Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 707,909 A→T M1M (TTG→ATG) † znuC_1 ← High‑affinity zinc uptake system ATP‑binding protein ZnuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE707,9090AT98.8% 266.2 / NA 82M1M (TTG→ATG) †znuC_1High‑affinity zinc uptake system ATP‑binding protein ZnuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (36/45);  minor base G (0/1);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGG  >  USA300TCH1516_ALE/707768‑708052
                                                                                                                                             |                                                                                                                                               
aaGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTta                                                                                                                                          <  2:706485/149‑1 (MQ=255)
 aGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTGCATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCCACAATACGTTtat                                                                                                                                         >  1:456095/1‑149 (MQ=255)
   aCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAc                                                                                                                                       >  1:93161/1‑149 (MQ=255)
   aCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGAACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAc                                                                                                                                       >  1:182542/1‑149 (MQ=255)
     gtGATAAGGGGAGATTTACCAGCACCTTTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcct                                                                                                                                     <  1:116592/148‑1 (MQ=255)
        aTAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGCAAAGAAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctcct                                                                                                                                  >  2:952907/1‑149 (MQ=255)
         tAAGGTAAGATTTACCAGCACCATTCGCGCCAATTATACGATATATTTCTCCACTTACTAGTATCTATTAGGAAATGTTTTTAAGTACATGCTTAGTACCTCAATACAGATGTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTa                                                                                                                                 <  2:459183/149‑1 (MQ=255)
                   tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGGTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGtt                                                                                                                       >  1:642601/1‑149 (MQ=255)
                   tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACCTACGTTTATACCTCCTAATTAAAAGtt                                                                                                                       >  2:384152/1‑149 (MQ=255)
                      aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAg                                                                                                                    <  1:362996/149‑1 (MQ=255)
                      aCCAGCACCATTCGGGCCCATGATACCAATTATTGCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTAGTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAg                                                                                                                    <  1:54612/149‑1 (MQ=255)
                        cAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTAAAGTACATGCTTATTACCTAAAAACAGCTTCAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGc                                                                                                                  <  1:869863/149‑1 (MQ=255)
                          gCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTa                                                                                                                >  1:226366/1‑149 (MQ=255)
                           cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTCATTAAAAGTTTAGGCTaa                                                                                                               >  1:974262/1‑149 (MQ=255)
                            aCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTacc                                                                                                              >  2:64129/1‑147 (MQ=255)
                                    ggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCCATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaattaa                                                                                                      <  1:657448/149‑1 (MQ=255)
                                    ggCCCATGATACCAAGTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaattaa                                                                                                      <  1:243097/149‑1 (MQ=255)
                                     gCCCATTGTACCAATTCTTTCGCCGCGTACGGAGATACATATGAAAATGTTTTTAATGACATTCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAGACGTTTATACCTCCTAATGAAAAGTTTAGGCTAACCtaattaat                                                                                                     <  2:116284/149‑1 (MQ=255)
                                       ccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTg                                                                                                   >  1:136163/1‑149 (MQ=255)
                                           gATACCAATTATTTCGCCGCGTTCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                                                               <  2:928434/149‑1 (MQ=255)
                                                 aaTTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAAc                                                                                         <  1:133209/149‑1 (MQ=255)
                                                    tttttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACCTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTga                                                                                      <  2:160906/147‑1 (MQ=255)
                                                      tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                                                                    <  2:400629/149‑1 (MQ=255)
                                                           ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                                                               <  1:1002238/149‑1 (MQ=255)
                                                                           aTAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                               <  1:1113247/149‑1 (MQ=255)
                                                                            tAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                              >  2:867595/1‑149 (MQ=255)
                                                                             aaGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAGTTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAtt                                                             >  1:1011396/1‑149 (MQ=255)
                                                                               ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                           <  2:11942/149‑1 (MQ=255)
                                                                               ggAAATGTTTTTAAGTACATGCGTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAATTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                           <  2:308653/149‑1 (MQ=255)
                                                                                gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                                                          >  2:851356/1‑149 (MQ=255)
                                                                                gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                                                          >  1:892528/1‑149 (MQ=255)
                                                                                gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                                                          >  2:518536/1‑149 (MQ=255)
                                                                                gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGAGTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCACGTAAATTGGt                                                          >  2:1072249/1‑149 (MQ=255)
                                                                                gAAAGGTGTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAAGCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                                                          <  1:68194/149‑1 (MQ=255)
                                                                                 aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                                         <  2:974433/149‑1 (MQ=255)
                                                                                 aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                                         >  1:611702/1‑149 (MQ=255)
                                                                                 aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                                         <  1:672221/149‑1 (MQ=255)
                                                                                  aaTTTTTTTAATTACATGCTTATTTCCTAAAAATAGATTTAAATCTTTTGTTTCATACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCCAATTAATTGTATAATAAACTGAGTATATTTATCATGTCAAGTAAATTCGTGa                                                        <  2:612855/149‑1 (MQ=255)
                                                                                  aaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGa                                                        <  2:330553/149‑1 (MQ=255)
                                                                                    tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATGCCTCCTAATTAAAAGTTGAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGata                                                      <  2:399841/149‑1 (MQ=255)
                                                                                     gTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                                     <  1:342354/149‑1 (MQ=255)
                                                                                        ttttAGTACGTGGTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataat                                                  <  1:6120/149‑1 (MQ=255)
                                                                                         ttAAGTACATGTTTATTACCCAAAAACAGATTCAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGGTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata                                                 <  2:1044122/149‑1 (MQ=255)
                                                                                         ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata                                                 <  1:247589/149‑1 (MQ=255)
                                                                                         ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata                                                 <  1:135631/149‑1 (MQ=255)
                                                                                         ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata                                                 <  1:151821/149‑1 (MQ=255)
                                                                                            aGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATACACTGAGAGTAGTTATCATGTCAAGTAAATTCGTGATATAATATAg                                              >  1:307269/1‑149 (MQ=255)
                                                                                            aGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg                                              >  1:189446/1‑149 (MQ=255)
                                                                                            aGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg                                              >  1:201152/1‑149 (MQ=255)
                                                                                             gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGa                                             <  1:458067/149‑1 (MQ=255)
                                                                                             gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAGTCGTGATATAATATAGa                                             >  1:283829/1‑149 (MQ=255)
                                                                                             gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAACAAGAAAAGTAGGCTCGTGATATAATATTGa                                             <  2:712885/149‑1 (MQ=255)
                                                                                                 aTGCTTATTACCTAAAACCAGCTTGTATTGGTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAGCCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCCTGATATAATATAGACaaa                                         >  2:565828/1‑148 (MQ=255)
                                                                                                 aTGCTTATTACCTAAAAACAGATTTACATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCGAGTAAATTCGTGATATAATATAGACAat                                         <  1:675488/149‑1 (MQ=255)
                                                                                                  tGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                        >  1:565903/1‑149 (MQ=255)
                                                                                                  tGCTTATTACCTAAAAACAGATCTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                        >  2:757681/1‑149 (MQ=255)
                                                                                                        ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATCCGTGATATAATATAGACAATGTAtgtg                                  >  2:1091486/1‑149 (MQ=255)
                                                                                                          aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAGCTGAGAGTATTTATCATGTCAGGTGGATACGGGGTATAGTATAGGCGAGGTAGGTGAg                                >  1:463285/1‑149 (MQ=255)
                                                                                                          aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAg                                <  2:750759/149‑1 (MQ=255)
                                                                                                          aCCTAAAAACAGATTTAAATCTTCTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAg                                >  1:829525/1‑149 (MQ=255)
                                                                                                             tAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                             >  2:899488/1‑149 (MQ=255)
                                                                                                                aaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGaaa                          <  2:959160/149‑1 (MQ=255)
                                                                                                                  aCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAATTTCGTGATATAATATAGACAATGTATGTGAGGTGAAAgg                        >  1:840873/1‑148 (MQ=255)
                                                                                                                  aCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGCATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATCGACAATGTATGTGAGGTGAAAGt                        >  1:548288/1‑149 (MQ=255)
                                                                                                                  aCAGATTTAAATCTTCTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGCTTAGGCTAACATAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAGAGt                        >  2:785548/1‑149 (MQ=255)
                                                                                                                      aTTTAAATTTTTTGTTTCGAACATAGATTTAAACCTCCTAATTAAAAGTTTAGGCTAACCTAAGTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGt                    <  1:40772/149‑1 (MQ=255)
                                                                                                                      aTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGt                    <  1:1033768/149‑1 (MQ=255)
                                                                                                                         tAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa                 <  1:484965/149‑1 (MQ=255)
                                                                                                                         tAAATCTTTTGTTTCTAACATACGTTTATACCACCTACTTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa                 <  1:1059525/149‑1 (MQ=255)
                                                                                                                             tCTTTTGTTTCTAACATCCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga             <  1:629952/149‑1 (MQ=255)
                                                                                                                             tCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga             <  1:214579/149‑1 (MQ=255)
                                                                                                                                 ttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAGTCTATATGAGGTGAAAGTATGTTAACTgaagaa         >  1:84653/1‑149 (MQ=255)
                                                                                                                                  tGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaa        >  1:1105890/1‑149 (MQ=255)
                                                                                                                                  tGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaa        >  1:1106321/1‑149 (MQ=255)
                                                                                                                                   gTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaaa       <  1:847994/149‑1 (MQ=255)
                                                                                                                                   gTTTCTAACATACGTTTATACCTCCGAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaaa       <  2:441062/149‑1 (MQ=255)
                                                                                                                                   gTTTATAACATACGTTTATAACTCCTAATTAAAAGTTTAGGCGAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAAATGAAGaaaa       <  2:547122/149‑1 (MQ=255)
                                                                                                                                     ttCTAACATAGGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag     <  2:982220/149‑1 (MQ=255)
                                                                                                                                     ttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACATTGTATGTGAGGTGAAAGTATGTTGCCTGAAGaaaagg     >  1:17299/1‑147 (MQ=255)
                                                                                                                                     ttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag     <  2:892528/149‑1 (MQ=255)
                                                                                                                                     ttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag     >  2:1008603/1‑149 (MQ=255)
                                                                                                                                        tAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAgg  <  1:294828/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
AAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGG  >  USA300TCH1516_ALE/707768‑708052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑