Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,124,5270GC21.9% 165.7 / 23.8 64D219H (GAC→CAC) ‡lexA_2LexA repressor
Reads supporting (aligned to +/- strand):  ref base G (15/35);  new base C (0/14);  total (15/49)
Fisher's exact test for biased strand distribution p-value = 2.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGTTGCT  >  USA300TCH1516_ALE/2124378‑2124676
                                                                                                                                                     |                                                                                                                                                     
ttAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTacga                                                                                                                                                      <  3:168617/151‑1 (MQ=255)
 tAAAGATGACGAAATCATATTTGTGGAGAAAAATCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTacgac                                                                                                                                                     <  4:388000/151‑1 (MQ=255)
       tGGCGAAATCATATTTGTGGAGAAAACTCACAGTATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTa                                                                                                                                               >  4:446101/1‑151 (MQ=255)
       tGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTa                                                                                                                                               >  4:215195/1‑151 (MQ=255)
         gCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTaca                                                                                                                                             >  2:72691/1‑151 (MQ=255)
         gCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTaca                                                                                                                                             >  3:31808/1‑151 (MQ=255)
         gCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTaca                                                                                                                                             >  1:918264/1‑151 (MQ=255)
             aaTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCATTGTTGAAGATGATAGATTGACACTAGTTTCACTAAATAAAGATTACGACGATCTACACttt                                                                                                                                         <  1:174181/151‑1 (MQ=255)
              aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACtttt                                                                                                                                        <  1:601015/151‑1 (MQ=255)
                cATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTtat                                                                                                                                      <  3:109759/151‑1 (MQ=255)
                           agaAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAg                                                                                                                           <  1:507378/151‑1 (MQ=255)
                               aaCTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGtgtg                                                                                                                       <  1:1044984/151‑1 (MQ=255)
                                aCTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGa                                                                                                                      >  1:438046/1‑151 (MQ=255)
                                 cTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAg                                                                                                                     <  2:984146/151‑1 (MQ=255)
                                 cTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAg                                                                                                                     <  3:352952/151‑1 (MQ=255)
                                  tCACAATATAAAGAATGGACAAATTGGTATATTTATCATTGAAGAAGAAGCGTACGTTAAGTAAGTCTTTGTTGAAGATGATAGATTTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAgtgtagat                                                                                                                    <  2:119501/151‑5 (MQ=255)
                                   cacaATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGt                                                                                                                   >  1:499019/1‑151 (MQ=255)
                                   cacaATATAAAAAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGt                                                                                                                   <  1:744527/151‑1 (MQ=255)
                                       atatAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAAt                                                                                                               >  1:153030/1‑151 (MQ=255)
                                        tataACGACTGGACCAATTGGTATATTCATCATTGAAGAAGAAGCTTACTTTAAGTAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAAtt                                                                                                              <  2:1052353/151‑1 (MQ=255)
                                               aaTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAg                                                                                                       <  1:878046/151‑1 (MQ=255)
                                                aTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGt                                                                                                      >  1:633556/1‑151 (MQ=255)
                                                           ggTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATaaa                                                                                           >  2:961611/1‑151 (MQ=255)
                                                             tataTTCATCATTGAAGAAGAAGCGTACTTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAg                                                                                         <  3:404001/151‑1 (MQ=255)
                                                             tataTTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAg                                                                                         <  2:818045/151‑1 (MQ=255)
                                                              atatTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTCTTTTTTTTAATGATACAGAGATTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAgg                                                                                        <  2:90319/151‑1 (MQ=255)
                                                              atatTCATCATTAAAGAAGAAGTGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAgg                                                                                        <  4:380521/151‑1 (MQ=255)
                                                                atTCATCATTGAAGAAGAAGCGTACGTTAAGAGAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAg                                                                                      <  2:667628/151‑1 (MQ=255)
                                                                atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGAGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAg                                                                                      <  2:871160/151‑1 (MQ=255)
                                                                atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGGTTTTGTTTAAGATTATATGTTGACTTGATTTTGACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAg                                                                                      <  1:423605/151‑1 (MQ=255)
                                                                   catcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTCTTTATTTTAATGAAACAGAGACTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCAc                                                                                   <  2:613493/151‑1 (MQ=255)
                                                                    atcatTTAAGAAGAAGCGTACGTTAAGTAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                                  <  2:703502/151‑1 (MQ=255)
                                                                    atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                                  <  4:82920/151‑1 (MQ=255)
                                                                    atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                                  <  1:961611/151‑1 (MQ=255)
                                                                    atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                                  <  1:573402/151‑1 (MQ=255)
                                                                    atcatTGAAGAAGAAGCGTACGTTAAGAAAATCTTTATTGCAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                                  <  4:39528/151‑1 (MQ=255)
                                                                        ttGAAGAAGAAGCGTACGTTAAGATAGTCTTTGTTGAAGATGTTAGGTTGTTTTTTTTTTAATGAAACGGCGACTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCa                                                                              <  4:121171/151‑1 (MQ=255)
                                                                                   gCGTACGTTAAGAAAGTCTATATTGAAAATGATAGATTGAATTTAGTTTAAAAAAAAAAAGAAAACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCAAATGAAAAAAAAAAAat                                                                   <  4:226308/151‑1 (MQ=255)
                                                                                      tACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAatatt                                                                <  1:622104/151‑2 (MQ=255)
                                                                                        cGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGat                                                              >  2:53808/1‑151 (MQ=255)
                                                                                                   tCTTTGTTGAAGATGATAGATTGTCTCTTGTTTCACGAAACAAAGACCACCGCGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCtt                                                   <  2:304388/151‑1 (MQ=255)
                                                                                                    cTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTa                                                  >  2:549792/1‑151 (MQ=255)
                                                                                                       tgttgAAGATGATAGATTGTTTTTTGTTTAATGAAACAAAGATTACCAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                               <  4:272122/151‑1 (MQ=255)
                                                                                                       tgttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                               <  1:41180/151‑1 (MQ=255)
                                                                                                       tgttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                               <  3:446101/151‑1 (MQ=255)
                                                                                                       tgttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                               <  3:38021/151‑1 (MQ=255)
                                                                                                       tgttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                               <  3:215195/151‑1 (MQ=255)
                                                                                                       tgttgAAGATAATAGATTGACTCAAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGAGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                               <  3:278017/151‑1 (MQ=255)
                                                                                                        gttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACt                                              <  1:846554/151‑1 (MQ=255)
                                                                                                         ttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACtt                                             <  3:442491/151‑1 (MQ=255)
                                                                                                          tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                            >  1:751709/1‑151 (MQ=255)
                                                                                                          tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                            <  1:438185/151‑1 (MQ=255)
                                                                                                          tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                            >  1:48452/1‑151 (MQ=255)
                                                                                                               atgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAAc                                       <  3:169085/151‑1 (MQ=255)
                                                                                                                  atAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTat                                    <  3:161971/151‑1 (MQ=255)
                                                                                                                   tAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatg                                   <  1:940153/151‑1 (MQ=255)
                                                                                                                    aGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatga                                  <  1:53808/151‑1 (MQ=255)
                                                                                                                         gACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTAtgaatgaa                             <  3:252678/151‑1 (MQ=255)
                                                                                                                         gACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTAtgaatgaa                             <  1:85840/151‑1 (MQ=255)
                                                                                                                          aCTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAAc                            <  4:31808/151‑1 (MQ=255)
                                                                                                                           ttctAGTTTCACTAAATAAAGACTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACa                           <  4:358987/150‑1 (MQ=255)
                                                                                                                               aGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATg                       >  2:244216/1‑151 (MQ=255)
                                                                                                                                          ataaaGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTg            <  1:545211/151‑1 (MQ=255)
                                                                                                                                           caaaGATTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGc           <  2:1003150/150‑1 (MQ=255)
                                                                                                                                              aGATTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGaa        <  2:630820/151‑1 (MQ=255)
                                                                                                                                                aTTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGt      <  2:153030/151‑1 (MQ=255)
                                                                                                                                                    cgacgaTCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGTTGCt  >  2:826238/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGTTGCT  >  USA300TCH1516_ALE/2124378‑2124676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑