                                    tttAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTc                         <  1:973811/141‑1 (MQ=255)

                                                                                                                           ggaATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAAtttt                  >  2:189987/1‑141 (MQ=255)

                                                                                                                             aaTA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCACATGTTGa                >  2:941743/1‑141 (MQ=255)

                                                                                                                              aTA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAg               >  4:272649/1‑141 (MQ=255)

                                                                                                                              aTA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAg               >  1:1067235/1‑141 (MQ=255)

                                                                                                                                  cccAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTAt            <  1:639899/141‑1 (MQ=255)

                                                                                                                                    cAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTAttt          <  2:529735/141‑1 (MQ=255)

                                                                                                                                    cAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTAttt          <  3:412101/141‑1 (MQ=255)

                                                                                                                                     aaGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTa         <  2:771679/141‑1 (MQ=255)

                                                                                                                                     aaGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTa         <  1:543599/141‑1 (MQ=255)

                                                                                                                                       gTCCGGCTGAAGGTATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAAGCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTAcc       <  1:1133960/141‑1 (MQ=255)

                                                                                                                                          cGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCttt    >  4:185728/1‑141 (MQ=255)

                                                                                                                                            gCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCttttt  >  1:699137/1‑141 (MQ=255)

                                                                                                                                            gCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCttttt  >  2:187483/1‑141 (MQ=255)

                                                                                                                                            gCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCttttt  >  1:1030636/1‑141 (MQ=255)

                                                                                                                                            gCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCttttt  >  1:1133778/1‑141 (MQ=255)

                                                                                                                                            gCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATCTTGAGAATTTACCttttt  >  1:269679/1‑141 (MQ=255)

                                                                                                                                            gCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGATCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCttttt  >  1:638029/1‑141 (MQ=255)

TTTTTTACATTCGTTTATAGGTCAACTGTAGTGGAAGACAATGATTTGTGGTAATCATGTAATGCTTAAAAACAATATTGACTTTTACAGAACGTTCATATATGATAAATATTGTGTTTAGGAGGAATA‑CCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATAATGAAGGTAAGTGCTCAAATTTTGAGTATTTACCTTTTT  >  USA300TCH1516_ALE/503426‑503705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`