Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,464 G→A 11.2% S1009S (TCG→TCA)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4640GA11.2% 377.2 / 8.3 156S1009S (TCG→TCA) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (68/62);  new base A (12/8);  total (82/74)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

GTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622331‑622604
                                                                                                                                     |                                                                                                                                            
gTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                                                                                       >  1:905293/1‑141 (MQ=255)
 tGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                                      <  1:170741/141‑1 (MQ=255)
 tGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                                      <  4:337066/141‑1 (MQ=255)
   attcagattcagACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAACGACTCTGATTCAGATAGCGATTCGGACTCAGACa                                                                                                                                    <  2:950858/141‑1 (MQ=255)
     tcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACGCAGATAGCGGCTCAGACTCAGACAgg                                                                                                                                  >  2:387311/1‑140 (MQ=14)
      cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACACAGACAGCGACTCAGATGCAGAAAGCGATACAGCCGCCGACAGCg                                                                                                                                 >  2:767175/1‑141 (MQ=14)
       agattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGa                                                                                                                                <  1:96858/141‑1 (MQ=255)
          ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactc                                                                                                                             <  1:1188255/141‑4 (MQ=11)
           tcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGAGACTCAGATTCAGATAGCGATTCAGACGCAGACAgagagaca                                                                                                                            >  2:1061828/1‑137 (MQ=11)
            cagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAg                                                                                                                           <  1:755767/141‑1 (MQ=255)
             agaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCGGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGATTCAGa                                                                                                                          >  1:595652/1‑141 (MQ=255)
              gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                         <  4:210906/141‑1 (MQ=255)
                cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc                                                                                                                       <  2:463405/141‑1 (MQ=255)
                  gCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGactcagactcag                                                                                                                     >  2:88784/1‑141 (MQ=255)
                  gCGACACAGATTCAGATAGCGACTCAGACTCCGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAg                                                                                                                     <  2:209556/141‑1 (MQ=255)
                   cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCTATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                    <  1:1108540/141‑1 (MQ=255)
                   cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                    <  2:506568/141‑1 (MQ=255)
                      cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                 <  2:982069/141‑1 (MQ=255)
                       tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                >  1:826155/1‑141 (MQ=255)
                       tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCGGACTCAGACAGCGAGTCAGACTCAGATAGc                                                                                                                >  2:1044799/1‑141 (MQ=255)
                       tcagattcagatAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCTGATAGc                                                                                                                >  2:487024/1‑141 (MQ=255)
                        cagattcagatAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGAGACTCAGATTCAGAAAGCGATACAGACGCAGAAAGCGAGTCAGACACAGAAAGcc                                                                                                               >  2:910409/1‑140 (MQ=11)
                          gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                             <  1:144129/141‑1 (MQ=255)
                          gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                             >  3:133613/1‑141 (MQ=255)
                            ttcagatAGCGACTGAGATTCGGAGGGCGATTCAGGCGCAGATGGCGACTTAGATTCAGAGAGCGATGCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                           <  4:247612/141‑1 (MQ=14)
                            ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAgtagtgt                                                                                                           >  4:455395/1‑135 (MQ=255)
                              cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                         >  1:1065127/1‑141 (MQ=255)
                              cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                         >  3:417985/1‑141 (MQ=255)
                               agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                                                                        <  2:22090/141‑1 (MQ=255)
                                gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                       >  2:400315/1‑141 (MQ=255)
                                gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACAGAGACAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGGACGCGAGTCAGACGCAGCGAGAgacgcaggc                                                                                                       >  2:400334/1‑139 (MQ=11)
                                     cGACTCAGATTCGTACAGCGATTCACACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGAttcagactcaga                                                                                                  <  2:11963/141‑1 (MQ=255)
                                      gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                 <  1:1137425/141‑1 (MQ=255)
                                      gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                 <  1:601783/141‑1 (MQ=255)
                                        cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                                               >  3:87317/1‑141 (MQ=255)
                                         tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                                                                              >  4:455815/1‑141 (MQ=255)
                                           aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                            <  1:764735/141‑1 (MQ=255)
                                            gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           >  4:29399/1‑141 (MQ=255)
                                              ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                         >  2:110801/1‑141 (MQ=255)
                                              ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                         <  1:404810/141‑1 (MQ=255)
                                                cagataGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                       >  1:121215/6‑141 (MQ=11)
                                                cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                       >  2:778804/1‑141 (MQ=255)
                                                    cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCAGCGGACGCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagagtc                                                                                   >  1:21054/1‑141 (MQ=14)
                                                      gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                 >  1:556580/1‑141 (MQ=255)
                                                      gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                 >  2:326109/1‑141 (MQ=255)
                                                      gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCGGACTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                 >  3:317672/1‑141 (MQ=255)
                                                       cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                                                                                >  4:281606/1‑141 (MQ=255)
                                                              gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                         >  1:1085104/1‑141 (MQ=37)
                                                                ttCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                       >  4:132884/2‑141 (MQ=255)
                                                                cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                       >  3:274961/1‑141 (MQ=255)
                                                                cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGaacc                                                                       >  4:330804/1‑138 (MQ=255)
                                                                  cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGTGATTCGGATTCAGCGAGTGATTCgg                                                                     <  2:1124207/141‑1 (MQ=11)
                                                                   aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                    >  3:264216/1‑141 (MQ=255)
                                                                    gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                   <  1:1063237/141‑1 (MQ=255)
                                                                    gATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                   <  1:1133533/141‑1 (MQ=255)
                                                                      tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                 <  1:334841/141‑1 (MQ=255)
                                                                      tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                 >  1:648331/1‑141 (MQ=255)
                                                                      aaGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                 <  2:12511/140‑1 (MQ=255)
                                                                       aGCGACTCAGATTCAGATAGCGCCTCAGCCGCAGACAGCGACTCAGATTCAGACAGCGATTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGATACAGAGAGCGACTCGGACTCa                                                                >  2:542608/1‑141 (MQ=14)
                                                                         cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                              <  4:131885/141‑1 (MQ=255)
                                                                            cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                           >  3:204424/1‑141 (MQ=255)
                                                                            cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                           <  1:1087574/141‑1 (MQ=255)
                                                                            cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAg                                                           >  4:503742/1‑141 (MQ=255)
                                                                            cTCAGATTCAGATAGCGAGCCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                           <  2:441410/141‑1 (MQ=255)
                                                                               agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGa                                                        >  4:439290/1‑141 (MQ=255)
                                                                                gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                       >  1:421084/1‑141 (MQ=255)
                                                                                  ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                     >  1:1175523/1‑141 (MQ=255)
                                                                                   tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                    >  1:86064/1‑141 (MQ=255)
                                                                                    cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                   <  1:1162300/141‑1 (MQ=255)
                                                                                    cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                   >  3:472261/1‑141 (MQ=255)
                                                                                    cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                   >  3:472235/1‑141 (MQ=255)
                                                                                     agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGa                                                  >  1:935597/1‑141 (MQ=255)
                                                                                      gatAGCGATTCAGACTCAGACGGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCGGACTCAGAGAGCGACTCAGaa                                                 >  2:438781/1‑140 (MQ=255)
                                                                                        tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                               >  1:571376/1‑141 (MQ=255)
                                                                                        tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                               >  3:57744/1‑141 (MQ=255)
                                                                                        tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTc                                               >  2:1032195/1‑141 (MQ=255)
                                                                                        tAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGacac                                               >  2:758884/1‑138 (MQ=11)
                                                                                              ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                         <  1:3956/141‑1 (MQ=255)
                                                                                                  gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                     <  3:504906/141‑1 (MQ=255)
                                                                                                  gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                     >  1:130582/1‑141 (MQ=255)
                                                                                                     tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                  >  2:268207/1‑141 (MQ=255)
                                                                                                      cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                 <  3:205777/141‑1 (MQ=255)
                                                                                                      cagacAGCGACTAAGATTCAGATAGCGATACAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                 <  2:1093131/141‑1 (MQ=255)
                                                                                                       agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGAAAGCGACTCAGa                                >  2:1141525/1‑141 (MQ=11)
                                                                                                       agacAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                <  2:811025/141‑1 (MQ=255)
                                                                                                        gacAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                               <  2:694147/141‑1 (MQ=255)
                                                                                                           aGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                            >  3:420315/1‑141 (MQ=255)
                                                                                                            gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                           >  1:545944/1‑141 (MQ=255)
                                                                                                            gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                           >  2:513621/1‑141 (MQ=255)
                                                                                                              gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                         >  1:316017/1‑141 (MQ=255)
                                                                                                              gACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                         <  2:158939/141‑1 (MQ=255)
                                                                                                                 tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                         <  2:583061/90‑1 (MQ=255)
                                                                                                                    gattcagagAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                   <  1:1019137/141‑1 (MQ=255)
                                                                                                                      ttcagacAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                 <  1:1007668/141‑1 (MQ=255)
                                                                                                                            tAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCTGATAGCGATTCGGACTCAGATAGCGACTCAGATTcagatagcgactcagactcagatagcgactcagactt           >  4:289831/1‑140 (MQ=14)
                                                                                                                                  ttCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAGTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg     <  4:276691/141‑1 (MQ=255)
                                                                                                                                  ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg     <  2:826155/141‑1 (MQ=255)
                                                                                                                                   tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt    >  1:354289/1‑141 (MQ=255)
                                                                                                                                     ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga  <  2:556580/141‑1 (MQ=255)
                                                                                                                                     ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAATCAGACAGCGATTCAGATTCAGACAgcga  <  4:140506/141‑4 (MQ=11)
                                                                                                                                     |                                                                                                                                            
GTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622331‑622604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑