BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,775,041	A→G	6.3%	D715D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,775,041	0	A	G	6.3%	452.4 / 6.9	174	D715D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (75/83); new base G (3/9); total (78/96)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

GTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTC  >  USA300TCH1516_ALE/2774903‑2775181

gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                            <  1:266069/141‑1 (MQ=255)

gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                            >  1:727120/1‑141 (MQ=255)

 tctgaatctgaaTCGCTGTCTGAGTCCGACTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCtgaatctgaatca                                                                                                                                           <  4:330366/141‑2 (MQ=11)

      atctgagtcGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                      <  2:455564/141‑1 (MQ=11)

      atctgaatcGCTGTCTGTGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTTAGTCTGAGTCGCTATCTGAATCTGAATCGCCATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                      <  2:543521/141‑1 (MQ=14)

      atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                      <  4:281575/141‑1 (MQ=255)

      atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                      >  1:969788/1‑141 (MQ=255)

      atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                      <  1:131517/141‑1 (MQ=255)

      atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                      <  1:866136/141‑1 (MQ=255)

        ctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg                                                                                                                                    <  4:181926/141‑1 (MQ=255)

         tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                                                                   <  2:1130108/141‑1 (MQ=255)

          gaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                                  >  3:417728/1‑141 (MQ=255)

           aatcGCTGTCTGGGTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                 <  2:765618/141‑1 (MQ=14)

            ctcactttCTGAGTCCGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                <  2:501102/134‑1 (MQ=14)

                  gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                          <  4:131443/141‑1 (MQ=255)

                  gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                          <  1:605538/141‑1 (MQ=255)

                   tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                         >  1:583108/1‑141 (MQ=255)

                          ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                                                                                  <  1:523966/141‑1 (MQ=255)

                           cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTatctga                                                                                                                 >  1:785966/1‑141 (MQ=255)

                           cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctga                                                                                                                 >  3:68404/1‑141 (MQ=255)

                              aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTGTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTgtctgtgtg                                                                                                              >  4:233500/1‑140 (MQ=255)

                              aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                              >  2:725095/1‑141 (MQ=255)

                                 gCTATCTGAGTCTGAGTCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCTCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCACTATCTGAGTCTGAGTCGCTgtctgagtctga                                                                                                           <  2:331320/141‑1 (MQ=11)

                                  ctgtctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                          <  2:621943/138‑1 (MQ=255)

                                  cTATCTGATTCTGAGTCGCTTTCTGAATCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                          <  2:922765/141‑1 (MQ=255)

                                     tctgagtctgagTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                       <  2:1049312/141‑1 (MQ=255)

                                     tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                       >  2:1165177/1‑141 (MQ=255)

                                     tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                       <  1:558671/141‑1 (MQ=255)

                                     tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                       >  2:282828/1‑141 (MQ=255)

                                        gaatctgaGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                                                                                    <  2:484579/141‑1 (MQ=255)

                                          atctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                  <  3:182352/141‑1 (MQ=255)

                                          atctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                  <  1:94026/141‑1 (MQ=255)

                                           tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTCTCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                                                                                 >  2:774862/1‑141 (MQ=255)

                                              gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaa                                                                                              >  3:279387/1‑141 (MQ=255)

                                               aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                                                                             >  1:1161462/1‑141 (MQ=255)

                                                  cGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                                                                                          >  3:488432/1‑141 (MQ=255)

                                                   gCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                         <  4:474884/141‑1 (MQ=255)

                                                    cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                                                                                        >  3:142442/1‑141 (MQ=255)

                                                     tGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagt                                                                                       >  3:503938/1‑141 (MQ=255)

                                                       tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                     <  1:816512/141‑1 (MQ=255)

                                                       tctgagtccgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                     <  2:786710/141‑1 (MQ=255)

                                                         tgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgct                                                                                   <  3:134060/141‑1 (MQ=255)

                                                         tgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAAtctgaatcgctgtctgagtctgaatcgct                                                                                   <  2:984932/141‑1 (MQ=14)

                                                          gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                                  <  1:175904/141‑1 (MQ=255)

                                                             tctgaGTCGCTATCTGAATCTGAGTCACTCTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGACTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                               <  2:1004162/141‑1 (MQ=11)

                                                             tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                               >  1:1094188/1‑141 (MQ=255)

                                                              ctgaTTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctatctg                                                                              <  2:731895/141‑1 (MQ=11)

                                                              ctgaGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGATTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctatctg                                                                              <  2:103723/141‑1 (MQ=11)

                                                                gaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCAGTCTGAGtctgagtcgctgtctgaatctgaatcgctgtctgac                                                                            >  4:470418/1‑140 (MQ=255)

                                                                    cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGGTGTCTGAGTCTGcgtcggtgtgggaatctgagtcgctctctgagtctg                                                                        >  2:823320/1‑141 (MQ=11)

                                                                      cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa                                                                      <  2:268641/141‑1 (MQ=255)

                                                                            gaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                                >  1:696720/1‑141 (MQ=255)

                                                                             aatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCCGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgt                                                               <  3:299385/141‑1 (MQ=255)

                                                                               tctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtct                                                             >  3:295126/1‑141 (MQ=255)

                                                                                ctgaGTCGCTATCTGAGTCTGACTCGATGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                            <  1:433148/141‑1 (MQ=255)

                                                                                   aGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGTATCTGAATCGCTgtctgtgt                                                         >  2:457958/1‑141 (MQ=255)

                                                                                    gTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                        <  3:233500/141‑1 (MQ=255)

                                                                                    gTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                        >  1:656848/1‑141 (MQ=255)

                                                                                    gTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                        >  3:330366/1‑141 (MQ=255)

                                                                                       gCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                     >  1:190229/1‑141 (MQ=255)

                                                                                        cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                                    <  2:224186/141‑1 (MQ=255)

                                                                                          ctctgagtcttactcgcttgctgagtccgagtcgctcTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGCGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                  <  4:2430/140‑1 (MQ=14)

                                                                                          aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                  <  1:141314/141‑1 (MQ=255)

                                                                                          aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                  <  3:149538/141‑1 (MQ=255)

                                                                                            ctgagtctgagtcgctatctgagtctgaggcgctgtCTGAGTCTGATTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCAGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgc                                                <  4:466400/141‑1 (MQ=255)

                                                                                            cggagtctgggtcgctgtctgagtctgagtcgctgtctGAGTCTGAATCGCTGTCTGAATCCGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgc                                                <  2:1136255/139‑1 (MQ=14)

                                                                                             tgagtctgagtcgctgtctgagtctgagtcgctgtcTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGtctgaatctgaatcgct                                               <  1:566550/141‑1 (MQ=14)

                                                                                              gagtctgagtcgctggctgagtctgagtcgctgtctGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgcta                                              <  1:990850/141‑1 (MQ=255)

                                                                                                gtctgagtcgctgtctgagtctgagtcgctgtctgaGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatc                                            >  3:474034/1‑141 (MQ=255)

                                                                                                 tctgagtcgctgtctgagtctgagtcgctgtctgagTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGATTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAATCTGAATCGCTAtctgagtctgaatcgctatct                                           >  4:85470/1‑141 (MQ=255)

                                                                                                   tgagtcgctgtctgagtctgagtcgctgtctgagtcTGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctga                                         <  2:669823/141‑1 (MQ=255)

                                                                                                   tgagtcgcgctctgagtctgagtcgctgtctgagtcTGAATCGCTATCTGACTCTGAGTCGCTGTCTGACTCTGAGTCGCCGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgattctgaatcgctatctga                                         <  2:708279/141‑1 (MQ=17)

                                                                                                      gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                                >  2:959179/1‑115 (MQ=255)

                                                                                                      gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                                <  1:959179/115‑1 (MQ=255)

                                                                                                      gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgaatctgaatcgctatctgagtc                                      >  2:382977/1‑141 (MQ=255)

                                                                                                         gctatctgagtctgaatcactgtctgagtctgaATCGCTATCGGTATCTGAGTCACTGTCTGAGTCAGAATCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAGTCGCTAtctgagtcggaatcgctatctgagtcgga                                   <  4:109818/141‑4 (MQ=11)

                                                                                                          ctgtctgagtctgagtcgctgtctgagtctgaATCGCTatctga                                                                                                                                   >  2:1154677/1‑44 (MQ=40)

                                                                                                              ctgagtctgagtcgctgtctgaatctgaATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTTTCTGAATCTGAGTCGCTTTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgc                              >  4:71497/1‑141 (MQ=14)

                                                                                                               tgagtctgagtcgctgtctgagtctgaCTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgagtctgaatcgctatctgagtctgaatcgct                             <  2:267518/141‑1 (MQ=255)

                                                                                                                gagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgcta                            >  1:615732/1‑141 (MQ=255)

                                                                                                                  gtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCtgagtctgaatcgctatctgagtctgaatcgctatc                          <  2:547926/141‑1 (MQ=255)

                                                                                                                   tctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                          >  2:235954/1‑60 (MQ=38)

                                                                                                                   tctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                          <  1:235954/60‑1 (MQ=38)

                                                                                                                    ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGagtctgaatcgctatctgagtctgaatcgctatctg                        >  1:404589/1‑141 (MQ=255)

                                                                                                                     tgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAgtctgaatcgctatctgagtctgaatcgctatctga                       <  2:433983/141‑1 (MQ=255)

                                                                                                                      gagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGtctgaatcgctatctgagtctgaatcgctatctgag                      <  1:416569/141‑1 (MQ=255)

                                                                                                                        gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTTAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGCGTCtgaatcgctatctgagtctgaatcgctatctgagtc                    >  4:496729/1‑141 (MQ=255)

                                                                                                                        gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTTAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCtgagtcgctatctgagtctgaatcgctatctgagtc                    >  4:311006/1‑141 (MQ=255)

                                                                                                                           gctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAatcgctatctgagtctgaatcgctatctgagtctga                 >  1:681108/1‑141 (MQ=255)

                                                                                                                            ctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGATTCTGAATCACTTTCTGAGTGTGaatcgctatctgagtctgtctcgctatctgagtctgga                >  3:399139/1‑139 (MQ=17)

                                                                                                                              gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCgctatctgagtctgaatcgctatctgagtctgaatc              <  2:564326/141‑1 (MQ=255)

                                                                                                                              gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc              <  1:895525/141‑1 (MQ=255)

                                                                                                                              gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTATGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc              <  3:340896/141‑1 (MQ=255)

                                                                                                                               tctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCa             >  1:794382/1‑141 (MQ=255)

                                                                                                                                  gagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTg          >  1:1106425/1‑141 (MQ=255)

                                                                                                                                    gtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGATTCGCTATCTGAGTCGGAATCCCTGTc        >  4:384639/1‑141 (MQ=255)

                                                                                                                                    gtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGATTCTGAGTCGCTATCTGCGTCTGATTCGCAATGAGTGTCTGCATCACTGTc        >  2:870755/1‑141 (MQ=14)

                                                                                                                                          aTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                         <  2:122267/54‑1 (MQ=255)

                                                                                                                                          aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTc  >  1:739520/1‑141 (MQ=255)

                                                                                                                                          aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAATCACTGTCTGAATc  >  4:268334/1‑141 (MQ=255)

GTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTC  >  USA300TCH1516_ALE/2774903‑2775181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`