BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,512	A→G	22.3%	S194S (TCA→TCG)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,512	0	A	G	22.3%	198.5 / 53.3	107	S194S (TCA→TCG)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (43/40); new base G (11/13); total (54/53)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 107 total aligned reads displayed.

TACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAA  >  USA300TCH1516_ALE/111374‑111648

tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                                                         >  1:27571/1‑141 (MQ=255)

tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGc                                                                                                                                         >  2:685986/1‑141 (MQ=255)

 acagAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCt                                                                                                                                        <  1:101373/138‑1 (MQ=255)

 aatGAGGAAGTTAAAGAAGAAAACAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                                                        <  2:396485/139‑1 (MQ=255)

  cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                       >  2:403226/1‑141 (MQ=255)

     aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                    <  2:831036/141‑1 (MQ=255)

      gtaaGTTAAAAAATAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                   <  2:158953/139‑1 (MQ=255)

      ggAAGTTAAAGAACAAAACATAAAAAAAGAAATCGAAATCTTTAAGTTTTTTGCGCAATTTAGCAATTTTAAAGATTTAATGAACTATAAAGATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTAGTCGGCTAAata                                                                                                                                   <  1:41754/141‑1 (MQ=18)

         aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTCATTTTTGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGTTTCCGAGTTATTCAGCTCAatataa                                                                                                                                >  2:263393/1‑139 (MQ=18)

         aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                >  1:180143/1‑141 (MQ=255)

             aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                            >  2:66775/1‑141 (MQ=255)

             aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTa                                                                                                                            >  2:773588/1‑141 (MQ=255)

             aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGGTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGGATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTGACCTCAATATCAATTa                                                                                                                            >  2:773538/1‑141 (MQ=255)

              aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTACTGAATTATAAAGATGGAGATATATCATATAATCCAGCGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                           >  1:195211/1‑141 (MQ=255)

              aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                           <  2:472306/141‑1 (MQ=255)

              aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                           <  1:776001/141‑1 (MQ=255)

              aagaaaAAAACAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                           <  2:451054/141‑1 (MQ=255)

               agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                          >  1:738810/1‑141 (MQ=255)

                gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                         >  1:247335/1‑141 (MQ=255)

                gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                         >  1:100592/1‑141 (MQ=255)

                aaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTCGGCTAAATATCAATTAACt                                                                                                                         <  1:660996/140‑1 (MQ=255)

                  agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGATTTATTCGGCTAAATATCAATTAACTaa                                                                                                                       <  2:180143/141‑1 (MQ=255)

                   gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCGGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                      >  1:239577/1‑141 (MQ=255)

                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTCGGATAAATATCAATTAACTAatgat                                                                                                                   >  1:400192/1‑141 (MQ=255)

                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                   <  1:100811/141‑1 (MQ=255)

                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                   <  1:95953/141‑1 (MQ=255)

                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATGTAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                  >  2:570541/1‑141 (MQ=255)

                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                  >  1:526914/1‑141 (MQ=255)

                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                  >  1:721437/1‑141 (MQ=255)

                          tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAATTAACTAATGATGAt‑t                                                                                                              >  2:756717/1‑141 (MQ=255)

                           aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑ta                                                                                                             >  2:401272/1‑141 (MQ=255)

                            aacaaaCAAATCGAAAACTTTAAGTTTCTTGCTCAAAATCGCAACTTATAAGATTTAATGAATTATAAAGATGGCGATATATCATATAATTCAGAGGTGCCGAGCTATTCGGCTAAATATCAATTAACTAATGATGAT‑tat                                                                                                            <  2:122781/138‑1 (MQ=14)

                            aaaaaaGAAATCGAAAATTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑tat                                                                                                            <  2:18849/141‑1 (MQ=255)

                              aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATaa                                                                                                          <  2:468740/141‑1 (MQ=255)

                                  gAAATCTAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTa                                                                                                      <  2:783717/141‑1 (MQ=255)

                                  gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTa                                                                                                      >  1:519246/1‑141 (MQ=255)

                                   aaaTCGTAATCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaa                                                                                                     <  1:680882/141‑1 (MQ=255)

                                     aTCGAATACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaaaa                                                                                                   <  2:818689/141‑1 (MQ=255)

                                     aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaaaa                                                                                                   <  1:536112/141‑1 (MQ=255)

                                     aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaaaa                                                                                                   <  1:124134/141‑1 (MQ=255)

                                      ttgAAAAGTTTAAGTTTTTGGCGCGATATGGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATATTCCAGAGGTGCCGAGTTATTCATCTCAATATCAATTAACTAATGATGAT‑TATAATGTAacac                                                                                                  <  2:423474/139‑1 (MQ=18)

                                      tCGGAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                  <  2:657947/141‑1 (MQ=255)

                                      tCGAAAACTTTAAGTTTTTTTTTTTTTTTAGAAATTATAAAGGTTTAATGAATTATAAAGATGGAGTTGTGTCATATAATCTATAGTTGCCGAGTTATTAAGCTCAATATCATTTAAATATTGTTGAT‑TTTATTGTataat                                                                                                  >  2:221110/1‑140 (MQ=255)

                                      tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                  <  2:118504/141‑1 (MQ=255)

                                      tCGAAAACTTTAAGTTTTTAGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                  >  1:502009/1‑141 (MQ=255)

                                      tCGAAAACTTTAAGTCTTTCGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                  >  1:624731/1‑141 (MQ=255)

                                         aaaaCTTTAAGTTTTTTGCGGAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAAt                                                                                               <  2:647745/141‑1 (MQ=255)

                                         aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAAt                                                                                               <  1:357721/141‑1 (MQ=255)

                                          aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAAtt                                                                                              <  2:641236/141‑1 (MQ=255)

                                            acgttAAGTTTTTTGCGCAATATAGCAAATTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTAc                                                                                            <  1:676280/138‑1 (MQ=255)

                                            aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTAc                                                                                            <  1:749825/141‑1 (MQ=255)

                                                 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTaaa                                                                                       <  2:299414/141‑1 (MQ=255)

                                                   tttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAg                                                                                     <  2:471619/140‑1 (MQ=255)

                                                         tGCGCAATTTTGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGa                                                                               <  1:238781/141‑1 (MQ=255)

                                                         tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGa                                                                               <  1:364018/141‑1 (MQ=255)

                                                          gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAGAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAA‑TATAATGTAAAACAATTACGCAAAAGATATGat                                                                              >  2:765545/1‑140 (MQ=17)

                                                          gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGAAGAT‑TATAATGTAAAACAATTACGCAAAAGATATGat                                                                              >  1:614702/1‑140 (MQ=255)

                                                           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGata                                                                             <  2:364865/141‑3 (MQ=255)

                                                           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAACAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGata                                                                             <  1:279765/141‑3 (MQ=14)

                                                           cgaaaTATATCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATGTATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATTATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACa                                                                             <  2:63852/138‑1 (MQ=18)

                                                            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                            <  1:320128/141‑1 (MQ=255)

                                                            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                            >  1:734403/1‑141 (MQ=255)

                                                             cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAATATCAATTAACTAATGCTGAT‑TATAATGTAAAAAAATTACGCCAAAGATATGAtata                                                                           >  1:79126/1‑141 (MQ=14)

                                                                aataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATCAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGATATAccg                                                                        <  2:382414/140‑2 (MQ=14)

                                                                 ataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACaaa                                                                       >  2:614496/1‑141 (MQ=255)

                                                                   aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGATATAcggacg                                                                     >  2:419636/1‑136 (MQ=14)

                                                                    gCAATTTTAAAGGTTCAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAAATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                    <  1:286491/141‑1 (MQ=255)

                                                                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                    >  1:250268/1‑141 (MQ=255)

                                                                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                   >  1:133188/1‑141 (MQ=255)

                                                                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                   <  1:553466/141‑1 (MQ=255)

                                                                           tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                             >  1:804156/1‑141 (MQ=255)

                                                                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACAAACAAATAAAgcg                                                            >  2:647661/1‑141 (MQ=255)

                                                                             aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                           >  1:624413/1‑141 (MQ=255)

                                                                             aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAgagc                                                           >  2:304155/1‑141 (MQ=255)

                                                                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAACTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                          <  2:624731/141‑1 (MQ=255)

                                                                                  ttAATGAATTATAAAGACGGAGATATATCATATAACCCAGAGGTGCCGAGTTATTCAGCCCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                      <  1:145073/141‑1 (MQ=255)

                                                                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                  >  2:775565/1‑141 (MQ=255)

                                                                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                  >  2:456270/1‑141 (MQ=255)

                                                                                      tGAAATATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATATAGCGCCGAAGCtgt                                                  >  1:598001/1‑141 (MQ=255)

                                                                                        aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                <  1:263393/141‑1 (MQ=255)

                                                                                              aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                          <  2:705033/141‑1 (MQ=255)

                                                                                              aaaGATGGAGACATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATCTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                          <  2:212497/141‑1 (MQ=255)

                                                                                                aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGAGATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGACAgg                                        >  2:588196/1‑141 (MQ=255)

                                                                                                       aatatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                 <  2:324518/140‑1 (MQ=255)

                                                                                                          atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTAATGAT‑TATAATATAAAACAATTACGTAAAAGATAAGACATCCCAACAAATAAAGCGACAAAGACAGTGCAGAAAGGAACAgggcaa                              >  2:659280/1‑138 (MQ=14)

                                                                                                          atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAt                              >  2:379954/1‑141 (MQ=255)

                                                                                                          atatCATATAATCCAGAGGTGCCGAGATATACAGCTCAATTACAATTAACCAATGATGAT‑TATAATGTAAAAAAATTACGTAATAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAt                              >  2:412429/1‑141 (MQ=17)

                                                                                                             tCACATAACCCAGAGGTGCCGAGTTATTCAGCTCAATACCAATTAACCAATGATGAT‑TATAATGTAAAACAATTACCTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                           <  2:503417/141‑1 (MQ=14)

                                                                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                    <  1:403226/141‑1 (MQ=255)

                                                                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                    >  1:387304/1‑141 (MQ=255)

                                                                                                                       cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                 >  2:671213/1‑141 (MQ=255)

                                                                                                                          agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCGGAG‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg              >  1:576411/1‑141 (MQ=255)

                                                                                                                          agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGAGAATTT‑AAAGGTTCATCGGt             >  2:311475/1‑141 (MQ=255)

                                                                                                                            gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt            >  2:17804/1‑141 (MQ=255)

                                                                                                                            gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGAAATTTAAAAGGTTCATCAGtt            >  1:289519/1‑141 (MQ=255)

                                                                                                                                 gAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata       <  1:602715/141‑1 (MQ=255)

                                                                                                                                  ggTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat      <  2:319887/140‑1 (MQ=255)

                                                                                                                                     tATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa   >  2:698854/1‑141 (MQ=255)

                                                                                                                                      aTTCAGCTCAATATCCATTGAATAAT‑ATAATAGATAATGTACAACAATTACGTAAAAGATATGACAGACCAACAAATAAAGATCCGAAGCTGGTGGTGAAAGGTAGAGGGTATTGAAAAGGTTCATCAGTTGGATATaaaa  >  2:807279/1‑141 (MQ=14)

                                                                                                                                      aTTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGACGCTGTTGTTGAAAGTTACAGCGACTCTAAAAGGGTCATCAGTTGGATATgaaa  >  2:337665/1‑141 (MQ=17)

TACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAA  >  USA300TCH1516_ALE/111374‑111648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`