New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 2494792140 (1.120)4 (0.040) 3/236 NT 3.0% intergenic (‑155/+52) USA300HOU_RS12845/mnmC_2 hypothetical protein/tRNA 5‑methylaminomethyl‑2‑thiouridine biosynthesis bifunctional protein MnmC
?USA300TCH1516_ALE = 2494816 128 (1.120)intergenic (‑179/+28) USA300HOU_RS12845/mnmC_2 hypothetical protein/tRNA 5‑methylaminomethyl‑2‑thiouridine biosynthesis bifunctional protein MnmC
Rejected: Frequency below/above cutoff threshold.

AATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/2494642‑2494792
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aatagctatacAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTTATAATAGG  <  USA300TCH1516_ALE/2494816‑2494677
                                                                                                                                                                                                                                                                                                   
AATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAA                                                                                                                                                        >  3:396602/1‑141
 ATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTCTGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAA                                                                                                                                                       >  2:532404/1‑141
 ATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTGAAATAACTTTACATCAAGCGCTATACAAATAAAA                                                                                                                                                       >  4:348481/1‑141
 ATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAA                                                                                                                                                       >  1:781684/1‑141
 ATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAA                                                                                                                                                       >  3:130630/1‑141
   CCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATA                                                                                                                                                     >  1:84380/1‑141
   CCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATA                                                                                                                                                     >  1:881640/1‑141
     TCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGC                                                                                                                                                   <  2:977192/141‑1
        CTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTAT                                                                                                                                                >  2:501499/1‑141
         TCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATA                                                                                                                                               >  1:511519/1‑141
                                            ACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTAT                                                                                                            <  2:255489/141‑1
                                              AGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTT                                                                                                          <  2:510568/141‑1
                                              AGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTT                                                                                                          <  3:173740/141‑1
                                                                                       TAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCAT                                                                 <  3:28007/141‑1
                                                                                                                                            AATAGCTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTG            >  2:809118/1‑141
                                                                                                                                              TAGCTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTT          >  4:2435/1‑141
                                                                                                                                                 CTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTTATA       >  2:561051/1‑141
                                                                                                                                                 CTATACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTTATA       >  2:560975/1‑141
                                                                                                                                                    TACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTTTCACTGTTATAATA    >  4:228865/1‑141
                                                                                                                                                    TACAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTTATAATA    <  1:384859/141‑1
                                                                                                                                                      CAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTTATAATAGG  <  2:695045/141‑1
                                                                                                                                                                                                                                                                                                   
AATCCTCCCTCACCTTTTCATTATATCCGATTATACCTATTATAACAGTGACAAAAAAGCGAAGATATGACACAACTGAATTTGGTTTAGCACATAGTATGACAGCTAAAATAACTTTACATCAAGCGCTATACAAATAAAATAGCTATAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/2494642‑2494792
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aatagctatacAAGACGTTTGTAGCGAGCGTCATGGTATAGCTATTTTATTTGTATAGCGCTTGATGTAAAGTTATTTTAGCTGTCATACTATGTGCTAAACCAAATTCAGTTGTGTCATATCTTCGCTTTTTTGTCACTGTTATAATAGG  <  USA300TCH1516_ALE/2494816‑2494677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.