| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 589,533 | A→T | 8.1% | D1146V (GAT→GTT) ‡ | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 589,533 | 0 | A | T | 8.1% | 428.5 / 22.4 | 135 | D1146V (GAT→GTT) ‡ | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (70/54); new base T (7/4); total (77/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
Only 100 of 137 total aligned reads displayed.
ATCCGATGATACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAACAGGCAAA > USA300TCH1516_ALE/589393‑589672 | aTCCGATGATACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGa > 1:549825/1‑141 (MQ=255)aTCCGATGATACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGa > 1:1004592/1‑141 (MQ=255) tCCGATGATACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGAt < 2:1031519/141‑1 (MQ=255) gatACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATTAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCaa > 4:81899/1‑141 (MQ=255) gatACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCaa > 2:40999/1‑141 (MQ=255) gatACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCaa > 2:126832/1‑141 (MQ=255) atACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAg < 4:50535/141‑1 (MQ=255) ttcAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGa < 1:1165525/139‑1 (MQ=255) tACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGa > 3:109578/1‑141 (MQ=255) aCAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGAt < 1:876740/141‑1 (MQ=255) aGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCacccacc > 2:593614/1‑135 (MQ=255) gTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAAt < 2:930800/141‑1 (MQ=255) tAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATg > 4:208259/1‑141 (MQ=255) ggACGTGCGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGaa > 1:944320/1‑141 (MQ=255) gACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGaaa < 4:430607/141‑1 (MQ=255) gtgAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGaa > 2:1152710/1‑141 (MQ=255) tgAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGaaa > 1:510470/1‑141 (MQ=255) aaaaCATCCGAGGCTATTGTTAAAGGTGAAAGAATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAACGAATTACAAAGTATAGGTTTAGATGTCAAAGTTATGGATGAGCAAGATAATGAAATCGAAATg < 2:713150/141‑1 (MQ=255) aaaaCATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATg > 1:436610/1‑141 (MQ=255) aaaCATACGAGGCCATTGTTAAAGGTGAACACATCACTAGACCACGTGTTCCAGAATCATTCCGAGTATTGCTGAAAGAATTATAAAGGTTAGTTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAGTGa > 2:311261/1‑141 (MQ=255) aaCATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGAc > 2:1037119/1‑141 (MQ=255) cATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAg > 4:257187/1‑141 (MQ=255) aTACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGa < 4:69980/141‑1 (MQ=255) aTACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGa < 2:540765/141‑1 (MQ=255) tACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGAc > 4:254847/1‑141 (MQ=255) gAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGtt > 1:577074/1‑141 (MQ=255) gAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGtt > 4:367676/1‑141 (MQ=255) gAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGtt > 3:448384/1‑141 (MQ=255) aGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTtg < 1:470996/141‑1 (MQ=255) gCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCTAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTtgat < 2:858433/141‑1 (MQ=255) tATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTtgatga < 1:1023182/141‑1 (MQ=255) aTTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGAc > 3:56096/1‑141 (MQ=255) gTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACgat > 2:888521/1‑141 (MQ=255) gTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACgat < 1:210297/141‑1 (MQ=255) aGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGAtgt > 2:874453/1‑141 (MQ=255) aaaaCATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAg < 2:888013/141‑1 (MQ=255) aaaaCAGCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAg < 2:115418/141‑1 (MQ=255) aaCATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGAAGTTGATGACGATGATGTTGTAGaa > 1:261198/1‑141 (MQ=255) aCATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTCCAAAGTGTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAAc < 1:376980/141‑1 (MQ=255) cATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACg < 4:450088/141‑1 (MQ=255) tctcTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGTTTGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCa > 2:498651/1‑141 (MQ=255) tctcTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCa < 1:574077/141‑1 (MQ=255) tctcTAGACCAAGTGTTCCAGAATCAGTACGAGTATTGGTGAAATAATTAAAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGCGCAAGATAATGAAATCGAAATGACAGACGTTAGGGGCGATTATGATGTAAAACGCa < 4:505936/141‑1 (MQ=255) ttAGACCAAGTGTTCCAGCATCATTCCGAGTATTCATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAGAGTTATGCATGAGCAAGATAATGAAAGCGAAATGACAGACGTTGATGACGATGGTGTTGTAGATCGCACAg < 4:445752/140‑1 (MQ=255) ctAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAg < 1:1063438/141‑1 (MQ=255) ctAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAg < 3:343478/141‑1 (MQ=255) tAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGt > 1:640199/1‑141 (MQ=255) aGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTa > 3:224878/1‑141 (MQ=255) aGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTa > 2:805679/1‑141 (MQ=255) aGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTa > 2:873322/1‑141 (MQ=255) aGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTGGAGGTTGAGATGTAAAAGTTATGGATGAGCAAGATAAGGAAATCGAAATGACAGACGTTGATGACGAGGATGGTGTAGAACGCAAAGGAGAttga > 4:249581/1‑139 (MQ=255) gtgtTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTac > 1:1158410/1‑141 (MQ=255) gtgtTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGAATTac > 3:215063/1‑141 (MQ=255) tgtTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTaca < 2:1027819/141‑1 (MQ=255) tgtTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTaca > 2:809646/1‑141 (MQ=255) gtTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTacaa > 4:446151/1‑141 (MQ=255) ccAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTAcaacaa > 2:658906/1‑141 (MQ=255) cAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACaaa < 4:224713/141‑1 (MQ=255) cAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGAAGATGATGTTGTAGAACGCAAAGTAGATTTACAACaaa > 3:408556/1‑141 (MQ=255) gAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAat < 1:202934/141‑1 (MQ=255) aaTCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAatg < 4:344711/141‑1 (MQ=255) cATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAatgatg < 1:973148/141‑1 (MQ=255) ttCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCt > 2:243978/1‑141 (MQ=255) aGTATTGTTGAGAGAATTATAATGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGCAATCGAAATGTCAGACGTTGTTGTCGATGATGTTTTAGAAAGCAAAGTAGATTTACAATAAAATGATGCTCCTGa > 4:167501/1‑141 (MQ=255) aGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGa < 1:626674/141‑1 (MQ=255) gTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGaa > 2:718342/1‑141 (MQ=255) aTTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTAATGaaaa < 2:107964/141‑2 (MQ=255) gatgaAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAAcaca > 2:1105895/1‑141 (MQ=255) atgaAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACaaaa > 4:128678/1‑141 (MQ=255) aGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAaaga < 4:109578/141‑1 (MQ=255) aGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAaaga < 1:483056/141‑1 (MQ=255) tACAAAGTTTAGGTTTAGATGTAAAAGTTATGTTTGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTa > 2:464483/1‑141 (MQ=255) cAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGTCGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGATACACTAAAAGAAGTTACt < 2:1079905/141‑1 (MQ=255) cAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACt > 1:483866/1‑141 (MQ=255) aaaGTTTAGGTTTAGATGTAAAAGTTATGGATGAGTAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATTCTCCTGAAACACAAAAAGAAGTTACta > 2:117362/1‑140 (MQ=255) aaaGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTg < 2:777360/141‑1 (MQ=255) aaaGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTg > 1:881361/1‑141 (MQ=255) atttaggtttagAAGAAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGAAAAAAAAAAAAAAAAAGAAGTTACTGAtt < 2:802923/140‑1 (MQ=255) ttaggtttagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTaa < 2:483866/141‑1 (MQ=255) taggtttagATGTAAAAGTTATGTTTGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAAt < 3:31009/141‑1 (MQ=255) taggtttagATGTAAAAGTTATGTTTGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAAt > 1:831425/1‑141 (MQ=255) taggtttagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAAt > 3:393379/1‑141 (MQ=255) aggtttagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATa < 1:363344/141‑1 (MQ=255) ggtttagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATAc > 2:66784/1‑141 (MQ=255) ggtttagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATAc > 3:301055/1‑141 (MQ=255) tttagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGc < 3:38021/141‑1 (MQ=255) tagATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCaa < 2:1175435/141‑1 (MQ=255) agATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAAt > 3:493070/1‑141 (MQ=255) aTGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAAttt > 2:704847/1‑141 (MQ=255) tAAAAGTTATGTTTGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACa > 1:240291/1‑141 (MQ=255) tAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACa > 1:783450/1‑141 (MQ=255) aaaaGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACaa > 2:796866/1‑141 (MQ=255) aaaaGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACaa > 1:1152781/1‑141 (MQ=255) aaaGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACaaa > 3:422112/1‑141 (MQ=255) aaGTTATGTTTGAGCAAAATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTaaaaaa < 2:941242/141‑1 (MQ=255) aGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGGTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAAc < 1:1005992/141‑1 (MQ=255) tATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAACAgg < 1:591973/141‑1 (MQ=255) aTGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAACAGGc > 3:219482/1‑141 (MQ=255) tGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAACAGGCa > 3:380836/1‑141 (MQ=255) ggATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAACAGGCaa > 2:738841/1‑141 (MQ=255) | ATCCGATGATACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCTAGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTAGATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGATGATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAAGTTACTGATTAATACGCAATTTACAAAACAGGCAAA > USA300TCH1516_ALE/589393‑589672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |