BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,416,458	A→T	I216N (ATT→AAT)	oppD_4 ←	Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,416,458	0	A	T	100.0%	1396.5 / NA	356	I216N (ATT→AAT)	oppD_4	Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (156/200); total (156/200)

Only 100 of 357 total aligned reads displayed.

TAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAT  >  USA300TCH1516_ALE/1416370‑1416548

tAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCACTGATTTGACCTTCTTTTAAAACATAATTg                                                                                           >  2:312113/1‑91 (MQ=255)

   aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAc                                                                                        >  1:4185766/1‑91 (MQ=255)

   aCGTTCAGTATACGGATGCAAATGCTCATACTTAAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAc                                                                                        >  1:6994964/1‑91 (MQ=255)

    cGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACt                                                                                       <  2:5679477/91‑1 (MQ=255)

      ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                     >  1:6726167/1‑91 (MQ=255)

      ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                     <  2:6848251/91‑1 (MQ=255)

      ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                     >  1:4777490/1‑91 (MQ=255)

       tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAc                                                                                    >  1:6839091/1‑91 (MQ=255)

        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                   >  1:1216870/1‑91 (MQ=255)

        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                   <  2:6133591/91‑1 (MQ=255)

        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                   >  2:5496269/1‑91 (MQ=255)

        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                   <  1:5296300/91‑1 (MQ=255)

        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                   <  1:5093044/91‑1 (MQ=255)

          gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                 >  1:6486929/1‑91 (MQ=255)

          gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                 >  2:2842929/1‑91 (MQ=255)

               cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑Tc                                                                           >  1:4647250/1‑91 (MQ=255)

                    gCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATAc                                                                      >  2:4073014/1‑91 (MQ=255)

                    gCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATAc                                                                      >  2:4803891/1‑91 (MQ=255)

                    gCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATAc                                                                      >  1:568772/1‑91 (MQ=255)

                     cAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACa                                                                     <  1:6294455/91‑1 (MQ=255)

                     cAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACa                                                                     <  2:5952141/91‑1 (MQ=255)

                      aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACAt                                                                    >  1:701757/1‑91 (MQ=255)

                       aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATg                                                                   >  1:3726293/1‑91 (MQ=255)

                        aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGa                                                                  <  2:1546118/91‑1 (MQ=255)

                         tGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGAc                                                                 >  1:6919185/1‑91 (MQ=255)

                          gCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACt                                                                <  1:3017633/91‑1 (MQ=255)

                          gCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACt                                                                <  1:1763071/91‑1 (MQ=255)

                           cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACtt                                                               <  1:5231926/91‑1 (MQ=255)

                           cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACtt                                                               >  2:1968326/1‑91 (MQ=255)

                            tCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTa                                                              <  1:1080179/91‑1 (MQ=255)

                             cATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTaa                                                             <  2:909893/91‑1 (MQ=255)

                             cATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTaa                                                             <  1:1144095/91‑1 (MQ=255)

                              aTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAg                                                            >  2:1317035/1‑91 (MQ=255)

                              aTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAg                                                            >  2:2722112/1‑91 (MQ=255)

                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGt                                                           <  1:4364249/91‑1 (MQ=255)

                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGt                                                           <  1:4026557/91‑1 (MQ=255)

                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGt                                                           <  2:1770186/91‑1 (MQ=255)

                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGt                                                           <  1:2659761/91‑1 (MQ=255)

                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGt                                                           <  1:6154728/91‑1 (MQ=255)

                               tACTCGAGATTATTCATATTAACTCGTTCATTTATTTGACCTTCTTTAATAACATAATTGTACTGACAATATT‑TCAATACAGGACTTAAGt                                                           <  2:5430429/91‑1 (MQ=255)

                                aCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGtt                                                          >  2:2247900/1‑91 (MQ=255)

                                   tGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTAtg                                                       <  1:992804/91‑1 (MQ=255)

                                     aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTAtgtg                                                     <  2:631197/91‑1 (MQ=255)

                                     aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTAtgtg                                                     <  2:5757126/91‑1 (MQ=255)

                                       aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTAtgtgtg                                                   >  2:1206073/1‑91 (MQ=255)

                                       aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTAtgtgtg                                                   >  1:6032089/1‑91 (MQ=255)

                                        tGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAAT‑TCAATACATGACTTAAGTTATGTGTGa                                                  <  1:2528052/91‑1 (MQ=255)

                                         gATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGat                                                 <  1:3807443/91‑1 (MQ=255)

                                         gATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGat                                                 >  1:451627/1‑91 (MQ=255)

                                           ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGataa                                               >  1:2910780/1‑91 (MQ=255)

                                           ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGataa                                               >  2:3743776/1‑91 (MQ=255)

                                             aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGataata                                             >  2:4599169/1‑91 (MQ=255)

                                              atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGAtaataa                                            <  2:76405/91‑1 (MQ=255)

                                               tactACATCGTTCAATGACTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATaataaa                                           <  1:5889556/88‑1 (MQ=255)

                                                 ttACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATaataaata                                         <  1:1619510/91‑1 (MQ=255)

                                                 ttACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATaataaata                                         <  1:127336/91‑1 (MQ=255)

                                                  tACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAataaataa                                        <  1:4873294/91‑1 (MQ=255)

                                                   aCCTCGTTCAATGATTTGACCTTCTTTTAAAACATCATTGTACTGCCAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAaataat                                       >  2:6769078/1‑91 (MQ=255)

                                                    ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                      <  2:1490567/91‑1 (MQ=255)

                                                    ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                      >  2:3508312/1‑91 (MQ=255)

                                                    ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGCTATGTGTGATAATAAATAATg                                      >  2:5172201/1‑91 (MQ=255)

                                                       cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGttt                                   >  2:1381335/1‑91 (MQ=255)

                                                        gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                  <  2:4884944/91‑1 (MQ=255)

                                                        gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                  <  1:6539531/91‑1 (MQ=255)

                                                        gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TAAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                  <  1:1962708/91‑1 (MQ=255)

                                                         ttCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                 <  2:1837769/91‑1 (MQ=255)

                                                         ttCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                 >  1:3508377/1‑91 (MQ=255)

                                                           cAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                               >  1:3619646/1‑91 (MQ=255)

                                                           cAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                               <  1:2159271/91‑1 (MQ=255)

                                                            aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                              <  2:1880187/91‑1 (MQ=255)

                                                             aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                             <  1:2337143/91‑1 (MQ=255)

                                                               gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                           >  1:3389379/1‑91 (MQ=255)

                                                               gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                           >  2:3443609/1‑91 (MQ=255)

                                                                aTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                          <  1:851027/91‑1 (MQ=255)

                                                                 tttGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTc                         >  1:1702813/1‑91 (MQ=255)

                                                                  ttGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAGTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCt                        >  2:6493481/1‑91 (MQ=255)

                                                                  ttGACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCt                        >  1:1433295/1‑91 (MQ=255)

                                                                    gACCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTaa                      <  2:6927909/91‑1 (MQ=255)

                                                                     aCCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                     >  2:4537284/1‑91 (MQ=255)

                                                                     aCCTTCTTTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                     <  1:2477714/91‑1 (MQ=255)

                                                                       cttcttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                   <  1:5488735/91‑1 (MQ=255)

                                                                       cttcttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                   >  1:4244625/1‑91 (MQ=255)

                                                                       cttcttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                   <  1:4820925/91‑1 (MQ=255)

                                                                        ttcttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                  >  2:4374249/1‑91 (MQ=255)

                                                                         tcttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                 <  1:4693733/91‑1 (MQ=255)

                                                                          cttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAat                >  2:3056621/1‑91 (MQ=255)

                                                                          cttTTAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAat                >  1:1401044/1‑91 (MQ=255)

                                                                           ttttAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata               <  2:4477926/91‑1 (MQ=255)

                                                                           ttttAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata               <  2:2181136/91‑1 (MQ=255)

                                                                           ttttAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata               <  1:5793242/91‑1 (MQ=255)

                                                                              tAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                             >  2:6648911/1‑74 (MQ=255)

                                                                              tAAAACATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGc            <  1:2959145/91‑1 (MQ=255)

                                                                               aaaaCATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCt           <  2:23269/91‑1 (MQ=255)

                                                                                aaaCATAATTGTACTGACAATCTT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTg          >  2:244884/1‑91 (MQ=255)

                                                                                aaaCATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTg          >  2:305610/1‑91 (MQ=255)

                                                                                 aaCATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt         >  1:2070323/1‑91 (MQ=255)

                                                                                  aCATAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGta        <  1:398468/91‑1 (MQ=255)

                                                                                    aTAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaat      >  1:3125365/1‑91 (MQ=255)

                                                                                     tAATTGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaata     >  2:1432540/1‑91 (MQ=255)

                                                                                        aTGTATTGA‑AATATTGTCAGTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt  >  2:2998665/1‑91 (MQ=38)

TAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATT‑TCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAT  >  USA300TCH1516_ALE/1416370‑1416548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`