| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,104,230 | G→A | 5.5% | A212A (GCG→GCA) | pdhB → | Pyruvate dehydrogenase E1 component subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,104,230 | 0 | G | A | 5.5% | 429.3 / 7.4 | 136 | A212A (GCG→GCA) | pdhB | Pyruvate dehydrogenase E1 component subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (64/64); new base A (3/5); total (67/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 136 total aligned reads displayed.
ACCCAGTCGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAGTTG > USA300TCH1516_ALE/1104091‑1104369 | aCCCAGTCGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGa > 2:253218/1‑141 (MQ=255) ccAGTCGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATg < 1:184837/141‑1 (MQ=255) ccAGTCGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATg > 3:305235/1‑141 (MQ=255) cAGTCGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATgg > 1:886006/1‑141 (MQ=255) cGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCa < 2:87764/141‑1 (MQ=255) cGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCa < 2:43091/141‑1 (MQ=255) gTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCaa < 1:220666/141‑1 (MQ=255) taCTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGaa > 2:521438/1‑141 (MQ=255) taCTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGaa > 1:911020/1‑141 (MQ=255) aCTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAAt < 2:1063238/141‑1 (MQ=255) tAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCaa < 1:742794/141‑1 (MQ=255) atGAAATTGTGTCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATGCAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCt > 3:62791/1‑141 (MQ=255) atGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCACGAATCAATGAAAGCt > 1:899518/1‑141 (MQ=255) atGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCt > 1:402790/1‑141 (MQ=255) atGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCt < 3:86/141‑1 (MQ=255) atGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCt > 2:678352/1‑141 (MQ=255) atGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTACAAGAATCAATGAAAGCt > 3:146612/1‑141 (MQ=255) tGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTg > 3:263221/1‑141 (MQ=255) gAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGc < 1:778436/141‑1 (MQ=255) aTTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCaga < 2:357930/141‑1 (MQ=255) gTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCagaaga < 1:787330/141‑1 (MQ=255) gTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCagaaga > 1:1065838/1‑141 (MQ=255) tATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCAATGGTTCAAGAATCAATGAAAGCTGCAgaagaa > 2:953580/1‑141 (MQ=255) aTCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAAc < 1:662281/141‑1 (MQ=255) aTCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAAc > 2:342187/1‑141 (MQ=255) tCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTACAAGAATCAATGAAAGCTGCAGAAGAACt > 2:85073/1‑141 (MQ=255) gTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTg < 4:302177/141‑1 (MQ=255) gTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCAAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAAAAGAAcata < 2:725969/141‑4 (MQ=255) ttCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGa < 3:271562/141‑1 (MQ=255) ttCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCAATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGa < 4:123507/141‑1 (MQ=255) tCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGaa > 2:169011/1‑141 (MQ=255) tCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGaa > 2:925324/1‑141 (MQ=255) aTTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGaaaa < 3:356641/141‑1 (MQ=255) tCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAg < 1:76214/141‑1 (MQ=255) ccGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGa > 1:858675/1‑141 (MQ=255) gTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATg < 2:856821/141‑1 (MQ=255) gTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATg < 2:945136/141‑1 (MQ=255) tGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATgg > 1:560089/1‑141 (MQ=255) tGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATgg < 2:469244/141‑1 (MQ=255) gaagaaGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGt < 3:11336/141‑1 (MQ=255) gaagaaGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGATAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGt < 2:700585/141‑1 (MQ=255) gaagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGCAGAACTTGAAAAAGATGGTTAt < 3:182913/141‑1 (MQ=255) gaagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTAt < 1:499361/141‑1 (MQ=255) gaagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTAt > 3:421696/1‑141 (MQ=255) gaagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTAt < 3:250330/141‑1 (MQ=255) gaagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTAt < 1:942038/141‑1 (MQ=255) gaagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCACAAGAACTTGAAAAAGATGCTTAt > 2:998834/1‑141 (MQ=255) aagTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTAtt < 2:566194/141‑1 (MQ=255) gTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCt > 3:359382/1‑141 (MQ=255) tACCTGAAGAAGAATATACAATTGACAATGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGNTATTCTg > 2:816313/1‑141 (MQ=255) ccTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGtt < 3:14262/141‑1 (MQ=255) ccTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCAATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGtt < 3:1971/141‑1 (MQ=255) tGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGa > 2:660070/1‑141 (MQ=255) gaagaagaaTATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGaa > 2:513708/1‑141 (MQ=255) aagaaTATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAGGAACTTGAAAAAGATGGTTATTCTGTTGAAGTaa > 1:1005016/1‑141 (MQ=255) taCAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACtt < 2:1029208/141‑1 (MQ=255) aCAATTGACATTGGTAAGGCTAATGTGAAAAAAGACGGTAATGACATTTCAATCATCACATACGGTGCAATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTa > 2:370076/1‑141 (MQ=255) cAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTAc < 4:338677/141‑1 (MQ=255) aTTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGt < 1:949702/141‑1 (MQ=255) aCATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTg > 2:831882/1‑141 (MQ=255) cATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGt < 2:578318/141‑1 (MQ=255) aTTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGtt < 1:833567/141‑1 (MQ=255) aTTGGTAAGGAAAAAAAGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCAATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGtt < 4:384325/141‑1 (MQ=255) ggTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCaa > 1:254614/1‑141 (MQ=255) ggTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTGCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCaa < 3:220102/141‑1 (MQ=255) aGGTTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGTATCAATGAAAGCTGGAAAAAAAAAAAAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCaa < 4:331899/141‑1 (MQ=255) gCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATc < 2:729070/141‑1 (MQ=255) tgAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTg > 1:906819/1‑141 (MQ=255) aaaaaaGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGac < 2:41381/141‑1 (MQ=255) aaaaaaGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGac < 1:805795/141‑1 (MQ=255) aaaaaaGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGac > 2:779860/1‑141 (MQ=255) aaaaaaGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGac > 1:638469/1‑141 (MQ=255) aaaaaGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGaca > 1:591885/1‑141 (MQ=255) aaaaaGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGaca > 2:690607/1‑141 (MQ=255) aagaagGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACaa < 1:991553/141‑1 (MQ=255) agaagGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAAt > 2:650565/1‑141 (MQ=255) aagGTAATGACATTTCAATCATCACATACGGTGCAATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTg < 4:216289/141‑1 (MQ=255) agGTAATGACATTTCGATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGTAACAGATGGTTATTCTGTTGATGTAATTGACTTACGTACTGTTTAACTAATCGATGTTGACACAATTGt > 4:17695/1‑141 (MQ=255) agGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGt > 4:111863/1‑141 (MQ=255) ggTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTa > 1:428459/1‑141 (MQ=255) ggTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTa > 1:394280/1‑141 (MQ=255) ggTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTa > 1:390084/1‑141 (MQ=255) tAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGc > 1:323543/1‑141 (MQ=255) tAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGc < 1:251934/141‑1 (MQ=255) tAATGACATTTCAATCATCACATACGATGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGc < 2:861067/141‑1 (MQ=255) aTGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCtt > 2:264600/1‑141 (MQ=255) gACAGTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCa < 4:426204/141‑1 (MQ=255) tcaCATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTg > 2:653343/1‑141 (MQ=255) tcaCATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTg > 2:653013/1‑141 (MQ=255) tcaCATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTg > 4:167564/1‑141 (MQ=255) acaTACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGt < 3:149406/141‑1 (MQ=255) acaTACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGt < 1:439082/141‑1 (MQ=255) tACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGt > 1:1011728/1‑141 (MQ=255) aCGTTGCGATGGTTCAAGAAGCAATGAAAGCTGCAGAAGAACTTGACAAAGATGGTTATTCTGTTGAAGTACTTGACTTACGTACTGTTCAACCCATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTg < 2:271168/141‑1 (MQ=255) cGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGc < 1:723414/141‑1 (MQ=255) gTGCAATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAg > 2:576841/1‑141 (MQ=255) tGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAGt < 2:160815/141‑1 (MQ=255) gCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAGtt < 2:820865/141‑1 (MQ=255) gCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAGtt > 2:1077529/1‑141 (MQ=255) cGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTAATGAGCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAGTTg > 1:19805/1‑141 (MQ=255) | ACCCAGTCGTATACTTAGAGCATATGAAATTGTATCGTTCATTCCGTGAAGAAGTACCTGAAGAAGAATATACAATTGACATTGGTAAGGCTAATGTGAAAAAAGAAGGTAATGACATTTCAATCATCACATACGGTGCGATGGTTCAAGAATCAATGAAAGCTGCAGAAGAACTTGAAAAAGATGGTTATTCTGTTGAAGTAATTGACTTACGTACTGTTCAACCAATCGATGTTGACACAATTGTAGCTTCAGTTGAAAAAACTGGTCGTGCAGTTG > USA300TCH1516_ALE/1104091‑1104369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |