Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,854,538 T→A 5.8% intergenic (+236/+177) yflS → / ← USA300HOU_RS14625 Putative malate transporter YflS/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,854,5380TA 5.8% 582.4 / 6.1 180intergenic (+236/+177)yflS/USA300HOU_RS14625Putative malate transporter YflS/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (70/95);  new base A (2/12);  total (73/107)
Fisher's exact test for biased strand distribution p-value = 4.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

TACGTTTATCCTTTAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTCATCATGACACAAAGG  >  USA300TCH1516_ALE/2854398‑2854678
                                                                                                                                            |                                                                                                                                             
 aCGTTTATCCTTTAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATa                                                                                                                                              >  1:1170269/1‑141 (MQ=255)
  cGTTTATCCTTTAAAGCAGGGCGTGCTTCACTGAAAATTGGTATAAAGATTGAAGTCAGTACGTGACAGGAGATTAATTACATGTCTAGACTATTCCGCGGCAGGGAGTAACATTACGTATGCCCTCAGAAATCATCAGAc                                                                                                                                            >  3:504333/1‑141 (MQ=255)
      tATCCTTTAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACaaaa                                                                                                                                        <  3:502146/141‑1 (MQ=255)
        tCCTTTAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACaaaaaa                                                                                                                                      <  1:796026/141‑1 (MQ=255)
         ccTTTAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAac                                                                                                                                     <  2:1147072/141‑1 (MQ=255)
             tAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCt                                                                                                                                 >  2:154274/1‑141 (MQ=255)
             tAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCt                                                                                                                                 <  4:168975/141‑1 (MQ=255)
                aTCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAGCACCTTCg                                                                                                                              >  2:125106/1‑141 (MQ=255)
                aTCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCg                                                                                                                              <  1:1180652/141‑1 (MQ=255)
                aTCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCg                                                                                                                              <  2:533626/141‑1 (MQ=255)
                  cATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGtt                                                                                                                            <  1:809426/141‑1 (MQ=255)
                    ttGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTa                                                                                                                          >  2:1044120/1‑141 (MQ=255)
                    ttGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTa                                                                                                                          >  4:394990/1‑141 (MQ=255)
                    ttGCGTGAATAACAGAAAATATGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTAATTAACATTACGTATGCCCTCATAAAAAAAAAAAAAAAAAACACCTTCGTTTa                                                                                                                          <  2:824702/141‑1 (MQ=255)
                     tGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTaa                                                                                                                         >  2:1030013/1‑141 (MQ=255)
                       cGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAAt                                                                                                                       >  2:775373/1‑141 (MQ=255)
                           cTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCAt                                                                                                                   <  3:358900/141‑1 (MQ=255)
                           cTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCAt                                                                                                                   >  1:137445/1‑141 (MQ=255)
                           cTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCAt                                                                                                                   <  1:776087/141‑1 (MQ=255)
                           cTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTCAAATTCAt                                                                                                                   <  1:465147/141‑1 (MQ=255)
                              cACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCAtttt                                                                                                                <  1:239559/141‑1 (MQ=255)
                               aCTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTa                                                                                                               >  3:208108/1‑141 (MQ=255)
                                cTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTaa                                                                                                              <  2:581446/141‑1 (MQ=255)
                                 tGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTAATTTTAAt                                                                                                             >  4:140033/1‑141 (MQ=255)
                                   aaaaTTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTg                                                                                                           <  1:536104/141‑1 (MQ=255)
                                   aaaaTTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTg                                                                                                           <  1:354674/141‑1 (MQ=255)
                                    agatgggTATAAAGAGTGAAGTCATTACGGAAAATCACATAAAATACATTTCTATACTATTCCGCTTCTTTGCTTAACATTACGTATGCCCTCATAAAAAAAAAAAAAAAAAACACCTTCGTTTAAATTCATTTTAATTGc                                                                                                          <  2:531288/135‑1 (MQ=255)
                                       tttGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGaa                                                                                                       >  2:966037/1‑141 (MQ=255)
                                         tGTATAAAGATTTAAGTCATTACGTAACATCACATAAANTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAAtt                                                                                                     >  1:172269/1‑141 (MQ=255)
                                          gTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTc                                                                                                    <  1:665858/141‑1 (MQ=255)
                                          gTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTc                                                                                                    >  1:9386/1‑141 (MQ=255)
                                           tataAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCa                                                                                                   >  1:1188075/1‑141 (MQ=255)
                                                  aTTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAg                                                                                            >  2:805586/1‑141 (MQ=255)
                                                  aTTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAg                                                                                            <  3:59185/141‑1 (MQ=255)
                                                     tAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGc                                                                                         >  2:1111583/1‑141 (MQ=255)
                                                     tAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAAATCCTTCTTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGc                                                                                         <  1:573142/141‑1 (MQ=255)
                                                      aaGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTGCGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGtt                                                                                                                            <  2:619999/105‑1 (MQ=255)
                                                      aaGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGcc                                                                                        >  2:91769/1‑141 (MQ=255)
                                                      aaGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGGTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGcc                                                                                        >  4:405615/1‑141 (MQ=255)
                                                      aaGTCATTACGTAACATCACATAAAATACATTTATATACTATTCCGCTTCATTGATTAACATTGCGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGtt                                                                                                                            >  1:619999/1‑105 (MQ=255)
                                                       aGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCt                                                                                       >  2:688083/1‑141 (MQ=255)
                                                       aGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCt                                                                                       >  1:341830/1‑141 (MQ=255)
                                                       aGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCt                                                                                       >  2:765314/1‑141 (MQ=255)
                                                        gTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGGATTCAACGAAAGTGCCtt                                                                                      >  1:308554/1‑141 (MQ=255)
                                                         tCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTa                                                                                     <  1:1027616/141‑1 (MQ=255)
                                                               cGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCat                                                                               <  3:457503/141‑1 (MQ=255)
                                                               cGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCat                                                                               >  3:46217/1‑141 (MQ=255)
                                                                 tAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCatat                                                                             <  4:364679/141‑1 (MQ=255)
                                                                  aaCATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATAtt                                                                            <  2:959764/141‑1 (MQ=255)
                                                                  aaCATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATAtt                                                                            >  2:156626/1‑141 (MQ=255)
                                                                  aaCATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATAtt                                                                            <  1:1029001/141‑1 (MQ=255)
                                                                   aCATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATAttt                                                                           <  3:109255/141‑1 (MQ=255)
                                                                     aTCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTaa                                                                         <  1:374048/141‑1 (MQ=255)
                                                                        acaTAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTAATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGt                                                                      <  2:737998/141‑1 (MQ=255)
                                                                        acaTAAAATACATTTCTATACTATTCCGCTACATTGATTACCATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGt                                                                      >  1:632127/1‑141 (MQ=255)
                                                                          aaaaaaTACATTTCTATACTACTCCGCTTCATTAATTAACATTACGTATGCCCTCATAAAAAAAAAAAAAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGttt                                                                    <  2:989508/139‑1 (MQ=255)
                                                                           tAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATTATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTCTTTCACATTCAAGGttta                                                                   >  4:351072/1‑140 (MQ=255)
                                                                            aaaaTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATCAATCATCCTCCCACAAACACATCCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCa                                                                  <  3:377144/141‑1 (MQ=255)
                                                                            aaaaTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCa                                                                  <  4:84994/141‑1 (MQ=255)
                                                                            aaaaTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCa                                                                  <  1:982008/141‑1 (MQ=255)
                                                                              aaTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCaaa                                                                <  1:964514/141‑1 (MQ=255)
                                                                               aTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAAt                                                               >  1:770766/1‑141 (MQ=255)
                                                                               aTACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAAt                                                               >  3:237277/1‑141 (MQ=255)
                                                                                tACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAAtt                                                              <  2:970148/141‑1 (MQ=255)
                                                                                  cATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTta                                                            <  2:51166/141‑1 (MQ=255)
                                                                                  cATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTta                                                            >  2:209656/1‑141 (MQ=255)
                                                                                  cATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTta                                                            <  1:883238/141‑1 (MQ=255)
                                                                                   aTTTCTATACTATTCCGTTTCATTGATCAACATTACGTATGCCCTCATAAATCATGAAATAAAAAAACACCTTCGTTTAAATTCATTTTAAGTGCGAATTCAACGAAAGTGATTTATTTCATATTCAATGTTTTAAATTta                                                            >  1:412286/1‑141 (MQ=255)
                                                                                    tttCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTtata                                                          <  1:1111583/141‑1 (MQ=255)
                                                                                    tttCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCAACATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTtata                                                          <  4:294289/141‑1 (MQ=255)
                                                                                     ttCTATANTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTTCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATCTATAc                                                         <  2:894555/141‑1 (MQ=255)
                                                                                           aCTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGt                                                   >  3:323595/1‑141 (MQ=255)
                                                                                             tATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTGATTTCATATTTAATGTTTCAAATTTATACGTCTGTCa                                                 <  1:274208/141‑1 (MQ=255)
                                                                                              aTTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCAc                                                >  3:342715/1‑141 (MQ=255)
                                                                                                 ccGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGt                                             >  1:890374/1‑141 (MQ=255)
                                                                                                 ccGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGt                                             >  4:459593/1‑141 (MQ=255)
                                                                                                 ccGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGt                                             <  1:154927/141‑1 (MQ=255)
                                                                                                 ccGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGt                                             <  2:707226/141‑1 (MQ=255)
                                                                                                 ccGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAACTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACtct                                             <  4:494518/141‑3 (MQ=255)
                                                                                                       cATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGc                                       <  1:74604/141‑1 (MQ=255)
                                                                                                          tGATTAACATTACGTATGCCCTCATAAATCATCAAACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGcaca                                    <  2:666923/141‑1 (MQ=255)
                                                                                                           gATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTAttt                                                                                  <  1:337279/94‑1 (MQ=255)
                                                                                                           gATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGcacac                                   <  3:152331/141‑1 (MQ=255)
                                                                                                                aCATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATAcc                              <  1:161261/141‑1 (MQ=255)
                                                                                                                  aTTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTc                            >  1:521200/1‑141 (MQ=255)
                                                                                                                   ttACGTATGCCCTCATAAATCATCATACAAAAAACACATTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCa                           >  1:301409/1‑141 (MQ=255)
                                                                                                                    tACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAg                          <  3:97177/141‑1 (MQ=255)
                                                                                                                      cGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGtc                        >  2:161130/1‑141 (MQ=255)
                                                                                                                       gTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACCGTTACTGCACACATACCTCAGtct                       >  2:879174/1‑141 (MQ=255)
                                                                                                                        tATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGtctc                      >  1:402235/1‑141 (MQ=255)
                                                                                                                          tGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGca                                      >  2:292940/1‑123 (MQ=255)
                                                                                                                             ccTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCtc                 >  3:29909/1‑141 (MQ=255)
                                                                                                                                  tAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTcatcat            >  1:276976/1‑141 (MQ=255)
                                                                                                                                  tAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTcatcat            <  3:288807/141‑1 (MQ=255)
                                                                                                                                    aaTCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTCATCATGa          <  3:289667/141‑1 (MQ=255)
                                                                                                                                     atcatcATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGGTACAAATTTATACGCCTGTCACTGTTACTGCACACATACCTCAGGCTCACCTCATCATGac         >  1:949809/1‑141 (MQ=255)
                                                                                                                                      tcatcaTACAAAAAACACCTTCGTTTAAATCCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGCCTGTCACTCTTACTGCACACATACCTCAGTCTCACCTCATCATGaaa        >  4:476880/1‑139 (MQ=255)
                                                                                                                                       catcatACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTCATCATGacac       <  1:99919/141‑1 (MQ=255)
                                                                                                                                       catcatACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTATCACCTCATCATGacac       >  1:664909/1‑141 (MQ=255)
                                                                                                                                        atcagACAAAAAACCCCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTCAGCATGAcaca      <  2:48172/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TACGTTTATCCTTTAAATCATTGCGTGCTTCACTGAAAATTTGTATAAAGATTTAAGTCATTACGTAACATCACATAAAATACATTTCTATACTATTCCGCTTCATTGATTAACATTACGTATGCCCTCATAAATCATCATACAAAAAACACCTTCGTTTAAATTCATTTTAATTGCGAATTCAACGAAAGTGCCTTATTTCATATTTAATGTTTCAAATTTATACGTCTGTCACTGTTACTGCACACATACCTCAGTCTCACCTCATCATGACACAAAGG  >  USA300TCH1516_ALE/2854398‑2854678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: