Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,588 G→A 9.5% S1230S (TCG→TCA)  sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5880GA 9.5% 389.0 / 4.9 158S1230S (TCG→TCA) sdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (68/68);  new base A (10/9);  total (79/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 161 total aligned reads displayed.

GCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGA  >  USA300TCH1516_ALE/618449‑618728
                                                                                                                                           |                                                                                                                                            
    ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACATCGACTCGGGCTCAGACAGCGACTCAGACGCAGATAGGGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                                         >  1:1189887/1‑141 (MQ=17)
    ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGTGACTCAGACTCAGATAg                                                                                                                                         >  4:2219/1‑141 (MQ=255)
    ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCGGagag                                                                                                                                         >  4:76728/1‑141 (MQ=255)
      cagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCg                                                                                                                                       <  3:394709/141‑1 (MQ=255)
        gattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGaa                                                                                                                                     >  2:217688/1‑140 (MQ=255)
          ttcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACGCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                                                                                   <  1:573003/141‑1 (MQ=255)
            cagactgcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCCGACAGCGACTCAGACTCAGACAGCGACTCAGCCTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                                                                                 <  2:864759/141‑1 (MQ=255)
              gacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAc                                                                                                                               <  1:1049229/141‑1 (MQ=255)
              gacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                                                               >  2:795116/1‑141 (MQ=255)
                   cgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGAGACTCAGACTCAGATAGAGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                                                          >  4:176836/1‑141 (MQ=11)
                    gacagagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGAt                                                                                                                         >  2:1001413/6‑141 (MQ=255)
                      ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                                       <  3:369785/141‑1 (MQ=255)
                      ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGGGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAg                                                                                                                       >  2:1065206/1‑141 (MQ=255)
                        cagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                                                                                     >  1:724872/1‑141 (MQ=255)
                         agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGCGTCAGATAGCGACTCAGAGTCAGAGAGAGAACAAGAGTCAGACAGTGAGTCAGACTCAGATAgtcg                                                                                                                    >  2:98002/1‑138 (MQ=11)
                         agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                                                    <  1:436987/141‑1 (MQ=255)
                          gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGGCTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAGTCAGACACAGATAACGAc                                                                                                                   >  2:423293/1‑141 (MQ=14)
                          gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                                                   >  1:952248/1‑141 (MQ=255)
                          gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                                                   <  3:189554/141‑1 (MQ=255)
                          gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                                                   >  1:459523/1‑141 (MQ=255)
                               agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                                              <  2:634982/141‑1 (MQ=255)
                                     tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                                        <  1:779585/141‑1 (MQ=255)
                                     tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                                        <  2:835756/141‑1 (MQ=255)
                                      gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                                                       >  3:49588/1‑141 (MQ=255)
                                        ttCAGACTCAGATAGCGACTCAGATTCAGACATCGACTCAGACTCACACAGCGACACAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                     <  4:333234/141‑1 (MQ=255)
                                        ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                     <  3:289744/141‑1 (MQ=255)
                                          cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCg                                                                                                   >  2:675693/1‑141 (MQ=255)
                                           aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                                                  <  2:810296/141‑1 (MQ=255)
                                            gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                                                 <  2:1067931/141‑1 (MQ=255)
                                             aCTCAGATTGCGACTCAGATTCAGACTGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtt                                                                                                <  2:447643/141‑1 (MQ=255)
                                             aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtt                                                                                                >  3:67483/1‑141 (MQ=255)
                                              cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCGGATAGCGactc                                                                                               >  1:305654/1‑138 (MQ=255)
                                                cAGACAGCGACTCAGCTTCACATAGCGATTCAGACTCAGACAGCGTCTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGTCTCAGATAGCGACTCAGATTCAGATAGCGATTCgg                                                                                             <  1:549773/141‑1 (MQ=11)
                                                   aTAGCGACACAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACt                                                                                          <  4:107489/141‑1 (MQ=255)
                                                    caGCGACTCCGATTCAGACCGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                         <  2:67127/140‑1 (MQ=255)
                                                     aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                                        <  2:724872/141‑1 (MQ=255)
                                                       caaCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATCCGGACTCAGa                                                                                      <  2:906509/139‑1 (MQ=255)
                                                          cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                   >  1:844728/1‑141 (MQ=255)
                                                               aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAAAAGGACTTAGCTAGCggcg                                                                              >  2:346487/1‑138 (MQ=14)
                                                               aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                                              <  2:641975/141‑1 (MQ=255)
                                                                 tcagacagcgactcagactcagacagcgactcagacTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtca                                                                            <  4:328407/141‑1 (MQ=255)
                                                                  cagacagcgcctcagactcagacagcgactcagactCAGATAGTGACTCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                                           <  2:1146359/141‑1 (MQ=255)
                                                                    gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                         <  1:93594/141‑1 (MQ=255)
                                                                         cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                    <  3:140077/141‑1 (MQ=255)
                                                                          gactcagagtcagacagcgactcaggctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                                                   <  1:412736/141‑1 (MQ=255)
                                                                          gactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                                                   <  1:315476/141‑1 (MQ=255)
                                                                          gactcagactcagacagcgacgcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                                                   <  1:1123666/141‑1 (MQ=255)
                                                                            ctcagactcaggcagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTAAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                 <  1:1165843/141‑1 (MQ=255)
                                                                            ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                 >  1:1088471/1‑141 (MQ=255)
                                                                            ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATac                                                                 >  1:154923/1‑140 (MQ=255)
                                                                              cagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGAAGCGGGGAGGGACTCAGACTCAGATAGCGACGCAGGTTCAGGTAGAGATCCGGACGCAGACAGCGACTCAGAATCAGATAgcg                                                               >  2:769675/1‑139 (MQ=11)
                                                                                gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGCTAGCGAt                                                             >  2:567906/1‑141 (MQ=255)
                                                                                gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGAGTCAGAGAGCGATTCAGACTCAGATAGCGACTGAGCGTCAGATCGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                             >  2:859377/1‑141 (MQ=14)
                                                                                 actcagacagcggctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                            <  3:120790/141‑1 (MQ=255)
                                                                                  ctcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGGGAATc                                                           >  1:848253/1‑141 (MQ=255)
                                                                                      gacagcgactcagactcagaTAGAGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                                                       <  2:922727/141‑1 (MQ=14)
                                                                                        cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                     <  3:495679/141‑1 (MQ=255)
                                                                                        cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAATCAGACTCAGATAGCGACTCAGATTCAGATAGCGATGCGGACTCAGATAGCGACTCAGGTTCAGATAGTGATTCAGACTc                                                     >  1:297720/1‑141 (MQ=255)
                                                                                         agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                    >  1:64050/1‑141 (MQ=255)
                                                                                          gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                               <  4:446745/113‑1 (MQ=255)
                                                                                          acgactcagactcagaTACTGACACAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGCCTCAGATTCAGATAGTGATTCAGACTCAg                                                   <  4:387961/140‑1 (MQ=14)
                                                                                           cgactcagactccgaTCGTGAAGCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGAGATTCGGACTCAGATAGCGACTCAGGTTCAGATAGTGATTCAGACTCAGa                                                  <  4:176009/141‑1 (MQ=17)
                                                                                           cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                  <  2:79790/141‑1 (MQ=255)
                                                                                              ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                               >  1:1168690/1‑141 (MQ=255)
                                                                                              ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                               <  1:1135323/141‑1 (MQ=255)
                                                                                              ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                               <  2:620656/141‑1 (MQ=255)
                                                                                                cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg                                             >  4:503005/1‑141 (MQ=255)
                                                                                                 agactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGa                                            >  4:262016/1‑141 (MQ=255)
                                                                                                  gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                           <  2:13241/141‑1 (MQ=255)
                                                                                                  gactcagaTAGTGACTCAGATTCCGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                           >  3:483853/1‑141 (MQ=255)
                                                                                                  gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                           <  1:82714/141‑1 (MQ=255)
                                                                                                      cagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTccg                                       >  4:490860/1‑139 (MQ=255)
                                                                                                       agaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa                                      >  3:503397/1‑141 (MQ=255)
                                                                                                            gTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAg                                 >  3:423/1‑141 (MQ=255)
                                                                                                              gACTGAGATTCAGACAGCGACTGAGATTCGGACACCGACTCAGACTCAGATACCGACTCAGATTCAGATGGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                               <  4:393588/141‑1 (MQ=17)
                                                                                                              gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                               >  1:391897/1‑141 (MQ=255)
                                                                                                              gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                               >  1:762696/1‑141 (MQ=255)
                                                                                                                  cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGATAGCGGCTGCGGTTACGATCGTGCTTGCGCCTCAGATAGCGACTCAGATTCAGACAGCg                           >  1:1004995/1‑141 (MQ=17)
                                                                                                                   ggaagcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCATATAGCGTCTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  1:77391/136‑1 (MQ=255)
                                                                                                                   agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  2:844728/141‑1 (MQ=255)
                                                                                                                   agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  2:501527/141‑1 (MQ=255)
                                                                                                                   agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  2:936917/141‑1 (MQ=255)
                                                                                                                   agattcagatAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGACTCAGATTCAGATAGAGATTCGGACTCAGATACCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  2:753837/141‑1 (MQ=255)
                                                                                                                   agattcagatAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  4:223226/141‑1 (MQ=255)
                                                                                                                   agactcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                          <  1:997537/141‑1 (MQ=255)
                                                                                                                    gattcatatAGCGATTTAGTTTCTGATATCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGGGAATCGTGTTCAGATAGCGACCCAGTTTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc                         <  2:355037/141‑1 (MQ=14)
                                                                                                                    gagtcagatAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACCCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc                         <  2:1111231/138‑1 (MQ=255)
                                                                                                                     attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                        <  1:942074/141‑1 (MQ=255)
                                                                                                                       tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGATCCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCa                      <  2:507667/141‑1 (MQ=255)
                                                                                                                        cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                     >  1:1028696/1‑141 (MQ=255)
                                                                                                                          gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAt                   >  3:428249/1‑141 (MQ=255)
                                                                                                                                gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAt             >  3:373071/1‑141 (MQ=255)
                                                                                                                                gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAt             >  3:165271/1‑141 (MQ=255)
                                                                                                                                  cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGCCTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGCCTCAGATTCGGATAg           <  1:1144544/141‑1 (MQ=255)
                                                                                                                                  cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg           >  2:5267/1‑141 (MQ=255)
                                                                                                                                     aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGa        >  1:530392/1‑141 (MQ=255)
                                                                                                                                      gATTCGGACAGCGACTCCGACTCAGATACCGACACAGATTCAGATAGCGAGTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc       <  2:1095753/141‑1 (MQ=17)
                                                                                                                                          cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAg   >  2:883665/1‑141 (MQ=255)
                                                                                                                                          cGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAATCAGACAGCGACTCAGATTCCGGTAGCGACTCAg   >  2:253172/1‑141 (MQ=255)
                                                                                                                                           ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGa  <  1:33151/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGA  >  USA300TCH1516_ALE/618449‑618728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑