Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,479 T→C 6.5% D1014D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4790TC 6.5% 408.8 / 3.5 159D1014D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (68/74);  new base C (11/2);  total (82/77)
Fisher's exact test for biased strand distribution p-value = 1.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

CAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGA  >  USA300TCH1516_ALE/622343‑622616
                                                                                                                                        |                                                                                                                                         
cagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAg                                                                                                                                       <  1:755767/141‑1 (MQ=255)
cagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAg                                                                                                                                       >  1:476483/1‑141 (MQ=255)
  gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                                     <  4:210906/141‑1 (MQ=255)
      gCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGactcagactcag                                                                                                                                 >  2:88784/1‑141 (MQ=255)
      gCGACACAGATTCAGATAGCGACTCAGACTCCGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAg                                                                                                                                 <  2:209556/141‑1 (MQ=255)
       cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                                <  2:506568/141‑1 (MQ=255)
          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGACAGCGATTCAGACTCAGATAg                                                                                                                             >  1:627566/1‑141 (MQ=255)
          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                             <  2:982069/141‑1 (MQ=255)
          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                             <  3:87719/141‑1 (MQ=255)
           tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                            >  1:826155/1‑141 (MQ=255)
           tcagattcagatAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCTGATAGc                                                                                                                            >  2:487024/1‑141 (MQ=255)
              gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                         <  1:144129/141‑1 (MQ=255)
                ttcagatAGCGACTGAGATTCGGAGGGCGATTCAGGCGCAGATGGCGACTTAGATTCAGAGAGCGATGCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                       <  4:247612/141‑1 (MQ=14)
                ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAgtagtgt                                                                                                                       >  4:455395/1‑135 (MQ=255)
                  cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                                     >  3:417985/1‑141 (MQ=255)
                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                                   >  2:400315/1‑141 (MQ=255)
                         cGACTCAGATTCGTACAGCGATTCACACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGAttcagactcaga                                                                                                              <  2:11963/141‑1 (MQ=255)
                          gtcTCAGAATCAGATAGCGATTCAGAATCAGAGAGCGACTCAGATCCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                             <  2:448289/139‑1 (MQ=11)
                          gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                             <  1:601783/141‑1 (MQ=255)
                            atCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                                                           <  2:389719/140‑1 (MQ=255)
                             tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                                                                                          >  4:455815/1‑141 (MQ=255)
                               aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                                        <  1:764735/141‑1 (MQ=255)
                                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                       >  4:29399/1‑141 (MQ=255)
                                  ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                                     <  1:404810/141‑1 (MQ=255)
                                  tgcGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                                     <  1:1077592/139‑1 (MQ=255)
                                    cagataGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                   >  1:121215/6‑141 (MQ=11)
                                    cagACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                   <  2:82402/139‑1 (MQ=255)
                                    cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                   <  1:1051770/141‑1 (MQ=255)
                                    cGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                   <  4:255107/141‑1 (MQ=255)
                                      gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                                 >  1:1123107/1‑141 (MQ=255)
                                      gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGC‑‑‑‑‑‑GACTCAGACAGCGACTCAGACTCAGATAGAGATGCAGACTCAGACAGCGACacagactcagac                                                                                           >  2:777602/1‑140 (MQ=11)
                                        cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCAGCGGACGCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagagtc                                                                                               >  1:21054/1‑141 (MQ=14)
                                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                             >  1:556580/1‑141 (MQ=255)
                                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                             >  2:326109/1‑141 (MQ=255)
                                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCGGACTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                             >  3:317672/1‑141 (MQ=255)
                                           cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                                                                                            >  4:281606/1‑141 (MQ=255)
                                               tcagactcagacAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                        <  2:1176409/141‑1 (MQ=255)
                                               tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                        >  2:436237/1‑141 (MQ=255)
                                               tCAGACTCAGATAGCGACTCAGATGCAGATAGCGATTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGATTCGGACTCAGCCAGCGATTCCGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                        <  1:979579/141‑1 (MQ=255)
                                                cAGACTCAGATAGCGACTCAGATCCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCg                                                                                       >  2:1108725/1‑141 (MQ=255)
                                                    cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                                   >  3:274961/1‑141 (MQ=255)
                                                    cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGaacc                                                                                   >  4:330804/1‑138 (MQ=255)
                                                      cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGTGATTCGGATTCAGCGAGTGATTCgg                                                                                 <  2:1124207/141‑1 (MQ=11)
                                                       aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCAGa                                                                                >  2:741951/1‑141 (MQ=11)
                                                        gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                               <  1:1063237/141‑1 (MQ=255)
                                                        gATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                               <  1:1133533/141‑1 (MQ=255)
                                                          tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                             <  1:334841/141‑1 (MQ=255)
                                                           aGCGACTCAGATTCAGATAGCGCCTCAGCCGCAGACAGCGACTCAGATTCAGACAGCGATTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGATACAGAGAGCGACTCGGACTCa                                                                            >  2:542608/1‑141 (MQ=14)
                                                             cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                          <  3:60972/141‑1 (MQ=255)
                                                             cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                          <  1:404501/141‑1 (MQ=255)
                                                             cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                          <  4:131885/141‑1 (MQ=255)
                                                              gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                         <  1:906299/141‑1 (MQ=255)
                                                               aCTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATa                                                                        <  2:127774/141‑1 (MQ=255)
                                                                cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAGAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACGCAGATAg                                                                       >  4:134213/1‑141 (MQ=255)
                                                                cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                       >  3:204424/1‑141 (MQ=255)
                                                                cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                       <  1:1087574/141‑1 (MQ=255)
                                                                cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAg                                                                       >  4:503742/1‑141 (MQ=255)
                                                                  cagattcagacAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                     <  2:846342/141‑1 (MQ=255)
                                                                   agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                    <  2:240181/141‑1 (MQ=255)
                                                                   agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGa                                                                    >  4:439290/1‑141 (MQ=255)
                                                                      ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                 >  1:1175523/1‑141 (MQ=255)
                                                                       tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                >  1:86064/1‑141 (MQ=255)
                                                                        cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                               >  3:472235/1‑141 (MQ=255)
                                                                          gatAGCGATTCAGACTCAGACGGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCGGACTCAGAGAGCGACTCAGaa                                                             >  2:438781/1‑140 (MQ=255)
                                                                            tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                           >  1:1160449/1‑141 (MQ=255)
                                                                            tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                           >  2:1032195/1‑141 (MQ=255)
                                                                            tAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGacac                                                           >  2:758884/1‑138 (MQ=11)
                                                                              gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCTGTTTCAGACAGAGATTAAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCTGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAg                                                         <  4:28922/141‑1 (MQ=11)
                                                                                  cacagactcagtcTGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCTATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                     <  2:174752/139‑1 (MQ=255)
                                                                                      gatgaagacAGCGAACCAGATACAGATAGCGATGCAGACGATGCAAGAGACTGAGATTCAGACACAGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                 <  1:1103461/136‑1 (MQ=2)
                                                                                      gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                 >  1:130582/1‑141 (MQ=255)
                                                                                      gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                 <  3:504906/141‑1 (MQ=255)
                                                                                      gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                 >  3:243498/1‑141 (MQ=255)
                                                                                        ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                               >  1:1185325/1‑141 (MQ=255)
                                                                                         tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                              >  2:268207/1‑141 (MQ=255)
                                                                                          cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                             <  3:48698/141‑1 (MQ=255)
                                                                                           agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                            <  3:275468/141‑1 (MQ=255)
                                                                                           agacAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                            <  2:811025/141‑1 (MQ=255)
                                                                                            gtcAGCGACTCAGATTCAGATAGCGATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                           <  2:1082061/139‑1 (MQ=255)
                                                                                            gacAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                           <  2:694147/141‑1 (MQ=255)
                                                                                                gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                       >  1:545944/1‑141 (MQ=255)
                                                                                                gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                       >  2:513621/1‑141 (MQ=255)
                                                                                                  gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                     >  1:316017/1‑141 (MQ=255)
                                                                                                     tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                     >  1:583061/1‑90 (MQ=255)
                                                                                                      cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCGGATAGCg                                 <  2:648331/141‑1 (MQ=255)
                                                                                                        gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                               >  2:1052521/1‑141 (MQ=255)
                                                                                                        gattcagagAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                               <  1:1019137/141‑1 (MQ=255)
                                                                                                          ttcagacAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                             <  1:1007668/141‑1 (MQ=255)
                                                                                                           tcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATATCgactca                            <  3:445188/141‑1 (MQ=255)
                                                                                                                       tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt                >  1:354289/1‑141 (MQ=255)
                                                                                                                        cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg               <  1:694789/141‑1 (MQ=255)
                                                                                                                         ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga              <  3:132884/141‑1 (MQ=255)
                                                                                                                         ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga              <  2:556580/141‑1 (MQ=255)
                                                                                                                         ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAATCAGACAGCGATTCAGATTCAGACAgcga              <  4:140506/141‑4 (MQ=11)
                                                                                                                          gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat             >  3:165592/1‑141 (MQ=255)
                                                                                                                              caggaaGCGGTTCAGATTCAGACAGTGACTGAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGAGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag         <  2:842666/136‑1 (MQ=14)
                                                                                                                              cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag         >  3:330526/1‑141 (MQ=255)
                                                                                                                                gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat       >  1:206520/1‑141 (MQ=255)
                                                                                                                                   aGCGAACCAGACTCCGATAGCCGCCCCGCCTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattca    <  2:1096079/141‑1 (MQ=17)
                                                                                                                                     cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCGGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga  <  1:591466/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGA  >  USA300TCH1516_ALE/622343‑622616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑