Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,416,458 A→T I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,416,4580AT98.4% 222.3 / NA 65I216N (ATT→AAT) oppD_4Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (30/33);  minor base G (0/1);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTG  >  USA300TCH1516_ALE/1416315‑1416599
                                                                                                                                               |                                                                                                                                              
cccaTGTTTTAACTCAATCAGAGTAATCCCTCTTTAATTGTGTTCTATGTTTATTTAGACGGTCCGTACACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAATACATAATTGTAc                                                                                                                                           <  1:760833/147‑1 (MQ=255)
cacaTGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAc                                                                                                                                           >  1:739152/1‑149 (MQ=255)
     gTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTCATTAGACGTTCAGGATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                                                                      <  1:367761/149‑1 (MQ=255)
      ttttAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACaa                                                                                                                                     >  1:285983/1‑149 (MQ=255)
           aCTCAACCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCACCAGCACAGGCTTGACAAGAAAGAGATGACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑Attt                                                                                                                               <  1:1096531/149‑1 (MQ=255)
                    tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATg                                                                                                                      <  1:451638/149‑1 (MQ=255)
                    tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATg                                                                                                                      <  2:760605/149‑1 (MQ=255)
                      gtgaTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATCATTGTACTGACAAT‑ATTTCACTACATgcc                                                                                                                    >  2:135228/4‑147 (MQ=255)
                       tAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACt                                                                                                                   >  1:428779/1‑149 (MQ=255)
                         aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACtaa                                                                                                                 >  2:1096507/1‑147 (MQ=255)
                          tCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTaa                                                                                                                <  2:1113368/149‑1 (MQ=255)
                            ccTCTGTAATTGTGTTCTATATTTAAGTAGACGTTCAGTATACGGATGCAAATGTTCATACTTGAAATGATTAATATTACCTCGTTTAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGt                                                                                                              <  2:731491/149‑1 (MQ=255)
                             ctctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAACATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGtt                                                                                                             >  1:964148/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAt                                                                                                           >  2:636099/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAt                                                                                                           >  1:897620/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAt                                                                                                           >  1:897600/1‑149 (MQ=255)
                               ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGAAACTGCTCAGACTTGAAATGATTAATATTACCTCGGTCAATAATTTGACCTTTTTTTAAAACATAATTGTACTG‑CAATAATTTCAATACATTAATTAAGTTAt                                                                                                           >  2:724354/1‑149 (MQ=255)
                                tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAtg                                                                                                          <  2:580973/149‑1 (MQ=255)
                                tttAATTGTGTTCTATATTTAATTAGACGTTCAATATACGGATGCAAATGCTCATGCTTGAAATGATTAATATTCCCTCGTTCAATGATTTGACCTACTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAtg                                                                                                          <  2:614670/149‑1 (MQ=255)
                                 ttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAtgt                                                                                                         >  1:29310/1‑149 (MQ=255)
                                  tAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAtgtg                                                                                                        <  1:639832/149‑1 (MQ=255)
                                   aaTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATCTGACCTTCTTTTAAAACATAATTGTACTGACAAC‑ATTTCAATACATGACTTAAGTTAtgtgt                                                                                                       <  1:724267/149‑1 (MQ=255)
                                   aaTTGTGTTCTAGATGTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTAtgtgt                                                                                                       >  2:964522/1‑149 (MQ=255)
                                        tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                                  <  2:156816/149‑1 (MQ=255)
                                           tCTATATTTAATTAGACGTTCAGTATACTGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                               <  2:820967/149‑1 (MQ=255)
                                           tCTATATTTAATTAGACGTTCAGTATACGTATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                               <  1:699012/149‑1 (MQ=255)
                                           tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                               <  2:688182/149‑1 (MQ=255)
                                             tataTTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGCCAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATaatacat                                                                                             >  1:93288/1‑149 (MQ=255)
                                                atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTTTACAGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAaataat                                                                                          >  1:486458/1‑149 (MQ=255)
                                                      ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                                    >  1:245250/1‑149 (MQ=255)
                                                      ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                                    >  1:190725/1‑149 (MQ=255)
                                                       tAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATAATACCTCGTTAAATGATTTGACATTCTTTTAAAAAGTAACTGTGCATGCAAT‑ATTGCAATACATGAAATACGAAATGTGTGATGATAaataatactaaaa                                                                                   >  2:585637/1‑142 (MQ=255)
                                                       tAGACGTTCACTATACGGATGCAAATGCTCATACTTGAAATGATTAACCTTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                   <  1:638061/149‑1 (MQ=255)
                                                        aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                     >  1:881808/1‑146 (MQ=255)
                                                        aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                     <  2:881808/146‑1 (MQ=255)
                                                         gACGTTCAGTATACGGCTACAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGGCCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATACTGTTTGACCa                                                                                 >  2:74289/1‑149 (MQ=255)
                                                         gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                                 >  1:922551/1‑149 (MQ=255)
                                                          aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAAGGTTTGACCAt                                                                                >  2:1055478/1‑149 (MQ=255)
                                                             gtgaGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                             <  1:156998/146‑1 (MQ=255)
                                                                aGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTa                                                                          <  1:996542/149‑1 (MQ=255)
                                                                   ataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATa                                                                       <  2:881919/149‑1 (MQ=255)
                                                                            cAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCt                                                              <  2:420444/149‑1 (MQ=255)
                                                                             aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATCCAATATGCTg                                                             >  1:357056/1‑149 (MQ=255)
                                                                              aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                                            <  2:120187/149‑1 (MQ=255)
                                                                              aaTGCTCATACTTGAAATGATTAATATTACCTCCTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCCATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                                            <  2:512943/149‑1 (MQ=255)
                                                                               aTGCTCATACTTGAAATGATTAATCTTACCTCGTTCAATGATTTGACCTTTTTTTAAAACATAATTGTAGTAGCTGT‑ATTTCAATACGTTACTTAATTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGta                                                           <  1:1096507/149‑1 (MQ=255)
                                                                               aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGGTGta                                                           >  1:469706/1‑149 (MQ=255)
                                                                                          tGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGCTCATTTTCTAATACAATATGCTGTAATAAATCCATc                                                <  2:739152/149‑1 (MQ=255)
                                                                                                taTTAATATTATACCGTTCAATGATTGAACCTTCTGTTCAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGa                                          <  2:563722/148‑1 (MQ=255)
                                                                                                    aaTATTACCTTGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTTACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATTTTTTACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAc                                      <  2:837536/149‑1 (MQ=255)
                                                                                                    aaTATTACCTCGGTCAATGATTTGACCTTCTTTTAAAACATACTTGTACGGACAAT‑ATTTCAATACATGACTTACGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATGCAATATGCTGTAATCAATCCATCACTTGATTAc                                      <  2:536233/149‑1 (MQ=255)
                                                                                                        ttcccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAGTAAATCCATCACTTGATTACCGtt                                  <  1:664170/146‑1 (MQ=255)
                                                                                                        ttACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                                  >  1:234250/1‑149 (MQ=255)
                                                                                                              cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                            >  1:899713/1‑149 (MQ=255)
                                                                                                              cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                            >  2:956697/1‑149 (MQ=255)
                                                                                                              cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                            >  1:355038/1‑149 (MQ=255)
                                                                                                                 tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                         <  1:217696/149‑1 (MQ=255)
                                                                                                                       aTTTGACCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTAATTACCGTACAAAGCATCCAatga                   >  2:567205/1‑149 (MQ=255)
                                                                                                                            aCCTTCTTTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATTCaa              >  1:645346/1‑149 (MQ=255)
                                                                                                                               ttcttTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg           >  2:338467/1‑149 (MQ=255)
                                                                                                                                 cttTTAAAACATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGt         >  2:913052/1‑149 (MQ=255)
                                                                                                                                  ttttAAAAAATAAGTTTACTGACAAT‑ATTTCAATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCACCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGtt        <  2:364247/149‑1 (MQ=255)
                                                                                                                                      aaaaCATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCAACCACTGATGCAACTGGTTCGTc    <  2:756205/149‑1 (MQ=255)
                                                                                                                                      aaaaCATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCAACCACTGATGCAACTGGTTCGTc    <  2:756202/149‑1 (MQ=255)
                                                                                                                                        aaCATAATTGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTg  <  2:99020/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAAT‑ATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTG  >  USA300TCH1516_ALE/1416315‑1416599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑