BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,291,888	G→A	5.6%	K813K (AAG→AAA)	USA300HOU_RS11675 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,291,888	0	G	A	5.6%	455.2 / 11.4	155	K813K (AAG→AAA)	USA300HOU_RS11675	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (84/61); new base A (3/6); total (88/67)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 158 total aligned reads displayed.

CAAAAGACGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTA  >  USA300TCH1516_ALE/2291764‑2292028

cAAAAGACGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTgg                                                                                                                              >  1:546172/1‑141 (MQ=32)

 aaaaGACGATATACTACGACGCTCCTACGAACTTGTCCAAGGATTAAGAAAAGATCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAAGGTGTATGGAAAGTGGt                                                                                                                             <  2:814253/141‑1 (MQ=11)

 aaaaGACGATATACTACGACGCTCCTACGAACTTGTCCAAGGATTAAGAAAAGATCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAAGGTGTATGGAAAGTGGt                                                                                                                             <  2:668147/141‑1 (MQ=11)

  aaaGACGATATACTACGACGCTCCTACGAACTTGTCCAAGGATTAAGAAAAGATCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAAGGTGTATGGAAAGTGGtt                                                                                                                            >  1:225647/1‑141 (MQ=11)

  aaaGACGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGGAAAAAGTCTGTGAGTGAGGGTGTATGGAAAGTGGtt                                                                                                                            >  4:504713/1‑141 (MQ=17)

   aaGACGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAATGGTGTATGGAAAGTGGTTa                                                                                                                           >  2:1156695/1‑141 (MQ=17)

    aGACGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTaa                                                                                                                          >  3:312038/1‑141 (MQ=32)

      aCGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAat                                                                                                                        >  4:214106/1‑141 (MQ=32)

       cGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAata                                                                                                                       >  1:1012555/1‑141 (MQ=32)

       cGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAata                                                                                                                       >  3:320780/1‑141 (MQ=32)

        gATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAatat                                                                                                                      <  4:54304/141‑1 (MQ=32)

           ataCTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAAtattat                                                                                                                   <  2:884314/141‑1 (MQ=32)

           ataCTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAAtattat                                                                                                                   <  2:552820/141‑1 (MQ=32)

           ataCTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAAtattat                                                                                                                   <  4:312038/141‑1 (MQ=32)

              ctacGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGa                                                                                                                >  1:380515/1‑141 (MQ=32)

              ctacGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGa                                                                                                                >  3:187923/1‑141 (MQ=32)

               tacGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGaa                                                                                                               <  4:476112/141‑1 (MQ=32)

                 cGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGaaaa                                                                                                             >  2:440743/1‑141 (MQ=32)

                    cacTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACAt                                                                                                          <  1:197516/141‑1 (MQ=32)

                    cacTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACAt                                                                                                          <  1:864962/141‑1 (MQ=32)

                      cTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGaagatagaa                                                                                                        >  2:643868/1‑134 (MQ=17)

                      cTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCa                                                                                                        >  2:927003/1‑141 (MQ=32)

                      cTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCa                                                                                                        >  1:13056/1‑141 (MQ=32)

                      cTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCa                                                                                                        <  2:415109/141‑1 (MQ=32)

                        ccTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCaaa                                                                                                      >  2:902673/1‑141 (MQ=32)

                         cTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTT‑AATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAgg                                                                                                    <  3:362509/141‑1 (MQ=17)

                          tACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAgg                                                                                                    <  2:98664/141‑1 (MQ=32)

                           aCGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGa                                                                                                   >  1:298988/1‑141 (MQ=32)

                            cGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGAt                                                                                                  <  1:254955/141‑1 (MQ=32)

                                 ttGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTaa                                                                                             >  2:214759/1‑141 (MQ=32)

                                   gTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGa                                                                                           >  3:253437/1‑141 (MQ=32)

                                    tCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGaa                                                                                          >  3:12641/1‑141 (MQ=32)

                                     ccAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTAGTTAAATATTATAGAAAACATCAAAGGATGTTAAGaaa                                                                                         <  1:631324/141‑1 (MQ=17)

                                        aGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATAc                                                                                      >  4:168445/1‑141 (MQ=32)

                                            ttACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAAtt                                                                                  >  4:128639/1‑141 (MQ=32)

                                             tACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAAttt                                                                                 >  1:934564/1‑141 (MQ=32)

                                               cGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGt                                                                                                                             <  1:849827/95‑1 (MQ=34)

                                               cGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATttat                                                                               >  1:347874/1‑141 (MQ=32)

                                                gAAAAGCCCTAAGGTTATGTCATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGCAATAAAATttatt                                                                              >  4:170922/1‑141 (MQ=255)

                                                gAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGtt                                                                                               >  2:596229/1‑124 (MQ=32)

                                                gAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGtt                                                                                               <  1:596229/124‑1 (MQ=32)

                                                gAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATttatt                                                                              >  1:691813/1‑141 (MQ=32)

                                                gAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATttatt                                                                              >  1:1063227/1‑141 (MQ=32)

                                                gAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATttatt                                                                              >  2:697628/1‑141 (MQ=32)

                                                gAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATttatt                                                                              >  1:400826/1‑141 (MQ=32)

                                                    aGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTAccc                                                                          <  2:227199/141‑1 (MQ=32)

                                                    aGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGAATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGATAAGATATACAAGTTAGTAccc                                                                          >  2:1129837/1‑141 (MQ=11)

                                                      aCCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAg                                                                        >  3:275119/1‑141 (MQ=32)

                                                        cTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAAGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAACTACAATTTATTACCCAGCa                                                                      >  1:1144873/1‑141 (MQ=17)

                                                          aaGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCAtt                                                                    >  3:1641/1‑141 (MQ=32)

                                                           aGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCAttt                                                                   >  1:327865/1‑141 (MQ=32)

                                                                 tGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTaataat                                                             <  1:992958/141‑1 (MQ=32)

                                                                    aaTTGACCTAAATTTATTAATCGGTTGAATTAAGTTAGTAAAACGACTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACTTCAAAGGATGTTAAGACATACGATTTACTACCCAGCATTTAATCATgtt                                                          <  2:483972/141‑3 (MQ=11)

                                                                          ccTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCtata                                                    <  1:966891/141‑1 (MQ=32)

                                                                          ccTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCtata                                                    <  1:466806/141‑1 (MQ=32)

                                                                           cTAAATCTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGGGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAg                                                   <  2:1139191/141‑1 (MQ=17)

                                                                             aaaTTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGaa                                                 >  1:760954/1‑141 (MQ=32)

                                                                              aaTTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAg                                                <  2:735271/141‑1 (MQ=32)

                                                                                   attaATCGTTTAAATTCAGTTAGTAAAAAGGCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAAtt                                           >  2:301740/1‑141 (MQ=17)

                                                                                    ttaATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGCTGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATta                                          <  1:513063/141‑1 (MQ=17)

                                                                                    ttaATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATta                                          >  2:1042515/1‑141 (MQ=32)

                                                                                    ttaATCAGTTAAATTCAGTGAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATAAAATTTATTATTCAGCATTTAATAATGGTGTTATAGAAGGAATta                                          >  4:355377/1‑141 (MQ=11)

                                                                                     taATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaa                                         >  1:825124/1‑141 (MQ=32)

                                                                                      aaTCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaat                                        >  3:477511/1‑141 (MQ=32)

                                                                                         cGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataat                                     <  2:331065/141‑1 (MQ=32)

                                                                                         cGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataat                                     <  1:966721/141‑1 (MQ=32)

                                                                                         cGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataat                                     <  2:285322/141‑1 (MQ=32)

                                                                                         cGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataat                                     <  1:1144015/141‑1 (MQ=32)

                                                                                         cGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataat                                     >  2:869884/1‑141 (MQ=32)

                                                                                         cGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAAGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataat                                     <  2:732598/141‑1 (MQ=17)

                                                                                          gTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataata                                    >  1:832787/1‑141 (MQ=32)

                                                                                           tttAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATtaataataa                                   <  3:128639/141‑1 (MQ=32)

                                                                                            ttAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAg                                  >  2:476687/1‑141 (MQ=32)

                                                                                              aaaTTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGAt                                >  2:176013/1‑141 (MQ=32)

                                                                                              aaaTTAAGTTAGTAAAAATTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGAt                                <  4:271988/141‑1 (MQ=17)

                                                                                               aaTTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATa                               <  2:1082483/141‑1 (MQ=32)

                                                                                               aaTTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATa                               >  4:316836/1‑141 (MQ=32)

                                                                                               aaTGCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATa                               <  2:450289/141‑1 (MQ=17)

                                                                                                   cAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAAt                           <  1:220646/141‑1 (MQ=32)

                                                                                                   cAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAAt                           >  2:767598/1‑141 (MQ=32)

                                                                                                   cAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAAt                           >  1:967789/1‑141 (MQ=32)

                                                                                                   cAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAAt                           <  3:276907/141‑1 (MQ=32)

                                                                                                    aGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAAtt                          >  4:332480/1‑141 (MQ=32)

                                                                                                       tAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAAt                       >  2:78566/1‑141 (MQ=32)

                                                                                                       aaGTAAAAAGTCAGTGAGGAAGGGTGAATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTAAAGAAAAACAATTTATTACCCAGCAAACAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAAt                       >  2:306580/2‑141 (MQ=11)

                                                                                                        aGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATc                      >  2:895119/1‑141 (MQ=32)

                                                                                                              aaGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGa                <  2:406644/141‑1 (MQ=35)

                                                                                                              aaGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGa                <  4:57916/141‑1 (MQ=34)

                                                                                                               aGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGt                                                          <  2:1083560/98‑1 (MQ=34)

                                                                                                                gTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAAt              <  3:370779/141‑1 (MQ=35)

                                                                                                                 tCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAAtt             <  3:77045/141‑1 (MQ=35)

                                                                                                                 tCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAAtt             <  1:478870/141‑1 (MQ=35)

                                                                                                                  cTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAAttt            >  2:995954/1‑141 (MQ=35)

                                                                                                                   tgtgAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTACGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAAGAATAAGATAAAATTACTCAAGTGAATTTc           >  1:1019199/1‑141 (MQ=255)

                                                                                                                    gtgAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCt          >  3:87759/1‑141 (MQ=35)

                                                                                                                    gtgAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCt          <  1:736505/141‑1 (MQ=35)

                                                                                                                    gtgAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCt          >  1:1067940/1‑141 (MQ=35)

                                                                                                                    gtgAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCt          <  4:85482/141‑1 (MQ=35)

                                                                                                                     tgAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAATATTAATCAAGTGAATTTCtt         >  4:504686/1‑141 (MQ=25)

                                                                                                                            gggTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTa  <  2:178437/141‑1 (MQ=35)

CAAAAGACGATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTA  >  USA300TCH1516_ALE/2291764‑2292028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`