| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,744,473 | C→T | A67T (GCT→ACT) | apt ← | Adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,744,473 | 0 | C | T | 97.8% | 276.7 / NA | 92 | A67T (GCT→ACT) | apt | Adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (35/54); total (36/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAAC > USA300TCH1516_ALE/1744327‑1744616 | tAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTa < 1:686946/149‑1 (MQ=255) aaCACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGCATAAGTTAc > 1:79486/1‑149 (MQ=255) acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCCTACTCATAACGAATGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACa > 2:262455/1‑149 (MQ=255) acacacGTTGACATGGTTTAATTGCATCTTTGTGCATTTTTAAAACATTTATACCATATTCTAGGTCATACTCATAACGAAAGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACa < 2:293095/149‑1 (MQ=255) cacacGTTGCACTGGTTTAATTGCATCTTTGTGCATTGTTAACACATTTGTACCATATTCCAGGTCATACACATAACGAATGACTTCACGAGGCAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAACCCCATTGGATAAGTTACAg > 2:685032/1‑149 (MQ=255) cacGTTGACCTGGTTTAATTGCATCTTCGTGCATTGTTAAAACATCTGTACCACATTCAAGGTCATACTCATAACGAATGACTTCACGAGGTAATCTCCCTTCTTTTCTAACAGGCGCAAAGCCAATCCCCATTGAATAAGTTACAAGa > 2:143842/1‑149 (MQ=255) tttaCCTTGTTTAATTGCATCTTTGTGCATTGTTACTCCCTTTGTATAGTCTTATAAAACGCACACATAACGCATGAATTCACGAGGTTATCTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGc < 2:1067370/146‑1 (MQ=255) ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGc < 2:475751/149‑1 (MQ=255) tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTGCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGa > 2:951725/1‑149 (MQ=255) ggTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGAt < 2:501970/149‑1 (MQ=255) ggTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGAt > 1:156718/1‑149 (MQ=255) ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCTAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCa < 2:723917/149‑1 (MQ=255) tGCATCTTTGTGCATTGTTACAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc < 1:1033027/149‑1 (MQ=255) caccTTTGTGCATTGTTAAAACATTTGTACCATATTCCAGGTCATACTCATAACGAATGACTTAACGAGGTGATTTCCCTTCTTCACTAACAGGTGAAAAGCCAATCCCCCTTGAATAAGTTACACGAGAACCAGTGAAAAAGCCAcgc > 2:356954/4‑149 (MQ=255) cATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGCCTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCACTGAATAAGTTACAGGAAAGCCAATGATAAAGCCCcgc > 2:322314/1‑149 (MQ=255) cATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcgc > 2:945291/1‑149 (MQ=255) cATCTTTGTGCATCGTTAAAACATTTGTACCATGGTCTAGGTCGTACTAATAGCGAATGACTTCAAGAGGTACTTTCCCTTCTTTTCTAACAGGTGCAAAGACAATCCCCATTGGATAAGTTAGAGGACAGCCAATCACAAAGCCCcgc > 2:1087042/1‑149 (MQ=255) ctctgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGACTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCt < 1:365350/146‑1 (MQ=255) gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAATCCACGCGCTTCAGGt < 1:702657/149‑1 (MQ=255) gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt < 1:1014491/149‑1 (MQ=255) gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt < 2:1033451/149‑1 (MQ=255) ttGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAATAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt < 1:719699/149‑1 (MQ=255) tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATGCCCATTGAATAAGTTCCAGAACAGCCAATGATAAAGCCACGCGCTTCAGGcccaa > 2:60672/1‑147 (MQ=255) tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTa < 2:897093/149‑1 (MQ=255) tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTa < 1:231028/149‑1 (MQ=255) ttAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACa < 1:8356/149‑1 (MQ=255) tAAAACATTTGTACCATATTCTAGGTCATACTCATACCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa > 2:10287/1‑149 (MQ=255) tAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCACTCCCCATTGAATAAGATACAGGACAGCCAATGCTAAAGCCACGCCCGTCCAGTCCTACaa > 2:166810/1‑149 (MQ=255) tAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCACTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCATaaaa > 1:962810/1‑149 (MQ=255) aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg < 1:522773/149‑1 (MQ=255) aTTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGatat > 1:786755/1‑149 (MQ=255) tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATc < 2:199087/149‑1 (MQ=255) tACCATATTCTAGGTCATACTCATAACGAATGCCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACa < 2:189908/149‑1 (MQ=255) tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa < 1:313696/149‑1 (MQ=255) atatTCTAGGTCATACTCATAACGAATGACTTTCTGGTGGGATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc > 1:1102007/1‑149 (MQ=255) atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAACGCCAATCCCCATTGAATACGTTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGGTCCTCCCACGTTATCAACAtctt > 1:288611/1‑148 (MQ=255) atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc > 1:735831/1‑149 (MQ=255) atatTCTAGGTCATACTCATAACGAATGACTTCACGAGAAAATTACCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc > 1:436746/1‑149 (MQ=255) ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTgc > 2:523863/1‑148 (MQ=255) ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTCCAGGTCCTACAACGATATCAACATCTCTGt > 2:1084672/1‑149 (MQ=255) ttCTAGGTCATACTCACAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTACCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt < 2:64324/149‑1 (MQ=255) gttaTACTCATATCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACACGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg < 2:64826/146‑1 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACGGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc < 2:117986/149‑1 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATACAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc < 1:871259/149‑1 (MQ=255) cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACTATATCAACATCTCTGTCTTTTGCg < 1:622418/149‑1 (MQ=255) cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAACGAAACAGCCCCGCGCGCCAGGTCCTACACCGATCTCAACCTCTCCGTCATTTGCg > 2:233563/1‑149 (MQ=255) tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑Tc < 1:455365/149‑1 (MQ=255) tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑Tc < 1:455400/149‑1 (MQ=255) cATAACGAATGACTTCACGAGGTCAGTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCt < 1:478781/149‑1 (MQ=255) cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCt < 2:689283/149‑1 (MQ=255) tAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTAc > 1:757630/1‑149 (MQ=255) aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTTTTTTTTTTTCTTAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATATCTGTATTTTTCGTAT‑TCTTCa > 2:82776/1‑149 (MQ=255) aaaTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAAtt < 2:485012/148‑1 (MQ=255) tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGGGTAT‑TCTACAAttttt > 1:242072/1‑148 (MQ=255) atgtCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATc > 2:414638/4‑149 (MQ=255) aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATc > 2:918261/1‑149 (MQ=255) ttCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTg < 1:295584/149‑1 (MQ=255) cGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGc > 2:1065950/1‑149 (MQ=255) gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCa < 2:349178/149‑1 (MQ=255) gggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATAGCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCAt < 2:603718/148‑1 (MQ=255) aGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCTACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCAt < 1:469951/149‑1 (MQ=255) ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATa < 1:49510/149‑1 (MQ=255) tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCatat > 1:992287/1‑149 (MQ=255) tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAAAGTACTCAGCATCTCTGTCTTTTGCGTAT‑TCTACGATTTTATCTGTTGCATAGCCatat > 1:1042780/1‑149 (MQ=255) cccTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACACTTTTATCTGTTGCATAGCCATATg > 1:169363/1‑149 (MQ=255) cccTTCTTTTCCAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTGTG‑TTATCTCTAGAATTTTATCTGTTGCATAGCCATATg < 2:1073684/149‑1 (MQ=255) ccTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCAGAGCCATATGc > 2:273433/1‑149 (MQ=255) ttttCTAACAGGGGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGTGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc < 2:831455/149‑1 (MQ=255) tctCTAACAGGTGCAAACGCAAGCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc < 1:854717/147‑1 (MQ=255) ttCTAACAGCTGCAAAGCCATCCCCCTGTGAATACCTTCAAGGACATGCTGTGATATTCCCAGGCTCTTCAGGTCCTACAACGATATCAACATCTCTCTCTTTTGCGTAT‑TCTACATTTTTATCTGTTGCATAGCCATATGCTTCACCa < 2:394131/149‑1 (MQ=255) tCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATGGCCATATGCTTCAccct > 2:1006166/1‑147 (MQ=255) aCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATc < 1:462403/149‑1 (MQ=255) cAGGTGCAAAGCCAATCCCCATTTAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc < 1:262455/149‑1 (MQ=255) aGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa < 1:137901/149‑1 (MQ=255) aGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGAACAAACAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGGTGCATAGCCATATGCTTCACCATTATCCa < 1:581780/149‑1 (MQ=255) ttcAAGGCGAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa < 2:1110872/147‑1 (MQ=255) tGCAAAGCCAATCCCCATTGAATAGGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa < 2:159492/149‑1 (MQ=255) tGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa < 2:892206/149‑1 (MQ=255) gCAAACCCACTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAt < 1:233723/149‑1 (MQ=255) aaaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACCACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCTCCATTTTTCATACttt > 1:349949/1‑148 (MQ=255) aaGCCAATCCTCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTCCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgt < 2:923817/149‑1 (MQ=255) aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgt < 1:414638/149‑1 (MQ=255) aGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgta < 1:158564/149‑1 (MQ=255) aGCCAATACCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgta < 2:1113852/149‑1 (MQ=255) cAATCCCCATTGAATAAGTTACAGGCCACCCAGTGATAAAGCCACGCGCTTCAGGTCCTGCAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagta > 2:793238/1‑149 (MQ=255) cccATTGAATAAGTTACAGGCCAGCCAATGATAAAGACAAGCGCTTCAGGTCCTACAACGGTATCAAAATCTCTGTCTTTTGCGTAT‑TCTACACTTTTATCTATTGCATAGCCATATGCTTCACCTTTATCCATAACTGTAGTAATATc > 2:117407/1‑149 (MQ=255) tttatcaaGTTACAGGACAGCCAATGATAAATCCACGCGCTTCCGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTTT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTCTCCATAATTGTAGTAATATCCTTg < 2:1064913/143‑1 (MQ=255) ttGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTg < 1:270816/149‑1 (MQ=255) aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGaaa < 1:784222/149‑1 (MQ=255) aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGaaa < 1:185560/149‑1 (MQ=255) aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGaaa < 1:17426/149‑1 (MQ=255) aTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAAc < 1:839999/149‑1 (MQ=255) | TAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAT‑TCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAAC > USA300TCH1516_ALE/1744327‑1744616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |