Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 973,213 C→A E175* (GAG→TAG)  yitU_1 ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE973,2130CA97.6% 256.6 / NA 83E175* (GAG→TAG) yitU_15‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (46/35);  total (48/35)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATATCATTATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGA  >  USA300TCH1516_ALE/973066‑973354
                                                                                                                                                   |                                                                                                                                             
cAATATCATTATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAAATTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGCATGGTGCGCCACAGCGTCAATGCTCa                                                                                                                                              >  2:387214/1‑149 (MQ=255)
  atatCATTATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGCATGCTGCGCCCCCGCGTCGATGCTCAat                                                                                                                                            >  2:212446/1‑149 (MQ=255)
       aTTATCTTCATCACCGAATGCAATAATAATATTTCGGTCAATATTTAAAAATTGTCTAACATGCTCGATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCATCGATGCTAAatattgt                                                                                                                                       >  1:681138/1‑146 (MQ=255)
         tATCTTCATCCCCGAATGCAATTATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCg                                                                                                                                     <  1:721542/149‑1 (MQ=255)
           tCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGTTAAATAAGATGGGc                                                                                                                                   >  2:944385/1‑149 (MQ=255)
               catcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                               <  2:407612/149‑1 (MQ=255)
                  caCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaaaa                                                                                                                            <  2:1030481/149‑1 (MQ=255)
                  caCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaaaa                                                                                                                            <  2:603460/149‑1 (MQ=255)
                  caCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCAAGCGTCGATGCTAAATATGATCGGCATaaaaa                                                                                                                            >  1:24012/1‑149 (MQ=255)
                    ccGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGAGCACCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATg                                                                                                                          <  2:1071891/149‑1 (MQ=255)
                        aTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTa                                                                                                                      >  2:1094985/1‑149 (MQ=255)
                         tGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTaa                                                                                                                     <  2:529662/149‑1 (MQ=255)
                          gCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAg                                                                                                                    <  1:505190/149‑1 (MQ=255)
                          gCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAg                                                                                                                    <  1:1108599/149‑1 (MQ=255)
                                 tattatTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAttt                                                                                                             >  1:466694/1‑149 (MQ=255)
                                  attattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCGTAAAATTGAGTAAGCATAtttt                                                                                                            >  1:975265/1‑149 (MQ=255)
                                  attattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAtttt                                                                                                            >  1:835251/1‑149 (MQ=255)
                                   ttattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAttttt                                                                                                           >  2:546862/1‑149 (MQ=255)
                                     attTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGa                                                                                                         >  2:559918/1‑149 (MQ=255)
                                      tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAt                                                                                                        >  1:1099991/1‑149 (MQ=255)
                                      tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAt                                                                                                        >  2:902188/1‑149 (MQ=255)
                                       ttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCCAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAtt                                                                                                       >  1:107371/1‑149 (MQ=255)
                                       ttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAtt                                                                                                       >  1:1011579/1‑149 (MQ=255)
                                       ttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAAGGAGTAAGCATATTTTTGAtt                                                                                                       >  1:1050959/1‑149 (MQ=255)
                                        tCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTCATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAttt                                                                                                      <  1:1042548/149‑1 (MQ=255)
                                        tCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAttt                                                                                                      <  1:1042657/149‑1 (MQ=255)
                                        tCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATACCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCACAAAAATGAGTAAGCATATTTTTGAttt                                                                                                      <  1:596576/149‑1 (MQ=255)
                                          ggTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCGGCGTCGATGCTAAATATGGTCTGCATAAAAAGGAGTAAGCATATTTTTGATTTCa                                                                                                    >  1:1063959/1‑149 (MQ=255)
                                          ggTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCAAATTTTTGATTTCa                                                                                                    >  2:1097003/1‑149 (MQ=255)
                                            tCAATATTTAAAAATTGTTTAACTTGCTCCATGCCTCATGCTTTATTAATACCAAGTTTTACACTTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTCGATTTCAgg                                                                                                  >  2:163725/1‑149 (MQ=255)
                                            tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                  >  2:120455/1‑149 (MQ=255)
                                            tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                                  >  1:897791/1‑149 (MQ=255)
                                            tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGAAAGCATATTTTTGATTTTAgg                                                                                                  >  1:469336/1‑149 (MQ=255)
                                              aaTATTTAAAAATTGACTAACTTGCTCAATGCCTCTTGCTTTATTACTACCACGTTTTACAATTTCAATGACAGGGTATGGTGCGCCCCAGCGTTGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTa                                                                                                <  2:569430/149‑1 (MQ=255)
                                                  tttAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTACTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtttt                                                                                            <  1:884534/149‑1 (MQ=255)
                                                    tAAAAATTGTCTAACTTGCCCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTAc                                                                                          <  2:734296/149‑1 (MQ=255)
                                                           tGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTAAAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTAATTTCAGCTATTTTACTttcttg                                                                                   >  1:608344/1‑148 (MQ=255)
                                                              cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCACTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATGTTACTTTCTTCGGc                                                                                >  1:34241/1‑149 (MQ=255)
                                                              cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGc                                                                                >  2:714613/1‑149 (MQ=255)
                                                              cTAACTTGCTCAATGCCTCTTGACTTATTCGTGCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGc                                                                                >  1:270479/1‑149 (MQ=255)
                                                               tAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCCGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATACTTTCGATTTCCGGTATTTTACTTTCTTCGGCt                                                                               >  2:335140/1‑149 (MQ=255)
                                                               tAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCt                                                                               >  1:332261/1‑149 (MQ=255)
                                                                 aCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTc                                                                             >  1:519071/1‑149 (MQ=255)
                                                                  cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                                 >  2:807750/1‑144 (MQ=255)
                                                                  cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                                 <  1:807750/144‑1 (MQ=255)
                                                                  cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                            >  1:1028435/1‑149 (MQ=255)
                                                                  cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                            <  1:237508/149‑1 (MQ=255)
                                                                  cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAAAGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAACATGATCCGCATACCCATCCGTAAGCATATTTTTGATTTCATGTATTTTACTTTCTTCGGCTTCa                                                                            <  2:818682/149‑1 (MQ=255)
                                                                      cTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGGTTCCATTa                                                                        >  2:264131/1‑149 (MQ=255)
                                                                         aaTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaaa                                                                     <  1:177832/149‑1 (MQ=255)
                                                                         aaTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaaa                                                                     <  1:178000/149‑1 (MQ=255)
                                                                         aaTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaaa                                                                     >  1:863024/1‑149 (MQ=255)
                                                                           tGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTCAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATGAAACtt                                                                   >  2:413871/1‑149 (MQ=255)
                                                                             ccTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGa                                                                 <  1:334827/149‑1 (MQ=255)
                                                                               tctTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTTGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAgg                                                               <  2:297499/149‑1 (MQ=255)
                                                                                  tGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTGATTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAg                                                            <  2:466694/149‑1 (MQ=255)
                                                                                   gCTTTATTAATCCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAgg                                                           <  1:980623/149‑1 (MQ=255)
                                                                                      ttattaATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGGATATTTTTGATTTCAGGTAGTTTACTTTCTTCGGATTCAATTAAAATTGAGGTGggggg                                                        >  2:840643/1‑148 (MQ=255)
                                                                                      ttattaATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                        >  1:841876/1‑149 (MQ=255)
                                                                                       tattaATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGAt                                                       <  1:229931/149‑1 (MQ=255)
                                                                                            aTACCAAGTTTTACAATTTCAATGCCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCttt                                                  >  1:558693/1‑149 (MQ=255)
                                                                                            aTACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCttt                                                  <  1:541490/149‑1 (MQ=255)
                                                                                            aTACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCGAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCttt                                                  <  1:1059913/149‑1 (MQ=255)
                                                                                               ccAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATGTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTCGGGGATTCTTTCag                                               >  1:753175/1‑148 (MQ=255)
                                                                                                cAAGTTTTACAATTCCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAACTGAGTAAGCATATTTTTGATTTTACGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                              >  2:617076/1‑149 (MQ=255)
                                                                                                 aaGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTCAAATTGAGGTAGGGGCTTCTTTCAAgg                                             >  2:155714/1‑148 (MQ=255)
                                                                                                     tttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGAc                                         >  1:114245/1‑149 (MQ=255)
                                                                                                      ttACCATCTCAATGACAGGGAATGGTGCGTCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGGGTGAGCATATTTTTGCTTTCATGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACa                                        <  1:1095771/149‑1 (MQ=255)
                                                                                                       tACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACaa                                       <  2:839467/149‑1 (MQ=255)
                                                                                                        aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAg                                      <  2:59833/149‑1 (MQ=255)
                                                                                                         cAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATCAAAATGCGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGt                                     >  1:1052989/1‑149 (MQ=255)
                                                                                                              tCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTAAAGTGGAGAAGTAAAtt                                >  2:15029/1‑149 (MQ=255)
                                                                                                                 aTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTAcc                             <  2:661990/149‑1 (MQ=255)
                                                                                                                 aTGACAGGGAATGGTGCGACCCAGCTTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTAcc                             <  1:1032459/149‑1 (MQ=255)
                                                                                                                 aTGACAGGGAATGGTGAGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTAcc                             <  2:150755/149‑1 (MQ=255)
                                                                                                                    aCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGt                          >  1:1050778/1‑149 (MQ=255)
                                                                                                                    aCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGt                          >  2:1000665/1‑149 (MQ=255)
                                                                                                                       gggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAACATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTg                       >  2:852143/1‑149 (MQ=255)
                                                                                                                       gggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGCTTCTTTCAAGTGGCCACGTAAATTACCaagttg                       >  1:40579/1‑144 (MQ=255)
                                                                                                                       gggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTg                       >  1:865243/1‑149 (MQ=255)
                                                                                                                                     ccAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAc         <  1:1094985/149‑1 (MQ=255)
                                                                                                                                        gCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGGGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTTTTGGATTACCCa      <  1:762286/149‑1 (MQ=255)
                                                                                                                                            cGATGCTAAATATGATCTGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGa  <  2:1092088/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CAATATCATTATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCATTGA  >  USA300TCH1516_ALE/973066‑973354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑