Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,945,299 G→T 6.1% intergenic (‑880/‑84) splA ← / → USA300HOU_RS09665 Serine protease SplA/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,945,2990GT 6.1% 506.0 / 3.2 163intergenic (‑880/‑84)splA/USA300HOU_RS09665Serine protease SplA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (65/81);  new base T (10/2);  total (79/84)
Fisher's exact test for biased strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

ATATTTATCTACATCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAGTATTAACAAC  >  USA300TCH1516_ALE/1945160‑1945438
                                                                                                                                           |                                                                                                                                           
atatTTATCTACATCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGa                                                                                                                                            >  1:778920/1‑141 (MQ=255)
  atTTATCTACATCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGata                                                                                                                                          <  1:1033231/141‑1 (MQ=255)
    ttATCTACATCGTAATATTCTTATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATGCATTTTTGAGAtt                                                                                                                                        <  4:298093/141‑1 (MQ=255)
         tACATCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGtt                                                                                                                                   <  2:637501/141‑1 (MQ=255)
          aCATCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTa                                                                                                                                  <  2:980098/141‑1 (MQ=255)
            aTCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAAt                                                                                                                                >  2:434932/1‑141 (MQ=255)
              ctcAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTg                                                                                                                              <  4:411817/141‑1 (MQ=255)
                 aaaaTTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAAtaaaaa                                                                                                                           <  4:105634/141‑6 (MQ=255)
                   aaTTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTTTTTTTTTTTTAATTGTTCtt                                                                                                                         >  4:294927/1‑141 (MQ=255)
                   aaTTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCtt                                                                                                                         >  3:290960/1‑141 (MQ=255)
                   aaTTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCtt                                                                                                                         <  2:534836/141‑1 (MQ=255)
                      tctcATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTa                                                                                                                      <  2:769418/141‑1 (MQ=255)
                      tctcATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTa                                                                                                                      <  2:551658/141‑1 (MQ=255)
                        tcatcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTTTTTTTTTTTTAATTGTTCTTTTaaa                                                                                                                    >  2:1064342/1‑141 (MQ=255)
                        tcatcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaa                                                                                                                    >  1:138065/1‑141 (MQ=255)
                        tcatcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaa                                                                                                                    >  2:848271/1‑141 (MQ=255)
                        tcatcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaa                                                                                                                    >  3:500728/1‑141 (MQ=255)
                         catcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaaa                                                                                                                   >  1:800003/1‑141 (MQ=255)
                          atcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGTTTTTTTGTTAATTGTTCTTTTaaaaa                                                                                                                  >  4:7642/1‑141 (MQ=255)
                          atcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaaaa                                                                                                                  >  1:227504/1‑141 (MQ=255)
                          atcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaaaa                                                                                                                  >  1:227507/1‑141 (MQ=255)
                          atcaACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaaaa                                                                                                                  >  2:977778/1‑141 (MQ=255)
                            caacaaTCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAat                                                                                                                >  2:437203/1‑141 (MQ=255)
                               caaTCTTTCACATCCAACATTTTTACTTTAGTTTTTATGATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAattat                                                                                                             <  2:183012/141‑1 (MQ=255)
                               caaTCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAattat                                                                                                             <  3:92750/141‑1 (MQ=255)
                                 aTCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAattatta                                                                                                           >  1:118123/1‑141 (MQ=255)
                                   cTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAattattatt                                                                                                         >  2:786316/1‑141 (MQ=255)
                                     ttCACATCCTACATTTTTACTTTAGTTTCTATAATTGTAAACAATCTTTCTATGTTAAATAATTATTCTGTTTTTTAGGTAATAGATAGTTAATTCATTTTTGTTATTTAGTTAAGTGTTCTTTTAAAAAATtattattat                                                                                                       <  1:859396/141‑1 (MQ=255)
                                      tCACATCTAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATtata                                                                                                      >  3:444203/1‑141 (MQ=255)
                                      tCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTTTTTTTTATATAGTTAATACATTTTTGATATTTATTTAATTGTTCTTTTAAAAAATTATTATtata                                                                                                      >  2:597300/1‑141 (MQ=255)
                                      tCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATtata                                                                                                      >  3:341779/1‑141 (MQ=255)
                                      tCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAATTAATAGATAGTTAATACATTTTTAATATTTATTTAATTATTTTTTTAAAAAATtattattatt                                                                                                      >  4:458101/1‑140 (MQ=255)
                                       cacaTCCAACTTTTTTACTTAAGTTTTTATAATTCAAATAAACAAAACTATGTTAAAAAATTATTCTATCTTTTAGTTAATAGATAGTTAATACATTTTTGTTTTTTAGTTAATAAAACATATAAAAAATTATTATTatat                                                                                                     <  2:1011046/141‑1 (MQ=255)
                                       cacaTCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTatat                                                                                                     >  1:1162430/1‑141 (MQ=255)
                                           tCCAACATTTTTACTTTAGATTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTTTTTTAAAAAATTATTATTATCTTTTc                                                                                                 >  1:840/1‑141 (MQ=255)
                                            ccAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCa                                                                                                >  2:734866/1‑141 (MQ=255)
                                            caaaCATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAAAAAAAAATAAAAAAAATTATTATTATATTTTCa                                                                                                <  4:378264/139‑1 (MQ=255)
                                             cAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCAt                                                                                               <  3:349948/141‑1 (MQ=255)
                                             cAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCAt                                                                                               <  3:102742/141‑1 (MQ=255)
                                               aCATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTg                                                                                             <  1:1047758/141‑1 (MQ=255)
                                                 aTTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTGTGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTa                                                                                           <  2:662550/141‑1 (MQ=255)
                                                 aTTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTGATTGAAAATTTAAAAAATTATTATTATATTTTCATTGTa                                                                                           <  2:290897/141‑1 (MQ=255)
                                                 aTTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTa                                                                                           >  1:66618/1‑141 (MQ=255)
                                                    tttACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAAc                                                                                        >  1:1092392/1‑141 (MQ=255)
                                                    tttACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAAc                                                                                        <  3:135297/141‑1 (MQ=255)
                                                     ttACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTCGTTAATTGTTCTTTTAAAACATTATTATTATCTTTTCATTGTAAACg                                                                                       >  3:22178/1‑141 (MQ=255)
                                                      tACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGt                                                                                      >  1:529489/1‑141 (MQ=255)
                                                       aCTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGtt                                                                                     >  1:1054927/1‑141 (MQ=255)
                                                       aCTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGtt                                                                                     >  2:481066/1‑141 (MQ=255)
                                                        cTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGttt                                                                                    <  2:122053/141‑1 (MQ=255)
                                                            aGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACaa                                                                                <  1:407379/141‑1 (MQ=255)
                                                             gTTTTTATAATTCAAAACAACTAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAat                                                                               <  3:85163/141‑1 (MQ=255)
                                                              tttttATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAata                                                                              <  1:585433/141‑1 (MQ=255)
                                                               ttttATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAatat                                                                             <  3:181530/141‑1 (MQ=255)
                                                                 ttATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATaa                                                                           <  4:4889/141‑1 (MQ=255)
                                                                       ttCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTAGATTTTCATTGTAAACGTTTACAATATAAAAAAAg                                                                     >  1:1103151/1‑141 (MQ=255)
                                                                         cAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGa                                                                   >  2:4771/1‑141 (MQ=255)
                                                                         aaaaaCAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAATTAAAAAAATATTAATATTTAGTTAAAAGTTCAATTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATaaaaaaaaaa                                                                   <  2:890772/140‑4 (MQ=255)
                                                                          aaaaCAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAg                                                                  >  1:442713/1‑141 (MQ=255)
                                                                           aaaCAACAAATATATATTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTTATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTAAATTTTCATTATAAAAGTTTAAAATATAAAAAAAGGAGc                                                                 <  2:943234/141‑1 (MQ=255)
                                                                           aaaCAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGc                                                                 <  1:520397/141‑1 (MQ=255)
                                                                           aaaCAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGc                                                                 <  4:315662/141‑1 (MQ=255)
                                                                           aaaCAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGc                                                                 <  1:1044201/141‑1 (MQ=255)
                                                                             acaacaAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTAAAATATAAAAAAAGGAGCaa                                                               <  2:292968/141‑1 (MQ=255)
                                                                              caacaaAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAAt                                                              >  3:230575/1‑141 (MQ=255)
                                                                                acaaAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATaa                                                            >  1:762897/1‑141 (MQ=255)
                                                                                 caaAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATaaa                                                           >  1:88217/1‑141 (MQ=255)
                                                                                   aaaCTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTaaaaa                                                                                                                  >  1:926588/1‑84 (MQ=255)
                                                                                   aaaCTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATaaaaa                                                         <  1:707234/141‑1 (MQ=255)
                                                                                     aCTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATg                                                       >  1:701874/1‑141 (MQ=255)
                                                                                       tATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTTTTTTTTTTTTAATTGTTCTTTTACATAATTATTATTATATTTTCATTGTAAAAGTTTACAGTGTAAATTCAGGCGCATTAAAAATGaa                                                     >  2:109666/1‑141 (MQ=255)
                                                                                       tATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATCAAGTGAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGaa                                                     <  1:323786/141‑1 (MQ=255)
                                                                                        aTGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAAt                                                    <  2:1083478/141‑1 (MQ=255)
                                                                                          gTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTATAAAAAATAAAAAAAGGAGCAATAAAAATGAATAc                                                  <  2:920878/141‑1 (MQ=255)
                                                                                           ttAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTTTTTTTTTTTTAATTGTTCTTTTAAAAAATAATTATTATATTTTTATTGTAAACGTTTACAATATAAATAAATGTGCAATAAATATGAATACa                                                 >  4:169020/1‑141 (MQ=255)
                                                                                           atAAAAAAATATTCAATATTTTAATTAATATTTATTTAATACAATTTTGATATTTAGTTAATTGTTCTTTTAAAATATAATTATTATATTTTCATTGTATAAGTAAAAAAAAAAAAAAAAGGAGCAATAAAAATGAATACa                                                 <  4:220138/140‑1 (MQ=255)
                                                                                            tAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACaa                                                <  3:386612/141‑1 (MQ=255)
                                                                                                aaaTTATTATATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTAAAAAAAAAAAAAAAAGGAGCAATAAAAATGAATACAAAAtt                                            <  2:6077/141‑1 (MQ=255)
                                                                                                   ttgttCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAAttttt                                         >  4:406392/4‑141 (MQ=255)
                                                                                                     attCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAg                                       <  2:458870/141‑1 (MQ=255)
                                                                                                       tCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGt                                     <  2:40530/141‑1 (MQ=255)
                                                                                                       tCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGt                                     <  1:108789/141‑1 (MQ=255)
                                                                                                       tCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTAAAAAAAATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGt                                     <  2:200435/141‑1 (MQ=255)
                                                                                                        cTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTa                                    >  1:551543/1‑141 (MQ=255)
                                                                                                               ttAGTTAATAGATAGTTAATACATTTTTTATATTTTGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAAcccg                             >  2:1178368/1‑138 (MQ=255)
                                                                                                               ttAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACAtt                             >  1:422454/1‑141 (MQ=255)
                                                                                                                  gTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATtagt                          >  1:1024990/1‑141 (MQ=255)
                                                                                                                      atagatagTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCa                      <  1:920157/141‑1 (MQ=255)
                                                                                                                       tagatagTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCaa                     >  1:932294/1‑141 (MQ=255)
                                                                                                                           tagTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGc                 >  2:281625/1‑141 (MQ=255)
                                                                                                                           tagTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGc                 <  2:778920/141‑1 (MQ=255)
                                                                                                                             gTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCtt               <  1:150350/141‑1 (MQ=255)
                                                                                                                                tctACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAg            <  3:356310/139‑1 (MQ=255)
                                                                                                                                aaTACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAg            <  3:406445/141‑1 (MQ=255)
                                                                                                                                aaTACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAg            >  1:994687/1‑141 (MQ=255)
                                                                                                                                 aTACATTTTTTTTTTTTTTTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTATGTAAAACATTAGTAGCAAGTGCTTTAGt           >  2:211846/1‑141 (MQ=255)
                                                                                                                                 aTACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAGt           >  2:144677/1‑141 (MQ=255)
                                                                                                                                         ttGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAGTATTaacaa   <  3:387630/141‑1 (MQ=255)
                                                                                                                                         ttGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAGTATTaacaa   <  1:174227/141‑1 (MQ=255)
                                                                                                                                          tGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTACTTTAGTATTaacaac  >  2:1173611/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATATTTATCTACATCTCAAAATTCTCATCAACAATCTTTCACATCCAACATTTTTACTTTAGTTTTTATAATTCAAAACAACAAAACTATGTTAAAAAATTATTCTATTTTTTAGTTAATAGATAGTTAATACATTTTTGATATTTAGTTAATTGTTCTTTTAAAAAATTATTATTATATTTTCATTGTAAACGTTTACAATATAAAAAAAGGAGCAATAAAAATGAATACAAAATTTTTAGGTAAAACATTAGTAGCAAGTGCTTTAGTATTAACAAC  >  USA300TCH1516_ALE/1945160‑1945438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: