BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,904	G→T	44.5%	D255Y (GAT→TAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,904	0	G	T	44.5%	197.5 / inf	280	D255Y (GAT→TAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base G (75/80); new base T (65/60); total (140/140)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 288 total aligned reads displayed.

AAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG  >  USA300TCH1516_ALE/110764‑111044

aaGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGgag                                                                                                                                              <  2:444107/141‑1 (MQ=255)

    tacAAAGTTATTGTTAAAAGGTTAAAGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTCAGTGATAGCTAAAAAAAAAAAAAAAGCGGAGATGt                                                                                                                                          <  4:203805/139‑1 (MQ=21)

     ccAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGta                                                                                                                                         <  4:196812/141‑1 (MQ=255)

       aaaGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATACAGATTTTGACTTTACCTTCGCCGCGACACAAGAAGCAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGtata                                                                                                                                       <  1:1009918/141‑1 (MQ=37)

       aaaGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGtata                                                                                                                                       <  1:908271/141‑1 (MQ=255)

         aGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGtata                                                                                                                                       >  4:477419/1‑141 (MQ=255)

         aGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAAt                                                                                                                                     >  2:564509/1‑141 (MQ=255)

          gTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATc                                                                                                                                    <  4:220045/141‑1 (MQ=255)

             aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATg                                                                                                                                 <  4:142780/141‑1 (MQ=255)

             aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg                                                                                                                                 >  1:559963/1‑141 (MQ=255)

               tGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGc                                                                                                                               >  2:736682/1‑141 (MQ=255)

                gTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGACAAAAGAAGAAA‑‑ATATATACTTTTGTGATAGCAAAAAAAAAAAAAAAAGCGGAGATGTATAATCATGGCt                                                                                                                              <  2:108691/141‑1 (MQ=21)

                gTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCt                                                                                                                              >  2:22274/1‑141 (MQ=255)

                gTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCt                                                                                                                              >  4:295973/1‑141 (MQ=255)

                gTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCt                                                                                                                              >  2:1119281/1‑141 (MQ=255)

                    aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt                                                                                                                          <  1:1125604/141‑1 (MQ=255)

                    aaaGGTTAAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAAt                                                                                                                          >  1:945061/1‑141 (MQ=255)

                      aGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCa                                                                                                                        >  3:3091/1‑141 (MQ=255)

                          tCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAat                                                                                                                    >  3:491783/1‑141 (MQ=255)

                               gAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGaa                                                                                                               <  2:619529/141‑1 (MQ=255)

                                aaTTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGaaa                                                                                                              <  3:201295/141‑1 (MQ=255)

                                aaTTTAAAAGGCTCATCAGGTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAAAAAAAAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGaaa                                                                                                              <  2:601808/141‑1 (MQ=25)

                                aaTTTAAAAGGCTCATAAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGaaa                                                                                                              <  4:64652/141‑1 (MQ=255)

                                 aTTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGAAAAAAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAaat                                                                                                             <  2:51538/141‑1 (MQ=255)

                                    tAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATg                                                                                                                                           <  1:251582/108‑1 (MQ=255)

                                     aaaaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaat                                                                                                         >  1:271078/1‑141 (MQ=255)

                                       aaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATcc                                                                                                       >  2:48928/1‑141 (MQ=255)

                                        aGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATccc                                                                                                      <  1:421783/141‑1 (MQ=255)

                                        aGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATccc                                                                                                      >  2:1013882/1‑141 (MQ=255)

                                        aGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATccc                                                                                                      >  1:857627/1‑141 (MQ=255)

                                            tcatcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATACGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaa                                                                                                  >  2:402499/1‑141 (MQ=255)

                                             catcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaa                                                                                                 <  1:87164/141‑1 (MQ=255)

                                              atcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaa                                                                                                >  1:387543/1‑141 (MQ=255)

                                              atcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaa                                                                                                >  2:359977/1‑141 (MQ=255)

                                               tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa                                                                                               >  2:860619/1‑141 (MQ=255)

                                                  gTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCACGGAAAAagag                                                                                            >  2:14322/1‑141 (MQ=255)

                                                     ggATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑CTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGt                                                                                         >  3:413627/1‑141 (MQ=255)

                                                     ggATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCGGGAAAAagagaga                                                                                         >  1:688605/1‑140 (MQ=255)

                                                           aaaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaa                                                                                   <  1:661044/141‑1 (MQ=255)

                                                           aaaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaa                                                                                   <  1:795956/141‑1 (MQ=255)

                                                            aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  >  1:202144/1‑141 (MQ=255)

                                                            aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  >  4:401447/1‑141 (MQ=255)

                                                            aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  >  4:242290/1‑141 (MQ=255)

                                                            aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  <  1:787830/141‑1 (MQ=255)

                                                              gATATTGAATTTACGTTTGTATAGAAAAAAGAATAAA‑‑ATATATACTGTAGTTATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATg                                                                                <  1:1005450/141‑1 (MQ=39)

                                                              gATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATg                                                                                <  1:309751/141‑1 (MQ=255)

                                                              aatatTAAATTTACGTGTGTATAGAAAAAAGAAGAAA‑‑ATATATAATTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATg                                                                                <  4:374931/140‑1 (MQ=255)

                                                                 atTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAAAAAAAAAAAGAAAAAGAGAGTGATGAAATGATa                                                                             <  2:257969/141‑1 (MQ=37)

                                                                 atTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATa                                                                             <  1:437604/141‑1 (MQ=255)

                                                                  ttGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATaa                                                                            >  3:174558/1‑141 (MQ=255)

                                                                   tGAATTTACGAATGAAGAAAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATaaa                                                                           <  2:813672/141‑1 (MQ=255)

                                                                    gAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATaaaa                                                                          >  4:317325/1‑141 (MQ=255)

                                                                     aaTTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCa                                                                                                                                   <  2:244488/83‑1 (MQ=255)

                                                                     aaTTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAAc                                                                         >  2:120345/1‑141 (MQ=255)

                                                                          aCGTTTTTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATTGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGta                                                                    <  2:1139168/141‑1 (MQ=255)

                                                                          aCGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGta                                                                    <  2:1171086/141‑1 (MQ=255)

                                                                          aCGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGta                                                                    >  1:879339/1‑141 (MQ=255)

                                                                           cGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaa                                                                   >  1:575665/1‑141 (MQ=255)

                                                                                 tAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTaaataaat                                                             >  1:933298/1‑141 (MQ=255)

                                                                                 tAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTaaataaat                                                             >  2:130874/1‑141 (MQ=255)

                                                                                 tAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTaaataaat                                                             <  1:1034506/141‑1 (MQ=255)

                                                                                   gagaAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTa                                                           <  2:965075/141‑1 (MQ=255)

                                                                                         aaaGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCtt                                                     >  1:965821/1‑141 (MQ=255)

                                                                                          aagaagAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTg                                                    >  2:27459/1‑141 (MQ=255)

                                                                                          aagaagAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTg                                                    >  3:505858/1‑141 (MQ=255)

                                                                                           agaagaAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTgg                                                   >  3:256490/1‑141 (MQ=255)

                                                                                            gaagaaA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGt                                                  <  1:143325/141‑1 (MQ=255)

                                                                                            gaagaaA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGt                                                  >  1:211487/1‑141 (MQ=255)

                                                                                            gaagaaA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGt                                                  <  1:474181/141‑1 (MQ=255)

                                                                                             aagaaA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTa                                                 >  2:247748/1‑141 (MQ=255)

                                                                                              agaaA‑‑ATATATACTTTAGTGATAGCTTAGATAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAt                                                <  4:226549/141‑1 (MQ=255)

                                                                                                 aa‑‑aTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAg                                             >  1:695444/1‑141 (MQ=255)

                                                                                                 aa‑‑aTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATAAGTGCTTGGTATTAg                                             >  2:1060142/1‑141 (MQ=255)

                                                                                                     atatatACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtc                                           <  3:137369/141‑1 (MQ=255)

                                                                                                       atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  2:679704/141‑1 (MQ=255)

                                                                                                       atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  3:154072/141‑1 (MQ=255)

                                                                                                       atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         >  3:462128/1‑141 (MQ=255)

                                                                                                            cTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGtt                                    >  3:66992/1‑141 (MQ=255)

                                                                                                              ttAGTGANAGCTTAGATTATAAAAAAAGCGGAGATGAATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTTGTTTGTGTTACTAGTCTTCTGtttt                                  <  2:898244/141‑1 (MQ=255)

                                                                                                                      aGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCAtt                          >  2:1078239/1‑141 (MQ=255)

                                                                                                                         ttAGATTATAAAAAAAGCGGAGATGTAGAAGCAGTTTACTAATAGAATATTTAATCAATCCCGTAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGt                       <  1:531658/141‑1 (MQ=255)

                                                                                                                          tAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTa                      >  3:346551/1‑141 (MQ=255)

                                                                                                                          tAGATTATAAAAAAAGCGGAGATGTAGAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTa                      <  2:945138/141‑1 (MQ=255)

                                                                                                                             aTTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCa                   <  3:273985/141‑1 (MQ=255)

                                                                                                                             aTTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCa                   <  1:740857/141‑1 (MQ=255)

                                                                                                                             aTTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCa                   <  3:84477/141‑1 (MQ=255)

                                                                                                                                 taAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGGCATTAGTATCActgc               >  1:376475/1‑141 (MQ=255)

                                                                                                                                  aaaaaaaGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgct              >  3:193072/1‑141 (MQ=255)

                                                                                                                                  aaaaaaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgct              >  2:1029095/1‑141 (MQ=255)

                                                                                                                                  aaaaaaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgct              >  1:810610/1‑141 (MQ=255)

                                                                                                                                      aaaGCGGATATGTATAATCATTGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGTTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGtt          <  2:992176/141‑1 (MQ=255)

                                                                                                                                      aaaGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGtt          <  4:435389/141‑1 (MQ=255)

                                                                                                                                       aaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtg         >  1:777842/1‑141 (MQ=255)

                                                                                                                                        aGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtgt        >  4:284734/1‑141 (MQ=255)

                                                                                                                                        aGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtgt        <  1:1099759/141‑1 (MQ=255)

                                                                                                                                        aGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtgt        >  2:157674/1‑141 (MQ=255)

                                                                                                                                          cGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTgg      >  1:15183/1‑141 (MQ=255)

                                                                                                                                           ggAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTggg     >  1:1043052/1‑140 (MQ=255)

                                                                                                                                             agaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtct                                          <  2:934842/102‑1 (MQ=255)

                                                                                                                                              gaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATg  <  4:3091/141‑1 (MQ=255)

AAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAA‑‑ATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG  >  USA300TCH1516_ALE/110764‑111044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`