BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,516	C→A	21.0%	Q196K (CAA→AAA)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,516	0	C	A	21.0%	213.7 / 40.6	104	Q196K (CAA→AAA)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (42/40); new base A (10/12); total (52/52)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 108 total aligned reads displayed.

CTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAA  >  USA300TCH1516_ALE/111376‑111656

cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                               >  2:403226/1‑141 (MQ=255)

   aGGAAGTTAAAGAAGACAACATAAAAAAAGAAATCGAAAACTTTAAGTGTTGTGCTCAATATCGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                            <  1:784334/141‑1 (MQ=21)

   aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                            <  2:831036/141‑1 (MQ=255)

    gtaaGTTAAAAAATAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                           <  2:158953/139‑1 (MQ=255)

    ggAAGTTAAAGAACAAAACATAAAAAAAGAAATCGAAATCTTTAAGTTTTTTGCGCAATTTAGCAATTTTAAAGATTTAATGAACTATAAAGATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTAGTCGGCTAAata                                                                                                                                           <  1:41754/141‑1 (MQ=18)

       aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTCATTTTTGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGTTTCCGAGTTATTCAGCTCAatataa                                                                                                                                        >  2:263393/1‑139 (MQ=18)

       aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                        >  1:180143/1‑141 (MQ=255)

           aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                                    >  2:66775/1‑141 (MQ=255)

           aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTa                                                                                                                                    >  2:773588/1‑141 (MQ=255)

           aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGGTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGGATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTGACCTCAATATCAATTa                                                                                                                                    >  2:773538/1‑141 (MQ=255)

            aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTACTGAATTATAAAGATGGAGATATATCATATAATCCAGCGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                                   >  1:195211/1‑141 (MQ=255)

            aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                                   <  1:776001/141‑1 (MQ=255)

            aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                                   <  2:472306/141‑1 (MQ=255)

            aagaaaAAAACAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                                   <  2:451054/141‑1 (MQ=255)

             agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                                  >  1:738810/1‑141 (MQ=255)

              gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                                 >  1:247335/1‑141 (MQ=255)

              gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                                 >  1:100592/1‑141 (MQ=255)

              aaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTCGGCTAAATATCAATTAACt                                                                                                                                 <  1:660996/140‑1 (MQ=255)

                agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGATTTATTCGGCTAAATATCAATTAACTaa                                                                                                                               <  2:180143/141‑1 (MQ=255)

                 gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCGGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                              >  1:239577/1‑141 (MQ=255)

                    aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTCGGATAAATATCAATTAACTAatgat                                                                                                                           >  1:400192/1‑141 (MQ=255)

                    aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                           <  1:95953/141‑1 (MQ=255)

                    aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                           <  1:100811/141‑1 (MQ=255)

                     aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATGTAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                          >  2:570541/1‑141 (MQ=255)

                     aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                          >  1:526914/1‑141 (MQ=255)

                     aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                          >  1:721437/1‑141 (MQ=255)

                        tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAATTAACTAATGATGAt‑t                                                                                                                      >  2:756717/1‑141 (MQ=255)

                         aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑ta                                                                                                                     >  2:401272/1‑141 (MQ=255)

                          aacaaaCAAATCGAAAACTTTAAGTTTCTTGCTCAAAATCGCAACTTATAAGATTTAATGAATTATAAAGATGGCGATATATCATATAATTCAGAGGTGCCGAGCTATTCGGCTAAATATCAATTAACTAATGATGAT‑tat                                                                                                                    <  2:122781/138‑1 (MQ=14)

                          aaaaaaGAAATCGAAAACTTAAAGTTTTTTGCGCAATATAGCAACTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATGTGATCAAGAGGTGCCGAGTTTATCAGCTCAATATCAATTAACTAATGATGAT‑tat                                                                                                                    >  2:460188/1‑141 (MQ=21)

                            aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATaa                                                                                                                  <  2:468740/141‑1 (MQ=255)

                                gAAATCTAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTa                                                                                                              <  2:783717/141‑1 (MQ=255)

                                gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTa                                                                                                              >  1:519246/1‑141 (MQ=255)

                                 aaaTCGTAATCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaa                                                                                                             <  1:680882/141‑1 (MQ=255)

                                 aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTaa                                                                                                             <  2:667374/141‑1 (MQ=255)

                                   aTCGAATACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaaaa                                                                                                           <  2:818689/141‑1 (MQ=255)

                                   aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTaaaa                                                                                                           <  1:124134/141‑1 (MQ=255)

                                    ttgAAAAGTTTAAGTTTTTGGCGCGATATGGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATATTCCAGAGGTGCCGAGTTATTCATCTCAATATCAATTAACTAATGATGAT‑TATAATGTAacac                                                                                                          <  2:423474/139‑1 (MQ=18)

                                    tCGGAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                          <  2:657947/141‑1 (MQ=255)

                                    tCGAAAACTTTAAGTTTTTTTTTTTTTTTAGAAATTATAAAGGTTTAATGAATTATAAAGATGGAGTTGTGTCATATAATCTATAGTTGCCGAGTTATTAAGCTCAATATCATTTAAATATTGTTGAT‑TTTATTGTataat                                                                                                          >  2:221110/1‑140 (MQ=255)

                                    tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                          <  2:118504/141‑1 (MQ=255)

                                    tCGAAAACTTTAAGTTTTTAGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                          >  1:502009/1‑141 (MQ=255)

                                    tCGAAAACTTTAAGTCTTTCGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAAc                                                                                                          >  1:624731/1‑141 (MQ=255)

                                       aaaaCTTTAAGTTTTTTGCGGAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAAt                                                                                                       <  2:647745/141‑1 (MQ=255)

                                       aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAAt                                                                                                       <  1:357721/141‑1 (MQ=255)

                                        aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAAtt                                                                                                      <  2:641236/141‑1 (MQ=255)

                                          acgttAAGTTTTTTGCGCAATATAGCAAATTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTAc                                                                                                    <  1:676280/138‑1 (MQ=255)

                                          aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTAc                                                                                                    <  1:749825/141‑1 (MQ=255)

                                               aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTaaa                                                                                               <  2:299414/141‑1 (MQ=255)

                                                 tttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAg                                                                                             <  2:471619/140‑1 (MQ=255)

                                                       tGCGCAATTTTGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGa                                                                                       <  1:238781/141‑1 (MQ=255)

                                                       tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGa                                                                                       <  1:364018/141‑1 (MQ=255)

                                                        gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAGAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAA‑TATAATGTAAAACAATTACGCAAAAGATATGat                                                                                      >  2:765545/1‑140 (MQ=17)

                                                        gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGAAGAT‑TATAATGTAAAACAATTACGCAAAAGATATGat                                                                                      >  1:614702/1‑140 (MQ=255)

                                                         cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGata                                                                                     <  2:364865/141‑3 (MQ=255)

                                                         cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAACAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGata                                                                                     <  1:279765/141‑3 (MQ=14)

                                                         cgaaaTATATCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATGTATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATTATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                     <  2:63852/138‑1 (MQ=18)

                                                          gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                    <  1:320128/141‑1 (MQ=255)

                                                           cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAATATCAATTAACTAATGCTGAT‑TATAATGTAAAAAAATTACGCCAAAGATATGAtata                                                                                   >  1:79126/1‑141 (MQ=14)

                                                               ataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACaaa                                                                               >  2:614496/1‑141 (MQ=255)

                                                                 aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGCAAAAGATATGATATAcggacg                                                                             >  2:419636/1‑136 (MQ=14)

                                                                  gCAATTTTAAAGGTTCAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAAATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                            <  1:286491/141‑1 (MQ=255)

                                                                  gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                            >  1:250268/1‑141 (MQ=255)

                                                                   cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                           >  1:133188/1‑141 (MQ=255)

                                                                   cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                           <  1:553466/141‑1 (MQ=255)

                                                                         tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                     >  1:804156/1‑141 (MQ=255)

                                                                          aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACAAACAAATAAAgcg                                                                    >  2:647661/1‑141 (MQ=255)

                                                                           aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                   >  1:624413/1‑141 (MQ=255)

                                                                           aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAgagc                                                                   >  2:304155/1‑141 (MQ=255)

                                                                            aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAACTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                  <  2:624731/141‑1 (MQ=255)

                                                                                ttAATGAATTATAAAGACGGAGATATATCATATAACCCAGAGGTGCCGAGTTATTCAGCCCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                              <  1:145073/141‑1 (MQ=255)

                                                                                    tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                          >  2:456270/1‑141 (MQ=255)

                                                                                    tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                          >  2:775565/1‑141 (MQ=255)

                                                                                    tGAAATATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATATAGCGCCGAAGCtgt                                                          >  1:598001/1‑141 (MQ=255)

                                                                                      aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                        <  1:263393/141‑1 (MQ=255)

                                                                                            aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAATGTCATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                                  <  1:575769/141‑1 (MQ=255)

                                                                                            aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                                  <  2:705033/141‑1 (MQ=255)

                                                                                            aaaGATGGAGACATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATCTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                                  <  2:212497/141‑1 (MQ=255)

                                                                                              aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGAGATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGACAgg                                                >  2:588196/1‑141 (MQ=255)

                                                                                                     aatatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                         <  2:324518/140‑1 (MQ=255)

                                                                                                       tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                       >  2:319548/1‑141 (MQ=255)

                                                                                                        atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTAATGAT‑TATAATATAAAACAATTACGTAAAAGATAAGACATCCCAACAAATAAAGCGACAAAGACAGTGCAGAAAGGAACAgggcaa                                      >  2:659280/1‑138 (MQ=14)

                                                                                                        atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAt                                      >  2:379954/1‑141 (MQ=255)

                                                                                                        atatCATATAATCCAGAGGTGCCGAGATATACAGCTCAATTACAATTAACCAATGATGAT‑TATAATGTAAAAAAATTACGTAATAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAt                                      >  2:412429/1‑141 (MQ=17)

                                                                                                           tCACATAACCCAGAGGTGCCGAGTTATTCAGCTCAATACCAATTAACCAATGATGAT‑TATAATGTAAAACAATTACCTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                   <  2:503417/141‑1 (MQ=14)

                                                                                                                  aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                            <  1:403226/141‑1 (MQ=255)

                                                                                                                  aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                            >  1:387304/1‑141 (MQ=255)

                                                                                                                     cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                         >  2:671213/1‑141 (MQ=255)

                                                                                                                        agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCGGAG‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg                      >  1:576411/1‑141 (MQ=255)

                                                                                                                        agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGAGAATTT‑AAAGGTTCATCGGt                     >  2:311475/1‑141 (MQ=255)

                                                                                                                          gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                    >  2:17804/1‑141 (MQ=255)

                                                                                                                          gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGAAATTTAAAAGGTTCATCAGtt                    >  1:289519/1‑141 (MQ=255)

                                                                                                                               gAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata               <  1:602715/141‑1 (MQ=255)

                                                                                                                                ggTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat              <  2:319887/140‑1 (MQ=255)

                                                                                                                                    aTTCAGCTCAATATCCATTGAATAAT‑ATAATAGATAATGTACAACAATTACGTAAAAGATATGACAGACCAACAAATAAAGATCCGAAGCTGGTGGTGAAAGGTAGAGGGTATTGAAAAGGTTCATCAGTTGGATATaaaa          >  2:807279/1‑141 (MQ=14)

                                                                                                                                    aTTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGACGCTGTTGTTGAAAGTTACAGCGACTCTAAAAGGGTCATCAGTTGGATATgaaa          >  2:337665/1‑141 (MQ=17)

                                                                                                                                         gCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaact     >  2:836845/1‑139 (MQ=255)

                                                                                                                                          cTCAATATCAATAAACTAATGATGAT‑TATAAAGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGAAAATAAAAAAATTg    <  2:254370/141‑1 (MQ=255)

                                                                                                                                            cAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGGTATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAGTGaa  <  1:570541/141‑1 (MQ=255)

                                                                                                                                            cAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGaa  <  2:721437/141‑1 (MQ=255)

CTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAT‑TATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAA  >  USA300TCH1516_ALE/111376‑111656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`