| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,455,698 | 2 bp→AT | 100% | coding (1216‑1217/1260 nt) | lyrA → | Lysostaphin resistance protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,455,698 | 0 | C | A | 78.7% | 189.9 / 53.2 | 75 | coding (1216/1260 nt) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base A (29/29); minor base . (13/3); total (43/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | USA300TCH1516_ALE | 2,455,699 | 0 | A | T | 98.2% | 217.1 / ‑3.4 | 77 | H406L (CAT→CTT) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (40/34); minor base G (2/0); total (43/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTTGAG > USA300TCH1516_ALE/2455566‑2455839 || tCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑aaa < 1:761292/141‑1 (MQ=255) aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAACAAAAC‑TATCG‑aaaaa > 1:714826/1‑141 (MQ=255) aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGACGACAGACGTTCATCAGTTGAAATT‑CTGGCGAATAAATTATCG‑AAAAAACTg < 2:593979/141‑1 (MQ=255) aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTg > 1:807011/1‑141 (MQ=255) aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAAACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTg > 2:70219/1‑141 (MQ=255) aaCAAGCACCACAAAGCGTCGTTGTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGATTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTga < 1:491548/141‑1 (MQ=255) cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTgaag > 2:832887/1‑141 (MQ=255) aaGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAACAGAGCAAGCATTAAAAGAACCTGCGACTTATAGAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTgaaga < 1:618632/141‑1 (MQ=255) aGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTgaagaa > 1:205754/1‑141 (MQ=255) acaccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCACTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATTCA‑AAAAAATTATCG‑AAAAAACTGAAGAAc < 2:94948/140‑1 (MQ=255) aaaaaGTTACGAATTACCTAACGAATAAAAATAAGATGAAATAGAGCAATTATTAAAAGAAATTGCGACTTAAAAAGAATACAGACGTTCATCAGTTGTAAAAAAAAAA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCt < 2:653521/139‑1 (MQ=255) aaaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTc < 1:656697/141‑1 (MQ=255) atcgACGATTCAACAAACAAAACAAACAAAGATGAAAGAGCGCAATCATTAAAAGAACCTACGACTTAAAAAAAATACAGACGTTCATCAGTTGAAAAAAAAAAA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAg < 2:767694/138‑1 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATaaaa > 2:833793/1‑141 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATaaaa > 2:601247/1‑141 (MQ=255) aTTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGCAGAATGGCATCTTTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATaaaaa > 1:823350/1‑141 (MQ=255) aTTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATaaaaa > 1:182089/1‑141 (MQ=255) aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACgaaa > 2:589076/1‑140 (MQ=255) aaCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAaataat < 1:674119/141‑1 (MQ=255) gATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAA‑TTATCGAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc < 1:810622/141‑1 (MQ=255) aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCACAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc > 2:648872/1‑141 (MQ=255) aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCAAgcg > 1:82026/1‑141 (MQ=255) aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg < 1:110022/141‑1 (MQ=255) aTGAAGATGAAATAGAGCAATCATTACAAGAACCTGCGACTTATAAAGAAGACAGACGATCATCAGTTGTAATTGATGCA‑AGAAAATTATAG‑AAAAAACTGAGGAAAAAACTTCAGATAAAAACCAAAAATAACAAAgccc > 2:295378/1‑139 (MQ=255) aaGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAACCGCTTg < 2:462410/141‑1 (MQ=255) aGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCATATGAAAACAAATAATAACAAAGCGCTTgg > 1:56223/1‑140 (MQ=255) aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCGGATAAAAACAAATAATAACAAAGCGCTTGCTa < 2:205754/141‑1 (MQ=255) aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTa < 2:140085/141‑1 (MQ=255) tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGGTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGCAGAACAATCTTCAGATAAAAACAAATAATAACAAAGAGCATGCTAg > 2:434183/1‑141 (MQ=255) tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATAAGTTGTAATTGATGCA‑AAAAAAGTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAACGCATGCTaa > 2:486811/1‑140 (MQ=255) aTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGAAGCTCATCAGTTGTAATGGATGCAAAAAAA‑GGATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTGGTAcc > 1:166105/1‑141 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct > 2:526276/1‑141 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct < 1:597198/141‑1 (MQ=255) agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt < 1:234514/141‑1 (MQ=255) gCAATCATTAAAAGAACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAATGCGCCTGCTAGTACCTCGTa > 1:312989/1‑141 (MQ=255) gCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTa > 2:58024/1‑141 (MQ=255) aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa < 1:144533/141‑1 (MQ=255) aTCAGTAAAAGGACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATACA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa < 2:602030/141‑1 (MQ=255) tCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAATAACAGGCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa > 1:821815/1‑141 (MQ=255) aTTAAAAGAACCTGCGACTTATAAAGACGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCAAGCGCTTGCTGGTACCACATAAAAAga > 2:450013/1‑141 (MQ=255) ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACCAATAATAACAAAGCGATTGCTAGTACATCTTAAAAAgag > 2:499758/1‑140 (MQ=255) ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTCCCTCTTAAAAAgat > 2:271792/1‑141 (MQ=255) ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat > 1:643733/1‑141 (MQ=255) ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGGTGTAATCGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCCTCAGATAAAAACAAATAATACCAAAGCGCTCGCTAGTACCTCTTaacaaaaa > 2:187055/1‑138 (MQ=255) gAACCGGCAGCTTATAAATAAGACAGACGTTCATCAGGTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt < 2:696823/141‑1 (MQ=255) aCCTGCGACTTATAAAGAAGACATACGTTCAGCAGTTGTAATTGATGCACAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATTCTAg < 2:508202/141‑1 (MQ=255) cTGCGACTTGTAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 2:135175/141‑1 (MQ=255) cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 1:601247/141‑1 (MQ=255) cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 1:833793/141‑1 (MQ=255) tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa < 1:70219/141‑1 (MQ=255) tGCGACTCATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGTA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAACaa > 2:589469/1‑141 (MQ=255) tGCGAATTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCTTGCTCAAGTCCGTTATTAAGATGATGCTAGCaa < 2:385452/141‑1 (MQ=255) gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTATAAAGATGATGCTAGCAAg > 1:777329/1‑141 (MQ=255) cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc < 1:681894/141‑1 (MQ=255) cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc > 2:799925/1‑141 (MQ=255) aCTTATAGAGAACACAGACGTTCATCAGTTCTCATTGA‑GTTAATTAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTATCTTTTAAATAGATGATGCTAGCACgcgc < 2:174058/141‑1 (MQ=255) aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc > 1:217117/1‑141 (MQ=255) ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCCTGCTAGTACCTCTTAAAAGGATGATGCTAGCAAGCGAtttt > 2:662821/1‑141 (MQ=255) taAAGAAGACAGACGTCCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc > 1:498766/1‑141 (MQ=255) aaaGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTct < 2:802917/141‑1 (MQ=255) agtacagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac < 2:474622/138‑1 (MQ=255) aagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAATACTGAAGGACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCTAGCGCTTTTctatac < 1:618272/141‑1 (MQ=255) acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat > 2:157095/1‑141 (MQ=255) agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATCCtatat > 1:181988/1‑141 (MQ=255) agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat > 1:268343/1‑141 (MQ=255) acGTTCATCAGTTGTGATTGATGCACAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTatatat > 1:421706/1‑141 (MQ=255) acGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAGACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTCAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatatct > 2:232502/1‑139 (MQ=255) cGTTCATCAGTTGTAATTGCTGCATAACAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGAAAGCGCTTTTCTATACtatatagt > 2:180447/1‑139 (MQ=255) cGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt > 2:250005/1‑141 (MQ=255) catcaGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttt > 1:324568/1‑141 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTCttttt > 1:139023/1‑141 (MQ=255) aGTTGTAATTGATGCAAAAAAA‑TTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTCGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCt > 1:526299/1‑141 (MQ=255) aaTTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt < 1:387033/141‑1 (MQ=255) aTTGATGCA‑AAAAAATTATCG‑AACAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt < 1:635854/141‑1 (MQ=255) aTTGATGCA‑AAAAAATTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAGGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt > 1:123535/1‑141 (MQ=255) caaaaaaaTTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACAcc < 1:714541/139‑1 (MQ=255) aaaaaaTTATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCct < 1:46826/141‑1 (MQ=255) ataaCG‑AAAAAACTGAAGAACAATCTGCAAATAAAAACAAAGAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTTGAg < 1:342897/141‑1 (MQ=255) || TCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCG‑AAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTTGAG > USA300TCH1516_ALE/2455566‑2455839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |