Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,521 T→C 6.2% D1028D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5210TC 6.2% 379.5 / 3.9 136D1028D (GAT→GACsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (61/63);  new base C (8/2);  total (70/66)
Fisher's exact test for biased strand distribution p-value = 9.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTC  >  USA300TCH1516_ALE/622381‑622661
                                                                                                                                            |                                                                                                                                            
gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                                                                              >  1:1123107/1‑141 (MQ=255)
gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGC‑‑‑‑‑‑GACTCAGACAGCGACTCAGACTCAGATAGAGATGCAGACTCAGACAGCGACacagactcagac                                                                                                                                        >  2:777602/1‑140 (MQ=11)
  cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCAGCGGACGCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagagtc                                                                                                                                            >  1:21054/1‑141 (MQ=14)
    gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                                                                          >  1:556580/1‑141 (MQ=255)
    gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                                                                          >  2:326109/1‑141 (MQ=255)
    gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCGGACTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                                                                          >  3:317672/1‑141 (MQ=255)
     cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                                                                                                                                         >  4:281606/1‑141 (MQ=255)
         tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                                                                     >  2:436237/1‑141 (MQ=255)
         tCAGACTCAGATAGCGACTCAGATGCAGATAGCGATTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGATTCGGACTCAGCCAGCGATTCCGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                                                                     <  1:979579/141‑1 (MQ=255)
          cAGACTCAGATAGCGACTCAGATCCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCg                                                                                                                                    >  2:1108725/1‑141 (MQ=255)
            gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                                                                  >  1:1085104/1‑141 (MQ=37)
              cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                                                                                >  3:274961/1‑141 (MQ=255)
                 aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                             >  3:264216/1‑141 (MQ=255)
                 aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCAGa                                                                                                                             >  2:741951/1‑141 (MQ=11)
                  gATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                            <  1:1133533/141‑1 (MQ=255)
                    tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                          <  1:334841/141‑1 (MQ=255)
                    tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                          >  1:648331/1‑141 (MQ=255)
                    aaGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                          <  2:12511/140‑1 (MQ=255)
                     aGCGACTCAGATTCAGATAGCGCCTCAGCCGCAGACAGCGACTCAGATTCAGACAGCGATTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGATACAGAGAGCGACTCGGACTCa                                                                                                                         >  2:542608/1‑141 (MQ=14)
                       cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                       <  1:404501/141‑1 (MQ=255)
                       cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                       <  3:60972/141‑1 (MQ=255)
                       cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                       <  4:131885/141‑1 (MQ=255)
                        gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                      <  1:906299/141‑1 (MQ=255)
                         aCTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATa                                                                                                                     <  2:127774/141‑1 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAGAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACGCAGATAg                                                                                                                    >  4:134213/1‑141 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    >  3:204424/1‑141 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    <  1:1087574/141‑1 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    >  1:820356/1‑141 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAg                                                                                                                    >  4:503742/1‑141 (MQ=255)
                            cagattcagacAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                  <  2:846342/141‑1 (MQ=255)
                             agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                 >  4:439290/1‑141 (MQ=255)
                              gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                >  1:421084/1‑141 (MQ=255)
                                 tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                                                             >  1:86064/1‑141 (MQ=255)
                                  cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                            <  1:1162300/141‑1 (MQ=255)
                                  cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                            >  3:472261/1‑141 (MQ=255)
                                    gatAGCGATTCAGACTCAGACGGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCGGACTCAGAGAGCGACTCAGaa                                                                                                          >  2:438781/1‑140 (MQ=255)
                                      tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                        >  3:57744/1‑141 (MQ=255)
                                      tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                        >  1:571376/1‑141 (MQ=255)
                                      tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                        >  1:1160449/1‑141 (MQ=255)
                                        gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCTGTTTCAGACAGAGATTAAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCTGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAg                                                                                                      <  4:28922/141‑1 (MQ=11)
                                            ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                  <  1:3956/141‑1 (MQ=255)
                                            cacagactcagtcTGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCTATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                  <  2:174752/139‑1 (MQ=255)
                                                gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                              >  1:130582/1‑141 (MQ=255)
                                                  ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                            >  1:1185325/1‑141 (MQ=255)
                                                   tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                           >  2:268207/1‑141 (MQ=255)
                                                    cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                          <  3:48698/141‑1 (MQ=255)
                                                    cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                          <  3:205777/141‑1 (MQ=255)
                                                     agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                         <  3:275468/141‑1 (MQ=255)
                                                     agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGAAAGCGACTCAGa                                                                                         >  2:1141525/1‑141 (MQ=11)
                                                      gtcAGCGACTCAGATTCAGATAGCGATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                        <  2:1082061/139‑1 (MQ=255)
                                                         aGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                                     >  3:420315/1‑141 (MQ=255)
                                                          gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                                    >  1:545944/1‑141 (MQ=255)
                                                          gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                    >  2:513621/1‑141 (MQ=255)
                                                            gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                  >  1:316017/1‑141 (MQ=255)
                                                               tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                                                                  >  1:583061/1‑90 (MQ=255)
                                                                cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCGGATAGCg                                                                              <  2:648331/141‑1 (MQ=255)
                                                                  gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                            >  2:1052521/1‑141 (MQ=255)
                                                                  gattcagagAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                            <  1:1019137/141‑1 (MQ=255)
                                                                    ttcagacAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                          <  1:1007668/141‑1 (MQ=255)
                                                                     tcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATATCgactca                                                                         <  3:445188/141‑1 (MQ=255)
                                                                       agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGATTCAGATAGCGATTCAGACTCAGATAGAGACTCAGATTCAGACAGCGAATCAGAATCAGATATCgactcaga                                                                       >  4:212952/1‑141 (MQ=14)
                                                                                ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                              <  2:826155/141‑1 (MQ=255)
                                                                                   ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                           <  2:556580/141‑1 (MQ=255)
                                                                                   ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                           <  3:132884/141‑1 (MQ=255)
                                                                                   ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAATCAGACAGCGATTCAGATTCAGACAgcga                                                           <  4:140506/141‑4 (MQ=11)
                                                                                      cccAGAGACCGACTCCGACTCAGACAGCGACCCAGACCCGGACAGCGGCCCAGATTCAGATAGCGACTCGGACTCAGAGAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattc                                                        <  2:857224/139‑1 (MQ=14)
                                                                                         aGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                     >  1:752639/1‑141 (MQ=255)
                                                                                         aGACAGCGATTCAGACTCAGATAGCGACCAAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCgattcaga                                                     <  2:762851/141‑1 (MQ=255)
                                                                                          gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat                                                    >  1:206520/1‑141 (MQ=255)
                                                                                            cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgcttcagattc                                                  <  1:1047172/141‑1 (MQ=255)
                                                                                            cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                  >  2:321903/1‑141 (MQ=255)
                                                                                            cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                  >  2:728417/1‑141 (MQ=255)
                                                                                             aGCGAACCAGACTCCGATAGCCGCCCCGCCTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattca                                                 <  2:1096079/141‑1 (MQ=17)
                                                                                               cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCGGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga                                               <  1:591466/141‑1 (MQ=255)
                                                                                                    cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCg                                          >  4:315777/1‑141 (MQ=255)
                                                                                                    cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCCGAAAgag                                          >  2:102455/1‑139 (MQ=14)
                                                                                                      gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgac                                        >  1:430692/1‑141 (MQ=255)
                                                                                                          cAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcag                                    <  1:1052521/141‑1 (MQ=255)
                                                                                                           cgacaGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcaga                                   <  4:303670/137‑1 (MQ=255)
                                                                                                              tAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagattc                                >  4:252591/1‑141 (MQ=255)
                                                                                                                gCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGactcagactcag                              >  3:314199/1‑141 (MQ=255)
                                                                                                                gCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGAGactcagactcag                              >  4:485936/1‑141 (MQ=255)
                                                                                                                  gactcagactcaGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGAt                            >  1:445573/1‑141 (MQ=255)
                                                                                                                    ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAGAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGActcagactcagacaa                          >  2:966909/1‑138 (MQ=255)
                                                                                                                      cagactcagacAGCGACTCAGATTCAGATATCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCg                        <  3:85622/141‑1 (MQ=255)
                                                                                                                      cagacgcagacAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCg                        <  2:459523/141‑1 (MQ=14)
                                                                                                                       tgacacagatAGCGCCTCAGATTCAGAAAGCGACTCAGACTCAGACACAGACTGAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGa                       <  4:36402/140‑1 (MQ=14)
                                                                                                                        gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGAc                      <  1:907613/141‑1 (MQ=255)
                                                                                                                            cagacACCGACTCAGAGGCAGACAGCGATGGTGACGCAGATTGCGACGCTGACGCAGACAGCGTATCAGACTCAGATGGCGAGTCTGACTCAGACAGTGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAg                  <  2:11518/141‑1 (MQ=9)
                                                                                                                              gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAt                >  1:52734/1‑141 (MQ=255)
                                                                                                                                ctgCGACTCAGATTCAGACACCGATAGTGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc              <  2:83030/139‑1 (MQ=17)
                                                                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACCGCGATTCAGACTCAGATAGCGAATCAGACCCAGACAGTGACTCAGATACAGCCAGCGACTCAGACTCAGAGAGCGACCCAGAtcc              >  2:630106/1‑139 (MQ=14)
                                                                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga                                               >  2:405229/1‑108 (MQ=255)
                                                                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc              >  2:277986/1‑141 (MQ=255)
                                                                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc              >  2:165659/1‑141 (MQ=255)
                                                                                                                                    gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc          >  2:577094/1‑141 (MQ=255)
                                                                                                                                    gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc          >  1:76816/1‑141 (MQ=255)
                                                                                                                                      cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAg        <  2:445573/141‑1 (MQ=255)
                                                                                                                                          gattcagcaTGCGATACCGACTCAGACAGAGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGAc    <  1:102607/141‑1 (MQ=17)
                                                                                                                                            ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTc  <  1:438781/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTC  >  USA300TCH1516_ALE/622381‑622661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: