Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,190,794 A→G 6.9% A167A (GCA→GCG mraY → Phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,190,7940AG 6.9% 292.7 / 7.6 98A167A (GCA→GCGmraYPhospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base A (34/57);  new base G (4/3);  total (38/60)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TATTCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGG  >  USA300TCH1516_ALE/1190655‑1190934
                                                                                                                                           |                                                                                                                                            
tatTCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAg                                                                                                                                             <  1:252126/141‑1 (MQ=255)
 atTCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGt                                                                                                                                            <  2:499246/141‑1 (MQ=255)
  ttCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTa                                                                                                                                           <  4:353870/141‑1 (MQ=255)
    cTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTaaa                                                                                                                                         >  1:611715/1‑141 (MQ=255)
    cTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTaaa                                                                                                                                         <  1:395427/141‑1 (MQ=255)
     tttCTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAAt                                                                                                                                        <  2:647039/141‑1 (MQ=255)
        gTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTa                                                                                                                                     >  3:88715/1‑141 (MQ=255)
          tttAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAAc                                                                                                                                   <  2:235941/141‑1 (MQ=255)
           ttAAGTAATATGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACa                                                                                                                                  <  2:634469/141‑1 (MQ=255)
            tAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTTTTTTTTTTGTTTTTCTAATGCGGTAAATTTAACAg                                                                                                                                 >  4:280026/1‑141 (MQ=255)
             aaGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGa                                                                                                                                >  1:919578/1‑141 (MQ=255)
              aGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGAt                                                                                                                               >  4:185058/1‑141 (MQ=255)
               gTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATg                                                                                                                              >  1:703530/1‑141 (MQ=255)
               gTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATg                                                                                                                              >  2:1063670/1‑141 (MQ=255)
                  aTGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGtt                                                                                                                           <  3:151962/141‑1 (MQ=255)
                  aTGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGtt                                                                                                                           <  2:963959/141‑1 (MQ=255)
                     tgtTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAg                                                                                                                        >  3:445677/1‑141 (MQ=255)
                      gtTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGAAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGa                                                                                                                       <  1:586482/141‑1 (MQ=255)
                         tCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATgg                                                                                                                    <  1:102009/141‑1 (MQ=255)
                           aTTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGAt                                                                                                                  <  1:113857/141‑1 (MQ=255)
                               ggTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGc                                                                                                              <  2:461588/141‑1 (MQ=255)
                                gTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCa                                                                                                             >  2:46397/1‑141 (MQ=255)
                                gTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCa                                                                                                             >  4:193841/1‑141 (MQ=255)
                                  gAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAAc                                                                                                           >  4:249572/1‑141 (MQ=255)
                                  gAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAAc                                                                                                           <  1:828505/141‑1 (MQ=255)
                                    aTTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTg                                                                                                         <  2:530465/141‑1 (MQ=255)
                                    aTTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTg                                                                                                         <  1:68205/141‑1 (MQ=255)
                                     tttttCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTgg                                                                                                        >  3:210353/1‑141 (MQ=255)
                                     tttttCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTgg                                                                                                        >  2:197633/1‑141 (MQ=255)
                                      ttttCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGa                                                                                                       <  1:286616/141‑1 (MQ=255)
                                        ttCTACGAACATACATATTCCATTTACGAATGTAGCAATCCCACTATAATTTGCATATGTTATTTTCATTGTTTTTTGGCAATTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACt                                                                                                     >  2:497728/1‑141 (MQ=255)
                                         tCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTg                                                                                                    <  1:966040/141‑1 (MQ=255)
                                                   tacataTTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCg                                                                                          <  2:826993/141‑1 (MQ=255)
                                                    acataTTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCgg                                                                                         >  2:926964/1‑141 (MQ=255)
                                                        atTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAACTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGAttt                                                                                     <  3:349600/141‑1 (MQ=255)
                                                         ttCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGTTTAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTa                                                                                    >  2:805007/1‑141 (MQ=255)
                                                         ttCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTa                                                                                    >  1:250939/1‑141 (MQ=255)
                                                          tCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTAc                                                                                   <  1:422583/141‑1 (MQ=255)
                                                            cATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACaa                                                                                 <  2:1070769/141‑1 (MQ=255)
                                                             aTTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAAt                                                                                >  2:726628/1‑141 (MQ=255)
                                                                  cGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATg                                                                           <  1:786016/141‑1 (MQ=255)
                                                                  cGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATg                                                                           >  3:241026/1‑141 (MQ=255)
                                                                     aTGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCca                                                                        <  3:187908/141‑1 (MQ=255)
                                                                     aTGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCca                                                                        <  2:902766/141‑1 (MQ=255)
                                                                     aTGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCAACGGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTCCAATGTATGCca                                                                        <  1:102262/141‑1 (MQ=255)
                                                                         aGCAATGCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCcatcat                                                                    <  2:569634/141‑1 (MQ=255)
                                                                         aGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCcatcat                                                                    <  1:658805/141‑1 (MQ=255)
                                                                         aGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCcatcat                                                                    <  1:658776/141‑1 (MQ=255)
                                                                         aGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCcatcat                                                                    <  1:874047/141‑1 (MQ=255)
                                                                         aGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCcatcat                                                                    <  1:267403/141‑1 (MQ=255)
                                                                         aGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCcatcat                                                                    <  4:330565/141‑1 (MQ=255)
                                                                          gCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATg                                                                   <  1:621713/141‑1 (MQ=255)
                                                                           cAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGa                                                                  >  1:1070870/1‑141 (MQ=255)
                                                                            aaTCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAg                                                                 >  1:666878/1‑141 (MQ=255)
                                                                            aaTCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAg                                                                 <  2:844095/141‑1 (MQ=255)
                                                                             aTCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGc                                                                <  3:152991/141‑1 (MQ=255)
                                                                             aTCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGc                                                                >  1:816205/1‑141 (MQ=255)
                                                                               cccACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGGTTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCtt                                                              <  2:309759/141‑1 (MQ=255)
                                                                                ccACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCttt                                                             <  3:237933/141‑1 (MQ=255)
                                                                                ccACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCttt                                                             <  1:992512/141‑1 (MQ=255)
                                                                                    tATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTgtgt                                                         >  2:516204/1‑141 (MQ=255)
                                                                                    tATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTgtgt                                                         <  3:249572/141‑1 (MQ=255)
                                                                                     aTCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGtt                                                        <  1:778347/141‑1 (MQ=255)
                                                                                      tCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTa                                                       >  3:136946/1‑141 (MQ=255)
                                                                                        aTTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAgg                                                     <  2:979761/141‑1 (MQ=255)
                                                                                             cATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCGGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGaaa                                                >  1:666340/1‑141 (MQ=255)
                                                                                             cATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAGATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCTATCATGAGCTTTGTGTTATGAGaaa                                                >  2:131178/1‑141 (MQ=255)
                                                                                             cATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGaaa                                                <  2:873972/141‑1 (MQ=255)
                                                                                                  gTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCa                                           <  2:605321/141‑1 (MQ=255)
                                                                                                       tttCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTgg                                      <  2:988111/141‑1 (MQ=255)
                                                                                                       tttCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTgg                                      <  1:1002344/141‑1 (MQ=255)
                                                                                                        ttCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGTACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGt                                     <  1:43290/141‑1 (MQ=255)
                                                                                                           aTTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTAtt                                  <  1:348377/141‑1 (MQ=255)
                                                                                                           aTTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTAtt                                  <  2:765739/141‑1 (MQ=255)
                                                                                                              gTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTc                               <  1:710501/141‑1 (MQ=255)
                                                                                                                tttttGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTg                             >  1:991082/1‑141 (MQ=255)
                                                                                                                  tttGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTa                           >  1:659982/1‑141 (MQ=255)
                                                                                                                    tGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATc                         >  3:353879/1‑141 (MQ=255)
                                                                                                                     ggCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCa                        >  2:1038451/1‑141 (MQ=255)
                                                                                                                       cAAGTAGGTTTTTCTAATGCAGTAAATTAAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCAtt                      <  2:631060/141‑1 (MQ=255)
                                                                                                                        aaGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGtt                                                                                                                           >  1:182569/1‑39 (MQ=255)
                                                                                                                        aaGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGtt                                                                                                                           <  2:182569/39‑1 (MQ=255)
                                                                                                                              gTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTAtgttgt               >  1:216614/1‑141 (MQ=255)
                                                                                                                              gTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTAtgttgt               >  3:290022/1‑141 (MQ=255)
                                                                                                                              gTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTAtgttgt               >  3:289733/1‑141 (MQ=255)
                                                                                                                                ttttCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGCGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGttt             <  1:29193/141‑1 (MQ=255)
                                                                                                                                 tttCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTg            >  1:391129/1‑141 (MQ=255)
                                                                                                                                 tttCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTg            <  1:280087/141‑1 (MQ=255)
                                                                                                                                 tttCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTGGTTTg            >  3:206685/1‑141 (MQ=255)
                                                                                                                                   tCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCa          >  3:368356/1‑141 (MQ=255)
                                                                                                                                    cTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCAc         >  1:142495/1‑141 (MQ=255)
                                                                                                                                     tAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACt        <  1:519265/141‑1 (MQ=255)
                                                                                                                                       ttgtaGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACttt      >  2:580893/5‑141 (MQ=255)
                                                                                                                                       aTGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACttt      <  2:153582/141‑1 (MQ=255)
                                                                                                                                        tGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACtttt     <  1:466510/141‑1 (MQ=255)
                                                                                                                                         gCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTa    <  1:642375/141‑1 (MQ=255)
                                                                                                                                          cAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAg   <  3:185058/141‑1 (MQ=255)
                                                                                                                                          cAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAg   <  1:625219/141‑1 (MQ=255)
                                                                                                                                           aGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAgg  <  3:369086/141‑1 (MQ=255)
                                                                                                                                           aGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAgg  <  2:703530/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TATTCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATTCCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGGCAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGACTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGG  >  USA300TCH1516_ALE/1190655‑1190934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: