BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,981	A→G	6.7%	D735D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,981	0	A	G	6.7%	368.1 / 2.1	146	D735D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (61/72); new base G (3/9); total (65/81)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

AATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTC  >  USA300TCH1516_ALE/2774842‑2775121

actcACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                             <  2:200150/139‑1 (MQ=255)

aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTTCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                             >  1:535785/1‑141 (MQ=255)

           gagtctgAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTa                                                                                                                                  <  2:395992/135‑1 (MQ=255)

              tCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAAGCTGAATCGCTGTCTGGGAGCGAATCGCTATCGGAATCTGAGTCGCTGACTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtct                                                                                                                               >  4:62364/1‑141 (MQ=14)

               ccGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTAtctg                                                                                                                              >  1:414093/1‑141 (MQ=255)

                 gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgag                                                                                                                            >  1:885507/1‑141 (MQ=255)

                  aaTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctggct                                                                                                                           >  2:433148/1‑138 (MQ=255)

                   aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                                                                          <  1:510448/141‑1 (MQ=255)

                    tCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgaccct                                                                                                                         >  1:743924/1‑137 (MQ=255)

                       cTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgag                                                                                                                      >  1:1044292/1‑141 (MQ=255)

                         ttctgtatttgtaTCGCTATCCGGGTCTGCGTGGCTGTCTGACTCTGAGTCGCTGTCTGAGTCCGAGTCGCTATCTGGATCTGAGTCGCTGTCTGAGTCTGAGTCgctatctgagtctgagtcgctatctgagtctgagtc                                                                                                                    <  2:798234/140‑1 (MQ=14)

                         atctgaatctgaATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                                    <  4:148816/141‑1 (MQ=255)

                           ctggatctgaatCGCGTCCCGCGTCTGATTCGCTGTCTGAGCCTGGATCGCTGTCTGAGTCCGAATCGCTCTCTGCATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGAATCAGAGTCGCTAtctgagtctgagtcgc                                                                                                                  <  2:616210/141‑1 (MQ=11)

                           ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGACTCCCTGTCTGAGTCTGACTCGCTATCTGACTCTGATTCCCTGtccgagtccgactcgc                                                                                                                  >  2:864017/1‑141 (MQ=14)

                             gaatctgaatcGCTATCCGAGTCTGAGTCGCTCTATGAATCTGCATCGCTGTCTGAGTCCGATTAGCTCTATGAATCTGAGTCGCTGTCTGCCTCTGACTCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctg                                                                                                                <  1:894191/141‑1 (MQ=14)

                               gtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTTAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgaatcgctgtc                                                                                                              <  4:444976/140‑1 (MQ=11)

                               atctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc                                                                                                              <  4:180626/141‑1 (MQ=255)

                                    aatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTAtctgagtctgagtcgctgtctgagg                                                                                                         >  2:630098/1‑140 (MQ=255)

                                     atcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                                        >  1:274581/1‑141 (MQ=255)

                                      tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                                      >  1:1124700/1‑110 (MQ=255)

                                      tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                                      <  2:1124700/110‑1 (MQ=255)

                                      tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtct                                                                                                       >  1:103429/1‑141 (MQ=255)

                                      tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtct                                                                                                       <  2:1031956/141‑1 (MQ=255)

                                       cGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctg                                                                                                      <  3:79873/141‑1 (MQ=255)

                                         cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                                                                                    <  2:293048/141‑1 (MQ=255)

                                         cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                                                                                    >  2:177566/1‑141 (MQ=255)

                                         cTATCCGAGTCTGACTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                                                                                    <  4:350954/141‑1 (MQ=255)

                                           aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                                                                                  <  1:952404/141‑1 (MQ=255)

                                            tCCGAGTCTGAGTCGCTGTCTGAATCTAAATCTCAGACTGGGTCCGTATCGCTATCTGAATCTGAGTCGCTGTCTGTGTCTGAATCTCTATCTGAATCTGAGTCGCTAtctgaatctgaatccatgtctgaatccgagtca                                                                                                 >  2:53559/1‑140 (MQ=11)

                                             ccGTGTCTGAGTCGGTTTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgc                                                                                                <  2:126454/141‑1 (MQ=14)

                                             ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgc                                                                                                <  2:885507/141‑1 (MQ=255)

                                             ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgc                                                                                                <  1:555071/141‑1 (MQ=255)

                                                     gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                        >  1:629770/1‑141 (MQ=255)

                                                     gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                        >  2:1079721/1‑141 (MQ=255)

                                                       gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                                      <  1:405347/141‑1 (MQ=255)

                                                          gCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAgtcgctgtctgagtctgagtcgctgtctgagtctga                                                                                   >  3:48214/1‑141 (MQ=255)

                                                             gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                >  1:727120/1‑141 (MQ=255)

                                                             gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                <  1:266069/141‑1 (MQ=255)

                                                              tctgaatctgaaTCGCTGTCTGAGTCCGACTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCtgaatctgaatca                                                                               <  4:330366/141‑2 (MQ=11)

                                                              tctgaatctgaaTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTATGAATCg                                                                               >  3:107596/1‑141 (MQ=255)

                                                                   atctgagtcGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                          <  2:455564/141‑1 (MQ=11)

                                                                   atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                          <  4:281575/141‑1 (MQ=255)

                                                                   atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                          >  1:969788/1‑141 (MQ=255)

                                                                   atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                          <  1:131517/141‑1 (MQ=255)

                                                                    tctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatct                                                                         >  3:159043/1‑141 (MQ=255)

                                                                      tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                       <  2:1130108/141‑1 (MQ=255)

                                                                      tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                       >  1:786065/1‑141 (MQ=255)

                                                                      tgaatcGCTGTCTGAGGCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCCGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                       <  1:662027/141‑1 (MQ=255)

                                                                       gaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                      >  3:21033/1‑141 (MQ=255)

                                                                       gaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                      >  3:417728/1‑141 (MQ=255)

                                                                        aatcGCTGTCTGGGTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                     <  2:765618/141‑1 (MQ=14)

                                                                           cGCTGTCTGGGTCAGAATCTCTATCTGAAGCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                                                                  <  2:48794/141‑1 (MQ=17)

                                                                           cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTatctgaatctg                                                                  >  2:651864/1‑141 (MQ=255)

                                                                             cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                >  1:211082/1‑141 (MQ=255)

                                                                               gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                              <  4:131443/141‑1 (MQ=255)

                                                                               gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                              <  3:365616/141‑1 (MQ=255)

                                                                               gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                              >  2:1133669/1‑141 (MQ=255)

                                                                               gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                              <  1:605538/141‑1 (MQ=255)

                                                                                tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                             >  1:583108/1‑141 (MQ=255)

                                                                                   gAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTg                                                          <  1:513004/141‑1 (MQ=255)

                                                                                       gtgattCGCTATCTGAGTCTGAGTCGCTATCTTAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTatctg                                                      <  4:161332/136‑1 (MQ=11)

                                                                                        cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctga                                                     >  3:68404/1‑141 (MQ=255)

                                                                                           gtCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                  <  2:232150/140‑1 (MQ=255)

                                                                                           aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTGTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTgtctgtgtg                                                  >  4:233500/1‑140 (MQ=255)

                                                                                           aTCGCTATCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                  <  4:391567/141‑1 (MQ=255)

                                                                                              gCTATCTGAGTCTGAGTCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCTCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCACTATCTGAGTCTGAGTCGCTgtctgagtctga                                               <  2:331320/141‑1 (MQ=11)

                                                                                               cTATCTGATTCTGAGTCGCTTTCTGAATCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                              <  2:922765/141‑1 (MQ=255)

                                                                                                  tctgagtctgagTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                           <  2:1049312/141‑1 (MQ=255)

                                                                                                  tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                           >  2:1165177/1‑141 (MQ=255)

                                                                                                     gaatctgaGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                        <  2:484579/141‑1 (MQ=255)

                                                                                                       atctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                      <  1:94026/141‑1 (MQ=255)

                                                                                                        tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                     >  1:614131/1‑141 (MQ=255)

                                                                                                        tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                     >  2:923965/1‑141 (MQ=255)

                                                                                                          tgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                                   >  4:472192/1‑141 (MQ=255)

                                                                                                            aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                 >  1:1161462/1‑141 (MQ=255)

                                                                                                             gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatc                                <  1:136969/141‑1 (MQ=255)

                                                                                                             gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCGGAGTCGCTATCGGAGtctgagtcgcggtctgaatc                                >  2:736156/1‑141 (MQ=255)

                                                                                                               cGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                              >  3:488432/1‑141 (MQ=255)

                                                                                                                 cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                            <  4:232283/141‑1 (MQ=255)

                                                                                                                  tatctgagtcggaATCGCTATCTGAGTCGGAATCACTGTCGGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGtgtgagtcgctgtatgagtctgagt                           >  2:772899/3‑141 (MQ=11)

                                                                                                                    tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                         <  1:816512/141‑1 (MQ=255)

                                                                                                                    tctgagtccgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                         <  2:786710/141‑1 (MQ=255)

                                                                                                                      tgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgct                       <  3:134060/141‑1 (MQ=255)

                                                                                                                       gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                      <  1:962991/141‑1 (MQ=255)

                                                                                                                       gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                      <  1:175904/141‑1 (MQ=255)

                                                                                                                          tctgaGTCGCTATCTGAATCTGAGTCACTCTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGACTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                   <  2:1004162/141‑1 (MQ=11)

                                                                                                                           ctgaTTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctatctg                  <  2:731895/141‑1 (MQ=11)

                                                                                                                           ctgaGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGATTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctatctg                  <  2:103723/141‑1 (MQ=11)

                                                                                                                            tgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctga                 <  3:62364/141‑1 (MQ=255)

                                                                                                                             gaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCAGTCTGAGtctgagtcgctgtctgaatctgaatcgctgtctgac                >  4:470418/1‑140 (MQ=255)

                                                                                                                               aTCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc              <  2:535785/141‑1 (MQ=255)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc              >  1:854619/1‑141 (MQ=255)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc              >  2:279539/1‑141 (MQ=255)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc              >  1:942217/1‑141 (MQ=255)

                                                                                                                                 cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGGTGTCTGAGTCTGcgtcggtgtgggaatctgagtcgctctctgagtctg            >  2:823320/1‑141 (MQ=11)

                                                                                                                                  gCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATATGAATCTGAGTCGATGTCTGAGTCTGAgtcgctgtctgaatctgagtcgctgtctgaatctga           <  1:175012/141‑1 (MQ=255)

                                                                                                                                   cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa          <  2:268641/141‑1 (MQ=255)

                                                                                                                                         gaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGGCTGAGTCGCTGTCTGAGTCTGGAGCGCGATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg    <  3:298070/141‑1 (MQ=255)

                                                                                                                                          aatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCCGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgt   <  3:299385/141‑1 (MQ=255)

                                                                                                                                           atctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtc  >  3:259506/1‑141 (MQ=255)

AATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTC  >  USA300TCH1516_ALE/2774842‑2775121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`