| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,807,163 | C→T | 6.8% | G487R (GGG→AGG) | asp2 ← | Accessory Sec system protein Asp2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,807,163 | 0 | C | T | 6.8% | 200.0 / 3.3 | 73 | G487R (GGG→AGG) | asp2 | Accessory Sec system protein Asp2 |
| Reads supporting (aligned to +/- strand): ref base C (33/33); new base T (2/3); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01 | |||||||||||
GTTTGTGAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACAATGATTTCTTTTGA > USA300TCH1516_ALE/2807023‑2807299 | gTTTGTGAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCTTCTTTATTTGGccc > 1:355164/1‑141 (MQ=255) tgtcAAATATAATTCCAAGGAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAgg < 1:406778/141‑1 (MQ=255) tactaaaTAAGATTCCAAGTAACTTGAAATGTTTTGTATTTTAGCAAACTGAACTCTCCCAAACGTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAgg < 1:567997/137‑1 (MQ=255) gtgAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTCTGTCGCCCAGGa > 1:813091/1‑141 (MQ=255) gtgAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTAAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACAAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGa > 2:620152/1‑141 (MQ=255) tgAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCGACGaa > 1:61517/1‑141 (MQ=255) tgAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCAACATTACGTCGCCCaagca > 2:118645/1‑137 (MQ=255) gAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCACCATTATGTCGCCCAGGAAt < 1:545677/141‑1 (MQ=255) gAAATATAATCCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTAAATACTTTTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAAt > 1:607963/1‑141 (MQ=255) tataATTCCAAGTAACTTTAAATGTTTTGTTTTTCTGCATGTTGAACTCTCCCAAACTTGTCTTCCAAATTAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTg > 2:234033/1‑141 (MQ=255) aaTTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATATTGAACGCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAAGGGGAGGTGAATCATCATTATGTCGCCACGAAATACTGCGa > 2:457934/1‑141 (MQ=255) tttCTAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGAt < 2:247592/140‑1 (MQ=255) tCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCGTTATGTCGCCCAGGACTACTGCGATtc > 1:392619/1‑141 (MQ=255) cAAGTACCTTTAATTGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAATATAATGTTGTAAAAATTAACAAACCAACTTGCTATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATtcat < 2:27677/141‑1 (MQ=255) aaGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATtcatc < 2:443037/141‑1 (MQ=255) gtgaccttAAATGTTTTCTATTTCAGCATGTTGAACTCTCCCACACTTGTCTTCCAAAAAAAAAAAATAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCAc < 2:744907/135‑1 (MQ=255) gTAACTTTAATTGTTTTGTTTTTCAGCATGATGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCAc < 2:668072/141‑1 (MQ=255) gTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCACAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCAc > 2:139636/1‑141 (MQ=255) gTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCAc < 1:693890/141‑1 (MQ=255) tttAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTcttg > 1:580358/1‑141 (MQ=255) aaTGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAACACTGCGATTCATCACTcttgcttg < 1:533739/141‑1 (MQ=255) tgttttgtttTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGgt > 1:545428/1‑141 (MQ=255) tttgttttTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGtgtg < 2:179232/141‑1 (MQ=255) gttttTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTg < 2:418316/141‑1 (MQ=255) gttttTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTg < 1:624318/141‑1 (MQ=255) tttttCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCTAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGt > 2:177457/1‑141 (MQ=255) tttttCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCTAGGAATACTGCGATCCATCACTCTTGCTTGGTGTGCTGt > 2:320870/1‑141 (MQ=255) ttttCAGCATTTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAGAATTAACAAACCAACTTGCAATGGTAGGTGAATAATCATTATGTCGCCCAGATATACTTCGATTTATCACTTTTGCTTGGTTTGCTGTc > 2:91826/1‑141 (MQ=255) ttttCAGCATGTTTAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTc < 2:265290/141‑1 (MQ=255) ttttCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTACTGTc < 2:731612/141‑1 (MQ=255) tttgCATCATGTTGATCTCCCCCAACCTCGTCTGACAACATAAGGTTGTACAAATTAACAAACCAACTTGCAATGGTATGGGACTCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTc < 2:91629/141‑1 (MQ=255) ttCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACTAACTTGCAATGGTTGGTGGATCATTATTATGTTGAGCAGGGATTGTGGGATTCATCACTTTTGCTTGGTTTGCTGTCat > 2:281556/1‑140 (MQ=255) tCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAAt > 2:220216/1‑141 (MQ=255) tCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGCCCTGTCAAt > 2:571213/1‑141 (MQ=255) gTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTa < 1:821332/141‑1 (MQ=255) aaCTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAg < 1:95429/141‑1 (MQ=255) ctctCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCCGGAATACTGCGAGTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTaaaaact > 1:784046/1‑136 (MQ=255) ctctCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑ct > 2:130079/1‑141 (MQ=255) tctcCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAAAAAACCAACGTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑ctc < 1:782104/141‑1 (MQ=255) ttGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCa < 2:437225/141‑1 (MQ=255) tgttatCCAAAATAATGTGGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCTATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCAt < 2:463598/136‑1 (MQ=255) ttCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTgg > 1:357852/1‑141 (MQ=255) ttCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTgg < 2:740293/141‑1 (MQ=255) tCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTAGGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAAGACAGGTAATAG‑CTCTTGAAATGCATGTGGa > 1:379303/1‑141 (MQ=255) aaaaTAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCa > 2:373376/1‑141 (MQ=255) aaaaTAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCa < 1:837952/141‑1 (MQ=255) aatGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATAATTATGCAGCTCACGAATACTGCGATTCATCACCCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑ACCTTGCACTGCAGGTGGATCATaacc > 2:346339/1‑139 (MQ=255) atGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAatca > 2:54068/1‑141 (MQ=255) atGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATCTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCCTAatca < 2:526287/141‑1 (MQ=255) tgttgtAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAATTGCATGTGGATCATAatcgt > 2:638333/1‑139 (MQ=255) tgttgtAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAatcat < 1:599849/141‑1 (MQ=255) ttgtAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAAtcatca > 1:380586/1‑141 (MQ=255) ttgtAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAAtcatca < 1:93331/141‑1 (MQ=255) ttgtAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAAtcatca > 2:192151/1‑141 (MQ=255) ttgtAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGAAATAG‑CTCTTGAAATGCATGTGGATCATAAtcatca > 2:65104/1‑141 (MQ=255) aaaaTTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTg < 2:399648/141‑1 (MQ=255) aaaTTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTCAAATTACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGc > 1:649372/1‑141 (MQ=255) aaTTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATATAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCa < 2:256373/141‑1 (MQ=255) aTTAACAAACCAACATGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTCGTGTGCTGTCAAGACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCat > 2:572695/1‑141 (MQ=255) aaCCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCt > 1:572277/1‑141 (MQ=255) ttGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGGTCATAATCGACATGTTGCATATATGCTTTTGCaa > 1:189433/1‑141 (MQ=255) tGCAATGGTAGGTGAATCATCATTATGTCGCCTAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCaaa < 1:177457/141‑1 (MQ=255) tGCAATGGTAGGTGAATCATCATTATGTCGCCTAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGGCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCaaa < 1:320870/141‑1 (MQ=255) aaTGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGATTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAAc < 2:297948/141‑1 (MQ=255) atcgTAGGTGAATAATTATTCTGTCGCCCAGGACTATTGCGATTTCTCGCTTTTCCTTGGTGGTCGGGCAATAAAGGTCTCAGACTGTT‑TCTTGCATTTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACa < 2:804179/138‑1 (MQ=255) tGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGCCACGCATGCGGATCATAATAATCATGTTGCATCTATGCTACGGCAAAAACAg > 1:798979/1‑141 (MQ=255) tGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAg > 1:268742/1‑141 (MQ=255) aGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGATtg > 1:558620/1‑141 (MQ=255) aaTCATCATTATGTCGCCCAGGAACACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAATACAGATTGTGACa > 1:611474/1‑141 (MQ=255) atcatcATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACaa < 1:14303/141‑1 (MQ=255) atcatTATGTGGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACAATGa < 2:255320/141‑1 (MQ=255) atTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACAATGAttt > 2:194817/1‑141 (MQ=255) cGCCTAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACAATGATTTCTTTTGa < 1:384780/141‑1 (MQ=255) cGCACAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACAATGATTTCTTTTGa < 1:255370/141‑1 (MQ=255) | GTTTGTGAAATATAATTCCAAGTAACTTTAAATGTTTTGTTTTTCAGCATGTTGAACTCTCCCAAACTTGTCTTCCAAAATAATGTTGTAAAAATTAACAAACCAACTTGCAATGGTAGGTGAATCATCATTATGTCGCCCAGGAATACTGCGATTCATCACTCTTGCTTGGTGTGCTGTCAATACAGGTAATAG‑CTCTTGAAATGCATGTGGATCATAATCATCATGTTGCATATATGCTATGGCAAAAACAGTTTGTGACAATGATTTCTTTTGA > USA300TCH1516_ALE/2807023‑2807299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |