BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	691,587	G→T	7.3%	Q79K (CAA→AAA)	USA300HOU_RS03335 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	691,587	0	G	T	7.3%	470.9 / 7.7	143	Q79K (CAA→AAA)	USA300HOU_RS03335	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (66/64); new base T (10/2); total (77/66)
Fisher's exact test for biased strand distribution p-value = 3.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

TCAACAATTCTTTTTCTCTAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGATTTTTA  >  USA300TCH1516_ALE/691449‑691726

     aaTTCTTTTTCTCTAGATTCCAATACGTTAAAATCCTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCata                                                                                                                                      >  2:355707/1‑141 (MQ=255)

       ttCTTTTTCTCTAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATAtt                                                                                                                                    <  4:501030/141‑1 (MQ=255)

       ttCTTTTTCTCTAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATAtt                                                                                                                                    <  1:591721/141‑1 (MQ=255)

         cTTTTTCTCTAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCg                                                                                                                                  <  2:391294/141‑1 (MQ=255)

         cTTTTTCTCTAGATTCCAATTCTTTAGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCg                                                                                                                                  <  3:349579/141‑1 (MQ=255)

          tttttCTCTAGATTCCAATTCTTTTGAATCTTTAGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGt                                                                                                                                 <  4:380682/141‑1 (MQ=255)

            tttCTCTAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTTAATCATATTCGttt                                                                                                                               <  2:367557/141‑1 (MQ=255)

               ctctAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTttat                                                                                                                            <  2:869308/141‑1 (MQ=255)

                      ttCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGc                                                                                                                     <  1:81942/141‑1 (MQ=255)

                      ttCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGc                                                                                                                     <  2:798359/141‑1 (MQ=255)

                        ccAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGctt                                                                                                                   <  3:2399/141‑1 (MQ=255)

                         cAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGcttc                                                                                                                  >  1:1004721/1‑141 (MQ=255)

                           aTTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGcttctt                                                                                                                >  1:60545/1‑141 (MQ=255)

                           aTTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGCTTTATTAACTGcttctt                                                                                                                >  2:411007/1‑141 (MQ=255)

                            ttCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCttt                                                                                                               <  4:457948/141‑1 (MQ=255)

                              cTTTTGAATCTTTCGAGATCTTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTTCTTCTTTAc                                                                                                             >  3:178053/1‑141 (MQ=255)

                               ttttGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTGATTTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTAAATCATATTTGTCTTATTAAGTGCTTCTTTTCt                                                                                                            >  4:35802/1‑141 (MQ=255)

                                tttGACTCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTg                                                                                                           <  4:409089/141‑1 (MQ=255)

                                tttGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTg                                                                                                           <  2:239416/141‑1 (MQ=255)

                                 ttGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGt                                                                                                          <  1:770039/141‑1 (MQ=255)

                                   gAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCa                                                                                                        >  1:1088211/1‑141 (MQ=255)

                                    aaTCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCat                                                                                                       <  2:262721/141‑1 (MQ=255)

                                                 atTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGt                                                                                          <  1:760031/141‑1 (MQ=255)

                                                 atTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGt                                                                                          >  1:896898/1‑141 (MQ=255)

                                                 atTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGt                                                                                          <  1:138475/141‑1 (MQ=255)

                                                  ttCATATACGTATCAATATACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTa                                                                                         <  3:393172/141‑1 (MQ=255)

                                                  ttCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTa                                                                                         <  4:70492/141‑1 (MQ=255)

                                                   tCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAg                                                                                        <  1:138515/141‑1 (MQ=255)

                                                     atatACGTATCAATAAACTTATCAGCATGTAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCt                                                                                      <  2:866250/141‑1 (MQ=255)

                                                     atatACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCt                                                                                      <  2:906371/141‑1 (MQ=255)

                                                     atatACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCt                                                                                      <  2:635232/141‑1 (MQ=255)

                                                          cGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTtaat                                                                                 >  1:180941/1‑141 (MQ=255)

                                                              tCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATaaaa                                                                             <  1:537378/141‑1 (MQ=255)

                                                                  tAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAaagaag                                                                         <  3:332005/141‑1 (MQ=255)

                                                                  tAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAaagaag                                                                         <  2:458080/141‑1 (MQ=255)

                                                                  tAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAaagaag                                                                         <  1:921784/141‑1 (MQ=255)

                                                                  tAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAaagaag                                                                         <  1:896758/141‑1 (MQ=255)

                                                                  tAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAaagaaa                                                                         >  2:984256/1‑140 (MQ=255)

                                                                    aaCTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCt                                                                       >  2:77687/1‑141 (MQ=255)

                                                                    aaCTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCt                                                                       <  2:1093596/141‑1 (MQ=255)

                                                                    aaCTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTCACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCt                                                                       <  2:1024797/141‑1 (MQ=255)

                                                                     aCTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCta                                                                      <  2:840291/141‑1 (MQ=255)

                                                                      cttggcAGCATATAACTTTAATGTAGTACGATACTGAACGTGAGTCGCAGTAGCGGCATACTTTATTTGAATCAGAGTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCtaa                                                                     <  1:950387/136‑1 (MQ=255)

                                                                         aTCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCtaataa                                                                  >  2:922720/1‑141 (MQ=255)

                                                                          tCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATaaa                                                                 >  1:821361/1‑141 (MQ=255)

                                                                          tCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATaaa                                                                 >  2:910357/1‑141 (MQ=255)

                                                                            aGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTTTTTTTTTTTCTTTTTATTAACTGCTTCTTTATTGTTATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATaaaaa                                                               >  4:451837/1‑140 (MQ=255)

                                                                              cATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGaa                                                             >  1:754217/1‑141 (MQ=255)

                                                                              cATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGaa                                                             >  1:44333/1‑141 (MQ=255)

                                                                                tataACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTTTTTCTTATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAAtt                                                           >  2:359896/1‑141 (MQ=255)

                                                                                tataACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAAtt                                                           <  3:105526/141‑1 (MQ=255)

                                                                                   aaCTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAAc                                                        <  2:91310/141‑1 (MQ=255)

                                                                                   aaCTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAAc                                                        <  4:456797/141‑1 (MQ=255)

                                                                                         aaTTTAGGACTATACTGAACTGTATTCGCATTATCTTCATCCTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATa                                                  <  3:348168/141‑1 (MQ=255)

                                                                                         aaTTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATa                                                  <  3:247702/141‑1 (MQ=255)

                                                                                          aTTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACCGCTTCTTTATTGTCACTAATTGCTGTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAg                                                 >  1:350509/1‑141 (MQ=255)

                                                                                            ttAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTTTTTTTTTTTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATATAAGAAGCTAATAAAAGAATTAACAAAATAGCt                                               >  2:495546/1‑141 (MQ=255)

                                                                                            ttAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCt                                               <  1:1065101/141‑1 (MQ=255)

                                                                                              aGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCtaa                                             >  1:683797/1‑141 (MQ=255)

                                                                                               ggACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCtaat                                            >  1:146802/1‑141 (MQ=255)

                                                                                               ggACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCtaat                                            >  1:677763/1‑141 (MQ=255)

                                                                                               ggACTATACTGAACTGTATTCGCATTATCTTAATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATATCtaat                                            >  2:1061580/1‑141 (MQ=255)

                                                                                               ggACTATACTGAACAGTATTCGCATTATCTTAATACTTTTTTTGAATCATATTCGCTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTATTTAAAGAAGCTAATAAAAGAATTAACTAAATCGTtaaa                                            >  2:1061341/1‑140 (MQ=255)

                                                                                                   tataCTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATg                                        >  2:237117/1‑141 (MQ=255)

                                                                                                    ataCTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCGTTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGa                                       >  1:129336/1‑141 (MQ=255)

                                                                                                          aaCTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTaaaa                                 >  2:704370/1‑141 (MQ=255)

                                                                                                            cTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAAc                               >  1:375588/1‑141 (MQ=255)

                                                                                                            cTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAAc                               >  3:439875/1‑141 (MQ=255)

                                                                                                             tGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACa                              <  2:1654/141‑1 (MQ=255)

                                                                                                              gTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACaa                             >  3:205950/1‑141 (MQ=255)

                                                                                                               tATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACaaa                            >  1:864136/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTACTAATGAGTAAAAACAAAtt                          >  2:638198/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAAtt                          >  4:336396/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAAtt                          >  3:208852/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAAtt                          >  2:977872/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAAtt                          >  3:372437/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAAtt                          >  3:41020/1‑141 (MQ=255)

                                                                                                                 ttCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTAACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAAtt                          <  2:850692/141‑1 (MQ=255)

                                                                                                                   cGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGTTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTAc                        >  1:467160/1‑141 (MQ=255)

                                                                                                                   cGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTAc                        <  4:244172/141‑1 (MQ=255)

                                                                                                                   cGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTAc                        <  4:244118/141‑1 (MQ=255)

                                                                                                                   cGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTAc                        >  2:876829/1‑141 (MQ=255)

                                                                                                                   cGCATTATCTTCATACTTTTTTTGAATCATATTCGTGTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAATAATTAACAAAATAGCTAATAATGAGTAAAACCAAATCAc                        >  1:1144452/1‑141 (MQ=255)

                                                                                                                        tATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAatca                   >  3:371774/1‑141 (MQ=255)

                                                                                                                          tCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAatcatt                 >  2:1182533/1‑140 (MQ=255)

                                                                                                                             tCATACTTTTTTTTTTTTTTTTTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTa              >  2:765500/1‑141 (MQ=255)

                                                                                                                             tCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTa              >  1:172372/1‑141 (MQ=255)

                                                                                                                             acATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGATAAAAAAAAAAAAAAACAAATTACAATCATCTTa              <  2:701646/140‑1 (MQ=255)

                                                                                                                              cATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTac             <  2:816357/141‑1 (MQ=255)

                                                                                                                               aTACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTacg            <  4:178053/141‑1 (MQ=255)

                                                                                                                                 aCTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAGTAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTacgac          <  1:998739/141‑1 (MQ=255)

                                                                                                                                  cTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTacgacg         >  1:367725/1‑141 (MQ=255)

                                                                                                                                  cTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTacgacg         <  3:187589/141‑1 (MQ=255)

                                                                                                                                     tttttGAATCATATTCGTTTTATAAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTGAATAAAAGAAACTAATCAAAGAATTAACGAAATAGCTAATAATGCGTAAAAACAAATTAGAATGATCTTACCACGAtt      >  1:354895/1‑141 (MQ=255)

                                                                                                                                      ttttGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGAttt     >  2:1135240/1‑141 (MQ=255)

                                                                                                                                      ttttGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGAttt     <  1:846408/141‑1 (MQ=255)

                                                                                                                                      ttttGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGAttt     >  2:1033968/1‑141 (MQ=255)

                                                                                                                                       tttGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGAtttt    <  3:492430/141‑1 (MQ=255)

                                                                                                                                       tttGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGAtttt    <  2:70621/141‑1 (MQ=255)

                                                                                                                                         tGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGATTTTTa  >  2:177983/1‑141 (MQ=255)

TCAACAATTCTTTTTCTCTAGATTCCAATTCTTTTGAATCTTTCGAGATATTCATATACGTATCAATAAACTTATCAGCATATAACTTTAATTTAGGACTATACTGAACTGTATTCGCATTATCTTCATACTTTTTTTGAATCATATTCGTTTTATTAACTGCTTCTTTACTGTCATTATTTGCTCTTGTAGCTTTAATAAAAGAAGCTAATAAAAGAATTAACAAAATAGCTAATAATGAGTAAAAACAAATTACAATCATCTTACGACGATTTTTA  >  USA300TCH1516_ALE/691449‑691726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`