| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,250,301 | C→G | 6.6% | T24R (ACA→AGA) | ftsY → | Signal recognition particle receptor FtsY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,250,301 | 0 | C | G | 6.6% | 406.4 / 16.8 | 121 | T24R (ACA→AGA) | ftsY | Signal recognition particle receptor FtsY |
| Reads supporting (aligned to +/- strand): ref base C (61/52); new base G (6/2); total (67/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
TCAGGTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTT > USA300TCH1516_ALE/1250163‑1250439 | acatccattACTAAACGTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACag < 2:585071/134‑1 (MQ=255) cAGGTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACaga > 4:133253/1‑141 (MQ=255) ggTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTAAAGGAGGCGCAATTATGCCCTTCTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGagaag < 1:646914/141‑1 (MQ=255) gtgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGagaaga > 1:286303/1‑141 (MQ=255) gtgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACagaaga > 2:431105/1‑141 (MQ=255) gtgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACagaaga < 1:162303/141‑1 (MQ=255) tgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAgaagaa < 3:398688/141‑1 (MQ=255) ttACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACa > 1:592631/1‑141 (MQ=255) aCTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAg > 3:104084/1‑141 (MQ=255) tAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGt < 1:576897/141‑1 (MQ=255) tAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGt < 3:133253/141‑1 (MQ=255) aaaCTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTc > 1:140161/1‑141 (MQ=255) ttGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGa < 2:790890/141‑1 (MQ=255) ttGTGAGTGTGAATTTAAATAAAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGa < 2:486619/141‑1 (MQ=255) tgAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACaa > 2:379318/1‑141 (MQ=255) tgAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaa < 1:224218/141‑1 (MQ=255) tgAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaa < 3:338279/141‑1 (MQ=255) gAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaaa > 2:740644/1‑141 (MQ=255) gAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaaa > 2:740372/1‑141 (MQ=255) aGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAAt < 2:628504/141‑1 (MQ=255) gtgtGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACACATAAAGCAACTGCAGTACTTCAAGCCTTAACAGAAGAACAAGGTCAAGACAAAtt > 3:431427/1‑141 (MQ=255) gtgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTag > 2:620678/1‑141 (MQ=255) tgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTaga > 2:367081/1‑141 (MQ=255) tgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTaga > 1:590254/1‑141 (MQ=255) aaaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATaca > 1:1077313/1‑141 (MQ=255) aaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTATACGTTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCACGACAAATTAGAAGATacac > 2:83657/1‑141 (MQ=255) aaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATacac > 2:613876/1‑141 (MQ=255) aaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATacac < 1:122861/141‑1 (MQ=255) aTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATAcaca < 1:168292/141‑1 (MQ=255) aTACAATAGATGAGGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCGTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATAcaca > 4:156379/1‑141 (MQ=255) aCAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACAtt > 2:331309/1‑141 (MQ=255) cAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTc < 2:747232/141‑1 (MQ=255) aaTAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCt > 2:418131/1‑141 (MQ=255) aTAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTg < 4:190802/141‑1 (MQ=255) atgTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTc < 1:578794/141‑1 (MQ=255) atgTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGGTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTc > 3:154809/1‑141 (MQ=255) tgtgTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGATTAAAAGATAAGTTTGCAAGAAATAAAGAAAATGAAGAAGTTAAATACTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCa > 2:947898/1‑141 (MQ=255) gtTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACg > 1:402224/1‑141 (MQ=255) tGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCa < 1:485813/141‑1 (MQ=255) gAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAg < 1:1007089/141‑1 (MQ=255) gAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAg < 1:1006921/141‑1 (MQ=255) aaGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAgg > 2:847475/1‑141 (MQ=255) aggaggAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGa < 3:312064/141‑1 (MQ=255) aggaggAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGa < 1:944713/141‑1 (MQ=255) aggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGc < 2:172001/141‑1 (MQ=255) aggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGc < 1:888442/141‑1 (MQ=255) ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 3:392303/141‑1 (MQ=255) ggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCa < 3:218103/141‑1 (MQ=255) gagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCaa > 4:287613/1‑141 (MQ=255) cAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATg > 2:1012213/1‑141 (MQ=255) cAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATg < 2:636150/141‑1 (MQ=255) aatGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGAttt < 1:1023821/141‑1 (MQ=255) aatGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGAttt < 1:329207/141‑1 (MQ=255) tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg < 1:778274/141‑1 (MQ=255) tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg > 4:243661/1‑141 (MQ=255) gCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTATGAAGGTTCAACGCAGGACGCAAATGATTTAGCAg > 4:382902/1‑141 (MQ=255) cTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGa > 1:435170/1‑141 (MQ=255) ttttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaa < 4:313086/141‑1 (MQ=255) tttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaa < 2:246601/141‑1 (MQ=255) ttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaaa < 1:151863/141‑1 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg > 1:719583/1‑141 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg > 2:599893/1‑141 (MQ=255) ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg < 4:163416/141‑1 (MQ=255) tAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGc < 2:104817/141‑1 (MQ=255) aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt > 1:853086/1‑141 (MQ=255) aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt > 1:287988/1‑141 (MQ=255) aCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaaaaaaaaa < 2:658128/141‑7 (MQ=255) cGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGaa > 1:637603/1‑141 (MQ=255) tcTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCAGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAg > 2:249573/2‑141 (MQ=255) cTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGt < 2:571867/141‑1 (MQ=255) cTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGGCGCAAATGATTTAGCAGAAAATGCTGAAGt < 1:869607/141‑1 (MQ=255) aGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaaa > 1:339607/1‑141 (MQ=255) aGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaaa > 1:470079/1‑141 (MQ=255) gATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAaag < 4:30901/141‑1 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag > 4:427034/1‑141 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag > 1:447427/1‑141 (MQ=255) aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag < 1:64085/141‑1 (MQ=255) gTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGcc > 1:152161/1‑141 (MQ=255) tttGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCa > 1:28576/1‑141 (MQ=255) gCAACAAATAAAGGAAATGAAGAAGTTAAATGCTTAACAGAAGAACAAGGTAAAGAAAAATTAGAAGATACACATTCTGACGGCTCCACGCAGGATGCAAATGATTTAGCAGAAACTGCTGAAGCGCAAAAGAAGCCACgg > 4:278979/1‑140 (MQ=255) caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa > 2:803000/1‑141 (MQ=255) caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa > 2:1007214/1‑141 (MQ=255) caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa > 2:723980/1‑141 (MQ=255) aaaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGtt < 4:384052/141‑1 (MQ=255) aaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTg > 4:394908/1‑141 (MQ=255) aaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTg > 2:986809/1‑141 (MQ=255) aTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGa > 1:620032/1‑141 (MQ=255) tAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaa < 3:4285/122‑1 (MQ=255) aTGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGACGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCgg > 1:1027991/1‑141 (MQ=255) tGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGa > 2:161609/1‑141 (MQ=255) agaagTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACAATCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGAttt < 2:731156/141‑1 (MQ=255) agTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTtga < 3:90504/141‑1 (MQ=255) tAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTtgatga > 3:230534/1‑141 (MQ=255) aaTCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACg > 1:1042885/1‑141 (MQ=255) ccTTAAGCGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg > 1:859345/1‑141 (MQ=255) ccTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg > 3:373106/1‑141 (MQ=255) ccTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTGCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg < 2:190379/141‑1 (MQ=255) tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt < 1:117502/141‑1 (MQ=255) tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt < 1:133090/141‑1 (MQ=255) aaCAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCtt < 3:156088/141‑1 (MQ=255) | TCAGGTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCTT > USA300TCH1516_ALE/1250163‑1250439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |