Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.96.2% 346.3 / 4.5 79coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base . (46/30);  total (48/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAATAAA  >  CP000731/25524‑25802
                                                                                                                                           |                                                                                                                                           
ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGCAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACCAATaa                                                                                                                                            >  1:297399/1‑141 (MQ=255)
  gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaa                                                                                                                                          >  1:198417/1‑141 (MQ=255)
   tatGTTATTTACACTAGTTCTTAAATTTACAGGTTTGTAACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaa                                                                                                                                         <  2:136738/141‑1 (MQ=255)
     tgatATTTACATTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCAATATTTCTAATTCCGTTTAATTACTATACTACAAATaaaaaat                                                                                                                                       <  1:144906/138‑2 (MQ=255)
     tGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAGTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaat                                                                                                                                       >  1:252100/1‑140 (MQ=255)
       ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                     >  2:74946/1‑138 (MQ=255)
        tATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatata                                                                                                                                    <  1:249863/141‑5 (MQ=255)
         ttctACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATAc                                                                                                                                  <  2:33486/138‑1 (MQ=255)
           ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTTAAAATAAAAAAA‑ATACac                                                                                                                                >  2:148984/1‑140 (MQ=255)
           ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACAt                                                                                                                                >  2:8596/1‑141 (MQ=255)
            tACACTAGTTCTTAATTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATc                                                                                                                               <  1:327650/141‑1 (MQ=255)
              cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTa                                                                                                                             >  1:162094/1‑141 (MQ=255)
               acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATCCTACAAAT‑AAAAGATATACATCaaa                                                                                                                            >  1:63085/1‑141 (MQ=255)
                cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATAAATCTaaa                                                                                                                           >  2:70841/1‑141 (MQ=255)
                   gTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGt                                                                                                                        >  2:139159/1‑141 (MQ=255)
                   atGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGTTTTTCTCAATATACAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGt                                                                                                                        <  1:115881/140‑1 (MQ=255)
                     gCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATGAATCTAAAGGTTa                                                                                                                      >  2:269081/1‑141 (MQ=255)
                        tAAATTTACAGGTTTGTCCCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTGTTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAt                                                                                                                   >  2:258015/1‑141 (MQ=255)
                        tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCGATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTCAAGGTTAAAt                                                                                                                   >  2:240924/1‑141 (MQ=255)
                        tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAt                                                                                                                   >  1:96517/1‑141 (MQ=255)
                          aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCACTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCAGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTa                                                                                                                 >  2:254516/1‑141 (MQ=255)
                            tttACAGGTTTGTCACCAAGCATTGAAGGATCTTGGTTTTCTCAGTATCCAGAAATTTACTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTATAAAT‑AAATAATATACATCGAAAGGTTAAATTAAt                                                                                                               >  1:225812/1‑141 (MQ=255)
                                cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAGATTAATGaaa                                                                                                           >  1:110240/1‑141 (MQ=255)
                                  ggTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAGTAAAAAAA‑ATACATCTAAAGGTTAAATTAATTaaaaa                                                                                                         >  1:154418/1‑141 (MQ=255)
                                     ttatCACTAAGTATTGAAGGATCTTGGTTTACGCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAg                                                                                                      <  2:277902/138‑1 (MQ=255)
                                     ttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAg                                                                                                      >  1:85344/1‑141 (MQ=255)
                                     ttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAg                                                                                                      >  1:254061/1‑141 (MQ=255)
                                          aCCAACTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATATTTGTAGCACTATTTTTTATGCTGTGTAATTACTGTACTACCAAT‑CAAAAATATACAGNTACAAGTTCAATTAATNAAAAATAGaaatg                                                                                                 >  1:163024/1‑138 (MQ=255)
                                               gTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGaa                                                                                            <  2:354911/141‑1 (MQ=255)
                                                   tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                        >  2:19016/1‑141 (MQ=255)
                                                    gAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAg                                                                                       >  1:332541/1‑141 (MQ=255)
                                                     aaGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                      >  2:187001/1‑141 (MQ=255)
                                                      aGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                     >  1:153868/1‑141 (MQ=255)
                                                       ggATCTTGGTTTTCTCAATATCCAGAAATTTTCTATATAAATGTAGCACTATTTCTTATGCTATTTAAATACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaaa                                                                                    <  1:198354/141‑1 (MQ=255)
                                                            ttGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTATAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaa                                                                               <  2:317420/141‑1 (MQ=255)
                                                               gatttCTTAATATCCAGAAATTTTCTTTATAATTGTAGAACTATTTCTTATGCTGTTGAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAAAAAAAg                                                                            <  2:209797/139‑1 (MQ=255)
                                                               gTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                            <  2:363434/141‑1 (MQ=255)
                                                                   tctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAGAACAAAAAGGAAt                                                                        >  2:311319/1‑141 (MQ=255)
                                                                   tctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAt                                                                        >  2:348244/1‑141 (MQ=255)
                                                                   tctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGGTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAGTAGAATTAGAGAATATAGAAGACGAAAAGGAAt                                                                        >  1:152344/1‑141 (MQ=255)
                                                                    ctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGAAAtt                                                                       >  2:228025/1‑141 (MQ=255)
                                                                       aaTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtct                                                                    >  1:162977/1‑141 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTTTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAAAAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                                  >  2:142423/1‑141 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACACAAAGAAATTTCTCa                                                                  >  1:173248/1‑141 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                                  >  2:219314/1‑141 (MQ=255)
                                                                           tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                                <  1:74946/141‑1 (MQ=255)
                                                                                   ttttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTa                                                        <  1:8596/141‑1 (MQ=255)
                                                                                     ttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAg                                                      >  2:342342/1‑141 (MQ=255)
                                                                                      tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                     >  1:123840/1‑141 (MQ=255)
                                                                                         ttATAATTGTAGCACAAATGATTATTCGCTTTAATTACTAATCTACAAAT‑AAAAAATATACATCTAAAGATTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAATTaaa                                                  <  2:110240/141‑1 (MQ=255)
                                                                                          tataAATGTAGCACTATTTCTTATGCTGTTTTATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAg                                                 <  1:191934/141‑1 (MQ=255)
                                                                                              aTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                             <  1:89967/141‑1 (MQ=255)
                                                                                                   aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAg                                        <  2:334435/141‑1 (MQ=255)
                                                                                                     cACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtg                                      >  1:270061/1‑141 (MQ=255)
                                                                                                      aCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATATGATTAGAAACTATAGAAAACCAAAAAGAAAATCTCAAGAAACAATAAGAAAAGGAATAAAAgtgg                                     >  1:31253/1‑140 (MQ=255)
                                                                                                      aCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                     >  2:29557/1‑141 (MQ=255)
                                                                                                      aCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTACAGAATATAGAAAACAAAAAGAAATTTCTCAAGAAACTTTAATTAAAGAAATAAAAgttt                                     >  2:31253/1‑139 (MQ=255)
                                                                                                       caaatacTTAGGCTGTTTAATTACAAAACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                                    <  2:162094/135‑1 (MQ=255)
                                                                                                       cTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                                    >  2:314574/1‑141 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                                 <  2:85344/141‑1 (MQ=255)
                                                                                                             cTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                              >  2:44275/1‑141 (MQ=255)
                                                                                                             cTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                              <  1:108771/141‑1 (MQ=255)
                                                                                                             cTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAAAAGCATTAGAAAATGTGAAAAAAAAAAAGGACTTTCTAAAGAAAGTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                              >  2:224639/1‑141 (MQ=255)
                                                                                                               tATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGTAGTCAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaaa                            <  1:70841/141‑1 (MQ=255)
                                                                                                               tATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaaa                            <  1:175873/141‑1 (MQ=255)
                                                                                                               tATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaaa                            <  1:297999/141‑1 (MQ=255)
                                                                                                                  gCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTa                         <  2:198417/141‑1 (MQ=255)
                                                                                                                  gCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTa                         <  2:150753/141‑1 (MQ=255)
                                                                                                                   cctgtaaaTTACTAAACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                        <  2:182916/135‑1 (MQ=255)
                                                                                                                   cTGTTTAAATACTATACAACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                        <  1:149750/141‑1 (MQ=255)
                                                                                                                     gTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTa                      >  2:270711/1‑141 (MQ=255)
                                                                                                                      tttAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTaa                     <  1:346046/141‑1 (MQ=255)
                                                                                                                        tAATTACTATACTACAAATAAAAAAA‑AAACATCTAAAGGTTAAATGAATGAAAAATAGAATTAGAGAATATAGAAATCAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATg                   <  2:13281/141‑1 (MQ=255)
                                                                                                                          aTTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCa                 <  1:347724/141‑1 (MQ=255)
                                                                                                                           ttACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa                >  1:21724/1‑141 (MQ=255)
                                                                                                                           ttACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa                >  2:247721/1‑141 (MQ=255)
                                                                                                                            tactatactaCAAAT‑GAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt               >  1:276481/1‑141 (MQ=255)
                                                                                                                            tactatactaCAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt               >  1:100788/1‑141 (MQ=255)
                                                                                                                            taatatactaCAAAT‑AAAAAATATACATCAAAAGGTTAAATTAATGAAAAAAAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt               <  1:90780/138‑1 (MQ=255)
                                                                                                                               tatactaCAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGa            <  1:283651/141‑1 (MQ=255)
                                                                                                                               tatactaCAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGa            >  2:191924/1‑141 (MQ=255)
                                                                                                                               tatactaCAAAT‑AAAAAATATACATCTAAAGGTTAAATAAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAAAATAAAAAGAGTCTAGGAAAACTATAAATGCaaaaaa            >  2:306023/1‑137 (MQ=255)
                                                                                                                                atactaCAAAT‑AAAAAATATATATATAAAGGTTAAATTAATGAAAAATAGTATTAGAGAATATGGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGCCAAACTCTTAATGCAATAGaa           <  2:256026/141‑1 (MQ=255)
                                                                                                                                  actacAAAT‑AAAAAATATACATCTAAAGGTTAAATTACTGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAGTTAAAAGTGTAATTACAAAGTACTAATGCAATaaaaaa         >  2:278926/1‑141 (MQ=255)
                                                                                                                                  actacAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAaaaaaaaa         >  2:336374/1‑141 (MQ=255)
                                                                                                                                  actacAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaaaa         >  1:159219/1‑141 (MQ=255)
                                                                                                                                          aaaaaaaaTATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaataaa  <  1:111850/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAATAAA  >  CP000731/25524‑25802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑