Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	97.3%	240.6 / NA	76	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (20/53);  minor base T (1/1);  total (22/54)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTA  >  USA300TCH1516_ALE/1646044‑1646332                                                                                                                                                  |                                                                                                                                                 ttCAATAATTTTATCACTTGCCACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                               <  2:712392/149‑1 (MQ=255) ttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                               <  2:813031/149‑1 (MQ=255) ttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                               >  1:147240/1‑149 (MQ=255) ttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                               <  2:802696/149‑1 (MQ=255)   cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCATATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTGTTTCAGTCATGCCTCCtttt                                                                                                                                             <  1:582112/149‑1 (MQ=255)   cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTCTtttt                                                                                                                                             >  2:944966/1‑149 (MQ=255)   cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                             <  1:930259/149‑1 (MQ=255)    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                            <  1:719473/149‑1 (MQ=255)    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                            <  1:99150/149‑1 (MQ=255)    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                            <  1:791808/149‑1 (MQ=255)        atTTTCTCACTTTCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                        <  1:1085112/149‑1 (MQ=255)        atTTTATCCCTTGCAACACTATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                        <  2:38977/149‑1 (MQ=255)        atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                        <  2:1049467/149‑1 (MQ=255)        atTTTATCACTTGCAACACCATGTGGTAACGCACCTCTATTACCAGATGCTACAATCGTATCGAATGATCGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                        <  2:991265/149‑1 (MQ=255)        atTTTATCACGTGCCACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                        <  2:717246/149‑1 (MQ=255)           ttattcgttGCACCGCCACGTGGTAACGGACTTCTGTGACAGGAGGCTACAATCGTATAGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                     <  1:425735/142‑1 (MQ=255)           tgaTCACTTGCAACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTACTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                     <  1:674079/147‑1 (MQ=255)            tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                    <  2:99450/149‑1 (MQ=255)               cccTTGCAACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                                 <  2:1029979/147‑1 (MQ=255)               cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                                 >  2:605108/1‑149 (MQ=255)                     cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTTCTTTTACTACAATTAAAatatat                                                                                                                           >  1:664951/1‑149 (MQ=255)                     cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAACTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                           <  1:990000/149‑1 (MQ=255)                      aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                                                                                          >  1:408135/1‑149 (MQ=255)                                 gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtca                                                                                                               >  2:1044744/1‑149 (MQ=255)                                 gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtca                                                                                                               >  2:632445/1‑149 (MQ=255)                                   aaTGCACCGCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATTTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                                                                             <  2:990608/149‑1 (MQ=255)                                    aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                            <  1:229315/149‑1 (MQ=255)                                      gCACCTCTATGACCAGATGCTACCATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaac                                                                                                          <  2:524527/149‑1 (MQ=255)                                           cctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTGTACTACATTTAAAATATATTCATATGTTTCATCAACAatat                                                                                                     <  2:739121/148‑1 (MQ=255)                                            ttATGACCAGATGCTACTATCGTATCGAATGATGCTCTATGCTCTCTGAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAtt                                                                                                    <  1:581498/148‑1 (MQ=255)                                                    aGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTTATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                            <  1:392705/149‑1 (MQ=255)                                                        gCTACTATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTg                                                                                        <  2:376636/149‑1 (MQ=255)                                                        gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTg                                                                                        <  1:742662/149‑1 (MQ=255)                                                         cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGa                                                                                       <  2:1040191/149‑1 (MQ=255)                                                          caCAATCTTACCCAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGaa                                                                                      <  1:395608/148‑1 (MQ=255)                                                          caCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGaa                                                                                      <  1:299763/148‑1 (MQ=255)                                                           aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                                     >  2:173546/1‑149 (MQ=255)                                                              aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                                  <  1:910935/149‑1 (MQ=255)                                                                cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATCTTAGCTGCTTTTTGAATTAAAg                                                                                >  2:939373/1‑149 (MQ=255)                                                                  tATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                                              >  1:122954/1‑149 (MQ=255)                                                                   ttCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCaa                                                                             <  2:701247/148‑1 (MQ=255)                                                                   aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCAGCAACAATATTAGCTGCTTTTTGAATTAAAGCaa                                                                             >  2:622971/1‑149 (MQ=255)                                                                     cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                           <  1:662914/149‑1 (MQ=255)                                                                                   tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑ttt                                                            <  2:612379/149‑1 (MQ=255)                                                                                         ccTAATTCTCGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATACCCATTGGCGGCATTACTAAAGATGGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGtc                                                      <  1:264642/149‑1 (MQ=255)                                                                                           tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTACTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACCATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTAAGAATC‑TTTGACGtctc                                                    >  1:491858/1‑149 (MQ=255)                                                                                             aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTa                                                  <  2:141868/149‑1 (MQ=255)                                                                                               tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAAt                                                >  1:1033972/1‑149 (MQ=255)                                                                                                cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTGTTCAGTCATGCCTTCTTTTACAACAGTTAAAATCTATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAAtt                                               <  1:381694/149‑1 (MQ=255)                                                                                                   gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAAttttt                                            >  1:526754/1‑148 (MQ=255)                                                                                                   gCATTTTGCTTTCTAATATTGCCTTTAATTCCTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTAGTGAG‑ATCTTTTGACGTCTCTCAttttt                                            >  2:451648/1‑148 (MQ=255)                                                                                                    cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTAt                                           <  2:848676/149‑1 (MQ=255)                                                                                                      ttttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCt                                         <  1:286664/149‑1 (MQ=255)                                                                                                        ttGCTTTCTAATATCGCCTTTAATGCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTAc                                       <  2:35696/149‑1 (MQ=255)                                                                                                         tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACa                                      <  2:96097/149‑1 (MQ=255)                                                                                                         tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTAAAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTTAGCAGC‑CTTTACGTCTCTCATGTTATCTACa                                      >  1:85523/1‑149 (MQ=255)                                                                                                           cTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGt                                    <  2:122954/149‑1 (MQ=255)                                                                                                           cTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTACTTTTATCTACAGt                                    >  1:605077/1‑149 (MQ=255)                                                                                                            tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTa                                   <  1:918450/149‑1 (MQ=255)                                                                                                                     tttgcTTTAATTCTTTTTCATGCATGCCTCCTCGTACAACAGTTAAAATATATCCCTATGTTTCATCAACAACATTAGCGGCTTTTTGAATGAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                          <  1:366576/145‑1 (MQ=255)                                                                                                                     ttGCCTCTAATTCCTTTTCAGTACTGCCTCCTTTTACAACAGTGAAAATATATTCATATTTTTCATCAACAATATTATCGGGTGTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                          <  2:989010/149‑1 (MQ=255)                                                                                                                       gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAACTTTATCTACAGTATTAGAAATGCt                        <  1:664912/149‑1 (MQ=255)                                                                                                                       gCCTTTAATTCTTTTCCAGTCCTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt                        <  2:897732/149‑1 (MQ=255)                                                                                                                        ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                       <  1:1046439/149‑1 (MQ=255)                                                                                                                          tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat                     <  1:173546/149‑1 (MQ=255)                                                                                                                           ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACCGTATTAGAAATGCttatt                    >  1:966018/1‑149 (MQ=255)                                                                                                                               ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg                <  2:160882/149‑1 (MQ=255)                                                                                                                               ttCTTTTCCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTTATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg                <  2:133774/149‑1 (MQ=255)                                                                                                                               tgcTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg                <  2:904522/147‑1 (MQ=255)                                                                                                                                tCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa               >  1:660645/1‑149 (MQ=255)                                                                                                                                  tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata             >  2:27762/1‑149 (MQ=255)                                                                                                                                      tCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg         <  2:734749/149‑1 (MQ=255)                                                                                                                                        aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAGTTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc       >  1:374530/1‑149 (MQ=255)                                                                                                                                            aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtttt   >  2:78178/1‑149 (MQ=255)                                                                                                                                             tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTttta  <  1:1044744/149‑1 (MQ=255)                                                                                                                                             tGCCTCCTTTTACAACAGTTAAAATATAGTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTTTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTttta  <  1:632445/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATC‑TTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTA  >  USA300TCH1516_ALE/1646044‑1646332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑