Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,759 T→C 6.8% D1287D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,7590TC 6.8% 402.0 / 3.6 148D1287D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (64/72);  new base C (8/4);  total (72/76)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGTACTAC  >  USA300TCH1516_ALE/618619‑618899
                                                                                                                                            |                                                                                                                                            
gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                                                                                                                                              >  1:230553/1‑141 (MQ=255)
  ttcagatAGCGATTCGGCCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGCCTCGGATAGTGATTc                                                                                                                                            >  1:133131/1‑141 (MQ=255)
    cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg                                                                                                                                          >  3:74105/1‑141 (MQ=255)
      gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAc                                                                                                                                        <  1:1109598/141‑1 (MQ=255)
      gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAc                                                                                                                                        >  1:684961/1‑141 (MQ=255)
      gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGAc                                                                                                                                        >  2:1183387/1‑141 (MQ=255)
        tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                                                                                      >  4:129423/1‑141 (MQ=255)
         aGCGATTCAGAATCACAAAGCGACTCAGAATCCGATAGCGATACAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCa                                                                                                                                     <  4:500/141‑1 (MQ=14)
          gCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAg                                                                                                                                    >  3:390828/1‑141 (MQ=255)
                 ggACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGGCAGCGATTCAGACTCGGATAGTGATTCAGACTCAGATAGCga                                                                                                                             <  1:1027697/141‑1 (MQ=255)
                   aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgact                                                                                                                           >  1:643617/1‑141 (MQ=255)
                      cAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAAAGCGAATCAGATTCGGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGTGATTAAGACTCAGATAGCgaataag                                                                                                                        >  2:310839/1‑136 (MQ=17)
                          tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                                                                                                                    <  3:176650/141‑1 (MQ=255)
                          tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                                                                                                                    >  1:406627/1‑141 (MQ=255)
                                cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                                                                                              >  1:1081425/1‑141 (MQ=255)
                                cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                                                                                              <  1:423293/141‑1 (MQ=255)
                                cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAGTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                                                                                              >  2:257667/1‑141 (MQ=255)
                                cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACACAGATAGAGACTAAGAAGCAGATAg                                                                                                              >  2:525758/1‑141 (MQ=17)
                                   agattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCga                                                                                                           <  1:1065206/141‑1 (MQ=255)
                                    gtttcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgat                                                                                                          <  1:1113079/139‑1 (MQ=255)
                                    gattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgat                                                                                                          <  2:142788/141‑1 (MQ=14)
                                    cagtcagagAGAGATTCACACGCAGAAAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgat                                                                                                          <  2:1004995/138‑1 (MQ=17)
                                      ttcagatAGTGATTCAGACTCAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACGCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGAAACCGTCTCAGACTCAGATAGCgatcc                                                                                                        >  4:427098/1‑139 (MQ=17)
                                        cagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcag                                                                                                      <  4:27666/141‑1 (MQ=255)
                                         agatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcaga                                                                                                     <  1:91163/141‑1 (MQ=255)
                                            tAGTGATTCAGCCTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattc                                                                                                  <  1:98002/141‑1 (MQ=255)
                                            tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTAAGACTCAGATAGCGACTCAGACTCAGATAGCgaatcagattc                                                                                                  >  2:1140143/1‑141 (MQ=255)
                                            tAGTGATTCAGACTCAGATAGCGACTCAGAATCAGACAGCGACTCAGAGTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattc                                                                                                  <  1:859377/141‑1 (MQ=255)
                                                gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATtcagattcagat                                                                                              >  1:1076795/1‑141 (MQ=255)
                                                  ttCAGACTCAGATAGCGACTCGGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGCGACTCCGACTCAGATAGCGATTCAGATTCAGATAg                                                                                            >  2:1145440/1‑141 (MQ=255)
                                                  ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                                                                            <  1:766526/141‑1 (MQ=255)
                                                  ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATCCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAACCAGATACAGCTAg                                                                                            >  2:820784/1‑141 (MQ=18)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAGATCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGAGACTCAGACTCAGATAGCGGCTCACACACAGCGAGCGAAACAGATANAGATAGc                                                                                           >  2:894716/1‑141 (MQ=17)
                                                    cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCg                                                                                          >  1:716530/1‑141 (MQ=255)
                                                    cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCg                                                                                          <  1:1001413/141‑1 (MQ=255)
                                                      gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgac                                                                                        <  3:381326/141‑1 (MQ=255)
                                                      gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgac                                                                                        >  1:1007499/1‑141 (MQ=255)
                                                       aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgact                                                                                       >  2:854391/1‑141 (MQ=255)
                                                           agatagcgactcagattcagatagcgactcagattcGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcaga                                                                                   <  4:40285/141‑1 (MQ=255)
                                                           agacagcgactcagattcagacagcgactcagattcGGATAGCGACTCAGATTCAGGCAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcaga                                                                                   >  4:494683/1‑141 (MQ=255)
                                                           aGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcaga                                                                                   <  2:72535/141‑1 (MQ=255)
                                                           aGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATCCAGACTCAGATAGAgactcaga                                                                                   >  4:460769/1‑141 (MQ=255)
                                                            gATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcagac                                                                                  >  3:92170/1‑141 (MQ=255)
                                                            gATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcagac                                                                                  >  2:86898/1‑141 (MQ=255)
                                                              tAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACCCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcagactc                                                                                <  4:165271/141‑1 (MQ=255)
                                                                gcgactcagaatcagatagcgactcagaatcagataGTGAGTCAGATTCAGACAGTGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGactcagactcag                                                                              <  2:576755/141‑1 (MQ=11)
                                                                gCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGactcagactcag                                                                              <  1:20219/141‑1 (MQ=255)
                                                                       aGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGa                                                                       <  4:382785/141‑1 (MQ=255)
                                                                        tatgcAGACAGCGACTCTGCTTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt                                                                      <  2:918695/137‑1 (MQ=255)
                                                                        gATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt                                                                      <  1:519865/141‑1 (MQ=255)
                                                                        gATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt                                                                      <  3:76728/141‑1 (MQ=255)
                                                                        gATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt                                                                      <  2:173261/141‑1 (MQ=255)
                                                                        gATCCAGAAAGCGACTCAGCTTCGAATAGCGACTGAGATTCAGACAGCAACTTAGAATCCGATTGTGACTCAGGCTCAGATAGCGACTCAGAGACAGATAGCGATTCAGAATCAGATAGCGACTCAGACTCAGCAAGCggt                                                                      >  4:116298/1‑139 (MQ=17)
                                                                            cAGACAGCCACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcag                                                                  <  2:1027104/141‑1 (MQ=255)
                                                                              gACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATCGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACtcagac                                                                >  2:1141636/1‑141 (MQ=255)
                                                                              gACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagac                                                                <  2:64050/141‑1 (MQ=255)
                                                                                cAGCGACTCAGACTCGGACCGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATtcagactc                                                              <  2:323876/141‑1 (MQ=14)
                                                                                 aGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactca                                                             >  2:335063/1‑141 (MQ=255)
                                                                                  gCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactcag                                                            >  1:252491/1‑141 (MQ=255)
                                                                                   cGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactcaga                                                           <  3:467488/141‑1 (MQ=255)
                                                                                   cGACTCAGATTCAGACAGCGACTAAGATTCACACAGCGACTCAGACACGAATAGTGACTCAGAATCAGAGAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactcaga                                                           <  2:991328/141‑1 (MQ=14)
                                                                                       tCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGc                                                       >  2:671943/1‑141 (MQ=255)
                                                                                         aGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCga                                                     <  3:228987/141‑1 (MQ=255)
                                                                                          gATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgac                                                    >  2:468081/1‑141 (MQ=255)
                                                                                          gATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAACGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgac                                                    >  4:379762/1‑141 (MQ=255)
                                                                                               ggATAGCGCCTCAGATTTAGCCAGCGACTCAGCCTCCGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcaga                                               <  2:962558/141‑1 (MQ=17)
                                                                                                 aTAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcagact                                             <  2:391897/141‑1 (MQ=255)
                                                                                                  tAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                                                                                                                                >  3:434909/1‑57 (MQ=40)
                                                                                                  tAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcagactc                                            >  1:850047/1‑141 (MQ=255)
                                                                                                     cGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGActcagactcaga                                         >  2:249037/1‑141 (MQ=255)
                                                                                                      gactcagactcaGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACACAGACAGCGAATCAGACTCAGAAAGCGACTCAGACGCAGAAAGCGATGCAGACACAGACAGCGAcgcagacgcagaa                                        >  2:19104/1‑140 (MQ=11)
                                                                                                      gACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGAt                                        <  1:797641/141‑1 (MQ=255)
                                                                                                       aCTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATg                                       <  3:490860/141‑1 (MQ=255)
                                                                                                        cTCAGAGTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGc                                      <  4:423/141‑1 (MQ=255)
                                                                                                            gATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagac                                                                                                                      <  2:356344/57‑1 (MQ=38)
                                                                                                               tCAGACAGCGAGTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAg                               <  4:392551/141‑1 (MQ=255)
                                                                                                               tCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACCCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAg                               >  4:283646/1‑141 (MQ=255)
                                                                                                                  gACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGGTTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCTAGTAAGcac                            >  3:56441/1‑141 (MQ=255)
                                                                                                                    ctgCGACTCACACTCGGATAGTGATTCAGACTCAGAAAGCGACTCACACTCGGAGAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcacac                          <  2:865042/139‑1 (MQ=18)
                                                                                                                    catcGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcacac                          <  4:215888/138‑1 (MQ=255)
                                                                                                                     aGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcacaca                         <  2:406627/141‑1 (MQ=255)
                                                                                                                     aGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcacaca                         <  1:19104/141‑1 (MQ=255)
                                                                                                                     aGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcacaca                         <  2:59517/141‑1 (MQ=255)
                                                                                                                          cTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt                    <  3:460769/141‑1 (MQ=255)
                                                                                                                          cTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt                    <  3:2219/141‑1 (MQ=255)
                                                                                                                          cTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt                    <  1:331320/141‑1 (MQ=255)
                                                                                                                          cTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt                    <  3:49625/141‑1 (MQ=255)
                                                                                                                          cTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt                    <  1:349101/141‑1 (MQ=255)
                                                                                                                            cAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTa                  <  3:283646/141‑1 (MQ=255)
                                                                                                                               aCTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAAc               <  2:530392/141‑1 (MQ=255)
                                                                                                                                 tCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCa             >  2:642749/1‑141 (MQ=255)
                                                                                                                                  cGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCaa            >  1:608352/1‑141 (MQ=255)
                                                                                                                                   ggATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAAt           >  3:165114/1‑141 (MQ=255)
                                                                                                                                    gATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATg          >  2:116233/1‑141 (MQ=255)
                                                                                                                                      tAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCTGACTCAGACATTTATTCAGACTTAGACTGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAg        >  3:300245/1‑141 (MQ=21)
                                                                                                                                          gATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGtact    >  4:202248/1‑141 (MQ=255)
                                                                                                                                          gATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGtact    >  2:1119430/1‑141 (MQ=255)
                                                                                                                                           aTTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGtacta   <  1:151402/141‑1 (MQ=255)
                                                                                                                                           aTTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGtacta   <  1:468081/141‑1 (MQ=255)
                                                                                                                                            ttCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGtactac  >  3:506036/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGTACTAC  >  USA300TCH1516_ALE/618619‑618899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: