Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 38,412 A→G 7.8% A200A (GCA→GCG)  ugpQ_1 → Glycerophosphodiester phosphodiesterase, cytoplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE38,4120AG 7.8% 204.5 / 4.6 70A200A (GCA→GCG) ugpQ_1Glycerophosphodiester phosphodiesterase, cytoplasmic
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base G (3/3);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGCATTTGTCT  >  USA300TCH1516_ALE/38272‑38550
                                                                                                                                            |                                                                                                                                          
aaaCTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCa                                                                                                                                            >  1:486658/1‑141 (MQ=255)
 aaCTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCaa                                                                                                                                           >  2:458898/1‑141 (MQ=255)
  aCTTGCAGAAGAAATCATGGCACAATATAACAGAGCAGTTATATTCCATACAACGTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCaaa                                                                                                                                          >  1:544795/1‑141 (MQ=255)
  aCTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCaaa                                                                                                                                          >  1:105415/1‑141 (MQ=255)
  aCTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCaaa                                                                                                                                          >  2:810860/1‑141 (MQ=255)
  aCTTGCAGAAGAAATCATGCAACAATATAACAGAGCAGTTATATTCCATACAAATTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTCAAGaaaa                                                                                                                                          >  2:118503/1‑141 (MQ=255)
   cTTGCAGAAGAAATCATGCCACAATATAGCAGAGCAGGTATATTCCATACAACGTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACCAAAGAaaaa                                                                                                                                         >  2:830184/1‑141 (MQ=255)
   cTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCaaaa                                                                                                                                         <  1:278738/141‑1 (MQ=255)
     tGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGt                                                                                                                                       <  1:8179/141‑1 (MQ=255)
     tGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGt                                                                                                                                       <  1:774116/141‑1 (MQ=255)
     tGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGt                                                                                                                                       <  1:232192/141‑1 (MQ=255)
     tGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGt                                                                                                                                       <  1:690385/141‑1 (MQ=255)
     tGCAGAAGAAAGCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGt                                                                                                                                       <  1:154779/141‑1 (MQ=255)
       cAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTaa                                                                                                                                     >  2:719167/1‑141 (MQ=255)
       cAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACGTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTaa                                                                                                                                     <  1:383130/141‑1 (MQ=255)
                tCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTaa                                                                                                                            <  1:729754/141‑1 (MQ=255)
                 cATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTaaa                                                                                                                           <  1:410419/141‑1 (MQ=255)
                  aTGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGATAGATTAGATAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTaaaa                                                                                                                          >  1:784951/1‑141 (MQ=255)
                   cgCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAaag                                                                                                                         <  2:94644/140‑1 (MQ=255)
                        caATATAACAGAGCGGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCg                                                                                                                    >  2:569693/1‑141 (MQ=255)
                          atatAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCggg                                                                                                                  <  1:458898/141‑1 (MQ=255)
                                    gCAGTTATATTCCATACAACTTCGTTTCGTTAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAACTGGCCAAAGAAGCGGGTTATGAATTg                                                                                                        <  2:809638/141‑1 (MQ=255)
                                       gTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGGGGGTTATGAATTGAAc                                                                                                     >  1:422638/1‑141 (MQ=255)
                                        ttATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCGAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACg                                                                                                    >  2:492513/1‑141 (MQ=255)
                                        ttATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACg                                                                                                    <  1:815011/141‑1 (MQ=255)
                                         tataTTCCATACAATTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGt                                                                                                   <  1:661482/141‑1 (MQ=255)
                                          atatTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTa                                                                                                  >  2:693012/1‑141 (MQ=255)
                                           tatTCCATACAACTTCGTTTCGTGAAGACTGGAGAGCACTTGTAGATTAATGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTAt                                                                                                 >  2:404699/1‑141 (MQ=255)
                                            atTCCATACAATCTGTTTTCGTGAAGACTGTAGAGCACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGTCGAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATg                                                                                                <  1:504246/141‑1 (MQ=255)
                                            atTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCGAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATg                                                                                                <  2:195520/141‑1 (MQ=255)
                                            atTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATg                                                                                                >  2:51773/1‑141 (MQ=255)
                                            atTCCATACAACTTCGTTGCGTGAAGACTGGAGAACACTTTGAGATTACTGTAATACTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATg                                                                                                <  1:10300/141‑1 (MQ=255)
                                            agtCCAAACAACTTCGTTTCGGGAAGACTGGAGAACACTCTTAGACTAATGTAATCCTAACAAAGTAAACACTGAATATTTCAATCTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATg                                                                                                <  2:91011/139‑1 (MQ=255)
                                            aatCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGAAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTAAAAAAAAAAAAAAAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATg                                                                                                <  2:430237/139‑1 (MQ=255)
                                                      aCTTCGTTTCGTGAAGCGTGGTGTACGCTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATAGACTGTaaac                                                                                      >  2:595000/1‑141 (MQ=255)
                                                         tcgtttcgtGAAGACTGGAGAACCCTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGGAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTaaacaaa                                                                                   <  2:486658/141‑1 (MQ=255)
                                                          cgtttcgtGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGGCGGTaaacaaac                                                                                  >  1:88066/1‑141 (MQ=255)
                                                            tttcgtGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCa                                                                                >  2:583942/1‑141 (MQ=255)
                                                            tttcgtGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAAAAAACCa                                                                                >  2:519950/1‑141 (MQ=255)
                                                               cgtGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCa                                                                             >  1:776561/1‑141 (MQ=255)
                                                                    aGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGGAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGc                                                                        >  1:368078/1‑141 (MQ=255)
                                                                     gACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCa                                                                       <  1:356687/141‑1 (MQ=255)
                                                                     gACTGGAGAACACTTTTAGATTACTGTAATGCGAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTAGGAATTGAACGTATGGACTGTAAACAAACCAGCAAGTGCa                                                                       >  2:732989/1‑141 (MQ=255)
                                                                     gACTGGAGAACACTTTTAGATTACTGGAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCa                                                                       <  1:553295/141‑1 (MQ=255)
                                                                        tGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGACGCGGGTGATGAAGTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAAc                                                                    >  1:155458/1‑141 (MQ=255)
                                                                        tGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCAAGTGCAAAc                                                                    >  2:127260/1‑141 (MQ=255)
                                                                              acacTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACtt                                                              <  2:13471/141‑1 (MQ=255)
                                                                                    ttATATTACTTTAATGCAAAAGTAGAGCACATTTAAGATGCTAAACTTAATAAAGCAAAAGTAAAAATGTAAAAAGAAGCGGGTTATGAATGGAACGTATGAACTGTAAACAACCCATCACGTGCAAACCAACTTGCTAAt                                                        <  2:133437/141‑1 (MQ=255)
                                                                                        aTTACTCTAATGCTAAAATACTAAACACTAAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGTTTATCAATTGAACGTATTGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTggg                                                    <  2:785078/141‑1 (MQ=255)
                                                                                            cTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTGGCTAATTAGGgtgt                                                >  2:574544/1‑141 (MQ=255)
                                                                                              ttAATGCTAAAATAGTAAACACTGAAGATGCCAAACCTACTAAAGCAAAAGTAAAAATGATAAAAGAAGCGGGTTATGAATTGAACGTATGAACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTg                                              <  2:746421/140‑1 (MQ=255)
                                                                                                   gCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATCGGGGAGTTGATGGt                                         >  1:380957/1‑141 (MQ=255)
                                                                                                      aaaaTAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATc                                      <  2:544795/141‑1 (MQ=255)
                                                                                                      aaaaTAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATc                                      <  2:105415/141‑1 (MQ=255)
                                                                                                      aaaaTAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATc                                      <  1:810860/141‑1 (MQ=255)
                                                                                                            gTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACa                                >  1:343882/1‑141 (MQ=255)
                                                                                                            gTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACa                                >  2:793655/1‑141 (MQ=255)
                                                                                                               aaCACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGAc                             >  1:789010/1‑141 (MQ=255)
                                                                                                                acacTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGTTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGGTGATGGTATCTTTACAGACa                            >  1:226311/1‑141 (MQ=255)
                                                                                                                acacTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAAAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACa                            >  2:740672/1‑141 (MQ=255)
                                                                                                                   cTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATg                         <  1:569693/141‑1 (MQ=255)
                                                                                                                     gAAGATGCCAAACTTACTAAAGCGAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCa                       >  2:74424/1‑141 (MQ=255)
                                                                                                                     gAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGCATTGAACGTATGGACTGTAAACAAACCCGCACGTGCACACCAACTTGCTAATTGGGGAGTTGGTGGTATCTTTCCAGAccctgcc                       >  1:10419/1‑135 (MQ=255)
                                                                                                                     gAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCa                       >  2:82146/1‑141 (MQ=255)
                                                                                                                       aGATGCCAAACTTACTCAAGCGAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGa                     >  1:430754/1‑141 (MQ=255)
                                                                                                                       aGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGa                     >  1:768160/1‑141 (MQ=255)
                                                                                                                        gATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGAt                    <  1:351224/141‑1 (MQ=255)
                                                                                                                        gATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGATGAGCGTATCGTTACAGCCAATGCAGAt                    >  2:130472/1‑141 (MQ=255)
                                                                                                                        gATGCAAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGAt                    <  1:269896/141‑1 (MQ=255)
                                                                                                                        aaTGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGAt                    <  1:690502/140‑1 (MQ=255)
                                                                                                                          tGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACCGCCAATGCAGATaa                  >  1:220170/1‑141 (MQ=255)
                                                                                                                                  ttACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGc          <  2:1217/141‑1 (MQ=255)
                                                                                                                                    aCTAAAGCGAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGTGGAGGTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGCAt        <  2:301829/141‑1 (MQ=255)
                                                                                                                                    aCTAAAGCGAAAGTAAAAAGGGTAAAAGAAGCGGGTTATGAATTTAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGCAt        <  2:669712/141‑1 (MQ=255)
                                                                                                                                     cTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGGAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTCGATGGTATCTTTACAGACAATGCAGCAAAAATGGTGCCtt       >  2:471702/1‑141 (MQ=255)
                                                                                                                                      tAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGGGCAttt      >  2:353182/1‑141 (MQ=255)
                                                                                                                                       aaaGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGCATTTg     >  1:239768/1‑141 (MQ=255)
                                                                                                                                          gCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGCATTTGtct  >  1:157132/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AAACTTGCAGAAGAAATCATGCCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACACTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCAAAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCAGCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGATAAAATGGTGCATTTGTCT  >  USA300TCH1516_ALE/38272‑38550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑