BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,855	A→G	5.5%	D777D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,855	0	A	G	5.5%	1264.0 / 8.1	415	D777D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (190/185); new base G (9/25); total (203/212)
Fisher's exact test for biased strand distribution p-value = 7.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

GTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAA  >  USA300TCH1516_ALE/2774765‑2774945

gTCTGAGACGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGaa                                                                                            <  1:5571498/91‑1 (MQ=255)

 tCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAAt                                                                                           >  2:2037431/1‑91 (MQ=255)

 tCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAAt                                                                                           <  1:6797574/91‑1 (MQ=255)

 tCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAAt                                                                                           >  1:1942970/1‑91 (MQ=255)

  cTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATc                                                                                          <  1:1849389/91‑1 (MQ=255)

    gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCg                                                                                        >  2:1431772/1‑91 (MQ=255)

    gAGTCGCTATCTGAGCCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCg                                                                                        <  2:5739850/91‑1 (MQ=255)

      gTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGaa                                                                                      >  1:4133793/1‑91 (MQ=255)

       tCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGCATCACTGTCTGACTCCGAAt                                                                                     >  2:5403492/1‑91 (MQ=255)

             tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTat                                                                               >  1:3455521/1‑91 (MQ=255)

             tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTat                                                                               >  2:4129613/1‑91 (MQ=255)

              cTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatc                                                                              <  2:326847/91‑1 (MQ=255)

              cTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatc                                                                              <  2:581324/91‑1 (MQ=255)

               tGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatct                                                                             >  1:2981515/1‑91 (MQ=255)

                gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctg                                                                            <  1:5223216/91‑1 (MQ=255)

                gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctg                                                                            <  1:124165/91‑1 (MQ=255)

                  gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                          <  2:4346808/91‑1 (MQ=255)

                  gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                          <  2:418663/91‑1 (MQ=255)

                   tCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaat                                                                         >  1:4420381/1‑91 (MQ=255)

                   tCTGAATCGCTGTCTGAGTCGGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                                                                                <  2:4851308/84‑1 (MQ=255)

                   tCTGAATCGCTGTCTGAGTCGGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                                                                                >  1:4851308/1‑84 (MQ=255)

                    cTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatc                                                                        <  1:984710/91‑1 (MQ=255)

                    cTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatc                                                                        <  1:4341847/91‑1 (MQ=255)

                    cTGAATCGCTGTCTGAGTCTGAGTAGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatc                                                                        <  1:1743040/91‑1 (MQ=255)

                     tGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatct                                                                       >  1:4190313/1‑91 (MQ=255)

                       aaTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctga                                                                     <  1:6092558/91‑1 (MQ=255)

                        aTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTAtctgaatctgaa                                                                    >  2:4893368/1‑91 (MQ=255)

                         tCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATctgaatctgaat                                                                   >  1:2922611/1‑91 (MQ=255)

                          cGCTGTCTGAGTCTGATTCGCAATCCGAGTCTGAGTTGCTGTCTGAATTTGAATCACTGTCTGAATCCGAATGGCGATCggaatctgtatc                                                                  >  1:3462857/1‑91 (MQ=17)

                            cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                <  1:4765993/91‑1 (MQ=255)

                             tGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCt                                                               <  1:3722186/91‑1 (MQ=255)

                              gtctgagtctgaGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTa                                                              >  1:2694090/1‑91 (MQ=255)

                              gtctgagtctgaGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTa                                                              <  2:2514476/91‑1 (MQ=255)

                              gtctgagtctgaGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTa                                                              >  1:1458971/1‑91 (MQ=255)

                               tctgagtctgagTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCCGAATCACTGTCTGACTCCGAATCGCTATCTGAATCTGAGTCGCTAt                                                             >  2:616881/1‑91 (MQ=255)

                                ctgagtctgagtCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATc                                                            >  2:3883474/1‑91 (MQ=255)

                                ctgagtctgagtCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATc                                                            >  1:3076988/1‑91 (MQ=255)

                                 tgagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATcc                                                           <  2:1555030/91‑1 (MQ=255)

                                  gggtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                          <  2:4079771/89‑1 (MQ=255)

                                  gagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                          <  1:5410422/91‑1 (MQ=255)

                                  gagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                          >  2:3080143/1‑91 (MQ=255)

                                     tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCtgaatctgaatc                                                                  >  2:3931989/1‑80 (MQ=255)

                                     tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCtgaatctgaatc                                                                  <  1:3931989/80‑1 (MQ=255)

                                     tctgagtcGCTATCCGAGTCTGAATCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                          >  2:4708381/1‑88 (MQ=255)

                                       tgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCt                                                     >  1:3497135/1‑91 (MQ=255)

                                       tgagtcGCTATACGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCt                                                     <  2:6999909/91‑1 (MQ=255)

                                        gagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTg                                                    <  2:3166648/91‑1 (MQ=255)

                                         agtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGa                                                   <  2:2154653/91‑1 (MQ=255)

                                         agtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGa                                                   <  1:513869/91‑1 (MQ=255)

                                           tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGt                                                 >  2:2385371/1‑91 (MQ=255)

                                             gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCg                                               <  2:1394278/91‑1 (MQ=255)

                                             gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCg                                               <  1:1563129/91‑1 (MQ=255)

                                              cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                              <  2:1214119/91‑1 (MQ=255)

                                              cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGc                                              >  2:1352656/1‑91 (MQ=255)

                                              cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                              <  1:4009246/91‑1 (MQ=255)

                                                aTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCACTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                            <  2:2270148/91‑1 (MQ=11)

                                                aTCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                            <  1:6629363/91‑1 (MQ=255)

                                                aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                            <  1:6288040/91‑1 (MQ=255)

                                                aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                            <  2:3432208/91‑1 (MQ=255)

                                                   cGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtct                                         >  1:1076784/1‑91 (MQ=255)

                                                     aGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctga                                       >  1:6063266/1‑91 (MQ=255)

                                                     aGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATAGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctga                                       >  1:5275341/1‑91 (MQ=255)

                                                           aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctga                                 <  1:3544876/91‑1 (MQ=255)

                                                            gTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                >  1:6339078/1‑91 (MQ=255)

                                                            gTCGCTGTCAGAATCTCAATCACTGTCTGCATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                >  1:6429458/1‑91 (MQ=255)

                                                              cGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCtgaatctgaatc                              <  1:2212915/91‑1 (MQ=255)

                                                               gCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCg                             >  2:1636112/1‑91 (MQ=255)

                                                                ccgcctgaatctgagtCACTGTCTGAGTCCGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGc                            <  2:6593352/87‑1 (MQ=14)

                                                                cTGTCTGAATCTGAATCTCTGTCTGAATCCGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGc                            <  2:6221473/91‑1 (MQ=255)

                                                                cTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGc                            >  1:133257/1‑91 (MQ=255)

                                                                  gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTg                          <  2:5086426/91‑1 (MQ=255)

                                                                   tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGt                         >  1:1126016/1‑91 (MQ=255)

                                                                      gatcctgagtcGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGGGTCGCTGTCTGAATCTGAATCGCTGTCTg                      <  2:4973778/87‑1 (MQ=14)

                                                                      gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTg                      >  1:2589055/1‑91 (MQ=255)

                                                                        atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                    <  1:340630/91‑1 (MQ=255)

                                                                         tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGt                   >  2:4411100/1‑91 (MQ=255)

                                                                         tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                   >  2:458369/1‑91 (MQ=255)

                                                                          ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTc                  >  2:2866019/1‑91 (MQ=255)

                                                                          ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTc                  >  1:5745265/1‑91 (MQ=255)

                                                                          ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTc                  >  2:771391/1‑91 (MQ=255)

                                                                           tgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTcc                 <  1:762240/91‑1 (MQ=255)

                                                                            gaatcACTGTCTGAATCCGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                <  2:3238456/91‑1 (MQ=255)

                                                                            gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                <  1:4576079/91‑1 (MQ=255)

                                                                              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGaa              >  2:3529766/1‑91 (MQ=255)

                                                                              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa              >  2:5235776/1‑91 (MQ=255)

                                                                              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa              >  1:2094020/1‑91 (MQ=255)

                                                                               tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAAt             >  2:261289/1‑91 (MQ=255)

                                                                                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATc            >  1:2008456/1‑91 (MQ=255)

                                                                                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATc            <  1:1045601/91‑1 (MQ=255)

                                                                                  cTGTCTGAGTCCGAATCGCTAGCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGc          <  2:6362956/91‑1 (MQ=255)

                                                                                   tGTCTGAATCCGAATCGCTATCTGACTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCt         <  2:5341973/91‑1 (MQ=255)

                                                                                    gTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCtg        >  2:5564280/1‑90 (MQ=255)

                                                                                    gTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa        >  1:2439571/1‑91 (MQ=255)

                                                                                    gTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa        >  2:5909928/1‑91 (MQ=255)

                                                                                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTat       >  2:898664/1‑91 (MQ=255)

                                                                                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc      >  1:4995480/1‑91 (MQ=255)

                                                                                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc      <  1:5961199/91‑1 (MQ=255)

                                                                                       tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatct     >  2:1023841/1‑91 (MQ=255)

                                                                                        gAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctg    <  1:3331992/91‑1 (MQ=255)

                                                                                        gAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctg    <  1:6186257/91‑1 (MQ=255)

GTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAA  >  USA300TCH1516_ALE/2774765‑2774945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`