Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5120AG33.3% 187.0 / 145.3 165S194S (TCA→TCG) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (59/51);  new base G (21/34);  total (80/85)
Fisher's exact test for biased strand distribution p-value = 7.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 167 total aligned reads displayed.

AATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTA  >  USA300TCH1516_ALE/111364‑111660
                                                                                                                                                    |                                                                                                                                                     
aaTTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCa                                                                                                                                                       <  1:51352/149‑1 (MQ=255)
 aTTCATTTCTACTGAGGAAGTTAAATAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                                      <  1:338461/149‑1 (MQ=255)
 aTTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                                      <  1:183552/149‑1 (MQ=255)
  ttCATTTCTACTGAGGAAGTTCAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                                                                     <  1:604757/149‑1 (MQ=255)
  ttCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAAGTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGc                                                                                                                                                     <  2:615020/149‑1 (MQ=255)
  ttCATTTCAACTGAGGAAGTTAAAGAAGAAAACAGAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGc                                                                                                                                                     <  1:1074198/149‑1 (MQ=255)
     aTTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCtaa                                                                                                                                                  <  1:868079/149‑3 (MQ=255)
      tttCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCaa                                                                                                                                                 <  1:1071636/149‑1 (MQ=255)
          tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATc                                                                                                                                             <  1:449676/149‑1 (MQ=255)
          tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATc                                                                                                                                             <  2:636504/149‑1 (MQ=255)
            cggagaAAGTTAAAGAAGAAAACATAAAAAAATAAATCGAAAACTTTAAGTTGTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCaa                                                                                                                                           <  2:314564/147‑1 (MQ=255)
               aGGAAGTTAAAGAAGAAAACATAAAAAAGGAAATCGAAAACTTTAAGTTTTTTGCGCAATGTAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGCCTAGTTATTCGGCTAAATATCAATTa                                                                                                                                        <  1:576629/149‑1 (MQ=18)
               aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTAGCAATTAAAAAGATTTAATGAATTATAAATATGGAGATATATCATATAATCCAGCGGTGCCGACTTATTCAGCTCAATAACAAATa                                                                                                                                        >  2:1109295/1‑149 (MQ=18)
               aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                                        <  1:423559/149‑1 (MQ=255)
               aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTa                                                                                                                                        >  1:925364/1‑149 (MQ=255)
                ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                                       >  2:413291/1‑149 (MQ=255)
                 ggaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                                      <  2:27371/147‑1 (MQ=255)
                 gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                                      <  2:896214/149‑1 (MQ=255)
                 gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                                      <  1:949984/149‑1 (MQ=255)
                  aaGTTAAAGAAGAAAACATAAAAAAAGCCAAACAAAACTGTAAGTTTTTTGCGCAATATCGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGGTGCAGAGATATTCAGCTCAATATCAATTAACt                                                                                                                                     >  2:792848/1‑149 (MQ=255)
                     ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGTAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                                  >  1:369597/1‑149 (MQ=255)
                      tAAAGAAGAAAACAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatg                                                                                                                                 <  2:140615/149‑1 (MQ=255)
                        attaataaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGCTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                               <  2:829150/143‑1 (MQ=18)
                         agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTGTAATATAGCAATATTAAAGAGTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTGAGCTCAATATCAATTAACTAatgatg                                                                                                                              >  2:343130/1‑149 (MQ=18)
                         agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTTAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGGCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                              <  1:720847/149‑1 (MQ=255)
                         agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                                              >  2:1022655/1‑149 (MQ=255)
                         agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                                              >  2:66203/1‑149 (MQ=255)
                         agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                                              >  2:619284/1‑149 (MQ=255)
                          gaataaAACAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                             <  2:945357/149‑1 (MQ=255)
                          gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTTCAATTTAAAAGATTAAATGAATTCTAAAGTTGTGGATAGATTATATAATCCAGAGGTTCCGAGTTATTAAGCTAAATATAAACTAACTAttgataa                                                                                                                             >  2:208336/1‑147 (MQ=12)
                          gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                             <  1:1109613/149‑1 (MQ=255)
                          aaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                             <  2:110692/148‑1 (MQ=255)
                           aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                                            >  2:449682/1‑149 (MQ=255)
                           aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgacgct                                                                                                                            >  2:259939/1‑145 (MQ=255)
                           aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATgatgat                                                                                                                            <  1:638963/149‑1 (MQ=255)
                           aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATgaagat                                                                                                                            >  2:895832/1‑149 (MQ=255)
                            agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                           >  1:442772/1‑149 (MQ=255)
                            agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                           >  1:1018798/1‑149 (MQ=255)
                              aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                         <  1:190049/149‑1 (MQ=255)
                              aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                         >  1:780303/1‑149 (MQ=255)
                                     aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                                  >  2:678804/1‑149 (MQ=255)
                                      aaaaaaGAAATCGAAAACTTTAAGTTTTTTTTGTTTTTTTGCAAGGAAAAAGAGTTAATGAATTATAAAGTTGTAGATATATCATATAATCCAGAGGTGCCGAGTTATACAGAAAAATATCAATTAACTAATGATGATTATAATTTata                                                                                                                 >  2:653470/1‑147 (MQ=12)
                                       aaaaaGAAATCCAAAACCGTAAGTTTTTTGCGCAAGACAGTAATTTTAAATATTTAATCAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                                <  2:426245/149‑1 (MQ=18)
                                         aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGGTAAATATCAATTAACTAATGATGATTATAATGTAAGACa                                                                                                              >  1:287725/1‑149 (MQ=255)
                                         aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                                              >  1:404885/1‑149 (MQ=255)
                                          aagaaagaGAACACTTTTAGTTTTGTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACac                                                                                                             <  2:848489/149‑2 (MQ=18)
                                             aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTGATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTa                                                                                                          <  1:1049267/149‑1 (MQ=255)
                                               aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                                        <  2:728988/149‑1 (MQ=255)
                                                   aataCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaa                                                                                                    <  2:864777/146‑1 (MQ=255)
                                                     aaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaaa                                                                                                  >  2:458899/1‑148 (MQ=255)
                                                     aaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAg                                                                                                  <  2:54216/149‑1 (MQ=255)
                                                     aaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATAATTATAATGTAAAACAAGCACGTAAAAg                                                                                                  >  1:274063/1‑149 (MQ=255)
                                                        tttAATTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGata                                                                                               <  2:360894/149‑1 (MQ=255)
                                                        tttAAGTTTGTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGata                                                                                               <  1:610817/149‑1 (MQ=17)
                                                         ttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGatat                                                                                              <  2:101841/149‑1 (MQ=255)
                                                           aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGa                                                                                            <  2:753639/149‑1 (MQ=255)
                                                           aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGCCCAAACATCACAAAATCAAGAGCTGTAGCCTTTTTTACATACATATTAATTAACTAAGTAGTATTTTTTGGTAAAACAAGTACTTATAAGATTTGa                                                                                            >  2:927450/1‑149 (MQ=255)
                                                           aaGTTTTTTGCCAAATATAGCAATTTTAAAAATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGCCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                                            <  2:559785/149‑1 (MQ=14)
                                                            aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGat                                                                                           <  1:156524/149‑2 (MQ=255)
                                                              ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                         >  1:55615/1‑149 (MQ=255)
                                                                ttttGCGCAATAGAGCAATTTTAAAGATTTAATGAATTATAAAGATGGTGATATATGATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                                                                       <  1:821897/149‑1 (MQ=14)
                                                                  ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccg                                                                                     >  1:102497/1‑148 (MQ=17)
                                                                   tGCGCAATATAGCAATTATAAAGAGGTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                                    <  1:253132/149‑3 (MQ=11)
                                                                     cgcgATATAGAAATGTTAAAGATTTAATGAATTATACAGATGGAGATATATCATATAATCTAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                                                                  <  2:550986/149‑2 (MQ=11)
                                                                     cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATTTCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacg                                                                                  <  2:527536/149‑5 (MQ=14)
                                                                      gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                                                                 <  2:885883/149‑3 (MQ=11)
                                                                      gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                 <  2:580330/149‑1 (MQ=255)
                                                                       cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgag                                                                                <  2:859603/149‑7 (MQ=11)
                                                                            taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                                                                           >  1:221912/1‑149 (MQ=11)
                                                                             aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGGCATACCAACAAATAAAgc                                                                          >  1:421615/1‑149 (MQ=255)
                                                                             aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                          >  2:418608/1‑149 (MQ=255)
                                                                             aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGTAAAAGATATGACATACCAACAAATAAAgc                                                                          >  2:946758/1‑149 (MQ=255)
                                                                               cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTTAAAAGATATGACATACCAAAAAATAAAgcgc                                                                        >  2:170393/1‑149 (MQ=255)
                                                                                  tcttAAACATTTAATGAATTATAAAGATGAAGATATAGCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                     <  1:370452/147‑1 (MQ=255)
                                                                                       aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                >  1:162969/1‑149 (MQ=255)
                                                                                        aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAACCAATTACGTAAAAGATATGACATACCAACAAATAAAGAGCCGAAGCtgt                                                               >  2:657903/1‑149 (MQ=255)
                                                                                          aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAACGCGCCGAAGCtgttg                                                             <  1:1105459/149‑1 (MQ=255)
                                                                                              aaTGAATCATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAGAATTAGGTAAAAGATATCACATACCAACAAATAAAGTGATGCAGGTgttgttta                                                         >  2:914870/1‑147 (MQ=17)
                                                                                                 taaTTATAAAAATGGAGATAGATCATAGAATCCAGTGGTGCCGAGTGGTTCAGTGCAAGATCAATTAATTAATTATGATTATAATGTAAACCAATTACGGCAAGTATATGACATACCGACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAg                                                     <  2:378329/148‑1 (MQ=255)
                                                                                                 gAATTATAAAGATGGAGATATATCATACAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAg                                                     <  1:454413/149‑1 (MQ=255)
                                                                                                    ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTa                                                  <  2:499375/149‑1 (MQ=255)
                                                                                                      ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACa                                                <  1:59641/149‑1 (MQ=255)
                                                                                                          aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGa                                            <  1:449514/149‑1 (MQ=255)
                                                                                                          aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGa                                            <  1:695153/149‑1 (MQ=255)
                                                                                                            aTGGAGATATAAAATATAATAGAGAGGTGCCGAGTTATTTAGTTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAACTAAAGAGCCGAAGCTG‑TGTTGCTAAGGTACAGGCAAt                                          >  2:380026/1‑149 (MQ=17)
                                                                                                             tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACCGGGAAtt                                         >  2:129212/1‑149 (MQ=255)
                                                                                                              ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGGAAAACAATTACGAAAAAGATATGAAATACCAAAAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGCAAatt                                        >  2:979078/1‑149 (MQ=17)
                                                                                                                    atatCATATAATCCAGAGGTGCCGAGTGATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAGGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAgg                                  <  1:21340/149‑1 (MQ=255)
                                                                                                                     tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAGCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATCTAAAAGGt                                 >  1:835435/1‑149 (MQ=255)
                                                                                                                      atCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAATACGTAAAAGATATAAGATACCAAAAAATAAAGCGCCGAAGCTGTAGTTG‑AAAGGTAAAGGAAATTTAAAAGGtt                                >  2:333129/1‑149 (MQ=17)
                                                                                                                        cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTCCGTAAAAGATATGACATACAAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTtca                              >  2:336400/1‑149 (MQ=255)
                                                                                                                        cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAGTTTAAAAGGTtca                              >  1:109233/1‑149 (MQ=255)
                                                                                                                        cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAAAGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTtca                              >  2:1094071/1‑149 (MQ=255)
                                                                                                                             aaTCCAGAGGTGCCGAGTTATTCAGCTCAGTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCAGt                         >  1:574683/1‑149 (MQ=255)
                                                                                                                              aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAGTTAACTAATGGTGATTAGAATGTGAAACAATTAGGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAAGTTAAAAGGTTCATCAGtt                        >  2:819135/1‑149 (MQ=14)
                                                                                                                               tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGAATCAACAGTTg                       >  2:505793/1‑149 (MQ=255)
                                                                                                                                      gTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTTTGTTCTATACCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa                <  1:916662/149‑1 (MQ=14)
                                                                                                                                          cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCCGATGGATATaaaaaa            >  1:520381/1‑149 (MQ=255)
                                                                                                                                          cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa            >  2:481369/1‑149 (MQ=255)
                                                                                                                                            aattATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt          <  2:576965/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTG‑AAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTA  >  USA300TCH1516_ALE/111364‑111660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑