Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,172,081 G→C 5.4% G44R (GGT→CGT)  leuB → 3‑isopropylmalate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,172,0810GC 5.4% 497.2 / 19.1 148G44R (GGT→CGT) leuB3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (53/86);  new base C (5/3);  total (59/89)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

Only 100 of 159 total aligned reads displayed.

GGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACAAGGATTA  >  USA300TCH1516_ALE/2171941‑2172221
                                                                                                                                             |                                                                                                                                             
ggtaATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTtg                                                                                                                                               >  2:515400/1‑141 (MQ=255)
 gtaATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTtgg                                                                                                                                              >  2:209910/1‑141 (MQ=255)
    attaattaTGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTtggtgg                                                                                                                                           <  1:131371/141‑1 (MQ=255)
    attaattaTGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTtggtgg                                                                                                                                           <  4:310311/141‑1 (MQ=255)
      taattaTGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTACTTATTAAATAGAGCACTACGAATTTggttgtt                                                                                                                                         >  4:43007/1‑140 (MQ=255)
       aattaTGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACCAAATTCGTGGTGc                                                                                                                                        <  1:360602/141‑1 (MQ=255)
        attattACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCCCCACGAATTTGGTGGTGcc                                                                                                                                       <  1:470839/141‑1 (MQ=255)
        attaTGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGcc                                                                                                                                       <  1:764452/141‑1 (MQ=255)
            tGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTa                                                                                                                                   >  1:809787/1‑141 (MQ=255)
             gACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACCAAATTCGTGGTGCTCTAtt                                                                                                                                <  1:238714/141‑1 (MQ=255)
                ttATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCCCGAATTTGGTGGTGCATCTATTGa                                                                                                                              >  1:516130/1‑141 (MQ=255)
                   taACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATAc                                                                                                                           >  2:530993/1‑141 (MQ=255)
                    aaCATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACAACGAATTTGGTGGTGCCTCTATTGATACa                                                                                                                          >  3:65535/1‑141 (MQ=255)
                     aCATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCACATAGAGCACCACGAATTTGGTGGTGCCTCTACTGATACAt                                                                                                                         <  2:710658/141‑1 (MQ=255)
                     aCATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACAt                                                                                                                         <  3:152123/141‑1 (MQ=255)
                          gttgCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCCCTATTGATACATTCGGc                                                                                                                    >  1:755829/1‑141 (MQ=255)
                           ttgCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCg                                                                                                                   <  1:41657/141‑1 (MQ=255)
                            tgCCCTACCTGGTGCTGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGCAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGCTGACTCTATTGATACATTCCGCGa                                                                                                                  >  2:109707/1‑141 (MQ=255)
                             gCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAg                                                                                                                 <  4:53320/141‑1 (MQ=255)
                               ccTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGcc                                                                                                               <  2:1092721/141‑1 (MQ=255)
                               ccTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGcc                                                                                                               >  1:412585/1‑141 (MQ=255)
                               ccTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGcc                                                                                                               <  1:530993/141‑1 (MQ=255)
                                cTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCt                                                                                                              <  1:891917/141‑1 (MQ=255)
                                cTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAACAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCt                                                                                                              >  1:466193/1‑141 (MQ=255)
                                 tACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCATCTATTGATACATTCGGCGCGCCtt                                                                                                             >  2:776157/1‑141 (MQ=255)
                                    cTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTaa                                                                                                          >  2:1151013/1‑141 (MQ=255)
                                    cTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTaa                                                                                                          >  1:802693/1‑141 (MQ=255)
                                      ggTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACt                                                                                                        <  2:23578/141‑1 (MQ=255)
                                      ggTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACt                                                                                                        <  1:935910/141‑1 (MQ=255)
                                      ggTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACt                                                                                                        <  2:434214/141‑1 (MQ=255)
                                      ggTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACt                                                                                                        <  1:653443/141‑1 (MQ=255)
                                       gTGATGGAATCGGTCCAGAAATTTTGAACCGTTTTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAATTAGAGCATCATGTATTTTGTGGTTCCTCTATTAATATATTCTTCGAGCCTTTAACtc                                                                                                       >  4:66881/1‑140 (MQ=255)
                                        tGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTga                                                                                                      <  1:1067075/141‑1 (MQ=255)
                                         gATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTgag                                                                                                     >  4:364734/1‑141 (MQ=255)
                                         gATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTgag                                                                                                     >  1:996115/1‑141 (MQ=255)
                                         gATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTgag                                                                                                     >  2:885465/1‑141 (MQ=255)
                                         gATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTCTAACTgag                                                                                                     >  2:311665/1‑141 (MQ=255)
                                          aTGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTgaga                                                                                                    >  4:360598/1‑141 (MQ=255)
                                              aaTCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAAc                                                                                                <  1:701685/141‑1 (MQ=255)
                                              aaTCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAAc                                                                                                <  2:535089/141‑1 (MQ=255)
                                                tCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCt                                                                                              <  2:483361/141‑1 (MQ=255)
                                                tCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCt                                                                                              <  4:282362/141‑1 (MQ=255)
                                                 cGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACt                                                                                                        <  3:247526/130‑1 (MQ=255)
                                                 cGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACt                                                                                                        >  4:247526/1‑130 (MQ=255)
                                                 cGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCtt                                                                                             <  2:578420/141‑1 (MQ=255)
                                                      cAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATg                                                                                        <  1:846273/141‑1 (MQ=255)
                                                       aGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGc                                                                                       <  2:257655/141‑1 (MQ=255)
                                                        gAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAGGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCg                                                                                      <  4:440179/141‑1 (MQ=255)
                                                        gAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCg                                                                                      >  2:182561/1‑141 (MQ=255)
                                                        aaaaTTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCg                                                                                      <  3:104319/140‑1 (MQ=255)
                                                          aaTTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCgtg                                                                                    <  1:1157812/141‑1 (MQ=255)
                                                               tGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTaaaa                                                                               <  1:1177276/141‑1 (MQ=255)
                                                               tGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTaaaa                                                                               >  2:471085/1‑141 (MQ=255)
                                                               tGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTaaaa                                                                               <  3:84796/141‑1 (MQ=255)
                                                                gAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATAc                                                                                                                           <  1:70275/96‑1 (MQ=255)
                                                                gAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATAc                                                                                                                           >  2:70275/1‑96 (MQ=255)
                                                                gAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAag                                                                              >  1:1105081/1‑141 (MQ=255)
                                                                         tctATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGc                                                                     <  1:293633/141‑1 (MQ=255)
                                                                          ctATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAATAGCatctgct                                                                    <  3:334837/141‑7 (MQ=255)
                                                                            aTCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTAt                                                                  <  2:369509/141‑1 (MQ=255)
                                                                             tCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTAtt                                                                 <  2:338729/141‑1 (MQ=255)
                                                                             tCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTAtt                                                                 <  3:220900/141‑1 (MQ=255)
                                                                               aTTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTAtttt                                                               <  3:243999/141‑1 (MQ=255)
                                                                                  gCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACt                                                            <  4:26551/141‑1 (MQ=255)
                                                                                    ttGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTgg                                                          <  1:485100/141‑1 (MQ=255)
                                                                                    ttGAAATGATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTgg                                                          >  3:399278/1‑141 (MQ=255)
                                                                                     tGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTggg                                                         <  3:52972/141‑1 (MQ=255)
                                                                                       aaaTTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTg                                                       <  1:225207/141‑1 (MQ=255)
                                                                                        aaTTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGc                                                      <  4:390351/141‑1 (MQ=255)
                                                                                         aTTATAAGTAATAAATATATCTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCa                                                     <  3:163592/141‑1 (MQ=255)
                                                                                         aTTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCa                                                     >  1:1065679/1‑141 (MQ=255)
                                                                                            ataAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATc                                                  <  1:182561/141‑1 (MQ=255)
                                                                                             taAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCg                                                 >  1:942968/1‑141 (MQ=255)
                                                                                                   ataaataTAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAAAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGAc                                           <  1:601056/141‑1 (MQ=255)
                                                                                                    taaataTAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGAcc                                          <  1:139774/141‑1 (MQ=255)
                                                                                                     aaataTAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCt                                         >  1:799458/1‑141 (MQ=255)
                                                                                                         ataACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAAt                                     <  2:802693/141‑1 (MQ=255)
                                                                                                         ataACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAAt                                     >  1:544029/1‑141 (MQ=255)
                                                                                                         ataACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAAt                                     >  4:346234/1‑141 (MQ=255)
                                                                                                           aaCTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATgg                                   <  4:134373/141‑1 (MQ=255)
                                                                                                            aCTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGa                                  <  2:863812/141‑1 (MQ=255)
                                                                                                             cTTTAATTATCAAATAGAGCACCACGAATTTGGTGATGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGAc                                 >  4:479803/1‑141 (MQ=255)
                                                                                                                 aaTTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGAt                             >  2:570713/1‑141 (MQ=255)
                                                                                                                  aTTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATc                            <  1:923133/141‑1 (MQ=255)
                                                                                                                    tATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCt                          >  1:336078/1‑141 (MQ=255)
                                                                                                                       cAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGTTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAAc                       <  3:416695/141‑1 (MQ=255)
                                                                                                                         aaTAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACaa                     <  1:530616/141‑1 (MQ=255)
                                                                                                                          aTAGAGGCACCACCAAATTCGTGGTGCTCTATTTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGAGCCTAACaa                     >  1:1028001/1‑141 (MQ=37)
                                                                                                                            agagCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCg                  >  1:367063/1‑141 (MQ=255)
                                                                                                                             gagCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCgc                 >  4:209371/1‑140 (MQ=255)
                                                                                                                              agCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGAc                >  1:1022166/1‑141 (MQ=255)
                                                                                                                                gCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGAcc               >  2:982337/1‑141 (MQ=255)
                                                                                                                                gCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGAcc               >  1:458759/1‑141 (MQ=255)
                                                                                                                                   ccacGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGa            <  1:282352/141‑1 (MQ=255)
                                                                                                                                      cGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACa         <  2:630551/141‑1 (MQ=255)
                                                                                                                                         aTTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACAAgg      <  3:381098/141‑1 (MQ=255)
                                                                                                                                          tttGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACAAGGa     <  2:1006999/141‑1 (MQ=255)
                                                                                                                                          tttGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACAAGGa     >  2:231222/1‑141 (MQ=255)
                                                                                                                                           ttGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTa                                        >  2:914282/1‑105 (MQ=255)
                                                                                                                                             ggtggtGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACAAGGatta  >  3:381519/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACAGATCCTAACAATCGACCAGAACAAGGATTA  >  USA300TCH1516_ALE/2171941‑2172221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: