BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	51,740	C→A	8.6%	Q134K (CAA→AAA)	USA300HOU_RS00215 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	51,740	0	C	A	8.6%	597.7 / 7.9	192	Q134K (CAA→AAA)	USA300HOU_RS00215	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (105/65); new base A (2/19); total (107/85)
Fisher's exact test for biased strand distribution p-value = 5.98e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

GTCAAAATAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTTAT  >  USA300TCH1516_ALE/51600‑51880

gTCAAAATAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGc                                                                                                                                              >  1:987592/1‑141 (MQ=255)

gTCAAAATAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGc                                                                                                                                              >  1:1054829/1‑141 (MQ=255)

gTCAAAATAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGc                                                                                                                                              <  1:557021/141‑1 (MQ=255)

  cAAAATAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCaa                                                                                                                                            <  1:683032/141‑1 (MQ=255)

   aaaaTAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCaaa                                                                                                                                           <  2:960762/141‑1 (MQ=255)

   aaaaTAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCaaa                                                                                                                                           <  1:317257/141‑1 (MQ=255)

     aataataatTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCaaaaa                                                                                                                                         >  1:117579/1‑141 (MQ=255)

          taatTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGAtt                                                                                                                                    >  2:827683/1‑141 (MQ=255)

             ttttAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGt                                                                                                                                 <  3:315001/141‑1 (MQ=255)

             ttttAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGt                                                                                                                                 <  4:436728/141‑1 (MQ=255)

             ttttAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGt                                                                                                                                 >  4:320704/1‑141 (MQ=255)

              tttAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGtt                                                                                                                                <  2:674510/141‑1 (MQ=255)

              tttAAACGTAAACAGTATAAAAATAAAGAAAAATTGAATTAACTACGAGCGAAAAAGAAAGTAGATCCATATAGGATTTATGATTCAAATATTATTTCAAAAGCATTGGCAGAAATAAAA‑AAAAAAAAAAAAAGATTAGtt                                                                                                                                <  4:413597/141‑1 (MQ=255)

              tttAAACATAAAACATTTAAAAATAAAAAAAAATTGAAATCACTACTAGAGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATAATACTTCAAAAGCATTGGCAGAAATAAAA‑AAAAAAAAAAAAAGATTAGtt                                                                                                                                <  2:487562/141‑1 (MQ=255)

                 aaaCGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACa                                                                                                                             <  1:1086843/141‑1 (MQ=255)

                 aaaCGTAATCAAATTATAAATGAAGAAAAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAA‑AAAAAAAAAAAAAGATTAGTTACa                                                                                                                             <  2:636961/141‑1 (MQ=255)

                   aCGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACaaa                                                                                                                           >  2:110023/1‑141 (MQ=255)

                      tAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGAt                                                                                                                        <  1:61231/141‑1 (MQ=255)

                          ccATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGac                                                                                                                    >  2:529128/1‑141 (MQ=255)

                               tataAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACAct                                                                                                               <  1:1073866/141‑1 (MQ=255)

                                 taAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACActct                                                                                                             >  2:361503/1‑141 (MQ=255)

                                   aaTGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCATATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCtat                                                                                                           >  2:604618/1‑141 (MQ=255)

                                    aTGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCtata                                                                                                          >  1:1045824/1‑141 (MQ=255)

                                     tGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATaa                                                                                                         >  2:845954/1‑141 (MQ=255)

                                       aagaaCAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATc                                                                                                       >  2:729465/1‑141 (MQ=255)

                                       aagaaCAATTGAAATCACTAATCGCGATAAAGAAAGTAGATGCACATATGAGATATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACGCTATAATc                                                                                                       >  2:707276/1‑141 (MQ=255)

                                        agaaCAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCa                                                                                                      <  3:381330/141‑1 (MQ=255)

                                        agaaCAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCa                                                                                                      >  4:478080/1‑141 (MQ=255)

                                        agaaCAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCa                                                                                                      >  2:350076/1‑141 (MQ=255)

                                          aaCAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGt                                                                                                    >  2:377938/1‑141 (MQ=255)

                                            cAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCa                                                                                                  >  3:160812/1‑141 (MQ=255)

                                             aaTTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCaa                                                                                                 >  1:664026/1‑141 (MQ=255)

                                              aTTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCaaa                                                                                                >  3:344053/1‑141 (MQ=255)

                                              aTTAAAATCACAACTCGAGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAACAAAAACAAAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCaa                                                                                                 <  2:120802/141‑1 (MQ=255)

                                                tGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCaaaaa                                                                                              <  1:601876/141‑1 (MQ=255)

                                                  aaaTCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCaaaaaaa                                                                                            >  2:1043997/1‑141 (MQ=255)

                                                   aaTCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAg                                                                                           >  2:341355/1‑141 (MQ=255)

                                                    aTCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGa                                                                                          >  1:767613/1‑141 (MQ=255)

                                                     tCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGaa                                                                                         >  1:594892/1‑141 (MQ=255)

                                                       actactCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGaaaa                                                                                       <  1:1119517/141‑1 (MQ=255)

                                                         tactCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATg                                                                                     >  2:989256/1‑141 (MQ=255)

                                                             cgcgATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAata                                                                                 >  2:798119/1‑141 (MQ=255)

                                                              gcgATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAatat                                                                                >  1:88538/1‑141 (MQ=255)

                                                                  tAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTAc                                                                            >  4:405419/1‑141 (MQ=255)

                                                                    aagaaagTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACgg                                                                          >  1:286653/1‑141 (MQ=255)

                                                                     agaaagTAGATGCACATATGAGTTATGATTCAAATATTACTTCGACAGCGTTGGCAAAACTCAAC‑AAAAGGTAAAAAAGATTAGGTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGt                                                                         <  3:97851/141‑1 (MQ=255)

                                                                     agaaagTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACGCACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGt                                                                         >  2:4379/1‑141 (MQ=255)

                                                                       aaagTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTa                                                                       >  4:199404/1‑141 (MQ=255)

                                                                         agTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAgg                                                                     >  1:1000792/1‑141 (MQ=255)

                                                                          gTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGga                                                                    <  1:1033886/141‑1 (MQ=255)

                                                                          gTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGga                                                                    >  1:94258/1‑141 (MQ=255)

                                                                            aGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGgaga                                                                  <  4:394123/141‑1 (MQ=255)

                                                                            aGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGgaga                                                                  >  1:729608/1‑141 (MQ=255)

                                                                               tGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGaaaa                                                               >  1:1007665/1‑141 (MQ=255)

                                                                               tGCACATATGAGTTATGATACAAATATTACTTACACAGCATTGGCCGAAAAAAAC‑CAAACACAAACCCGCCTCGTTACCAACATACACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGaaaa                                                               <  1:712850/141‑1 (MQ=255)

                                                                                 cacaTATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAGATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGaaaaaa                                                             >  3:418416/1‑141 (MQ=255)

                                                                                     tatGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATc                                                         >  1:294187/1‑141 (MQ=255)

                                                                                           ttATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATAc                                                   <  2:111213/141‑1 (MQ=255)

                                                                                           ttATGATTCAAATATTACTTCAAAAACATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATAc                                                   >  2:30986/1‑141 (MQ=255)

                                                                                           ttATGATTAAAATAATAAAAAAAAAAAATTGGCAGAAAAAAAA‑AAAACGAAAAAAAGATTAGTTACAAAGATAAACACACACTATAATAAATCAAAAAAAGAAAATGAATATGTAAAAAAAAAAAAAAAAATATCGTATAc                                                   <  2:566842/141‑1 (MQ=255)

                                                                                           ttATGATAAAAATAATACTACAAAAGAATTGACAGAAAAAAAA‑AAAAAGAAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATAc                                                   <  2:1045824/141‑1 (MQ=255)

                                                                                                   cAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTgg                                           <  3:444570/141‑1 (MQ=255)

                                                                                                   cAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTgg                                           >  4:499380/1‑141 (MQ=255)

                                                                                                   cAAATATTACTTCAAAAGCATTGGCAGAAATAAAA‑AAAACAAAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTgg                                           <  4:220754/141‑1 (MQ=255)

                                                                                                     aaTATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGgag                                         >  4:347649/1‑141 (MQ=255)

                                                                                                         ttACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGCATc                                     >  3:130/1‑141 (MQ=255)

                                                                                                           aCTTCAAAAGCATTGGCAGAAATAAAC‑ACAACGAAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATCAAAAATTGGCGAATCAt                                   >  2:1005039/1‑141 (MQ=255)

                                                                                                           aCTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCAt                                   >  2:886832/1‑141 (MQ=255)

                                                                                                            cTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCAtt                                  <  1:431537/141‑1 (MQ=255)

                                                                                                            cTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCAtt                                  <  3:485948/141‑1 (MQ=255)

                                                                                                             ttCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTg                                 >  2:56508/1‑141 (MQ=255)

                                                                                                             ttCAAAAGCATTAGCAAAAAAAAAC‑AAAAAAAAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTg                                 <  4:412863/141‑1 (MQ=255)

                                                                                                                  aaGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAat                            >  1:171289/1‑141 (MQ=255)

                                                                                                                  aaGCATTGGCAGAAATAAAC‑AAAACAAAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGACAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAat                            <  2:295837/141‑1 (MQ=255)

                                                                                                                      aTTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATAttt                        >  2:1118633/1‑141 (MQ=255)

                                                                                                                      aTTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATAttt                        >  3:232751/1‑141 (MQ=255)

                                                                                                                      aTTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATAttt                        >  2:278119/1‑141 (MQ=255)

                                                                                                                       ttGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTta                       >  2:1177896/1‑141 (MQ=255)

                                                                                                                             gAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAAt                 >  1:488386/1‑141 (MQ=255)

                                                                                                                              aaaTAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATGTATATAATg                >  4:82197/1‑141 (MQ=255)

                                                                                                                                aTAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATgag              <  1:379074/141‑1 (MQ=255)

                                                                                                                                  aaaC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGAt            >  3:178517/1‑141 (MQ=255)

                                                                                                                                   aaC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGAtt           >  3:298961/1‑141 (MQ=255)

                                                                                                                                   aaC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGAtt           >  3:289432/1‑141 (MQ=255)

                                                                                                                                   aaC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGAtt           >  3:493602/1‑141 (MQ=255)

                                                                                                                                   aaC‑AAAAAGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGAtt           >  4:453063/1‑141 (MQ=255)

                                                                                                                                    aC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAACGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTc          >  2:890810/1‑141 (MQ=255)

                                                                                                                                     c‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCa         <  2:59959/141‑1 (MQ=255)

                                                                                                                                        aaaCGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGt       <  2:206499/141‑1 (MQ=255)

                                                                                                                                        aaaCGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGt       <  2:665835/141‑1 (MQ=255)

                                                                                                                                           cGCAAAAAATATGAGTAACAAAGATAGACTCACCCTATAAAAAAAAAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGtttt    <  4:265785/141‑1 (MQ=255)

                                                                                                                                           cGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGtttt    <  2:1078456/141‑1 (MQ=255)

                                                                                                                                           cGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGtttt    <  3:266434/141‑1 (MQ=255)

                                                                                                                                           cGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTAAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGtttt    >  1:268543/1‑141 (MQ=255)

                                                                                                                                            gCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTta   >  1:586911/1‑141 (MQ=255)

                                                                                                                                            gCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTta   >  1:227933/1‑141 (MQ=255)

                                                                                                                                             cAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTtat  <  1:30986/141‑1 (MQ=255)

                                                                                                                                             cAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTtat  >  1:201173/1‑141 (MQ=255)

                                                                                                                                             cAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTtat  >  1:196609/1‑141 (MQ=255)

                                                                                                                                             cAAAAAAGATTAGTTACAAAGATAGACAAACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTtat  <  1:463735/141‑1 (MQ=255)

GTCAAAATAATAATTTTAAACGTAATCCATTTATAAATGAAGAACAATTGAAATCACTACTCGCGATAAAGAAAGTAGATGCACATATGAGTTATGATTCAAATATTACTTCAAAAGCATTGGCAGAAATAAAC‑AAAACGCAAAAAAGATTAGTTACAAAGATAGACACACTCTATAATCAGTCAAAAAAAGAAAATGAATATGTACGGTTAGGAGAAAAAATATCGTATACAAAATTGGAGAATCATTGGAAATATTTATATAATGAGATTCAGTTTTAT  >  USA300TCH1516_ALE/51600‑51880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`